Vincent Torre

Vincent Torre
Scuola Internazionale Superiore di Studi Avanzati di Trieste | SISSA · Area of Neuroscience

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334
Publications
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12,927
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Publications

Publications (334)
Article
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Seizures represent a frequent symptom in gliomas and significantly impact patient morbidity and quality of life. Although the pathogenesis of tumor-related seizures is not fully understood, accumulating evidence indicates a key role of the peritumoral microenvironment. Brain cancer cells interact with neurons by forming synapses with them and by re...
Article
Neural interfaces enable the monitoring of the state of the brain and its composite cell networks, as well as stimulate them to treat nervous disorders. In addition to their highly efficient charge transduction and stability during operation, the neural electrodes should avoid altering the physiological properties of targeted neuronal tissues. Two-...
Article
Full-text available
There is growing evidence suggesting that mechanical properties of central nervous system neurons may play an important regulatory role in cellular processes. Here we employ an Oscillatory Optical Tweezers (OOT) to exert a local indentation with forces in the range of 5 – 50 pN. We found that single local indentation above a threshold of 13 ± 1 pN...
Article
Full-text available
Cyclic nucleotide–gated (CNG) channels are key to the signal transduction machinery of certain sensory modalities both in vertebrate and invertebrate organisms. They translate a chemical change in cyclic nucleotide concentration into an electrical signal that can spread through sensory cells. Despite CNG and voltage-gated potassium channels sharin...
Article
Full-text available
Neural stem cell (NSC)-based therapy is a promising candidate for treating neurodegenerative diseases and the preclinical researches call an urgent need for regulating the growth and differentiation of such cells. The recognition that three-dimensional culture has the potential to be a biologically significant system has stimulated an extraordinary...
Article
The control of cell-microenvironment interactions plays a pivotal role in constructing specific scaffolds for tissue engineering. Here, we fabricated a 3D free-standing ordered graphene (3D-OG) network with precisely defined pattern. When primary cortical cells are cultured on 3D-OG scaffolds, they form well-defined 3D connections. Astrocytes have...
Article
Significance Rod photoreceptor outer segments (OSs) are specialized ciliary architectures. Our imaging experiments reveal three properties of rods. First, intracellular calcium and key proteins have a concentration varying from the OS base to the tip. At the OS base, [Ca2+] i is approximately 80 nM and increases up to ∼200 nM at the OS tip; second,...
Article
Full-text available
Photoreceptors are specialized cells devoted to the transduction of the incoming visual signals. Rods are able also to shed from their tip old disks and to synthesize at the base of the outer segment (OS) new disks. By combining electrophysiology, optical tweezers (OTs), and biochemistry, we investigate mechanosensitivity in the rods of Xenopus lae...
Article
Microglia are highly plastic cells that change their properties in response to their microenvironment. By using immunofluorescence, live-cell imaging, electrophysiological recordings and RNA sequencing, we investigated the regulation of modified bacterial cellulose (mBC) nanofibril substrates on microglial properties. We demonstrate that mBC substr...
Article
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The failure of existing therapies in treating human glioblastoma (GBM) mostly is due to the ability of GBM to infiltrate into healthy regions of the brain; however, the relationship between cell motility and cell mechanics is not well understood. Here, we used atomic force microscopy (AFM), live-cell imaging, and biochemical tools to study the conn...
Preprint
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The change in electrical property (capacitance) upon hybridization of the desired ssDNA to a capture probe has been proposed as a promising technology platform in biomedical research and practice. An appropriate mathematical model is needed for understanding and optimizing the process occurring at the electrode/electrolyte interface. It is also inf...
Article
Glioblastoma (GBM) is one of the most malignant brain tumours and, despite advances in treatment modalities, it remains largely incurable. Ca2+ regulation and dynamics play crucial roles in different aspects of cancer, but they have never been investigated in detail in GBM. Here, we report that spontaneous Ca2+ waves in GBM cells cause unusual intr...
Article
Full-text available
BACKGROUND: The spontaneous activity of neuronal networks has been studied in in vitro models such as brain slices and dissociated cultures. However, a comparison between their dynamical properties in these two types of biological samples is still missing and it would clarify the role of architecture in shaping networks’ operation. METHODS: We use...
Preprint
Full-text available
Is the mechanical unfolding of proteins just a technological feat applicable only to synthetic preparations or is it applicable to real biological samples? Here, we describe all the steps necessary to deal with native membranes, from the isolation of the membrane of single cells, to the characterization and identification of the embedded membrane p...
