Prashant Garg

Prashant Garg
L V Prasad Eye Institute | LVPEI · Segment of Cornea & Anterior (Vijayawada)

About

224
Publications
30,272
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5,657
Citations
Citations since 2016
98 Research Items
3182 Citations
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20162017201820192020202120220200400600
20162017201820192020202120220200400600
20162017201820192020202120220200400600

Publications

Publications (224)
Article
Full-text available
Background: To examine the relationship between single-nucleotide polymorphisms (SNPs) in interleukin (IL) genes and keratitis and its clinical manifestations. Methods: SNPs in IL1B, IL6, CXCL8, IL10, and IL12B were analysed. Differences in frequencies of alleles, genotypes and haplotypes between cases and controls as well as associations betwee...
Article
Toxic anterior segment syndrome (TASS) is an inflammation that occurs after anterior segment surgeries, and is widely seen in the patients after cataract surgery. The main cause of TASS is postulated to be the bio-residue and heat stable endotoxins which persist on the used forceps even after autoclave sterilization, some of which are known to caus...
Article
Biofilms are a critical health concern because of their ability to render tolerance and resistance against antimicrobials, which is especially challenging for surgical-site sterility and surgical wound recovery. Development of biofilm-inhibiting coatings on surgical devices could aid to limit biofilm formation at surgical site. In this context, two...
Article
Purpose: The aims of this study were to assess the trends in microorganisms from patients with infectious keratitis and to assess their antibiogram patterns at a tertiary eye care center in India. Methods: In this retrospective observational case series, microbiological records of all corneal ulcers were reviewed from 1991 to 2020 and assessed f...
Article
The field of ophthalmic drug delivery is undergoing rapid changes not only in the evolution of pharmacologic agents but also in the novel drug delivery vehicles. The ocular surface has limitations to drug penetration because of the presence of tight junctions between basal epithelial cells, which limits the amount of drug that can be absorbed after...
Article
Full-text available
The penetration of large molecular weight and poorly aqueous soluble drugs within the cornea has been a limiting factor in the treatment of ocular conditions. The objective of the present study was to fabricate rapidly dissolving polymeric microneedle ocular patch (MOP) to effectively deliver cyclosporine A (CsA) within the cornea. Microneedles wer...
Article
Purpose: To study the changing trends in the morphology of cataracts at a tertiary eye care center in South India due to COVID-19 pandemic-related national lockdown. Methods: A retrospective study conducted at a tertiary eye care center in Andhra Pradesh state of South India, which included 1724 patients (1753 eyes) who underwent cataract surg...
Article
Corneal ulcers, a leading cause of blindness in the developing world are treated inappropriately without prior microbiology assessment because of issues related to availability or cost of accessing these services. In this work we aimed to develop a device for identifying the presence of Gram-positive or Gram-negative bacteria or fungi that can be u...
Article
The delivery of therapeutics to the posterior segment of the eye is achieved by invasive procedures, including intravitreal injections and implants. The topically applied formulations would not permeate through different tissue barriers of the eye to reach the posterior segment. Here, we demonstrate the effectiveness of microneedle scleral patch in...
Article
Purpose: The purpose of this study was to evaluate the prevalence of SARS-CoV-2 in human postmortem ocular tissues of asymptomatic donors and its implications on our eye banking protocols. Methods: The expression of SARS-CoV-2 RNA was assessed by reverse transcription-polymerase chain reaction in corneal rims and conjunctival tissues from 100 do...
Presentation
Full-text available
The purpose of the present study was to fabricate a rapidly dissolving polymeric microneedle ocular patch (MOP) to effectively deliver large molecular weight and poorly aqueous soluble drug, cyclosporine A within the cornea.
Article
Full-text available
Purpose: To study impact of COVID-19 related national lockdown on care of corneal transplantation patients at a tertiary eye centre in Andhra Pradesh state of South India. Methods: Cross-sectional questionnaire based study conducted at tertiary eye care centre in Andhra Pradesh state of South India, included 109 patients who underwent keratoplasty(...
Article
Purpose: The purpose of this study was to report 3 cases of microbial keratitis, wherein trypan blue staining was used to aid the microbiological diagnosis of fungal keratitis and Pythium keratitis in a rural setup. Methods: Three consecutive patients who presented with a diagnosis of infectious keratitis underwent corneal scraping, and the smea...
