Federal University of Santa Catarina
  • Florianópolis, Santa Catarina, Brazil
Recent publications
We describe Markov interval maps via branching systems and develop the theory of relative branching systems, characterizing when the associated representations of relative graph C*-algebras are faithful. When the Markov interval maps f have escape sets, we use our results to characterize injectivity of the associated relative graph algebra representations, improving on previous work by the first, third, and fourth authors.
A simple sample preparation approach employing the dispersive pipette extraction (DPX) technique is proposed to determine twelve polyphenols, including phenolic acids and flavonoids in wines, followed by identification and quantification by LC-MS/MS. The extraction parameters, including sample volume and pH, salting out effect, time and cycles of extraction and desorption, and desorption solvent were optimized using univariate and multivariate designs. The analytical performance was satisfactory, with determination coefficients greater than or equal to 0.9877, precisions with values lower than 20 %, and recoveries ranging from 87 to 114 %. The applicability of the method was evaluated in red wine. The major compounds determined in the sample were (–)-epicatechin (23.5 mg L–1), (+)-catechin (19.2 mg L–1), and myricetin (14.6 mg L–1). The green character of the analytical procedure and the sample preparation step were evaluated by three analytical metrics.
Cumulus cells from cumulus-oocyte complexes (COC) matured in vitro in serum-free medium show high incidence of apoptosis and DNA double-strand breaks (DSB). This study aimed to characterize the transcript expression profile of selected genes involved in DNA repair mechanisms in bovine cumulus cells cultured with bovine serum albumin (BSA) or fetal calf serum (FCS). Briefly, bovine cumulus-oocyte complexes were in vitro matured with either, 0.4% BSA or 10% FCS for 3, 6, 12 or 24 h. The total RNA of cumulus cells was used for real-time PCR analysis. Transcript abundance of XRCC6, XRCC5, DNAPK, GAAD45B, TP53BP1, RAD50, RAD52, ATM and BRCA2 target genes changed as the IVM proceeded (P < 0.05). However, an interaction between protein source (FCS or BSA) and time was not detected (P ≥ 0.05). Cumulus cells from COCs matured with BSA presented higher mRNA expression of two genes compared to FCS group: TP53BP1 at 6 h and BRCA1 at 3, 6, 12 and 24 h (P < 0.05). In summary, our results showed for the first time the expression profile of the key genes involved in DSB repair mechanisms in cumulus cells obtained from bovine COCs matured with FCS or BSA. The higher mRNA expression of BRCA1 and TP53BP1 and lower mRNA expression of TNFAIP6 suggests an increase in apoptosis rate and DNA damage in cumulus cells cultured in BSA-supplemented medium and may explain, at least to some extent, the reduced developmental potential of bovine oocytes matured in serum-free medium.
The transient receptor potential vanilloid type-1 (TRPV1) channels have been implicated in the modulation of aversive responses. The endocannabinoid anandamide acts as an endogenous TRPV1 agonist, exerting opposite functions at TRPV1 and type-1 cannabinoid receptors (CB1R). Here we tested the hypothesis that hippocampal TRPV1 modulates contextual fear memory retrieval and investigated the influence of the aversive stimulus intensity as well as the role of endocannabinoid signaling. Male C57BL/6J mice were tested for contextual fear memory after low-, moderate-, or high-intensity shock protocols. The selective TRPV1 blockers SB366791 (1-10 nmol) and 6-I-NC (2 nmol) were infused via intra-dorsal hippocampus before the retrieval test session. The local levels of endocannabinoids and Arc and Zif268 mRNAs, involved in synaptic plasticity and memory, were quantified. First, both TRPV1 blockers reduced memory retrieval in animals exposed to moderate or high (but not low) intensity training protocols. In the second series of results, the magnitude of the freezing responses positively correlated with the hippocampal anandamide levels; TRPV1 and CB1R were found co-localized in this brain region; and the CB1R antagonist, AM251, prevented the effects of SB366791. Thus, endocannabinoid signaling possibly mediates the effects of TRPV1 blockers. Finally, inhibition of memory retrieval by TRPV1 blockers increased Arc and Zif268 mRNAs and impaired fear memory reinstatement. In conclusion, the modulation of fear memories by dorsal hippocampal TRPV1 channels may depend on the aversive stimulus intensity and occur via anandamide/CB1 signaling. Moreover, TRPV1 blockers promote Arc and Zif268 transcription, with subsequent attenuation of aversive memory reinstatement.
