... Various segmentation methodologies have been proposed to extract these quality parameters and keep visual differences based on textural heterogeneity [15,[35][36][37] and the morphological structure [10,20,27] of each cytological component (nuclei, cytoplasm, extracellular component, RBC, and background). These morphological and textural parameters are extracted with various segmentation approaches, e.g., thresholding technique [16], Otsu thresholding [10,28], Otsu thresholding followed by LDA [27], thresholding followed by k-means [17,20,24,25], k-means with graph cut method [30], textural parameter followed by PCA and k-means [18], L2E along with canny edge and Hough transformation [19,[38][39][40][41], gaussian mixer model and expectation maximization [9], mean shift elimination [21], contour models [21,23,[42][43][44][45][46][47][48][49], color-coded map based [29], watershed [37,50], piecewise [51] entropy-based histogram [31], level-set [52], and transfer learning approaches for feature extraction [32][33][34] have been introduced to segment histopathological images. Many works have been performed in image segmentation, available in survey resources. ...