Yoshitsugu Inoue's research while affiliated with Tottori University and other places
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Publications (162)
To estimate the roles of extracellular vesicles (EVs) in tears and to determine whether their profiles are associated with the type of ocular disease.
Cross-sectional study.
Tear EVs were extracted from 14 healthy participants and from 21 patients with retinal diseases (age-related macular degeneration [AMD] or diabetic macular edema [DME]). The su...
Purpose: To determine whether there is a significant association between the inflammatory cytokines in the tear fluid and the severity of the Retinopathy of Prematurity Severity (ROP).
Study Design: Retrospective cohort study.
Methods: The cytokine levels in tear fluids were determined in 34 eyes with ROP and 18 eyes without ROP. There were 15 eyes...
The purpose of this study was to determine whether there is a significant association between the level of cytokines in the tear fluid and the presence and severity of the Retinopathy of prematurity (ROP). The level of the cytokines was determined in 34 eyes of 34 infants with ROP and 18 eyes of 18 infants without ROP. There were 15 eyes of 15 seve...
Purpose:
To determine the effect of the formulation of topical medications on the healing of corneal epithelial cells after phototherapeutic keratectomy (PTK).
Study design:
Retrospective cohort study.
Methods:
We studied 271 eyes of 189 consecutive patients (aged 67.6 ± 11.8 years) who had undergone PTK for granular corneal dystrophy (n = 140...
Kazuki Matsuura,1,2 Yoshitsugu Inoue2,3 1Nojima Hospital, Kurayoshi-city, Tottori, 6820863, Japan; 2Tottori University, Yonago-city, Tottori, 6838504, Japan; 3Hino Hospital, Hino-gun, Tottori, 6894504, JapanCorrespondence: Kazuki Matsuura, Nojima Hospital, 2714-1, Sesaki-machi, Kurayoshi-city, Tottori, 6820863, Japan, Tel +81-858-22-6231, Fax +81-8...
The purpose of this study was to identify the inflammatory cytokines that were associated with pachychoroid neovasculopathy (PNV). Seventy-five eyes of 75 patients with PNV, 145 eyes of 145 patients with neovascular age-related macular degeneration without pachyvessels, and 150 eyes of 150 normal subjects were examined for the levels of intraocular...
Rhabdophis tigrinus ( R. tigrinus ) is a common colubrid snake that possesses a series of paired sac-like nuchal glands behind the head. When pressure is applied to the nuchal area, the thin skin over the nuchal glands can rupture and release secretions. In Japan, 19 cases of ophthalmia caused by the nuchal gland secretion of R. tigrinus have been...
PurposeTo gain new insights into the etiology of blepharitis, we investigated the causative bacteria in patients with blepharitis and the effects of 1% azithromycin ophthalmic solution.Study designA multicenter, prospective observational study.Methods
After the subjects were diagnosed as having blepharitis they were administered 1% azithromycin oph...
The purpose of this study was to identify inflammatory cytokines that are associated with pachychoroid neovasculopathy (PNV). Seventy-five eyes of 75 patients with PNV, 145 eyes of 145 patients with neovascular age-related macular degeneration without pachyvessels, and 150 eyes of 150 normal subjects were examined for the levels of intracameral cyt...
Ocular cytomegalovirus (CMV) infections in immunocompetent individuals are rare, but its activation can cause chronic and relapsing inflammation in anterior segment of the eye resulting in loss of corneal clarity and glaucoma. Fifty five patients with anterior segment CMV infection were assessed for their clinical characteristics, and CMV corneal e...
Purpose:
To determine the effects of a combination of two antifungal drugs against causative fungi of fungal keratitis in Japan.
Study design:
Multicenter prospective observational study.
Methods:
Eighteen isolates of yeast-like fungi and 22 isolates of filamentous fungi collected by the Multicenter Prospective Observational Study of Fungal Ke...
Purpose:
To investigate the clinical characteristics and causative fungi in patients with fungal keratitis in Japan, and to determine factors related to the prognosis.
Study design:
Multicenter prospective observational study.
Methods:
Eligible patients were enrolled from November 2011 to October 2013 at the 1st stage and from April 2015 to Ma...