Article
Full-text available
Branched poly( N - iso propylacrylamide) was functionalized with Amphotericin B (AmB) at the chain ends to produce an antifungal material. The polymer showed antifungal properties against AmB-sensitive strains of Candida albicans , Fusarium keratoplasticum and Aspergillus flavus (minimal inhibitory concentration ranged from 5 to 500 µg ml ⁻¹ ) but...
Article
Full-text available
Purpose: To evaluate the efficacy of a nanosilver-based sol-gel coating in preventing biofilm formation on contact lens cases. Methods: An organic-inorganic hybrid silica-zirconia sol formulation with immobilized silver nanoparticles was deposited on contact lens case coupons. The coated and uncoated coupons were subjected to biofilm formation t...
Chapter
Microbial keratitis is a significant cause of corneal blindness and ocular morbidity in the developing world. The problem is compounded by easy access to over-the-counter medications, delay in presentation to eye care facility, and irrational management. It is crucial to systematically work up a case of microbial keratitis so as to assess the sever...
Article
We report three cases of bilateral microsporidial keratitis, which is an unusual presentation. All three patients presented with bilateral, simultaneous, asymmetrical, deep stromal corneal infiltrates with symptoms ranging from 5 to 12 months. Predisposing factors were noted in two of three patients. Corneal scrapings for microbiology and histopath...
Article
Laser refractive surgery (LRS) is one of the most demanding areas of ophthalmic surgery and high level of precision is required to meet outcome expectations of patients. Post-operative recovery is of vital importance. Keratitis occurring after LRS can delay visual recovery. Both surface ablations [Photorefractive keratectomy (PRK)] as well as flap...
Article
Microbial keratitis is an infectious disease of the eye, in which the cornea is inflamed. Under severe conditions, keratitis can lead to significant loss of vision and enucleation of the eye. Ocular trauma is the major risk factor causing keratitis and microorganisms viz., bacteria, fungi, viruses are the causative agents. The current study charact...
Article
Full-text available
Bacterial keratitis is a corneal infection which may cause visual impairment or even loss of the infected eye. It remains a major cause of blindness in the developing world. Staphylococcus aureus and Pseudomonas aeruginosa are common causative agents and these bacterial species are known to colonise the corneal surface as biofilm populations. Biofi...
Article
Objectives: To identify risk factors associated with contact lens-related microbial keratitis (CL-MK) and subsequent vision loss in a south Indian population. Methods: A retrospective study of medical records at the LV Prasad Eye Institute in Hyderabad, India, of patients diagnosed with CL-MK and of controls who had no history of corneal inflamm...
Article
To compare the efficacy of topical voriconazole 1% and the combination therapy of 0.02% polyhexamethylene biguanide (PHMB) and 0.02% chlorhexidine for the treatment of Acanthamoeba keratitis (AK). This is a prospective, pilot, double-masked randomized comparative study. Twenty-three eyes of 23 patients with microbiologically (smear and/or growth on...
Article
Objective To investigate differences in SNPs in TLR genes between people who had keratitis and controls in an Indian population. Methods 145 cases of keratitis and 189 matched controls were recruited. DNA was extracted from peripheral blood. Single nucleotide polymorphisms (SNP) in TLR2 (n = 6), TLR4 (n = 15), TLR5 (n = 13) and TLR9 (n = 10) were...
Article
Full-text available
Dear Editor: Project ECHO (extension for community healthcare outcomes) facilitates upskilling of heath care workforce by interactive sharing of knowledge using an internet-based platform. This model originated at the University of New Mexico (UNM) Health Sciences Centre, USA in 2003, to address shortage of trained health care providers to treat he...
Article
Objective: The objective of the study was to design, fabricate, and investigate the effectiveness of microneedle ocular patch (MOP) in delivering the model drug, pilocarpine HCl across the corneal membrane. Methods: MOP mimicked commercially available contact lens design elements having a diameter of 14.20 mm and a sagittal height of 3.85 mm with a...
Article
When developing novel antimicrobials, the success of animal trials is dependent on accurate extrapolation of antimicrobial efficacy from in vitro tests to animal infections in vivo. The existing in vitro tests typically overestimate antimicrobial efficacy as the presence of host tissue as a diffusion barrier is not accounted for. To overcome this b...
