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- 2Does anyone know how to interpret PSS-Fa Scale?
Perceived Social Support from the Family Scale
Yes, your answer was helpful. Thank you!Following
- 4Do you have any suggestions for an efficient software to coregister two MRI volumes?
I have two volumes: one structural MRI, and a functional NIFtii atlas. And I need to coregister these two volumes.
What can be the suitable software to do this task (slicer, freesurfer)? Slicer is a friendly software with a GUI, but I don't know how accurate it is.
What are your suggestions?
Thank you all.
@ Ali, I heard a lot about FSL software you proposed. I will try it very soon
@ Mauro, thank you a lot. I tried SPM and it works well for this task.
@ Salem, thank you!Following
- 2How can I get Acoustic Mode shapes in Ansys? How can I calculate FEA based Fatigue life from ANSYS?
Pipe Upstream Pressure = 47.5 bar
Pipe Downstream Pressure = 10.31 bar
Pipe diameter = 457 mm
Pipe thickness = 6.35 mm
please find the Attached file..
Thank you sir....and How can I get that Mode Shapes?Following
- NewKindly can i be able to get any document by Colson E,2003. Forced migration and anthropological ?
Kindly assist me access any material by Colson E on forced migration and Marcus,GE(anthropology through thick and thin - princeton University)?Following
- 2Hi everyone please can you tell me how to calculate Jacobson's constant for inter-molecular free length Lf?
I need to calculate intermolecular free length Lf, I know the question but I am not able to find out how to calculate Jacobson's constant.
Dear Sir. Concerning your issue about how to calculate Jacobson's constant for inter-molecular free length Lf . You can visit the following link and the file attached which may help you in your analysis:
- 1How to calculate Hubbard U parameter for different atoms in a given compound?
I am using Quantum espresso, I would like to know about calculating Hubbard U parameter using ACBN0 scheme for different atoms in a compound. since the selection of correct U and J parameter are necessary to find electronic structure and magnetization values. It will be more helpful if we can generate U values.
probably this tutorial may be helpful in your case.
- 2How can I explain why the thickness of porous TiO2 films is three times smaller than the grain size of anatase?
TiO2 porous films were deposited on ITO substrates by spin coating technique. I have found the thickness of porous TiO2 films is three times smaller than grain size of anatase. How can I explain this phenomenon?
Thank you DR Tobias Deutschmann
I used AFM to get thickness . Films were heat treated at 550°C for 2 hoursFollowing
- 2What is the most effective way to avoid interference from the heavy chain of IP antibody in the IP:WB?
I am thinking that choosing the IP antibody from a specie different from that used as primary and secondary antibodies during the IP western blot is the way to go i.e mice IP ab/rabbit primary ab/ anti rabbit secondary ab or rabbit IP/mouse primary/ anti mouse secondary.
Thanks Jordan, Option 2 will be looked into as I am already using 0.1M Glycine pH 2 for elution.The question then arises if the heavy and light chains of the IP antibody have their epitops denatured beyond recognition why do they still show up on the blot? How does the secondary 'recognize' them?Following
- 63How should / could we improve the Educational System, to adapt modern times, and future generations?
Most would agree that Education plays a fundamental role in solving many of the humanity's current problems.
I believe in a better World.
Mass education for all youngster can play a good role in the near future.
Should we change our system?
Should we act on University level?
Should we act transversally from kindergarten, through basic education?
I should like your valuable contributes.Following
- 2What is the definition of a quadrilateral?
In the glossary at the end of the attached paper, I define a quadrilateral as:
Quadrilateral The union of two triangles adjacent on their longest sides
1. Kite The two triangles are congruent and uncommon sides that are equal are also consecutive
2. Parallelogram The two triangles are congruent and uncommon sides that are equal are also opposite
3. Rectangle A quadrilateral with all equal angles
4. Rhombus A quadrilateral with all equal sides; plural, rhombi
5. Square A quadrilateral with all equal angles and sides
I do not define the term trapezoid but instead define the triangle frustum:
1. Triangle The part of a triangle between the base and a parallel segment called the top
2. Circle The part of a circle between it and a chord
Later, when I introduce solid geometry, frustum will be naturally extended to solids whose tops have been cut off with a plane parallel to the base. Observe that the term "segment" does not mean what I call a circle frustum. It has only one meaning:
Segment All the points along the shortest path between two points
Do you know of a better way to define a quadrilateral? If so, why do you feel that it is better?
