Bernardetta Anna Tenuzzo's research while affiliated with Università del Salento and other places

... After the reaction finished, the suspension was cooled down at room temperature, and a dark brown solution was obtained. The resultant suspension was centrifuged using a cooling centrifuge at 10,000 rpm for 30 min under 10°C then the solution was purified in a dialysis bag for 48 h (retained molecular weight: (10,000-14,000 Da) (Mabrouk Zayed et al., 2022;Panzarini et al., 2022). The distilled water was changed every 24 h for the removal of impurities. ...

... Also, according to [39], phenolic compounds are able to inhibit proton motive force, electron transfer, oxidation of substrates, release of oxidative phosphorylation, inhibition of active transport, loss of metabolite balance, and disruption of the synthesis of DNA, RNA, lipid proteins, and polysaccharides. Phenolic compounds are macromolecular compounds that have many subgroups, including: flavonoids, phenolic acids, stilbenes, lignans, tannins, alkaloids, saponins, cardiac glycosides, sterols, and terpenoids [40]. ...

... On the other hand, the application of so-called pulsed electromagnetic fields (PEMF) has been empirically demonstrated to be an effective treatment for diseases such as arthritis [4][5][6][7][8][9], chronic pain [7,8,[10][11][12][13], bone injury [14][15][16][17][18][19][20][21], wound healing [22] and hyperinflammatory conditions [23,24]. This has resulted in publications on ELF-MF exposure of whole organisms as well as cells in culture, demonstrating effects on gene expression, cellular reactive oxygen concentration [25][26][27][28][29][30][31][32], membrane and lipid (raft) composition [33,34], ion channel activation [35] and literally dozens of other biological markers in differing cell types, organisms and plants [36]. A number of underlying mechanisms have been proposed for these effects, including the action of eddy currents induced by magnetic-field pulses [37]; ion cyclotron, stochastic [38,39] and paramagnetic resonance [40,41]; interference of quantum states of ions [42]; spin chemical effects on biochemical reaction intermediates [43][44][45]; magnetite nanoparticles [46]; and many more; see, e.g., discussion in [47]. ...

... These observations are in accordance with the previous study that documented the merits of ozonation over native oils [23]. The superior activity of OSO2% was probably due to the presence of a variety of ozone-catalyzed compounds [22,40] that are well-known for their diverse biological activities [45]. Furthermore, OSO2%, as compared to SO2%, strongly diminishes the CML-induced oxidative stress evident by the low fluorescent intensity in DHE-stained embryos. ...

... Sea urchins have been widely investigated since the XIX century due to the ease of collection, ecological and socioeconomic interest, high amount of available gametes from few individuals, external fertilization and synchronous embryo development (Agnello, 2017;Jangoux and Lawrence, 1983;Verlaque and Nédelec, 1983). They are traditionally important animal models for scientific research, particularly in the fields of ecotoxicology and developmental and reproductive physiology, which often result in significant applications in medical research (Agnello, 2017). ...

... Opsin2 and Opsin5 have been found only in echinoderms; therefore, they are thought to be specific to the group. Because some of the expression patterns of these genes have been reported in both embryos/larvae and adults [24][25][26][27], it is reasonably expected that sea urchins have the ability to react to light stimuli from a genomic perspective, as reported in adult behavioral studies [28,29]. However, the functions of photoreceptor genes have never been confirmed by using genetic modification except for the function of the recently reported gut-regulatory Go-Opsin [12], and the neural pathway regulating cilia-based larval behavior has not yet been identified. ...

... Our study is consistent with others, by demonstrating the sensitivity of breast cancer cells to treatment with AgNPs [26,27], especially on triple-negative breast cancer cells. This predisposition may be due to the long-chain polyunsaturated fatty acids enrichment characteristic in this type of cancer, which is prone to AgNPs-driven peroxidation [28]. ...

... Although the numerous publications about the E-NPs impact on the marine environment [7,[9][10][11][12][13][14][15][16][17][18][19][20][21][22], there is still a lack of knowledge in this area. ...

... Previous studies on sea urchins embryos from temperate regions conducted with different types of ENM/NPs have demonstrated deleterious effects not only on fertilization (Manzo et al., 2013;Gambardella et al., 2015;Maisano et al., 2015), but also on embryonic development (Manno et al., 2013;Della Torre et al., 2014;Pinsino et al., 2017;Pikula et al., 2020). On the other hand, the potential impact of ENM/NPs to echinoderms from different latitudes, such as those inhabiting tropical regions, is still poorly investigated. ...

... Moreover, the surface of nanocarriers can be coated by specific targeting molecules which may be hydrophilic or hydrophobic, or by molecules like aptamers, antibodies, peptides, or small compounds. On the site of diseased tissue, these drug-loaded molecules release the therapeutic agents in response to changes in the body's natural conditions such as pH level, temperature, osmolarity, and enzymatic activity (Panzarini et al., 2013). There is a widespread use of nanoparticle-based delivery systems in various medical fields as they can be easily customized so these tiny structures possess remarkable ability to precisely target specific cells. ...