Yuyang Zeng's research while affiliated with Tongji Hospital and other places

... However, due to the large size of the fibers compared to the scale of cells, the scaffolds fail to provide a microenvironment conducive to cell adhesion [21]. Melt electrowritten (MEW) is a 3D printing technology that is gaining popularity due to its ability to create highly ordered structures with a high resolution [22,23]. This technology can be used to fabricate highly porous scaffolds with appropriate cell adherence, penetration, and proliferation to meet the specific requirements of various tissue regeneration [24][25][26]. ...

... 89 On the other hand, there is evidence that the formation of a new vascular network under the influence of SVF can not only be a consequence of a direct stimulating effect on existing cells (host cells), but also develop due to the so-called disassembly and reassembly of the vascular network. 90,91 It is known that adipose tissue contains a huge number of blood vessels, the destruction of which occurs during the preparation of SVF (disassembly of the vasculature). Then, when SVF is introduced into the damaged area, the disconnected cells of the vascular wall are able, together with the existing blood vessels of the host, to create a hybrid vascular network (assembly). ...

... The research outcomes indicate that ADSC-derived exosomal miR-146a efficiently attenuates the production of pro-inflammatory cytokines released by osteoclasts in response to elevated glucose concentrations, provokes the deactivation of inflammasomes, impedes bone resorption, and ultimately ameliorates bone loss in diabetic OP rat models [97]. Empirical evidence demonstrates that ADSC-EVs can enhance the biocompatibility of titanium (Ti) medical implants [98]. Chen ingeniously assembled biotinylated MSC-EVs onto biotin-doped polypyrrole titanium (Bio-Ppy-Ti) surfaces, exhibiting superior cellular compatibility and osteoinductive properties in vitro compared to pure titanium, thereby presenting promising clinical applications [99]. ...

... For instance, Zeng et al. developed a controlled-release system based on a collagen-GO sponge loaded with alendronate sodium for the treatment of osteoporotic bone defects. This material prolonged the release of the drug, effectively inhibited the differentiation of monocyte-macrophages to osteoclasts, reduced bone loss in osteoporotic rats, and increased the volume of new bone at the defect site [80]. Similarly, Qin et al. prepared polyethylene glycol and polyethylene imide-functionalized GO nanocomplexes for the loading and delivery of miR-29b, which was involved in multiple steps of bone formation. ...

... 16,39 Moreover, a similar effect of ALN on various cell types was observed, when gradually released from the scaffolds, indicating successful scaffold functionalization without the loss of the ALN therapeutic effect. 16,20,[40][41][42] Although the release study showed the different release kinetics of ALN, the effect did not manifest during the coculture in vitro study. This could have been caused by the multiple exchange of the culture medium, which was necessary due to the needs of different media in different phases of the cell co-culture. ...

... In both studies, an excisional model of wounds was used, and both showed significantly better outcomes following NAC administration. Similarly, Hou et al. [21] have reported faster wound closure and better epidermal maturation with sustained NAC release using polycaprolactone/collagen scaffolds. The study also used an oval full-depth wound model, showing the potential benefits of long-term NAC administration using different administration vehicles, wound models and clinical contexts. ...

... Raman spectrum analysis was conducted to identify microscaffold components (Fig. 3C). Two peaks at 1354 cm −1 and 1598 cm −1 , corresponding to the D band and G band, indicated the successful incorporation of GO into the microscaffolds [35]. The intensity of the D and G band peaks was lower in the MLM GCH group due to interactions between GO and HAp that reduced lattice defects of the GO bands [36]. ...

... Empirical evidence demonstrates that ADSC-EVs can enhance the biocompatibility of titanium (Ti) medical implants [98]. Chen ingeniously assembled biotinylated MSC-EVs onto biotin-doped polypyrrole titanium (Bio-Ppy-Ti) surfaces, exhibiting superior cellular compatibility and osteoinductive properties in vitro compared to pure titanium, thereby presenting promising clinical applications [99]. Li et al reported the development and assessment of a novel acellular tissue-engineered bone construct, achieved by combining ADSC-Exos with poly (lactic-co-glycolic acid) (PLGA) scaffolds, significantly augmenting bone regeneration and offering a groundbreaking therapeutic paradigm for bone tissue engineering [100] ( Table 2). ...

... Various auxetic materials for biomedical applications. 239,[246][247][248][249][251][252][253][254][255] environment and tissue cell signaling, transcription factor activation, and the epigenome require further exploration. (3) Synergistic effects of mechanical and other physicochemical cues must be elucidated. ...

... Moreover, the loading of different therapeutic agents (natural and/or synthetic) in such structures can help in increasing the healing process and also help in the regeneration of the damaged tissue [21][22][23]. ...