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3 Citations
Plasmids and their derived gene cloning and expression vectors play a prominent role in almost all area's of molecular genetics. A disadvantage, however, is the frequently observed high level of instability of plasmids, especially when they contain heterologous DNA. Two types of instability can be distinguished: structural and segregational instability. ... Zie: Chapter 10
... Based on sequence similarity of their DSOs and homology of the replication proteins, RCR plasmids from Gram-positive bacteria can be divided into ¢ve classes, represented by pT181, pC194, pE194, pSN2, and pTX14-3 [1,45]. Based on these criteria the four pTA plasmids belong to the pC194 class of plasmids. ...