Article
Full-text available
P. aeruginosa is the most common Gram-negative organism causing bacterial keratitis. Pseudomonas utilizes various virulence mechanisms to adhere and colonize in the host tissue. In the present study, we examined virulence factors associated with thirty-four clinical P. aeruginosa isolates collected from keratitis patients seeking care at L V Prasad...
Article
Full-text available
Purpose: To report and analyze the outcomes of therapeutic deep anterior lamellar keratoplasty (DALK) in patients with advanced Acanthamoeba keratitis (AK). Methods: Medical records of microbiologically confirmed AK, underwent DALK from 2004 to 2017, were reviewed and the data related to early and late outcome including complications were retrie...
Article
Full-text available
Cyclam-based antibacterial molecules (CAMs) that displayed potent activity against both planktonic and stationary phase of multidrug-resistant Gram-negative bacteria were rationally designed. The optimized compound retained its activity in human plasma and eradicated prefored biofilms. It also revealed excellent potency in ex-vivo model of human co...
Article
Full-text available
Purpose The global survey of eye banking and corneal transplantation reveals differences in eye banking trajectories in various countries. There is a need to encourage and lay down foundations of successful eye banking practices in many nations across the world map. The study evaluates demographics, trends in donor cornea retrieval, utilization and...
Article
Introduction: Corneal ulceration is one of the leading causes of blindness especially in low- and mid-income countries (LMICs). Surgical treatment of microbial keratitis is associated with multiple challenges that include non–availability of donor corneal tissues, lack of trained corneal surgeons, and poor compliance to follow up care. As a result,...
Article
Topical application of poorly water-soluble antibiotics cannot achieve the desired therapeutic concentration within cornea. The purpose of this study was to fabricate, characterize and evaluate in-vivo effectiveness of amphotericin B (AmB) containing microneedle ocular patch (MOP) against fungal keratitis. MOP containing free or liposomal AmB was f...
Article
Full-text available
Keratitis, an inflammatory disease of the eye, when neglected could lead to sight-threatening complications and ultimately blindness. Globally, over a million people are affected by keratitis annually. Keratitis has a microbial etiology and is caused by bacteria, fungi, viruses, etc. The present study compared the ocular surface fungal microbiome o...
Article
Full-text available
Purpose: To study the trends in collection, storage and utilization of donor corneas in eye banks in India. Methods: The data was collected from 12 eye banks in India that collected more than 1000 corneas per year. The retrospective analysis of the parameters like characteristics of the donor and the host, storage media used, number of eyes coll...
Article
Purpose: To report the outcomes of corneal transplantation using donor corneas retrieved from snakebite victims. Methods: This study was a retrospective analysis of 30 corneal transplants performed from 2014 to 2016 using donor corneas retrieved from snakebite victims. The details of the donor corneal characteristics and the transplant recipient...
Article
Full-text available
Streptococcus pneumoniae is the leading cause of bacterial keratitis in the developing world with a growing trend of acquiring resistance against various antibiotics. In the current study, we determined the expression of different antimicrobial peptides (AMPs) in response to S. pneumoniae in patients, as well as in primary and immortalized human co...
Article
We report a rare presentation of herpetic stromal keratitis reactivation in a 43-yearold patient who underwent LASIK previously and presented with complaints of photophobia, pain, and redness. On examination, mild congestion was noted with deep-seated, ring-like infiltrate under the LASIK flap with some amount of scarring that was initially confuse...
Article
Introduction: Fungal keratitis is an important cause of corneal opacity in developing nations. The medical management poses several challenges that include non-availability of drugs, lack of ophthalmic formulations of several potent drugs; need for prolonged treatment. It is not uncommon to face treatment failure. Therefore, surgery plays an import...
Article
Full-text available
Dysbiosis, or imbalance in the gut microbiome, has been implicated in auto-immune, inflammatory, neurological diseases as well as in cancers. More recently it has also been shown to be associated with ocular diseases. In the present study, the association of gut microbiome dysbiosis with bacterial Keratitis, an inflammatory eye disease which signif...