Article
Full-text available
In the article number 1806132, Guosheng Cheng, Francesco Paolo Ulloa Severino, Vincent Torre, and co‐workers develop a 3D graphene–CNT web which is fully geometrically, mechanically, and electrically interconnected. This monolithic scaffold allows the production of a functional 3D cortex‐like network for reconstructing the 3D trajectories and veloc...
Article
Full-text available
Several channels, ranging from TRP receptors to Gap junctions, allow the exchange of small organic solute across cell membrane. However, very little is known about the molecular mechanism of their permeation. Cyclic Nucleotide Gated (CNG) channels, despite their homology with K⁺ channels and in contrast with them, allow the passage of larger methyl...
Data
Hydration of the DMA in the CNG pore. Superimposition of 10 instantaneous configurations taken from MD simulations showing the DMA coordination in S3c. The black dashed line refers to H-bonds, while the red dashed circle indicates the hydrophobic interactions. The average number of water coordinating DMA is about 1.5. For simplicity only the residu...
Data
Evolution of the free energy profiles along time. Free energy profiles (kj/mol) as a function of the distance of the DMA from its binding site, represented by center of mass of Val64 (nm). Different colors refer to different times. (TIF)
Data
Currents activated by 1 mM cGMP for recombinant WT CNGA1 channel and pore mutants T359A and T360A in the presence of symmetrical DMA. (A) Sequence alignment in the selectivity filter of the NaK2CNG-E, bCNGA1, hCNGA1, hCNGA2 and hCNGA3 channels. In red, the Thr which have been mutated in Ala. (B) Structure of the NaK2CNG-E selectivity filter (PDB ID...
Data
Plumed files used in the BE-META scheme. Here are reported the Plumed files used to obtain the Free Energy profile reported in Fig 2. (PDF)
Data
A model for DMA permeation through the CNGA1 channels. A detailed explanation of the model reported in Fig 5. (PDF)
Data
DMA is shifted towards T63 in the presence of a second DMA. Right side: The tetrameric structure of a single DMA and 2 DMA complexes are superimposed and only the residues 62–68 are shown for the opposite subunits in the tetramer. The DMA complex is colored in yellow, while DMA-DMA complex in wheat. Changes in the position of the DMA can be observe...
Data
DMA permeation pathway in the DMA-DMA configuration. Free Energy profile (kJ/mol) of the DMA-DMA configuration along the distance of the DMA from its binding site, represented by center of mass of Val64 (nm). The free energy profile is shown with the relative error bars estimated by block analysis [29]. (TIF)
Article
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[This corrects the article on p. 130 in vol. 12, PMID: 29867363.].
Article
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Mechanical stresses are always present in the cellular environment and mechanotransduction occurs in all cells. Although many experimental approaches have been developed to investigate mechanotransduction, the physical properties of the mechanical stimulus have yet to be accurately characterized. Here, we propose a mechanical stimulation method emp...
Article
Full-text available
Tissue engineering strives to create functional components of organs with different cell types in vitro. One of the challenges is to fabricate scaffolds for three-dimensional (3D) cell culture under physiological conditions. Of particular interesting is to investigate the morphology and function of the central nervous system (CNS) cultured using su...
Article
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The folding dynamics of proteins at the single-molecule level has been studied with single-molecule force spectroscopy experiments for 20 years, but a common standardized method for the analysis of the collected data and for sharing among the scientific community members is still not available. We have developed a new open-source tool-Fodis-for the...
Article
Full-text available
Cyclic nucleotide-gated (CNG) channels mediate transduction in several sensory neurons. These channels use the free energy of CNs' binding to open the pore, a process referred to as gating. CNG channels belong to the superfamily of voltage-gated channels, where the motion of the α-helix S6 controls gating in most of its members. To date, only the o...
Article
Full-text available
A single-shot water-immersion digital holographic microscope combined with broadband (white light) illumination mode is presented. This double imaging platform allows conventional incoherent visualization with phase holographic imaging of inspected samples. The holographic architecture is implemented at the image space (that is, after passing the m...
Article
Full-text available
During neurite development, Actin Waves (AWs) emerge at the neurite base and move up to its tip, causing a transient retraction of the Growth Cone (GC). Many studies have shown that AWs are linked to outbursts of neurite growth and, therefore, contribute to the fast elongation of the nascent axon. Using long term live cell-imaging, we show that AWs...