The short-term generation scheduling (STGS) problem defines which units must operate and how much power they must deliver to satisfy the system demand over a planning horizon of up to two weeks. The problem is typically formulated as a large-scale mixed-integer linear programming problem, where off-the-shelf commercial solvers generally struggle to efficiently solve realistic instances of the STGS, mainly due to the large-scale of these models. Thus, decomposition approaches that break the model into smaller instances that are more easily handled are attractive alternatives to directly employing these solvers. This paper proposes a dual dynamic integer programming (DDiP) framework for solving the STGS problem efficiently. As in the standard DDiP approach, we use a nested Benders decomposition over the time horizon but introduce multiperiod stages and overlap strategies to accelerate the method. Simulations performed on the IEEE-118 system show that the proposed approach is significantly faster than standard DDiP and can deliver near-optimal solutions.
Although targeted magnetic hyperthermia has been proven to be an effective tumor ablation technique, its use in clinical applications is still scarce particularly due to the difficulty in imposing a desired nanofluid distribution in the therapeutic area. In addition to the inherent difficulty of imposing a distribution with few injection shots, during the nanofluid infusion, the tissue deformation can cause the nanofluid deviation from the targeted injection area and the backflow along the needle can deliver the injected nanofluid to the outer surface of the tissue. Both phenomena can result in an irregular distribution for nanofluid inside bio-tissue. This study develops a poroelastic model considering geometrically nonlinear behavior in order to evaluate the effect of syringe needle size and infusion rate on the backflow. A 26 gauge needle for syringe is used as a typical example to further investigate the nanofluid transport and the change of solid matrix material properties under different infusion rates after comparing the infusion results for several sizes of needle. Finally, the resulting nanofluid concentration distribution obtained with the proposed model is used to simulate the temperature distribution and the cancerous cell damage. The results demonstrate that the infusion pressure and its resulting tissue deformation are the fundamental reasons for obtaining an irregular solution distribution. Tissue deformation induces the increase of porosity and permeability for biomaterials around the tip, and enhances the fluidity of nanofluids inside the tissue. The results also indicate that the increase in backflow length can improve the uniformity of the nanofluid distribution after diffusion and, consequently, the treatment effect. However, it also increases the risk of MNP leakage from the targeted area to the tumor surface, so it is important to keep the backflow rate limited during the injection process.
Growing evidence from male rodent and human studies suggests that cannabidiol (CBD) modulates the expression of aversive memories and anxiety-related responses. The limited data on whether and how CBD influences these aspects in females could have therapeutic implications given the increased susceptibility of women to anxiety- and stress-related disorders relative to men. Female studies are also essential to examine inherent aspects that potentially contribute to differences in responsiveness to CBD. Here we addressed these questions in adult female rats. Contextually fear-conditioned animals acutely treated with CBD (1.0–10 mg/kg) were tested 45 min later. In subsequent experiments, we investigated the estrous cycle effects and the contribution of dorsal hippocampus (DH) serotonin 1A (5-HT1A) and cannabinoid types 1 (CB1) and 2 (CB2) receptors to CBD-induced effects on memory retrieval/expression. The effects of pre-retrieval systemic or intra-DH CBD administration on subsequent fear extinction were also assessed. Lastly, we evaluated the open arms avoidance and stretched-attend postures in females exposed to the elevated plus-maze after systemic CBD treatment. CBD 3.0 and 10 mg/kg administered before conditioned context exposure reduced females' freezing. This action remained unchanged across the estrous cycle and involved DH 5-HT1A receptors activation. Pre-retrieval CBD impaired memory reconsolidation and lowered fear during early extinction. CBD applied directly to the DH was sufficient to reproduce the effects of systemic CBD treatment. CBD 3.0 and 10 mg/kg reduced anxiety-related responses scored in the elevated plus-maze. Our findings demonstrate that CBD attenuates the behavioral manifestation of learned fear and anxiety in female rats.