Background:
There are no reports on accurate measurement of lens equatorial diameter of the living human eye. This study aimed to measure lens equatorial diameter with a special measurement device during cataract surgery and examine the relationships with preoperative parameters.
Methods:
From April 7 to December 1, 2019, the equatorial diameter...
Corneal opacities are important causes of blindness, and their major etiology is infectious keratitis. Slit-lamp examinations are commonly used to determine the causative pathogen; however, their diagnostic accuracy is low even for experienced ophthalmologists. To characterize the “face” of an infected cornea, we have adapted a deep learning archit...
Corneal opacities are an important cause of blindness, and its major etiology is infectious keratitis. Slit-lamp examinations are commonly used to determine the causative pathogen; however, their diagnostic accuracy is low even for experienced ophthalmologists. To characterize the “face” of an infected cornea, we have adapted a deep learning archit...
Viral infections of the cornea including herpes simplex virus 1 (HSV-1) cause visual morbidity, and the corneal endothelial cell damage leads to significant visual impairment. Interferon regulatory factor 7 (IRF7) has been identified as a significant regulator in corneal endothelial cells after an HSV-1 infection. To examine the role played by IRF7...
Senescence, sterile inflammation, and infection cause dysfunction of corneal endothelial cells, leading to visual morbidity that may require corneal transplantation. With increasing age, the extracellular matrix is modified by non-enzymatic glycation forming advanced glycation end products (AGEs). The modifications are primarily sensed by the recep...
Viral infections of the cornea including herpes simplex virus 1 (HSV-1) cause visual morbidity, and the corneal endothelial cell damage leads to significant visual impairment. Interferon regulatory factor 7 (IRF7) has been identified as a significant regulator in corneal endothelial cells after an HSV-1 infection. To examine the role played by IRF7...
To understand the clinical course of human adenoviral (HAdV) conjunctivitis for establishing a better treatment regimen, thirty-eight eyes of 19 patients with HAdV-54 conjunctivitis for less than one week from onset were evaluated for clinical signs and symptoms and DNA copy numbers. It is observed that a viral load of 10⁴–10⁵ is required to develo...
Purpose
To determine distinguishing features of the clinical characteristics of anterior uveitis (AU) caused by herpes simplex virus (HSV), varicella-zoster virus (VZV) and cytomegalovirus (CMV).
Design
Retrospective, multicenter, case series.
Methods
Consecutive patients with herpetic AU examined at 11 tertiary centers in Japan between January 2...
PurposeTo analyze the epidemiologic characteristics of an outbreak of human adenovirus type 54 (HAdV-54) on Oki Island, Shimane Prefecture, Japan, in 2017 and to assess the effectiveness of a compartmentalization method in controlling the incidence and spread.Study designRetrospective cohort study.Methods
The infection was diagnosed in 136 individu...
PurposeThe aim of this study was to compare the efficacy of the topical regimens of iodine/steroids vs. antibiotics/steroids in acute to subacute adenoviral keratoconjunctivitis.Study designA prospective open-label study.Patients and methodsNineteen patients diagnosed with unilateral or bilateral adenoviral conjunctivitis at less than 1 week from o...
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Surgically induced scleritis (SIS) is a rare complication after a variety of ocular surgeries. Majority of the patients had had two or more surgical procedures before the onset of SIS. We report a patient who developed bilateral diffuse scleritis after unilateral cataract surgery.
A 64-year-old man presented with red eye and tenderness in the righ...
Purpose:
To determine whether topical tacrolimus can lessen steroid-induced intraocular pressure (IOP) elevation.
Study design:
Open cohort post hoc analysis study.
Methods:
Five hundred eleven patients with vernal keratoconjunctivitis or atopic keratoconjunctivitis (mean age 17.0 ± 9.2 years) were studied. All 511 patients were treated with t...
Acanthamoeba can cause visually destructive Acanthamoeba keratitis (AK) in contact lens (CL) users. The purpose of this study was to determine whether Acanthamoeba was present in the CL cases of CL wearers and to develop techniques to prevent the contaminations. To accomplish this, 512 CL case samples were collected from 305 healthy CL wearers. Usi...