- 3How do i solve my problem if there is no expression of protein after site directed mutagenesis experiments?
I have done site directed mutagenesis in a 4kb plasmid(pQE31) having gene(500bp) encoding 18kDa protein using Quick change lightning site directed mutagenesis kit from Agilent. Transformed the PCR product into XL gold competent cells. Everything worked fine, after that i sequenced my DNA and confirmed the mutation at 52nd amino acid position. When I transformed the DNA into my expression strain M15 E coli cells there was no expression of the 18kDa protein. my wild type protein was also expressed in the same cells using 0.4mM IPTG. I have tried different concentrations of IPTG and low temperature. Also I confirmed no other insertion of stop codons within the sequence. Also there is no change in the promoter and other sequences. what may be the problem?
I assume you confirmed the desired mutation by getting your gene of interest (GOI) sequenced. Most likely you did not get sequenced the promoter/operator region upstream your GOI. It is not impossible to get undesired secondary mutations in the plasmid vector that contains your GOI. Since you have already got at-least one clone with desired mutation, simply use it to replace the WT gene in the plasmid vector that is correctly expressing the WT gene. You can do it by excising out the WT (and mutated) gene using appropriated restrictin enzymes and subsequent ligation. Good luck!Following
- 9Which method is suitable for spaying in dogs? Right flank approach or Mid Ventral approach?
The best way to do spaying in dogs especially in stray dogs either by right flank or Mid-ventral???
Dr. Mehrajuddin Naikoo: Excellent, Have you tried Rt. Flank. If not try for consecutive 3 times and pls share your experience.Following
- NewI want to know about the natural flora of Kolar Gold Fields i.e. before the mines were systematically explored.
I intend to gather information about the plants which existed before the KGFs were mined systematically.Following
- 10In your opinion who are the nurses with more academic and research influence on the English primary care from 1940 to the present?
I am preparing a review of the subject and need information.
Thanks for all the replies.Following
- NewWhat will be the minimum criteria for using a molecule in direct alcohol fuel cell (DAFC)?
What will be the minimum criteria (like performing some definite chemical reaction) for using a molecule in DAFCs? Please consider Grutzmacher complex.Following
- 14Who is a sycophant? Is sycophancy an Art?
We see sycophants in all the fields - political, social, administrative ......
Do you think it is an art that is harmless or something that harms the organisational culture severely.
What kind of sycophants are prevalent in your part of the world.
"How can he [today's writer] be honored, when he does not honor himself; when he loses himself in the crowd; when he is no longer the lawgiver, but the sycophant, ducking to the giddy opinion of a reckless public."
----- Ralph Waldo EmersonFollowing
- 13The types of complications associated with diabetes differs between individuals. What are the reasons?
Regarding the complications associated with diabetes, what is the less understood consequence of prolonged hyperglycemia acting differently in different individuals?
Dear Ali Merina Houria , hope you find these references of fulfilling your interest.
Bashir, A.H.H. (2004). Clinico-epidemiological Study of Cutaneous Manifestations of Diabetes mellitus in Jabir Abu Eliz Diabetic Center in Khartoum, Sudan. Paper, Collage of Medicine, University of Juba, Sudan- Sudanese Journal of Dermatology. September 2004; 2(2):34-38.
1. National Diabetes Data Group 1979.Classification and diagnosis of diabetes mellitus and other categories of glucose intolerance. Diabetes; 28:1039-57.
2.Perez M I, Kohn SR 1994.Cutaneous manifestations of diabetes mellitus. J Am Acad.Dermatol; 30:519-31.
3.OyerDS 1982.Diabetes mellitus: new developments. Arch.
4. Diabetes Statistics-MSN Home/Hotmail-For the latest MSNBC Health. Internet.
5. Sonck CE, Somersalo O 1963: The yeast flora of the anogenital region in diabetic girls. Arch. Dermatol. 88:846-852.
6. Knight L, Fletcher J 1971: Growth of Candida albicans in saliva: Stimulation by glucose associated with antibiotics, corticosteroid, and diabetes mellitus. J Infect Dis 123:371-377.
7. Lugo-Somolinos A, Sanchez JL 1992: Prevalence of dermatophytosis in patients with diabetes: J. Am. Acad. Dermatol. 26:408-410.
8.Alteras I, Saryt E 1979: Prevalence of pathogenic fungi in the toe-webs and toe-nails of diabetic patients. Mycopathologia 67:157-159,.