A glassy carbon electrode was modified with nitrogen and sulfur co-doped graphene quantum dots immobilized in chitosan for the monitoring of multivitamins. The graphene quantum dots were synthesized using a simple citric acid/l-cysteine pyrolysis procedure. The co-doping with nitrogen and sulfur in the graphene matrix was confirmed by spectroscopic techniques. Electron microscopy results showed that the synthesized quantum dots had a diameter of 3.4 ± 1.4 nm. Electrochemical techniques showed excellent current responses to vitamin oxidation provided by the modified electrode compared to the bare electrode. The parameters of square wave voltammetry were optimized in order to obtain the best current responses and to study the electrochemical oxidation of vitamins. The calibration plots for vitamins B2, B6 and B12 were constructed in 0.1 mol L⁻¹ sodium acetate buffer (pH 5.0) with limits of detection of 0.30, 30.1 and 0.32 nmol L⁻¹, respectively. Lastly, the modified electrode was effectively implemented in the quantification of vitamins in classic and fruit-based energy drink samples.
This chapter describes the analysis of rectifier circuits valid for ultra-low-voltage (ULV) operation. Expressions for the output voltage, power conversion efficiency, and input resistance are presented for the Dickson charge pump and for the voltage multiplier. The expressions are derived for both square-wave and sine-wave signals and are expressed as a function of the diode parameters and the load current.KeywordsRectifierCharge pumpSwitched capacitorVoltage multiplierEnergy harvestingUltra low voltage
This chapter presents the design, implementation, and measurements of a DC-DC voltage converter for ultra-low-voltage energy-harvesting applications. The converter architecture is comprised of a cold starter, a high-efficiency inductive boost converter, and a control circuit. The cold starter, based on an ultra-low-voltage oscillator and a charge pump, provides the circuit startup for input voltages of down to 11 mV. The inductive boost converter was designed to achieve high-efficiency through the adoption of a nonlinear zero-current-switching scheme that minimizes the synchronization losses. The prototype provides steady-state operation for input voltages in the range of 7.3–140 mV with end-to-end efficiencies higher than 50% for input voltages above 10.5 mV and peak end-to-end efficiency of 85% at 140 mV.
Geogebra® is open-source software with a graphical interface directed to math and calculus teaching. Besides that, it is frequent to figure out teaching applications in geometry. This paper aims to demonstrate two applications of 3D dynamic models developed in Geogebra® directed to descriptive geometry online teaching. Specifically, the applications regard concepts that students struggle to acquire the visual skills to comprehend: the inclusion of lines in planes and the changing of projection planes. To evaluate the quality of the 3D models, we applied a quantitative-qualitative survey to descriptive geometry professors in Brazilian undergraduate courses, in which they could interact with the 3D models. The results indicated that the developed 3D dynamic models are adequate for online teaching applications. Additionally, they contain the essential features to support the visual skills teaching-learning process required in architecture and engineering courses. Finally, some limitations and suggestions of improvement were pointed out by the participants. KeywordsGeoGebraDescriptive geometry3D dynamic models
The use of models generated by 3D scanning in Design projects, particularly in the production of packaging, has been gaining relevance as scanning systems become popular. However, the high costs of these devices are an obstacle to their large-scale use. This article is incorporated into the proposal of a low-cost method, by photogrammetry using smartphones and open source software, aimed to 3D scanning of fresh foods. Experiments presented allow the comparison among results provided by different camera resolutions and variations in textures, colors and food morphology. Three scenarios for capturing images are evaluated, demonstrating that the simplest of them, with a fixed base, produces good results, faster than those performed with a rotating base or a mini studio. Some structural packaging projects are carried out from the images generated by the use of the proposed low-cost method.