Age-associated sterile inflammation can cause dysregulated choroidal neovascularization (CNV) as age-related macular degeneration (AMD). Intraocular fluid screening of 234 AMD patients identified high levels of IL-4. The purpose of this study was to determine the functional role of IL-4 in CNV formation using murine CNV model. Our results indicate...
Age-associated sterile inflammation can cause dysregulated choroidal neovascularization (CNV) as age-related macular degeneration (AMD). Intraocular fluid screening of 234 AMD patients identified high levels of IL-4. The purpose of this study was to determine the functional role of IL-4 in CNV formation using murine CNV model. Our results indicate...
Age-associated sterile inflammation can cause dysregulated choroidal neovascularization (CNV) as age-related macular degeneration (AMD). Intraocular fluid screening of 234 AMD patients identified high levels of IL-4. The purpose of this study was to determine the functional role of IL-4 in CNV formation using murine CNV model. Our results indicate...
PurposeTo optimize prophylactic antibiotic administration, antibiotic susceptibility before cataract surgery was investigated using ocular bacteria isolated preoperatively.DesignRetrospective cross-sectional study.Methods
In 204 eyes of 102 patients who underwent routine bilateral cataract surgery, conjunctival sac scrapings were collected 1–2 week...
Purpose
To evaluate the efficacy of real-time PCR for 16S ribosomal DNA (16S r-DNA) and sequencing for diagnosing microbial keratitis.
Methods
We studied 272 eyes of 272 patients with keratitis. Eyes with keratitis were classified as “definite” (N = 118), “likely” (N = 71), or “non-bacterial” (N = 83) to have bacterial keratitis. The diagnostic ef...
Ambient air pollution is a well-recognized risk for various diseases including asthma and heart diseases. However, it remains unclear whether air pollution may also be a risk of ocular allergic diseases. Using a web-based, nation-wide, cross-sectional study design, we examined whether the level of ambient air pollution is significantly associated w...
Purpose
To verify the possibility that preoperative topical antibiotics are not essential as long as iodine disinfection is performed during surgery.
Study design
Crossover equivalence trial.
Patients and methods
In 204 eyes of 102 patients who underwent routine bilateral cataract surgery, 1 eye was treated with intraoperative iodine, and the oth...
Prophylactic intracameral injection of antibiotics is commonly used to prevent endophthalmitis after cataract surgery. However, devastating visual complications have been reported including hemorrhagic occlusive retinal vasculitis (HORV).To determine the toxic and inflammatory effects of moxifloxacin, cefuroxime, and vancomycin on human retinal vas...
Background
Of the 10 patients with adenoviral type 54 keratoconjunctivitis examined at Nojima Hospital, 2 developed stellate keratitis and mutton-fat keratic precipitates (KPs) following acute symptoms.
Case presentation
We encountered 10 cases of epidemic keratoconjunctivitis from August to October 2017. All patients were adults with a mean age o...
Purpose
The aim of this study is to determine the efficacy of quantitative real-time PCR (qPCR) and clinical characteristics to diagnose ocular cytomegalovirus (CMV) infections.
Methods
The technical factors were assessed by the outcomes of the qPCR assay at five institutions in Japan using the WHO International Standard of cytomegalovirus. The cl...
Glaucoma is a leading cause of blindness worldwide. Purpose of this study was to identify molecular markers that were significantly correlated with presence of glaucoma and outcome of glaucoma surgery. To accomplish this, we determined the profiles of pro-inflammatory cytokines in the aqueous humor of 101 glaucoma patients; 31 primary open angle gl...
Purpose:
Human cytomegalovirus (HCMV) infections can cause endotheliitis which is associated with an elevation in the intraocular pressure (IOP). However, the mechanism of the IOP elevation has not been established. The purpose of this study was to determine whether HCMV strains which are capable of infecting corneal endothelial cells can also rep...
Purpose:
To survey the demographics, risk factors, microbiology and outcomes for infectious keratitis in Asia.
Design:
Prospective, non-randomized clinical study METHODS: Thirteen study centers and 30 sub-centers recruited consecutive subjects over 12 - 18 months, and performed standardized data collection. A microbiological protocol standardize...
Purpose:
To determine the efficacy of real-time PCR for the diagnosis and prognosis of varicella-zoster virus (VZV) keratitis.
Study design:
Retrospective case series.
