Jean-Charles Massabuau

French National Centre for Scientific Research | CNRS · Environnements et paléoenvironnements océaniques (EPOC)

The pearl oyster Pinctada radiata is an iconic species in the Arabian Gulf, which is one of the ecosystems most at risk in the world because of the multiple sources of pollution it faces. Alongside chemical pollution, the Gulf is ranked first with regard to noise and light pollution, and pearl oyster populations are at risk. The impact of these lat...

Surveiller la qualité des eaux à l'aide de mollusques connectés    INTERVIEW par Nicolas LOUIS, posté le 15/03/2022 dans Techniques de l'Ingénieur. La station marine d'Arcachon a développé une technologie de valvométrie qui permet d'évaluer la qualité des eaux en mesurant le comportement et le bien-être de bivalves. Une start-up est sur le point...

see attached paper

The use of online remote control for 24/7 behavioural monitoring can play a key role in estimating the environmental status of aquatic ecosystems. Recording the valve activity of bivalve molluscs is a relevant approach in this context. However, a clear understanding of the underlying disturbances associated with behaviour is a key step. In this wor...

In this paper, a model-based adaptive filter is used to suppress electrical noise in a high-frequency noninvasive valvometry device, which is part of an autonomous biosensor system using bivalve mollusks valve-activity measurements for ecological monitoring purposes. The proposed model-based adaptive filter uses the dynamic regressor extension and...

Aquatic ecosystems are subject to many anthropogenic disturbances, and understanding their possible impacts is a real challenge. Developing approaches based on the behaviour of bivalve mollusks, an integrating marker of the state of the organisms, and therefore of their environment, is relevant, whether within a natural ecosystem or an ecosystem su...

Shipping has increased dramatically in recent decades and oysters can hear them. We studied the interaction between noise pollution and trace metal contamination in the oyster Magallana gigas. Four oyster-groups were studied during a 14-day exposure period. Two were exposed to cadmium in the presence of cargo ship-noise ([Cd++]w ≈ 0.5 μg∙L-1; maxim...

We present first-time ever implementation of an innovative biomonitoring technology (High-Frequency Non-Invasive Valvometry) on an offshore oil producing platform located in the Arabian Gulf (TOTAL Abu Al Bukhoosh - UAE). This technology is unique; it is a tool allowing continuous in situ monitoring of water quality and detection of hydrocarbons at...

There is an increasing concern that anthropogenic noise could have a significant impact on the marine environment, but there is still insufficient data for most invertebrates. What do they perceive? We investigated this question in oysters Magallana gigas (Crassostrea gigas) using pure tone exposures, accelerometer fixed on the oyster shell and hyd...

In this work, we study if ploidy (i.e. number of copies of chromosomes) in the oyster Crassostrea gigas may introduce differences in behavior and in its synchronization by the annual photoperiod. To answer to the question about the effect of the seasonal course of the photoperiod on the behavior of C. gigas according to its ploidy, we quantified va...

RNA interference is a powerful method to inhibit specific gene expression. Recently, silencing target genes by feeding has been successfully carried out in nematodes, insects, and small aquatic organisms. A non-invasive feeding-based RNA interference is reported here for the first time in a mollusk bivalve, the pacific oyster Crassostrea gigas. In...

As a marine organism, the oyster Crassostrea gigas inhabits a complex biotope governed by interactions between the moon and the sun cycles. We used next-generation sequencing to investigate temporal regulation of oysters under light/dark entrainment and the impact of harmful algal exposure. We found that ≈6% of the gills’ transcriptome exhibits cir...

Marine ecosystems all over the globe are facing multiple simultaneous stressors including rapid climatic change and increased resource exploitation, such as fishing, petroleum exploration and shipping. The EU-funded DEVOTES project (DEVelopment Of innovative Tools for understanding marine biodiversity and assessing good Environmental Status) aims t...

Although the prevailing paradigm has held that the polar night is a period of biological quiescence, recent studies have detected noticeable activity levels in marine organisms. In this study, we investigated the circadian rhythm of the scallop Chlamys islandica by continuously recording the animal’s behaviour over 3 years in the Arctic (Svalbard)....

Cryptochromes are flavin- and pterin-containing photoreceptors of the cryptochrome/photolyase family. They play critical roles in organisms, among are which light-dependent and light-independent roles in biological rhythms. The present work aimed at describing a cryptochrome gene in the oyster Crassostrea gigas by (i) a characterization and phyloge...

As a marine organism, the oyster Crassostrea gigas inhabits a complex biotope governed by interactions between the moon and the sun cycles. We used next-generation sequencing to investigate temporal regulation of oysters under light/dark entrainment and the impact of harmful algal exposure. We found that ≈6% of the gills’ transcriptome exhibits cir...