A numerical-experimental methodology is presented in this study to estimate the absorption efficiency in a laser welding process by estimating the rate of energy transferred to a metal plate. The iterative Function Specification Method was modified to account for moving temperature sensor thermal sensitivity as a function of time and position relative to the welding bead. Thus, highly nonlinear problems can be solved by using a high-temperature gradient in the measurement sensor region. Three experiments on an AISI 1020 steel sheet were carried out using a 3 kW fiber laser and a 3 m/min welding speed. A thermo-fluid model was used with solid–liquid phase changes, buoyancy forces, and the Marangoni effect in the welding pool to model the physical phenomena. A code in Matlab was developed to solve the inverse problem. The direct problem was solved using COMSOL Multiphysics through the Livelink for Matlab feature. The average absorption efficiency was 79.5% for the welding process. A comparison was made between the geometry of the welding bead obtained in experiments with the numerically calculated welding bead to validate the model. The results obtained in this article are intended to assist simulations in laser welding processes and are in agreement with the literature data.
Organic wastes, fertilizers, organominerals, and minerals contain nutrients essential to crops and contribute to increase productivity. However their use can also lead to the accumulation of copper and zinc, which changes the distribution of their fractions and enhances the risk of toxicity to plants and the environment, thereby working against the sustainable development goals. However, the effect of manure applications on clayey soils and the effects of increasing Cu and Zn contents over the years on crop productivity remain unknown. This study aimed to evaluate the distribution and the potential for contamination of heavy metals in clayey soil with a history of the application of different types and amounts of fertilizers under Cynodon spp. grazing and no-tillage system for soybeans. In Experiment 1, Cynodon spp. were cultivated for hay production and submitted to applications of the following treatments: 0, 200, and 400 kg ha⁻¹ of total-N in the form of pig slurry and urea. In experiment 2, the soybean was grown and subjected to the following treatments: control, pig slurry, commercial granulated solid organic fertilizer, organomineral fertilizer blended with granules, and mineral fertilizer blended with granules. The dose of each nutrient source was determined based on the soil analysis and the recommendations of the liming and fertilization manual. The experiments were conducted for four agricultural crops where soil samples were collected in the layer of 0.00–0.10 m layer and submitted to chemical fractionation of Cu and Zn. The use of pig manure increased the availability of Cu and Zn in the soil, causing an increase in the distribution of copper organic (40.73% and 57.64% for PS200 and PS400 treatments, respectively, in Experiment 1; 67.33% for PS treatment, in Experiment 2) and residual fractions (10.01% and 7.60% for PS200 and PS400 treatments, respectively, in Experiment 1; 9.80% for PS treatment, in Experiment 2), while zinc was predominant in clay-mineral (61.04% and 92.01% for PS200 and PS400 treatments, respectively, in experiment 1; 278.90% for PS treatment, in Experiment 2) and residual fractions (2.36% for PS200 treatment in experiment 1; 9.90% for PS treatment, in Experiment 2). Additionally, the use of swine manure increased the Cu and Zn contents in the exchangeable fraction, which may potentiate the toxicity to plants and increase the potential for contamination of subsoil water. However, the increased bioavailability of Cu and Zn did not caused a loss of crop productivity but instead increased dry mass production and crop yield.