Methods:
Patients: 545 consecutive patients with keratitis were examined to quantify copy numbers of VZV DNA by real-time PCR. Association of copy numbers of VZV DNA to clinical...
Infection of the corneal endothelial cells by human cytomegalovirus (CMV) is an important cause of corneal endotheliitis. CMV endotheliitis is difficult to completely cure and relapses are frequent. This can cause blinding corneal bullous keratopathy. However, the pathogenesis of CMV endotheliitis remains undetermined. To understand the immunopatho...
Background
Surgeons may be unaware of the ability of plant toxins to cause corneal damage. Therefore, corneal damage following intraocular surgery due to plant toxins may be misdiagnosed as postoperative infection. Case presentationA 74-year-old man presented with hyperemia and reduced visual acuity in both eyes 6 weeks after uneventful cataract su...
Purpose
The purpose of this study was to evaluate the clinical utility of SG17, an ophthalmic antimicrobial susceptibility measurement plate.
Design
This was a multicenter, retrospective, observational study.
Patients and methods
Using clinical isolates from patients with ocular infections, drug susceptibility testing using the Clinical Laborator...
PurposeTo determine the antiseptic efficacy of timely intraoperative iodine irrigation during cataract surgery. MethodsA total of 198 eyes of 99 cataract surgery patients were studied. The eyes were randomly assigned to treatment with or without timely intraoperative iodine irrigation of the surgical field with an iodine compound equivalent to 0.33...
Background/aims The aim of this study was to evaluate the therapeutic efficacy and drug transfer of topical application of 0.15% ganciclovir (GCV) gel on cytomegalovirus (CMV) corneal endotheliitis.
Methods This study is a multicentre, prospective, interventional case series. Seven eyes of seven immunocompetent patients diagnosed with CMV corneal e...
Purpose:
To report a case of unilateral retinal pigment epithelium dysgenesis.
Methods:
An 8-year-old boy with a large grayish lesion and leopard-spot pattern lesion at its periphery in his left eye underwent fluorescein angiography, fundus autofluorescence imaging, optical coherence tomography, and electroretinography.
Results:
Fluorescein an...
Corneal endothelial cells are known to be targets of herpes simplex virus type 1 (HSV-1) infection; however, the pathogenesis of HSV infections of the endothelial cells has not been definitively determined. The purpose of this study was to examine an unrecognised strategy of corneal endothelial cells to protect themselves from HSV-1 infection.
Immo...
We developed an immunochromatographic assay kit that uses fluorescent silica nanoparticles bound to anti-Acanthamoeba antibodies (fluorescent immunochromatographic assay [FICGA]) and evaluated its efficacy for the detection of Acanthamoeba and diagnosis of Acanthamoeba keratitis (AK). The sensitivity of the FICGA kit was evaluated using samples of...
Purpose:
To determine the relationships between the levels of intraocular inflammatory cytokines and the clinical characteristics of myopic choroidal neovascularization (mCNV) in eyes with myopic maculopathy.
Methods:
One hundred eyes of 100 cases, including 51 mCNV eyes, 14 highly myopic eyes without choroidal neovascularization, and 35 normal...
Purpose
To elucidate Japanese trends for perioperative disinfection and antibiotic selection during cataract surgeries.
Methods
Perioperative iodine use and antibiotic prophylaxis for cataract surgery were surveyed in eight regions in Japan by mail or through interviews from February 1 to March 1, 2014.
Results
We surveyed 572 surgeons, of whom 3...
Purpose:
To evaluate and compare the toxic effects of moxifloxacin, cefuroxime, and levofloxacin on human corneal endothelial cells in vitro and determine the safe intracameral concentrations for them.
Setting:
Tottori University, Tottori, Japan.
Design:
Experimental study.
Methods:
Human corneal endothelial cells in culture were exposed to...
Purpose
To report the first multicenter survey in Japan on infectious keratitis after excimer laser surgery.
Methods
The laser in situ keratomileusis (LASIK) Safety Network (LSN) Committee sent questionnaires to 28 LSN member hospitals to assess the total number of laser corneal surgeries, the number of infection cases (including suspicious cases)...