9. Cole GW, Headley J, Skowsky R (1983): Scleredema Diabeticorum: A common and distinct cutaneous manifestation of diabetes mellitus. Diabetes Care 6:189-192.
10. West KM 1978: Epidemiology of diabetes and its vascular lesions. New York, Elsevier North-Holland Inc. p 353.
11.Huntley AC, Walter RM Jr 1990: Quantitative determination of skin thickness in diabetes mellitus: relationship to disease parameters. Journal of Medicine, , 21(5):257-64.
12. Blackwell science Ltd. Rook/Wilkinson/Ebling 1998. Textbook of dermatology. Sixth edition.
13. Gupta AK, Humke S. The prevalence and management of onychmycosis in diabetic patients. University of Toronto, Toronto, Canada.
14. Murphy RA 1965: Skin lesions in diabetic patients: The “spotted leg” syndrome. Lahey Clin Found Bull 14:10-14.
15.Bear CL, Wall LN 1969. Idiopathic bullae in diabetics. Australas J Dermatol; 10: 337.
16. Derighetti M, Hohl D, Krayenbuhl BH, Panizzon RG. Bullosis diabeticorum in a newly discovered type 2 diabetes mellitus. Department of Dermatology, DHURDV, CHUV, Lausanne, Switzerland.
17. A Huntley December 1995. Photo-essay: The Skin and Diabetes Mellitus. Dermatology Online Journal, Volume 1, Number 2.
18.Boyd-AS; Neldner-KH 1991 Oct. Lichen planus, Department of dermatology, Texas Tech University Health Sciences Centre, Lubbock 79430. J-AM-Acad-Dermayol.; 25(4) :593-619.
19. Karvonen SL, Haapasaari KM, Kallioinen M, Oikarinen A, Hassinen IE, Majamaa K 1999 Apr. Increased prevalence of vitiligo, but no evidence of premature ageing, in the skin of patients with bp 3243 mutation in mitochondrial DNA in themitochondrial, encephalomyopathy, lactic acidosis and stroke-like episodes syndrome (MELAS). Br J Dermatol; 140(4): 634-9.Following
- 3Why Intensity of XRD peak increases by milling powders in dry medium?
Generally after milling of crystalline material, decrease in intensity of the XRD peak is observed indicating decrease in crystallinity, due to milling for longtime. At higher rpm, peak narrowing and increase in intensity is observed, what can be the reason behind it.
Thank you Alexandre and R. Taherzadeh...It would be helpful if you can throw some light on, how to identify the preferential plane exposure during milling?Following
- 9How has your university changed as free market principles are more apparent in your institutions?
As neo-liberalisme policies are becoming a prominent feature in Higher education, I'm interested to see the perception of students and staff. in regards to its impact [ in terms of curriculum, teaching & learning, research [quantity vs quality] etc]
Thanks guys for all the contributions. Here in Malaysia we see familiar patterns that is worrying....Following
- 2Which aspect / application of PMO in construction industry requires further research?
I am doing Masters in Construction Project Management from University of Liverpool. I am now searching for an appropriate issue for Dissertation research relating to PMO in the construction industry.
Hi Syed Ehtesham Husain,
My suggestions would be related to organisational level:
- Challenges in/Barriers to implementing an effective PMOs in Construction Industry - You can undertake a good and thorough literature review and use questionnaire and possibly interview to deliver your dissertation
- PMO: What are the benefits? Theory vs Reality - you may use interview and questionnaire as your tools.
Good luck Syed Ehtesham Husain. :)Following
- 7Is there any way to measure the pH of an acidified Acetonitrile solution?
I wanna do some pH dependence study of absorption and fluorescence spectra.
Dear Sir. Concerning your issue for the way to measure the pH of an acidified acetonitrile solution in absorption and fluorescence spectra you can visit the following link and the file attached which may help you in your analysis:
- NewAOA, I want to prepare a research paper about theory of Computation, please tell me related topics on which i shall do a fruitful research?
Please tell me topic related to "Theory of computation" on which i make a research to complete my research paper?Following
- 8Can someone help me for the propagation of a cohesive crack in franc2d?
I've some problem with franc2d 3.1. I want to propagate a cohesive crack, i defined the material properties and the properties of the non linear interface. when i want to propagate the fracture the program ask me the number of interaction and the precision of the solution. I insert this values and after that the program collapse.
what i can do?
there are some other open source software for model cohesive crack?
