Numerous diseases could be potentially treated using gene therapy based strategies. An ideal gene therapy system should lead to a safe, low toxicity, long termed and high-level of expression of the therapeutic protein. However, the scaling up of the production for its application to general recombinant viral vector clinical treatment in humans presents a big problem for some of them, for example
... [Show full abstract] the third generation adenovirus. Focusing on the improvement of the high-level transgene expression of an adenoviral vector, we characterized a simple and efficient technique to transduce the liver.