: Corneal endotheliitis was first reported as an autoimmune disease by Khodadoust in 1982. Thereafter, herpes simplex virus (HSV) was detected as a causative agent, and corneal endotheliitis was classified as an infectious disease. However, most primary corneal endotheliitis had been idiopathic. In 2006, cytomegalovirus (CMV) DNA was detected in a...
Abstract Purpose: To assess the safety of intracameral injection of moxifloxacin (MFLX) using the total replacement technique [bag and chamber (BC) flushing]. Methods: The anterior chamber including the area behind the intraocular lens was irrigated and replaced using BC flushing. In Group A, 36 patients received balanced salt solution irrigation i...
Aims
The purpose of this study is to elucidate the clinical manifestations and the current treatment status of cytomegalovirus (CMV) endotheliitis via a large case series obtained from a national survey conducted in Japan.
Methods
The Japan Corneal Endotheliitis Study Group proposed diagnostic criteria for CMV endotheliitis based on a viral examin...
Purpose:
To evaluate the effectiveness of topical agents for the treatment of Acanthamoeba keratitis (AK).
Design:
Laboratory research.
Participants:
Fifty-six Acanthamoeba isolates from 56 patients with clinically proven AK were studied.
Methods:
The effectiveness of 7 agents against Acanthamoeba cysts was determined in vitro. The agents we...
While corneal endothelial damage in its most advanced stage results in bullous keratopathy where corneal transplantation is needed, there is no severity grading for corneal endothelial damage. The Japanese Corneal Society formed a working group to establish a severity grading for corneal endothelial damage. The following are the proposed severity g...
The purpose of this study was to assess the risk of intraocular contamination caused by intraocular lens (IOL) insertion with injectors by observing the dynamics of an ophthalmic viscosurgical device (OVD).
Each type of injector was equipped with a colored OVD and IOL, and a 2 mm length from the tip of the cartridge was replaced with a colored OVD....
To report the isolation of Pestalotiopsis clavispora from the cornea of a patient with recurrent keratitis.
A 73-year-old male gardener presented with conjunctival injection and an oval infiltrate with feathery margins in the temporal half of the cornea in the right eye. His ocular history in the right eye included cataract surgery, five episodes o...
To report endophthalmitis rates after cataract surgery and the incidence of complications after intracameral moxifloxacin injection.
Nineteen clinics in Japanese institutions.
Retrospective survey cohort study.
The number of surgeries and endophthalmitis cases in the past 4 years before and after the introduction of intracameral moxifloxacin was ev...
To characterize the cytomegalovirus-associated anterior segment inflammation and to determine whether the number of cytomegalovirus is significantly correlated with the disease characteristics.
Retrospective consecutive case series. Seventy-three patients with refractory anterior segment inflammation due to iridocyclitis, corneal endotheliitis and...
Posterior capsule rupture causes instant vitreous contamination, resulting in endophthalmitis. However, transfer of intracameral moxifloxacin (MFLX) to the vitreous has not been examined in detail. We investigated vitreous antibiotic concentrations following intracameral MFLX in both ruptured and intact posterior capsular eyes.
Experiment 1: Intrao...
Pharmacologic inhibitors of IκB kinase (IKK), especially IKK-β, have been developed to treat inflammatory diseases. However, their interactions with components of the NF-κB pathways are not fully known in allergic diseases. To examine whether IKK is involved in immediate hypersensitivity reactions and to determine whether counterregulatory mechanis...
Background:
Studies have indicated that intracameral administration of moxifloxacin (MFLX), a fourth-generation fluoroquinolone, is safe and effective. However, administration methods vary between studies, and no definite protocol exists. A prospective study clarifying the incidence of endophthalmitis and complication rates associated with each ad...
To evaluate the efficacy of Gram-Fungiflora Y double staining for corneal scraping samples collected by impression in diagnosing infectious keratitis.
Two hundred and thirteen eyes of 192 patients suspected of having infectious keratitis were retrospectively studied for the sensitivity and specificity of Gram-Fungiflora Y based on final diagnosis....
Aims:
To determine the efficacy of a new helicase-primase inhibitor, ASP2151, for treating herpetic keratitis.
Methods:
Murine corneas were infected with herpes simplex virus type 1 (HSV-1). ASP2151 was administered orally or topically, and the severity of epithelial dendritic keratitis was determined. The effectiveness of ASP2151 was compared w...
