There is a bone mineral density (BMD) mean/range for the rat tibia? There is difference in the BMD values between male and female rats?

Any simple numerical example if any one you have 

Our lab looks at tumor growth in the bone microenvironment, and in doing so, I section mouse tibia on a fairly regular basis. These tibia have been manually cleaned, fixed in z-fix for a total of 48h (24h at RT, change z-fix, 24h at 4 degrees), then decalcified (10% EDTA, pH 6.5) for a total of 2 weeks at 4 degrees (changed after 1 week). They were then rinsed well with deionized water, put into 70% ethanol, and sent to Histoserv for paraffin embedding and initial sectioning for H&E sections. I then section these blocks for addition staining, and most of the time, everything is okay. However, I come across some blocks where, for some reason, part of the bone will chip out during the sectioning (as the blade goes through the block, you can actually see the chip of bone come out). I have tried to re-paraffin these blocks by softening them, placing the chip back in place, putting a small layer of paraffin on top using a spatula, then re-molding the block. Sometimes this works, sometimes this doesn't. Does anyone have any suggestions for what to do when the bone chips out of the block? Thank you!

Hello all, I have processed rabbit femurs and tibia however with this last batch a few bones were clearly undecalcified and so I have been unable to obtain full sections on the microtome. I have read about soaking blocks in a decalcifier (surface decal) but the sections still weren't being fully incorporated in the paraffin ribbon. Is it possible to reprocess and further decalcify the tissue? Also any suggestions of useful determinants for the endpoint of decalcification besides the use of CT? Thanks in advance!

I have a set of CT scans performed on a GE Revolution of bones of which I need to know Bone Mineral Density in gr/cm2. All I have is Hounsfield Units. Is there a way (software or similar) to obtain this conversion?

Hello,

I have been trying to do a rat bone Immunofluorescence histology study. But not able to find a successful protocol till now, Any suggestions?

Rat bone: Decalcifies

Embedding: Cryo

Hello everyone,

I've made a model in Abaqus which is 2 parts of tibia bone, callus and external fixator. I've used tet mesh and global size 2 for all parts; but I want to user a finer mesh for callus part. When I change the size from 2 to 1 the job is aborted and give me this type of error:

"The volume of 1 elements is zero, small, or negative. Check coordinates or node numbering, or modify the mesh seed. In the case of a tetrahedron this error may indicated that all nodes are located very nearly in a plane. The elements have been identified in element set ErrElemVolSmallNegZero."

Please help me!

What is the diameter of vessels in the bone?For example, tibia. Many thanks.

There exist multiple publicly available medical imaging datasets such as NSCLC Radiogenomics for lung cancer but I could not find any dataset of HR-pQCT of tibia from healthy adults. Does anyone know if access to such dataset exists?