Article
Full-text available
Purpose: The aim of the study was to develop a novel formulation of levofloxacin and besifloxacin to achieve improved mucoadhesion and permeability of besifloxacin and levofloxacin through cornea for the effective treatment of ocular infections. Methods: A multicomponent hydrogel formulation containing chitosan-polyvinyl alcohol (PVA)-poly(N-vin...
Article
Full-text available
Macular corneal dystrophy is autosomal recessive dystrophy characterized by deposits of abnormal glycosaminoglycans in stromal lamellae and within endothelial cells. Deep anterior lamellar keratoplasty is successful in the management of this dystrophy. We herein describe three cases of primary graft failure after uneventful big bubble deep anterior...
Article
Purpose: To survey the demographics, risk factors, microbiology and outcomes for infectious keratitis in Asia. Design: Prospective, non-randomized clinical study METHODS: Thirteen study centers and 30 sub-centers recruited consecutive subjects over 12 - 18 months, and performed standardized data collection. A microbiological protocol standardize...
Article
Full-text available
Dysbiosis in the gut microbiome has been implicated in several diseases including auto-immune diseases, inflammatory diseases, cancers and mental disorders. Keratitis is an inflammatory disease of the eye significantly contributing to corneal blindness in the developing world. It would be worthwhile to investigate the possibility of dysbiosis in th...
Data
Core OTUs of the fungal microbiome libraries (OTUs having ≥ 0.001% abundance in a sample and ubiquitously present in over 80% of the samples). (DOC)
Data
Relative abundances of bacterial phyla in the studied cohort. Only those phyla having mean abundance > 1% are included in the table. (XLSX)
Data
Discriminating OTUs identified through pairwise Wilcoxon test between three groups of samples (Healthy Controls (HC) vs. Fungal Keratitis_Treated (FK_T), HC vs. Fungal Keratitis_UnTreated (FK_UT) and FK_T vs. FK_UT). (DOC)
Data
Relative abundances of KEGG pathways inferred from respective 16S taxonomic profiles of the studied cohort. (XLSX)
Data
Rarefaction curves of the fungal microbiome libraries of the 62 fecal samples from healthy controls and fungal Keratitis patients. (TIF)
Data
Details of healthy controls and fungal Keratitis patients. (DOC)
Data
Relative abundances of fungal OTUs for 62 fungal microbiomes. Sparse OTUs (with < 0.001% reads) were removed. (XLSX)
Data
Details of sequencing reads for 62 fungal microbiomes. (XLSX)
Data
Results of Wilcoxon test to identify discriminating fungal taxa. [results depicted at genera level; genera having median abundance > 0% in at least one class of samples (either FK or HC) included in table]. (DOC)
Data
Relative abundances of bacterial OTUs for 58 bacterial microbiome libraries. Sparse OTUs (with < 0.001% reads) were removed. (XLSX)
Data
Details of sequencing reads for 63 bacterial microbiomes. (XLSX)
Data
Relative abundances of bacterial families in the studied cohort. Only those families having mean abundance > 1% are included in the table. (XLSX)
Data
Core OTUs in the bacterial microbiome libraries of both HC and FK samples. (OTUs having ≥ 0.01% abundance in a sample and ubiquitously present in over 80% of the FK and HC samples). (DOC)
Data
Core OTUs (having ≥ 0.01% abundance in a sample and ubiquitously present in over 80% of the HC fecal samples) in the bacterial microbiome libraries of HC samples. (DOCX)
Data
Summary of KEGG pathways observed in the studied cohort. The pathways that showed significantly different enrichment in each dataset were identified using Wilcoxon test (BH corrected P-value < 0.05 was considered as significant). (XLSX)
Data
Taxonomic abundances of different bacterial families, across HC and FK samples. Only those families with > 1% mean abundance are depicted in the plot. (TIF)
Data
Relative abundances of fungal phyla in the studied cohort. (XLSX)
Data
Core OTUs (having ≥ 0.01% abundance in a sample and ubiquitously present in over 80% of the FK fecal samples) in the bacterial microbiome libraries of FK samples. (DOC)
Data
Relative abundances of KEGG modules inferred from respective 16S taxonomic profiles of the studied cohort. (XLSX)
Data
Summary of KEGG functional modules observed in the studied cohort. The modules that showed significantly different enrichment in each dataset were identified using Wilcoxon test (BH corrected P-value < 0.05 was considered as significant). (XLSX)
Data