Background/aims:
The novel immunochromatographic assay (ICGA) kit was recently developed to diagnose herpes simplex virus (HSV) infection. This multicentre study aimed to evaluate the value of the ICGA kit for the diagnosis of herpetic epithelial keratitis by comparing it with immunofluorescence assay (IFA) and real-time PCR.
Methods:
Corneal sc...
The objective of this study was to identify trends in the use of perioperative antibiotic prophylaxis in cataract surgery in Japan.
This was a national survey by mail or interview.
The use of perioperative antibiotic prophylaxis in cataract surgery was evaluated by conducting a survey in 6 regions within Japan from October 1, 2011, to March 1, 2012...
Background:
Intracameral moxifloxacin is currently administered by injecting small doses (0.05-0.2 mL) of either undiluted or diluted solutions. It is difficult to ensure delivery of small amounts of antibiotic into the area behind the intraocular lens (IOL). Moreover, the anterior chamber pressure decreases as the tip of irrigation is removed, of...
To determine the relationship between the levels of intraocular inflammatory cytokines and polypoidal choroidal vasculopathy (PCV).
Prospective cohort study. Sixty-two patients with PCV and 36 control subjects were studied. The levels of cytokines, chemokines, and growth factors in the aqueous humor samples from PCV patients and control subjects we...
To evaluate the diagnostic value of real-time polymerase chain reaction (PCR) for detecting Acanthamoeba in eyes diagnosed with Acanthamoeba keratitis (AK) by conventional tests. In addition, to determine the preoperative prognosis-determining factors in eyes with AK.
Retrospective, cross-sectional study.
A total of 104 eyes of 103 patients who wer...
To report the drug sensitivity of causative agents produced by ocular infection of external adnexa and anterior segments investigated by the nationwide survey conducted by Japanese Association for Ocular Infection between September, 2007 and August, 2008.
Among all strains isolated, causative and presumed causative agents were selected according to...
To determine the causative agents of ocular infection of external adnexa and anterior segments due to selected criteria.
Between September 22, 2007 and August 25, 2008, 890 bacterial strains were collected from 476 patients in 18 facilities nationwide participating in the Drug Sensitivity for Ocular Infection Study Group. Usual aerobic and anaerobi...
To determine the roles played by toll-like receptor 9 (TLR9) in cultured human corneal endothelial (HCEn) cells after herpes simplex virus type 1 (HSV-1) infection and to characterize the TLR9-mediated antiviral responses.
Immortalized HCEn cells were examined for TLR expression. The upregulation of inflammatory cytokines after HSV-1 infection was...
To determine the transcriptional response of cultured human corneal endothelial (HCEn) cells after herpes simplex virus type (HSV-1) infection and to characterize the primary functional elements and antiviral responses.
Immortalized HCEn cells were infected with HSV-1, and the global transcriptional profile was determined. The transcriptional netwo...
To characterize the differential expression of intraocular inflammatory cytokines in eyes with branch retinal vein occlusion (BRVO) and to assess their roles as prognostic determinants of BRVO.
A prospective cohort study of 38 eyes with BRVO. Aqueous humor samples were collected just before the intravitreal injection of bevacizumab and were assesse...
To understand the current state of severe contact lens (CL)-associated microbial keratitis in Japan.
The survey was conducted by the Japan Contact Lens Society and the Japanese Association for Ocular Infection in 224 facilities from April 2007 to March 2009. Patients who were diagnosed with CL-associated microbial keratitis and hospitalized for tre...
To determine the transcriptional responses of human corneal epithelial cells (HCECs) after herpes simplex virus type (HSV)-1 infection and to identify the critical inflammatory element(s).
Immortalized HCECs were infected with HSV-1, and the global transcriptional profile determined. Molecular signaling networks were constructed from the HSV-1-indu...
To evaluate the correlation between changes in the susceptibility of bacteria and mutations in the quinolone-resistance determining region (QRDR) after 3 weeks of continuous fluoroquinolone instillation.
Miyata Eye Hospital, Miyazaki, Japan.
In this prospective randomized study, gatifloxacin 0.3% eyedrops or levofloxacin 0.5% eyedrops were administ...