Forgive me for my english.
I have defined the load, the crack and the fixity in the file m2.wdb. I tried to dowload and open it and these conditions were there. it can be some problem with the version of sowthare? i'm working with v3.0
- 5What statistical tests we can conduct to understand the differences in geochemical processes among sites?
Which all statistical tests should be carried out for interpreting geochemical data set to find differences among sites, seasons, metal species, ionic bonding, etc.
@Donald A Singer: Thanks for your kind reply.Following
- NewWhat is the rate equation for galactose oxidase?
Please let me know the rate equation for galactose oxidase. If two molecules are attached in TS. Is it possible that rate is dependent on both the molecules?Following
- 2Do you consider Extra digestive Helicobacter pylori skin mafestations, EdHpSm as maker to gastric disease condition?
Helicobacter pylori are gram-negative; microaerophilic spiral rod-shaped bacteria and they lead to gastritis, duodenal or gastric ulcer and even in rare cases to gastric carcinoma or Mucosa Associated Lymphoid Tissue (MALT) lymphoma. Based on a number of reports, a possible relationship of H. pylori infection to a variety of different dermatosis has been suggested, including urticaria, rosacea, acnerosacea, atopic dermatitis, alopecia areata, Sjögren’s syndrome, Schönlein-Henoch purpura, and Sweet’s syndrome.
Dear Dvoryanchikovو Thanks for sharing discussion as a lot of controversy is raising as regards H. pylori pathogenicity and the the question of; is a commensal cause Adenocarcinoma and MALT lymphoma which is proved by WHO as 6 fold carcinogenic to guard against GERD?! Helicobacter pylori are gram-negative, microaerophilic spiral rod-shaped bacteria that live just beneath the antral
gastric mucous layer, on the surface of epithelial cells. Stomach infection with this organism causes inflammation of the gastric mucosa, which can lead to gastritis,
duodenal or gastric ulcer and even in rare cases to gastric carcinoma or Mucosa Associated Lymphoid Tissue (MALT) lymphoma (Covacci et al., 1999). Approximately 50% of the world's population is believed to be infected with H. pylori. Most infections are probably acquired in childhood (Czesnikiewicz-Guzik et al., 2004), but geographic locale, age, race, socioeconomic status, and hygiene seem to play roles in the prevalence of H. pylori. Most of the informations about H. pylori infection rates come from seroprevalence studies. Higher rates of
infection tend to occur at a younger age in developing countries compared to developed countries and in regions characterized by lower socioeconomic status and higher density living.1 Looking at racial differences in the US, it is found that whites of non-Hispanic origin have lower prevalence of infection compared to African- Americans or Hispanics (Brown, 2000). It has been speclated that dental plaque might harbor H. pylori and, therefore, might be a source of gastric infection
(Czesnikiewicz-Guzik et al., 2004). Aside from iatrogenic transmission of H. pylori via endoscopy, no definite modes of transmission for H. pylori have been identified.
However, association studies suggest that there are three other potential routes for the transmission of H. pylori: person-to-person transmission (example, oral-oral or
fecal-oral), waterborne transmission (example, contaminated water), zoonotic (example, cats and other pets and animals) or vector borne transmission (example, flies). Person-to-person transmission is considered to be the most likely route of transmission considering that isolation of H. pylori from non-human reservoirs has been inconsistent (Brown, 2000). Chronic urticaria (CU) and
concurrent angioedema are frustrating problems for both physicians and patients (Wedi et al., 1998). Based on a number of reports, a possible relationship of H. pylori infection to a variety of different dermatosis has been suggested, including urticaria, rosacea, acne-rosacea, atopic dermatitis, alopecia areata, Sjögren’s syndrome, Schönlein-Henoch purpura, and Sweet’s syndrome. Larger case-control studies, however, do not confirm this relationship. Therefore, H. pylori eradication therapy cannot be generally recommended in this dermatosis
(Boni et al., 2000). Pathogenic strains of H. pylori have been shown to activate the epidermal growth factor receptor (EGFR), a membrane protein with a tyrosine kinase domain. Activation of the EGFR by H. pylori is associated with altered signal transduction and gene expression in host epithelial cells that may contribute to pathogenesis (Tomb et al.,1997).
Auriemma L, Signorelli S (2001). The role of tetracycline in the
retreatment after Helicobacter pylori eradication failure. Minerva
Med., 92(3): 145-149.