Sonication is widely used for nanomaterials dispersion in cementitious matrices. Polycarboxylate-based superplasticizer (SP) admixtures are usually incorporated in the aqueous nanomaterials suspension before sonication to improve the dispersion of these materials. Nevertheless, the effect of sonicating SP on its molecular structure or dispersing performance has not been fully investigated. This work assessed the effect of sonicating a commercial SP at 20 kHz, 750 W power, 50 or 80% amplitude, during 15 and 30 min. Initially, the sonication effect was evaluated in aqueous suspension by determining the SP size distribution (through dynamic light scattering-DLS) and zeta potential. Subsequently, the aqueous SP suspensions were used for Portland cement paste production. Rheological tests up to 120 minutes and compressive strength at 14 and 28 days were conducted. DLS and zeta potential results suggested that sonication reduce the size of SP chains. As a result, SP sonication increased the initial dynamic yield stress, viscosity, and hysteresis area of cement pastes. In contrast, SP sonication improved its time-dependent dispersing performance, resulting in pastes with reduced viscosity from 80 minutes onwards. Finally, SP sonication did not affect the compressive strength of cement pastes at 14 and 28 days of hydration. Overall, when SP is sonicated together with nanoparticles for application in cementitious matrices, the effect of sonicating the chemical admixture must be considered when the fresh-state properties of the composite are evaluated.
Methylglyoxal (MGO) is a reactive dicarbonyl compound formed as a byproduct of glycolysis. MGO is a major cell-permeant precursor of advanced glycation end products (AGEs), since it readily reacts with basic phospholipids and nucleotides, as well as amino acid residues of proteins, such as arginine, cysteine, and lysine. The AGEs production induced by MGO are widely associated with several pathologies, including neurodegenerative diseases. However, the impact of MGO metabolism and AGEs formation in the central nervous system (particularly in neurons, astrocytes and oligodendrocytes) on behavior and psychiatric diseases is not fully understood. Here, we briefly present background information on the biological activity of MGO in the central nervous system. It was gathered the available information on the role of MGO metabolism at the physiological processes, as well as at the neurobiology of psychiatry diseases, especially pain-related experiences, anxiety, depression, and cognition impairment-associated diseases. To clarify the role of MGO on behavior and associated diseases, we reviewed primarily the main findings at preclinical studies focusing on genetic and pharmacological approaches. Since monoamine neurotransmitter systems are implicated as pivotal targets on the pathophysiology and treatment of psychiatry and cognitive-related diseases, we also reviewed how MGO affects these neurotransmission systems and the implications of this phenomenon for nociception and pain; learning and cognition; and mood. In summary, this review highlights the pivotal role of glyoxalase 1 (Glo1) and MGO levels in modulating behavioral phenotypes, as well as related cellular and molecular signaling. Conclusively, this review signals dopamine as a new neurochemical MGO target, as well as highlights how MGO metabolism can modulate the pathophysiology and treatment of pain, psychiatric and cognitive-related diseases.
Eurytrema coelomaticum are trematodes that parasitize cattle, buffaloes, goats, sheep, and camelids, and, accidentally, humans and cats. The affected animal usually has a subclinical pancreatic disease, but the damage caused to health and animal production is underestimated. Currently, praziquantel is the unique drug proven effective against this parasite, but no formulations containing this drug are available for ruminants in Brazilian market. The objective of the present study was to separately evaluate in vitro anthelmintic activity of praziquantel (PZQ) and nitazoxanide (NTZ) against E. coelomaticum by assessing motility after 3, 12, and, 15 hours of incubation and subsequent histopathological examination of the parasites. E. coelomaticum specimens were obtained from the pancreas of naturally infected cattle, collected from animals slaughtered in the city of Concordia-SC, Brazil. The specimens were incubated in plates with a culture medium (n = 60 per group), with 80µg/ml of PZQ (positive control group), or 61,5µg/ml of NTZ (treated group), or UNT (untreated control). After 12 hours of incubation all parasites of the NTZ and PZQ groups were motionless or dead, while in the negative control group, 91.67% (55/60) presented normal motility after 15 hours of incubation (P < 0.001). Histopathological examination showed severe damage in vitellogenic gland, intestine, parenchyma, integument, and testicle in both treated and positive control groups. It was concluded that PZQ and NTZ showed in vitro anthelmintic action against the parasite Eurytrema coelomaticum, as they caused significant lesions in the evaluated organs and reduced the parasite’s motility.