To identify the candidate genes for pterygia recurrence from a pterygia transcriptome and to analyze their transcriptional regulation and functional relationships.
Transcriptional networks for pterygia recurrence were constructed using network analysis that was applied to 184 genes that showed a significant twofold change in the whole genome. Of th...
To characterize the roles played by monocyte chemoattractant protein-1 and its preferential receptor CCR2 (MCP-1/CCL2) in acute allergic inflammation.
The direct effects of MCP-1 were evaluated histologically after a subconjunctival injection of recombinant MCP-1 into naïve mice. The mice were sensitized to ragweed pollen, and allergic conjunctivit...
To determine how CpG, an immunostimulatory sequence, affects experimental allergic conjunctivitis and to determine the mechanisms of its action.
Experimental allergic conjunctivitis was induced in mice to investigate the suppressive mechanism of CpG treatment. Cytokine profiling, fluorescence-activated cell sorting analyses, and adoptive transfer w...
To study variations of Epstein-Barr virus (EBV), we analyzed the gp350/220 gene for several cell lines and Japanese wild isolates using direct sequencing. The N-terminal region was highly conserved in all EBVs except for Jijoye/P3HR-1 and a few isolates. The variation of the region coincided with EBV types A and B (also referred to as types 1 and 2...
The paper reports on Candida albicans ocular infection modified by steroid eye drops. A 74-year-old male complained of conjunctival injection and pain in his right eye three months after pterygium and cataract surgery. After treatment with antibiotics and steroid eye drops for three days, he was referred to our hospital. Clear localized corneal end...
To characterize the transcriptome of allergic conjunctivitis mediated by eosinophil-related chemokine receptor CCR3 and to identify a candidate for possible therapeutic intervention in eosinophilic inflammation of the eye.
Mice were sensitized to ragweed pollen, and allergic conjunctivitis was induced by an allergen challenge. The induction of alle...
Clinical findings and treatment of keratomycosis caused by Beauveria bassiana, an entomopathogenic filamentous fungus, are described for an 80-year-old woman, who was referred to the hospital for ocular pain and redness on the 9th day after an ocular injury caused by the frame of her glasses. She had a long history of recurrent diabetic iritis and...
To survey the use of silicone oil in clinical ophthalmology in Japan.
Questionnaires were sent to 1,240 hospitals registered as being ophthalmology residency training institutions with the Japanese Ophthalmological Society as of September 2007. Responses were collected via the Internet and results totaled. The use of silicone oil at each institutio...
Herpetic stromal keratitis (HSK) is an immunopathological reaction to herpes simplex virus type 1 (HSV-1) corneal infection. It has been reported that CD4+ cells play the most important role in the pathogenesis of this disease. In this study, we have focused on two chemokine receptors, CCR5 and CXCR3, which are expressed on CD4+ Th1 cells in mice H...
Herpetic stromal keratitis (HSK) is an immune reaction related to herpes simplex virus (HSV) corneal infection, and has many important immunological aspects. CD4(+) T lymphocytes, especially Th1 cells, are the principal mediators for HSK. In addition, neutrophils and antigen-presenting cells play vital roles in HSK. CD8(+) T lymphocytes, B cells, a...
To investigate the therapeutic effects of topical tacrolimus ointment on refractory ocular surface inflammatory diseases.
Retrospective interventional consecutive case series.
Ten consecutive patients with severe ocular surface inflammatory diseases who were suspected to be steroid responders (elevation of intraocular pressure [IOP]) or were refrac...
Citations
... Conversely, Rajneesh et al. found myopia to be more common, suggesting that the spectrum of visual impairments associated with VKC can vary significantly across populations. Keratoconus was the second most common complication observed (26.2%), closely aligning with the prevalence reported by Wajnsztajn D et al., highlighting the critical need for timely intervention, such as collagen cross-linking, to prevent severe visual impairment and the necessity for keratoplasty (2,15). The incidence of steroid-induced glaucoma and cataract in our study (4.8% each) reflects the risks associated with unmonitored steroid use, a concern similarly reported by Al-Akily SA et al (16). ...