The precise environmental conditions under which broadcast spawners spawn in the field remain largely unknown. We investigated this issue in the oyster Crassostrea gigas using three different methods at different time scales in two traditional oyster farming areas of the French Atlantic Coast, the Bay of Arcachon and Marennes-Oléron. We directly re...

Using measurements of valve activity (i.e., the distance between the two valves) in populations of bivalves under natural environmental conditions (16 oysters in the Bay of Arcachon, France, in 2007, 2013, and 2014), an algorithm for an automatic detection of the spawning period of oysters is proposed in this brief. Spawning observations are import...

The measurements of valve activity in a population of bivalves under natural environmental conditions (16 oysters in the Bay of Arcachon, France) are used for a physiological model identification. A nonlinear auto-regressive exogenous (NARX) model is designed and tested. The method to design the model has two parts. 1) Structure of the model: The m...

Adequate and efficient environmental monitoring is a key element of the environmental risk reduction process in the Oil & Gas industry, all the more where sensitive areas in harsh environmental conditions are at stake. This is the case in remote and extreme cold places such as the Arctic, where robust systems are required to withstand adverse clima...

Адекватный и эффективный мониторинг окружающей среды является ключевым элементом в процессе уменьшения экологического риска в области нефте- и газоиндустрии, особенно там где вопрос стоит о чувствительных экосистемах, находящихся в суровых условиях. К таким условиям относятся удаленные и экстремальные холодные районы Арктики, где устойчивые монитор...

The current understanding of Arctic ecosystems is deeply rooted in the classical view of a bottom-up controlled system with strong physical forcing and seasonality in primary-production regimes. Consequently, the Arctic polar night is commonly disregarded as a time of year when biological activities are reduced to a minimum due to a reduced food su...

Using measurements of valve activity in a population of bivalves under natural environmental condition (16 oysters in the Bay of Arcachon, France), an algorithm for the automatic detection of spawning period of oysters is proposed. The algorithm is based on the fault detection approach and it works through the estimation of velocity of valves movem...

The measurements of valve opening activity in a population of oysters under natural environmental conditions are used to estimate the velocity of their valve movement activity. Three different differentiation schemes were used to estimate the velocity, namely an algebraicbased differentiator method, a non-homogeneous higher order sliding mode diffe...

The present study investigates cadmium effects on the transcription of mitochondrial genes of Procambarus clarkii after acute (0.05, 0.5, and 5 mg Cd/L; 4-10 days) and chronic exposures (10 μg Cd/L; 30-60 days). Transcriptional responses of cox1, atp6, and 12S using quantitative real-time RT-PCR were assessed in gills and hepatopancreas. Additional...

The measurements of valve activity in a population of bivalves under natural environmental conditions (16 oysters in the Bay of Arcachon, France) are used for a physiological model identification. A nonlinear auto-regressive exogenous (NARX) model is designed and tested. The model takes into account the influence of environmental conditions using m...

Valve activity rhythm of the oyster Crassostrea gigas is mainly driven by tides in the field, but in the laboratory, only a circadian clock mechanism has been demonstrated. In an attempt to reconcile these results, the mechanisms underlying the circatidal rhythm were studied in the laboratory under different entrainment or free-running regimes and...

MOLLUSCAN EYE, AN AUTONOMOUS UNMANNED SYSTEM FOR LONG TERM RECORDINGS OF BIVALVE BEHAVIOR FROM THE POLE TO THE EQUATOR. The MolluSCAN Eye project makes use of the bivalve shell movements and the m2m world to monitor in near real-time water quality changes anywhere there is a cellular network or Ethernet socket. The project began in 2006 in France...

Genotoxic, genetic and behavioral impacts of the paralytic shellfish toxin (PST)-producing alga Alexandrium minutum on the oyster Crassostrea gigas were assessed using RAPD-PCR, qPCR and valve activity recording. Oysters were exposed to a dose mimicking an algal bloom (≈1600cellsml(-1)) for 48h. Results indicate a rapid and sustained behavioral dis...

Les rythmes biologiques permettent aux organismes de mesurer le temps et d’anticiper les changements cycliques de leur environnement. Si les espèces terrestres sont essentiellement soumises au cycle solaire d’alternance jour/nuit, les espèces marines occupent un environnement beaucoup plus complexe, fortement influencé également par plusieurs cycle...

Although a significant body of literature has been devoted to the chronobiology of aquatic animals, how biological rhythms function in molluscan bivalves has been poorly studied. The first objective of this study was to determine whether an endogenous circadian rhythm does exist in the oyster, Crassostrea gigas. The second objective was to characte...