Boni R, Burg G, Wirth HP (2000). Helicobacter pylori and skin
diseases--a (still) intact myth? Schweiz. Med. Wochenschr.,
Brown LM (2000). Helicobacter pylori: epidemiology and routes of
transmission. Epidemiol. Rev., 22: 283-297.
Covacci A, Telford, JL, Del Gludice G, Parsonnet J, Rappuoli R
(1999). Helicobacter pylori virulence and genetic geography.
Science, 284: 1328–1333.
Czesnikiewicz-Guzik M, Karczewska E, Bielanski W, Guzik TJ,
Kapera P, Targosz A, Konturek SJ, Loster B (2004). Association of
the presence the Helicobacter pylori in the oral cavity and in the
stomach. J. Physiol. Pharmacol., 55 Suppl 2: 105-15.
Ivashkin VT, Lapina TL, Bondarenko OY, Sklanskaya OA, Grigoriev
PY, Vasiliev YV, Yakovenko EP, Gulyaev PV, Fedchenko VI
(2002). Azithromycin in a triple therapy for H. pylori eradication in
active duodenal ulcer. World J. Gastroenterol., 8(5): 879-882.
Tomb JF, White O, Kerlavage AR, Clayton RA, Sutton GG,
Fleischmann RD, Ketchum KA, Klenk HP, Gill S, Dougherty BA,
Nelson K, Quackenbush J, Zhou LX, Kirkness EF, Peterson S,
Loftus B, Richardson D, Dodson R, Khalak HG, Glodek A, Vaira D,
Holton J, Menegatti M, Ricci C, Landi F, Ali A, Gatta L, Acciardi C,
Farinelli S, Crosatti M, Berardi S, Miglioli M (1999). New
immunological assays for the diagnosis of Helicobacter pylori
infection. Gut., 45 Suppl. 1: I23-127.
Wedi B, Wagner S, Werfel T, Manns MP, Kapp A (1998). Prevalence
of Helicobacter pylori-associated gastritis in chronic urticaria. Int.
Arch. Allergy Immunol., 116(4): 288-294.
Wieczorek D, Raap U, Liekenbrocker T, Kapp A, Wedi B (2004).
Chronic urticaria in childhood. Hautarzt., 55(4): 357-360.
Bashir, A.H.H. Clinico-epidemiological study in Sudanese patients, Prevalence and effect of eradicative triple therapy on extra digestive Helicobacter pylori skin manifestations, EdHpSm. Clinical Reviews and Opinions Vol. 3(2), pp. 14-19, February 2011.Following
- 1How do I extract cytosolic and nuclear proteins in their native state?
I need to extract cytosolic and nuclear proteins in their native state (without using any denaturing or reducing agents). I'm using the following extraction buffer: 20 mM Tris-HCl pH 8.0, 2 % NP-40, 1 mM EDTA, and 1 mM PMSF (probably a protease inhibitor cocktail instead of PMSF) to get the cytosolic proteins. However, since NP-40 doesn't solubilize the nuclear membrane, I need an addition to my protocol to get the nuclear proteins. The caveat is that if I use RIPA for example, the detergent it contains is ionic and will denature the nuclear proteins. Any suggestions what I can use to get the nuclear proteins in their native state?
Kindly go through the attached article. Hope it will help.Following
- NewCan someone help me with a simple methodology for modelling of possible leachate contamination from a dumpsite?
I am currently working on possible contamination of a shallow unconfined aquifer from a municipal solid waste dumpsite. I am using both geophysical and geochemical data and I hope to model leechate contamination transport using Mud path /mud flow software.Following
- Aslanbek Naziev added an answer in Elementary logic:1Look at the following table. Is it true that for every unit x and every unit y there exists a zero lying between x and y?
1 0 1
0 0 0
1 0 1
Hint: This is not a problem from matrix algebra or, moreover, matrix analysis. This is a problem from elementary logic!
For more than 1 day this question collected only 2 views. Maybe the answer is absolutely evident for all, but my practice tells me that many people give to this question wrong answer, namely, the "evident" one! That was the reason for asking this question.Following
- 9Can anybody identify this seawater benthic copepod?
Sorry for the low photography quality, definitely a benthic harpacticoid, but I can't tell the genre.
If anybody could suggest bibliography or identification keys for benthic copepods as well, it would be great!
some keys for the Black and Azov Sea, but note that both have low salinity !Following