Resumo Objetivo conhecer a experiência de profissionais e residentes atuantes no centro obstétrico acerca da utilização do plano de parto. Método pesquisa qualitativa, desenvolvida com sete profissionais e cinco residentes atuantes em um centro obstétrico de um hospital de ensino localizado no sul do Brasil. A coleta de dados ocorreu por meio de um questionário semiestruturado, no período de novembro a dezembro de 2020. Os dados obtidos foram submetidos à análise temática de conteúdo. Resultados a carência de conhecimento ou de atualização surgiu como um dos motivos para a não utilização do plano de parto, além do dimensionamento inadequado para atender às demandas do serviço. Entre as possibilidades para a utilização do plano de parto, têm-se a elaboração durante as consultas de pré-natal e a atuação de uma equipe multiprofissional. Conclusão e Implicações para a Prática a busca pelo conhecimento é a chave principal para aumentar a viabilização e, consequentemente, a utilização desse documento durante o pré-natal e trabalho de parto. O conhecimento acerca da utilização do plano e parto promove condições para o exercício da autonomia e protagonismo da mulher durante o trabalho de parto, parto e nascimento.
In this study, coatings of pectin crosslinked with Ba 2+ or Ca 2+ ions were prepared on sheets of AZ31 magnesium alloy, and their properties were investigated by means of infrared spectroscopy, electronic microscopy, energy dispersive spectroscopy, contact angle measurements and swelling essays. The corrosion behavior of the coated samples was characterized by means of electrochemical impedance spectroscopy. The prepared coatings were dense, with a thickness of ca. 13 μm and reached initial impedances in the order of 10 5 Ω cm 2. Additionally, it is demonstrated that coatings crosslinked with Ba 2+ present lower swelling and better corrosion protection in the first days of exposure to the corrosive solution. All these results indicate that pectin is a suitable polymer for the preparation of efficient, sustainable and biodegradable coatings for Mg alloys.
Resumo O uso de polímeros na indústria está em expansão, principalmente o de polímeros reforçados com fibra de vidro (PRFV). Na produção de peças com PRFV, inevitavelmente, são geradas quantidades significativas de resíduos, que carecem de destino em aterros industriais, o que representa um custo para o setor industrial e um problema ambiental. A busca por soluções que possam incorporar esses resíduos no processo ou como substituinte de matérias-primas é um dos focos atuais de pesquisas na área de materiais. Assim, este estudo avaliou a aplicação do resíduo de PRFV em misturas de concreto para a produção de peças para pavimento intertravado. O resíduo apresentou granulometria de agregado miúdo, com módulo de finura de 1,71. A morfologia revelou haver uma superfície heterogênea, com grânulos de diferentes tamanhos e bastonetes alongados lisos. A substituição parcial da areia pelo resíduo de PRFV (5%, 10% e 15%) resultou em peças com menor resistência à compressão (< 31 MPa) quando comparadas ao controle, sem PRFV. Entretanto, os resultados de resistência permitem a aplicação dos pavers em lugares com tráfego leve ou mesmo em calçadas, com a vantagem de a peça ser mais leve.
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14,854 members
Robert Wayne Samohyl
  • Departamento de Engenharia de Produção e Sistemas
Sayonara Barbosa
  • Departamento de Enfermagem
Vinicius Albani
  • Departamento de Matemática
Maria Luiza Bazzo
  • Departamento de Análises Clínicas
Natália Mezzomo
  • Departamento de Engenharia Química e Engenharia de Alimentos
Campus Universitário, 89520-000, Florianópolis, Santa Catarina, Brazil
Head of institution
Ubaldo César Balthazar
+55 (48) 3721-9000