Previous in vitro and in vivo studies showed that the frequency of rhythmic pyloric network activity in the lobster is modulated directly by oxygen partial pressure (PO(2)). We have extended these results by (1) increasing the period of exposure to low PO(2) and by (2) testing the sensitivity of the pyloric network to changes in PO(2) that are with...

The visual system is particularly sensitive to methylmercury (MeHg) exposure and, therefore, provides a useful model for investigating the fundamental mechanisms that direct toxic effects. During a period of 70 days, adult of a freshwater fish species Hoplias malabaricus were fed with fish prey previously labeled with two different doses of methylm...

We examine the physiological and lifestyle adaptations which facilitated the emergence of ostracods as the numerically dominant Phanerozoic bivalve arthropod micro-benthos. The PO(2) of modern normoxic seawater is 21 kPa (air-equilibrated water), a level that would cause cellular damage if found in the tissues of ostracods and much other marine fau...

We consider bivalve (oyster) behavior using high frequency valvometry data. These data are obtained in the field in the Arcachon bay, with light-weight electrodes of millimeters size, attached to the molluscs, and linked by flexible wires to an electronic control unit which measures valve activity. The latter are able to move freely and open their...

Oxygen fostered life but it is also one of the most hazardous cellular toxicants. Oxidative stress occurs when the production ofreactive oxygen species (ROS) exceeds the antioxidant capacities. Too much free unbound oxygen in the cellular environment is deleterious, and one of the primary ways to prevent ROS from forming is to keep tissue oxygen ac...

The high-frequency measurements of valve activity in bivalves (e.g., valvometry) over a long period of time and in various environmental conditions allow a very accurate study of their behaviors as well as a global analysis of possible perturbations due to the environment. Valvometry uses the bivalve's ability to close its shell when exposed to a c...

The life history of 15 giant clams, Hippopus hippopus, was studied in situ in the southern lagoon of New Caledonia; growth rate and animal behavior were studied both by sclerochronology and high-frequency noninvasive (HFNI) valvometry. Electrodes glued on each valve of each specimen recorded the shell-gaping behavior at 0.6-Hz frequency. A nonparam...

Behaviour represents a reaction to the environment as fish perceive it and is therefore a key element of fish welfare. This review summarises the main findings on how behavioural changes have been used to assess welfare in farmed fish, using both functional and feeling-based approaches. Changes in foraging behaviour, ventilatory activity, aggressio...

Recovery from caudal artery cannulation with and without pre-anaesthesia metomidate sedation was assessed in Atlantic cod (Gadus morhua). The levels of plasma cortisol, glucose, electrolytes and acid-base parameters were compared between sedated and unsedated cod and to those in uncannulated individuals, where the samples were obtained by sacrifici...

The complexity of the relationships between Alexandrium minutum (A.m.) concentration in the water ([A.m.](w)), Paralytic Shellfish Poisoning contamination in the digestive gland ([PSP](dg)) and valve behavior was explored in oysters Crassostrea gigas. Two experiments were conducted, during which oysters' valve behaviour were analyzed. Oysters, firs...

The present study reports new insights into the complexity of environmental drivers in aquatic animals. The focus of this study was to determine the main forces that drive mollusc bivalve behavior in situ. To answer this question, the authors continuously studied the valve movements of permanently immersed oysters, Crassostrea gigas, during a 1-yea...

The basic adaptation mechanisms that allow the Asian freshwater clam Corbicula fluminea to maintain its oxygen (O2) consumption constant under resting conditions when the partial pressure of O2 () in the water varies from 4 to 40 kPa were studied at plankton concentrations which were high enough that ventilation was not affected. Steady-state value...

The blood oxygen status of two species of active crabs (Carcinus maenas and Necora puber) was studied in the field and compared with the results of previous laboratory experiments performed on a wide spectrum of physiologically different water-breathers. The aim was to determine whether, as in the laboratory, the functioning of the O2 supply system...

Numerous resting unfed water-breathers have a strategy of gas-exchange regulation that consists of setting the arterial partial pressure of oxygen (Pao2) at 1-3 kPa. This raises a question concerning the extent to which physiological functions are limited in this situation. To obtain insight into this problem, we studied the steady-state adaptation...

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Methylmercury is a known neurotoxic organometal which affects visual functions and few studies concerns to wild fish are available. The autometallography mercury distribution in the retina of Danio rerio was mapped using light and electron microscopy. Abundant mercury deposits were found in the photoreceptor layer (outer and inner segments of the p...

Regulation of arterial partial pressure of O2 (PaO2) in Atlantic salmon (Salmo salar) was investigated during resting conditions in normoxic and hyperoxic water. Dorsal aorta cannulated adult Atlantic salmon (1.2-1.6 kg, n=8) were exposed to 2 week sequential periods of normoxia [16.7±1.1 kPa (mean±SD)] and hyperoxia (34.1±4.9 kPa) in individual ta...

