Yusaku Miyamae's research while affiliated with University of Tsukuba and other places
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Publications (6)
Background
Viral vectors are attractive gene delivery vehicles because of their broad tropism, high transduction efficiency, and durable expression. With no risk of integration into the host genome, the vectors developed from RNA viruses such as Sendai virus (SeV) are especially promising. However, RNA-based vectors have limited applicability becau...
We established orthogonal deubiquitination, which enables us to selectively observe a single type of deubiquitinating enzyme activity in living cells.
Hepatocyte growth factor (HGF) is expressed in various organs and involved in the fundamental cellular functions such as mitogenic, motogenic, and morphogenic activities. Induction of HGF may be therapeutically useful for controlling organ regeneration, wound healing, and embryogenesis. In this study, we examined the stimulation effect of cyanidin...
Proteins are fundamental biomolecules of living cells, and their expression levels depend on the balance between the synthesis and degradation. Researchers often aim to control protein expression levels for the investigation of protein function and its relationship with physiological phenomena. The genetic manipulation of the target protein using C...
Citations
... C3G (50 μM) or Lip-1(0.5 μM) were added, respectively, and incubated with Era. According to the manufacturer and refer to published literatures (Shan et al. 2021;Krama et al. 2022;Li et al. 2020;Qin et al. 2018b), we finally determined a relatively low dosage and timing of C3G and final concentration and timing of CC. For in vivo experiments, the dosage of the drugs was C3G (10 mg/kg) and CC (10 mg/kg). ...
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... Cumate was designed as a small molecule to regulate gene expression in mammalian cells [48] by facilitating interaction between a bacterial CymR repressor protein from Pseudomonas putida and its synthetic operator DNA sequence (p-cym and pcmt operons). This interaction can either activate (Cumate-On) or suppress (Cumate-Off) gene expression in mammalian cells. ...