... However, when innervation is impaired for any reason (iatrogenic injury, diabetes, or other causes), then wound healing may be delayed, leading to chronic ulceration. To accelerate wound healing, clinicians often prescribe topical antibiotics, such as levofloxacin, in combination with topical corticosteroids [96]. Incomplete reinnervation can lead to a decline in corneal sensation, which, while fortunately rare, can be a cause of morbidity in some patients undergoing cornea procedures. ...
... -β, VEGF, and CCL2 using a commercial ELISA kit (Quansys Biosciences; West Logan, UT). In addition, the PeproTech (Rocky Hill, NJ) was used for CCL2, and the R&D Systems (Minneapolis, MN) for VEGF.9,39,45 ...
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... A prospective observational study conducted in Japan revealed that 52 of 94 (55.3%) isolated fungi were filamentous fungi, and Fusarium spp. were the most frequently detected species (23 isolates) [3]. These findings indicate that Fusarium spp. ...
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... In this context the study of ocular isolates is relevant because some fungi that show emerging resistance, such as Candida glabrata and Candida auris, are currently rare corneal pathogens [47]. Although there are relatively few studies of corneal isolates [32,39,50,51], an increase in resistance for natamycin and voriconazole against Fusarium spp and Aspergillus spp has been reported [52]. We could find no published evidence for a difference in the susceptibility profile of common corneal isolates from the UK compared to isolates from other regions. ...
... The postoperative stability of the IOL position is the critical fundament of achieving optimal visual outcomes, with the match between the capsular bag and IOL serving as the determinant (2,3,5). However, whether the regular biometry could predict the LD has not yet been reached, and the best approach to measure or calculate the LD was also sought for (14)(15)(16). In this study, we used a novel UBM to measure LD and investigate the relationships between the LD and ocular biometric parameters, and observed that a larger LD was associated with larger WTW, ASL and AL in eyes without extreme myopia, while ASL was the only significant predictor in eyes with extreme myopia. ...
... Распространенность и многообразие патологий глазной поверхности, минимальные клинические изменения в начальных стади-ях ряда заболеваний, индивидуальные анатомические особенности капиллярной сети предъявляют высокие требования к квалификации врача-офтальмолога, проводящего осмотр. В последние годы развивается новое направление диагностики, связанное с компьютерной обработкой цифровых снимков глаза, полученных с помощью специализированного офтальмологического оборудования (цифровых фотощелевых ламп), основанной на методах машинного обучения [13][14][15][16][17]. ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/278585165271040-1443431287119_Q64/Yuji-Ayatsuka-2.jpg)
... As shown in Fig 9B, the ligand-receptor pairs with the highest prioritization score are Tnf, expressed by iMOs and neutrophils, interacting with BECs. This may be involved in inducing the expression of downstream targets involved in angiogenesis, both positive (Irf7, Plau) and negative (Gbp2, Lif) regulation, positive regulation of coagulation (Plau, Plaur) and in inflammation, with also both pro-(Icam1, Irf7, Nfkb2, Nfkbia, Tap1) and anti-inflammatory signals (Bcl2a1b, Tnfaip2, Tnfaip3, Tnip1) [29, [59][60][61][62][63][64][65][66][67][68][69]. At d12, AMs, Macros and fibroblasts express insulin-like growth factor (Igf1) and mesothelial cells express fibroblast growth factor 1 (Fgf1) which both promote angiogenesis (Fig 9A, right panel). ...
... After disruption of the mitochondrial structure, mitochondrial DNA (mtDNA) leakage into the cytoplasm can occur. As fully unmethylated DNA, the leaked mtDNA probably leads to TLR9 activation, which in turn activates downstream in ammatory pathways, causes the release of TNFα and IL-1β, and ultimately results in lung injury [8][9][10]. Mitophagy, as an important mechanism of mitochondrial quality control [11,12], can remove damaged mitochondria, reduce the leakage of mtDNA, and maintain stability of the body through three pathways, namely the PINK1/Parkin, BNIP3/NIX, and FUNDC1 pathways. ...
... With regards to the overall clinical severity of AU and viral load, the highest of both is typically in VZV-AU, followed by HSV-AU and lastly, CMV-AU. Iris atrophy is often observed in HSV-AU and VZV-AU typically with round type and sector type morphology, respectively [96][97][98]. ...