We describe the valve-activity behavior of oysters, Crassostrea gigas, exposed experimentally to the harmful alga Alexandrium minutum (≈ 3500 cell ml− 1) for 7-day periods under laboratory conditions. Our aim was to assess behavioral responses of oyster species during a mimicked bloom exposure. We determined different characteristic parameters of v...

The life history of 15 giant clams, Hippopus hippopus, was studied in situ in the southern lagoon of New Caledonia; growth rate and animal behavior were studied both by sclerochronology and high-frequency noninvasive (HFNI) valvometry. Electrodes glued on each valve of each specimen recorded the shell-gaping behavior at 0.6-Hz frequency. A nonparam...

European eels are dangerously threatened with extinction. Recent advances tend to show that pollution could, in addition to other already identified factors, contribute to this drama. In a previous report, cadmium (Cd) pre-exposure was found to strongly stimulate the pituitary-liver-gonad axis of maturing female silver eels, leading, lastly, to ooc...

Selenium is a trace element characterized by concentrations that narrowly range between being essential and being toxic. Even though inorganic selenite and selenate are the predominantchemicalforms ofSe in surfacewaters, the toxicity of Se to aquatic organisms is mostly governed by the bioavailability of organic selenium within food webs. The prese...

The European eel (Anguilla anguilla L.) is severely threatened with extinction. Surprisingly, even though their unusual life cycle makes them particularly vulnerable to pollution, the possible contribution of contamination remains especially poorly known. Here we have investigated the possible effect of cadmium (Cd), a widespread nonessential metal...

Multi-stress situations are a major question and low-oxygenated waters (hypoxia) are a growing problem. Importantly, hypoxia stimulates the ventilatory flow rate in aquatic animals and this increases gill exposure to contaminants. Surprisingly, in the freshwater clam Corbicula fluminea, this is associated with increased bioaccumulation of uranium i...

Due to its status of threatened species and being heavily contaminated by metals, the European eel (Anguilla anguilla) was selected to investigate cadmium contamination levels of fish settled along a historically cadmium-contaminated hydrosystem, the Garonne-Gironde continuum (France), according to its various location sites and fish length. Result...

The aim of this study was to characterize the respiratory activity (O2 consumption, O2 haemolymph status, cardiac-flow rate) of C. gigas cultivated in Thau lagoon water. The effects of season (summer and winter), sex and gametogenic cycle (pre-/post-spawning and resting status) were studied. Oxygen consumption was directly influenced by temperature...

Mercury and more specifically methylmercury have been reported as hazardous environmental pollutants able to accumulate along the aquatic food chain with severe risk for animal and human health. Adult male zebrafish (Danio rerio) were distributed in two groups: a control group (fed with uncontaminated food) and a MeHg-contaminated group (fed with f...

The underlying physiological mechanisms explaining why the adult penaeid prawn Litopenaeus stylirostris cannot successfully face heavy stressful events on the low edge of its thermopreferendum (20–22°C) were studied during the austral winter. Prawns were studied during recovery from net fishing and rapid transfer from outdoor earthen ponds into ind...

The aim of this study was to investigate how the worm Polydora sp., which induces oysters into creating mud blisters in response to an irritation within their shells, could interfere with the oyster Crassostrea gigas physiology and ethology. The impact was characterized by studying two groups of oysters (non-parasitized and parasitized) during a 30...

The objective of the present study was to monitor water-quality assessment by a biological method. Optimum dissolved inorganic mercury sensitivity in the freshwater bivalve Corbicula fluminea was estimated using a combined approach to determine their potentials and limits in detecting contaminants. Detection by bivalves is based on shell closure, a...

The aim of the present study was to investigate the role of two key environmental factors of estuarine ecosystems, salinity and hypoxia, on the Cd bioaccumulation by direct exposure in the white shrimp Palaemon longirostris. Two types of experiments were performed in the laboratory. First, we studied Cd accumulation by shrimp after metal exposure a...

European eel (Anguilla anguilla) populations are in decline. Glass eel recruitment has fallen 10-fold since the early 1980s. Estuaries play a fundamental role in the life history of eels because glass eels must pass through them to reach freshwater ecosystems. Unfortunately, because of their geographical position at the upstream basin slopes, estua...

Because European silver eels (Anguilla anguilla) fast during their reproductive migration to the Sargasso Sea, the successful completion of their unusual life cycle depends on quantity of lipids stored beforehand. These lipids are mainly accumulated during the growth phase stage of the animals, called yellow eel, as triglycerides in muscle. They ar...