Article
Full-text available
Nature Communications 8: Article number: 7093 (2015); Published: 12 May 2015; Updated: 29 September 2017 An error was inadvertently introduced into the fifth sentence of the Abstract in the original version of this Article. The sentence should have stated ‘Force spectra determined that the S4 transmembrane domain is mechanically coupled to S5 in th...
Article
Full-text available
By combining atomic force microscopy (AFM) imaging and single-molecule force spectroscopy (SMFS), we analyzed membrane proteins of the rod outer segments (OS). With this combined approach we were able to study the membrane proteins in their natural environment. In the plasma membrane we identified native cyclic nucleotide-gated (CNG) channels which...
Conference Paper
We present the combination of a single-shot water-immersion digital holographic microscopy with broadband illumination for simultaneous visualization of coherent and incoherent images using microbeads and different biosamples.
Conference Paper
Culture of primary neurons, and especially hippocampal neurons, is important for understanding cellular mechanisms in neurobiology. Actually, this is achieved by using culture dish or glass slide with surface coated proteins. Here, we proposed a patch method for culture of primary neurons on a monolayer of gelatin nanofibers electrospun and crossli...
Article
Full-text available
Local stimulation with optical tweezers has been used to mimic natural stimuli that occur in biological processes such as cell migration or differentiation. Carriers (beads and lipid vesicles) with sizes down to 30 nm can be manipulated with a high spatial and temporal resolution: they are positioned with a sub-micrometric precision on a specific c...
Conference Paper
Full-text available
We have analyzed the unfolding of membrane proteins from membranes of hippocampal and dorsal root ganglia (DRG) neurons. We removed the upper cell membrane of dissociated neurons from the hippocampus and DRG of juvenile P3 rats to perform SMFS from the cytoplasmic side of the membrane. We found several dozens of F-D traces with the saw-tooth like s...
Article
Background: Since different culturing parameters - such as media composition or cell density - lead to different experimental results, it is important to define the protocol used for neuronal cultures. The vital role of astrocytes in maintaining homeostasis of neurons - both in vivo and in vitro - is well established: the majority of improved cult...
Article
We fabricated a nanocomposite substrate made of nanofibers on micropillar arrays by photolithography, soft lithography and electrospinning for cell culture studies. This nanocomposite substrate combines the advantage of the physiological extra-cellular matrix (ECM)-like surface morphology and high porosity and low stiffness of the underneath suppor...
Article
Culture of primary neurons, and especially hippocampal neurons, is important for understanding cellular mechanisms in neurobiology. Actually, this is achieved by using culture dish or glass slide with surface coated proteins. Here, we proposed a patch method for culture of primary neurons on a monolayer of gelatin nanofibers electrospun and crossli...
Article
Full-text available
The determination at atomic resolution of the three-dimensional molecular structure of membrane proteins such as receptors and several ion channels has been a major breakthrough in structural biology. The molecular structure of several members of the superfamily of voltage-gated ionic channels such as K⁺ and Na⁺ is now available. However, despite s...
Poster
Full-text available
The actin cytoskeleton is a highly dynamic system that is dependent on the assembly and disassembly of filamentous actin (F-actin) from its monomeric form (G-actin) that undergoes a constant and rapid reshaping of actin polymer network. Different types of wave phenomena have been observed in the actin system of motile cells: in neuronal cells at th...
Article
Full-text available
Recent results from network theory show that complexity affects several dynamical properties of networks that favor synchronization. Here we show that synchronization in 2D and 3D neuronal networks is significantly different. Using dissociated hippocampal neurons we compared properties of cultures grown on a flat 2D substrates with those formed on...
Article
Human induced pluripotent stem cells (hiPSCs) are differentiated toward mature motor neurons by using a culture patch made of crosslinked monolayer gelatin nanofibers. Comparing to the conventional culture dish method, the patch method is more effective for culture and differentiation of stem cells, because cells are supported by a net-like structu...
Article
Full-text available
We used optical tweezers, video imaging, immunocytochemistry and a variety of inhibitors to analyze the role of Rac1 in the motility and force generation of lamellipodia and filopodia from developing growth cones of isolated Dorsal Root Ganglia neurons. When the activity of Rac1 was inhibited by the drug EHop-016, the period of lamellipodia protrus...
Article
Full-text available
Small RhoGTPases, such as Cdc42 and RhoA, are key players in integrating external cues and intracellular signaling pathways that regulate growth cone (GC) motility. Indeed, Cdc42 is involved in actin polymerization and filopodia formation, whereas RhoA induces GC collapse and neurite retraction through actomyosin contraction. In this study we emplo...