Q&A

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  • Nancy Duarte added an answer in 2D-DIGE:
    1
    Does anyone have experience with depleting proteins in plasma by using 'Pierce Top 12 Abundant Protein Depletion Spin Columns' from Thermo Scientific?

    We want to remove the high abundance proteins in plasma by using 'Pierce Top 12 Abundant Protein Depletion Spin Columns' so that we can use this plasma for 2D-DIGE. Thermo Fisher advises max 10 µl plasma / column, has anyone tried to add more than 10 µl (for example 20 µl)? Thank you in advance!

    Nancy Duarte

    I have used an albumin and IgG depletion column from BioRad. But what I have to say may help you. In 10µl of plasma you dont know the protein amount. If the protein concentration is high you may be overloading the depletion column.

    I recommend you to quantify the protein sample first and then standarize the amount to load in the column. With my columns I used 4mg of protein sample and it worked very well

  • James Weese added an answer in Diabetes:
    4
    How to treat pancreatogenic diabetes?
    I have a 60 y.o WF 5 years post total pancreatectomy secondary to cancer. Worsening hypoglycemia with minimal insulin. A1C 9.9%. Any recommendations? Metformin?
    • [Show abstract] [Hide abstract]
      ABSTRACT: Pancreatogenic, or type 3c, diabetes (T3cDM) occurs due to inherited or acquired pancreatic disease or resection. Although similar to the more prevalent type 1 and type 2 diabetes, pancreatogenic diabetes has a unique pattern of hormonal and metabolic characteristics and a high incidence of pancreatic carcinoma in the majority of patients with T3cDM. Despite these differences, no guidelines for therapy have been described. Published studies on the prevalence, pathophysiology, and cancer associations of T3cDM were reviewed. The recent studies on the protective role and mechanism of metformin therapy as both an anti-diabetic and anti-neoplastic agent were reviewed, and studies on the cancer risk of other anti-diabetic drugs were surveyed. T3cDM accounts for 5-10% of Western diabetic populations and is associated with mild to severe disease. Hepatic insulin resistance is characteristic of T3cDM and is caused by deficiencies of both insulin and pancreatic polypeptide. 75% of T3cDM is due to chronic pancreatitis, which carries a high risk for pancreatic carcinoma. Insulin and insulin secretagogue treatment increases the risk of malignancy, whereas metformin therapy reduces it. Pancreatic exocrine insufficiency associated with T3cDM contributes to nutritional deficiencies and the development of metabolic bone disease. Until consensus recommendations are reached, the glycemic treatment of T3cDM should avoid insulin and insulin secretagogues if possible. Metformin should be the first line of therapy, and continued if insulin treatment must be added for adequate glucose control. Pancreatic enzyme therapy should be added to prevent secondary nutritional and metabolic complications. and IAP.
      No preview · Article · Jul 2011 · Pancreatology
    James Weese

    I agree with all that has been said.  Having cared for many total pancreatectomy cases it is important to cover both their glucose and malabsorptive issues.  They should be on pancreatic enzymes with meals but an insulin pump with continuous glucose monitoring should be the safest way to avoid major shifts.

  • Daniel Martinez Krahmer added an answer in Mechanical Engineering:
    3
    According to Kinzle Equation how to calculate the cross section area of undeformed chip in face milling?

    the problem in the three parameters that depth of cut, chip thickness and width of chip

    Daniel Martinez Krahmer

    Finally to face milling the formula to calculate chip thickness si the following:

    h = (2*fz*sin X*B)/(phi*d) where:

    fz is the feed per tooth,

    X is the angle between the cutting edge and the surface edge,

    phi is the contact angle between the mill and the part, and finally 

    d is the diameter of the tool.

    You can find values for kz1 and m in many books. For example:

    Machining for chip cutting, Micheleti;

    Cutting tools part 1, Caspar Stemmer and many others. Good luck!!!!

  • U. Eze Peter asked a question in Parallel Algorithm:
    New
    For Recursive Parallel Algorithms, is there a way of managing the overhead incurred for large problem sizes, especially for OOP in Java?

    Insight into memory management, communication overhead, latency and other parameters for measuring the performance of parallel algorithms should be put into consideration while answering this question

  • Mohamed A. A. Mahdy added an answer in Peer Review:
    1
    Is it right to inform an editor that the reason why you won't review a paper is because you peer reviewed the same paper earlier for another journal?

    Assuming a paper you reviewed earlier is sent to you by an editor of another journal, is it right to inform the editor that you will not review it because you earlier did for another journal? Will such revelation affect the acceptance of the paper in the new journal?

    Mohamed A. A. Mahdy

    Dear Emeka

    First you should not submit your paper to more than one journal at the same time

    Second, every journal has his own reviewers and every reviewer has his point of view

    Sometimes reviewers reject the paper because of the experiment or content another reviewers in another journal accept with some modification

    it is not a rule that if your paper was reviewed so it should not be reviewed again. 

    Reviewer should ask you (if it had been reviewed why you didn't progress till publication???)

  • Yohan Farouz added an answer in Reference Manager:
    17
    Can someone suggest a reference management software?
    N-note, mendely, papers, jotoro, refworks etc.
    Yohan Farouz

    Hi everyone,
    last year, I presented EZPaperz for syncing Papers with your Android device via Dropbox. Well, this year, let me introduce Aziz, Lite! (https://goo.gl/xy6Uyu), an Android app for synchronizing big libraries from either Mendeley, Zotero or Papers via either Dropbox, Google Drive or OneDrive.

    With Aziz, Lite! we really try to solve unanswered limitations: for many researchers, the 2Gb free space on Mendeley's server is not enough (I, myself, need more than 10Gb). What if you wanted to place your files on another Cloud instead? Well, with Aziz, Lite!, you can choose between Dropbox, Google Drive or OneDrive instead.

    You can download the app directly from here: https://goo.gl/xy6Uyu

    And if you feel like you want to help me promote the app, don't hesitate spreading the word and following the app development on social networks:
    - Twitter: https://goo.gl/YZSbGU
    - Facebook: https://goo.gl/M233fX
    - Google +: https://goo.gl/0WFAAq

    More information and Youtube tutorials on : http://www.azizlite.net/

    Don't hesitate to send me your feedback.
    Cheers,
    Yohan,

  • Yohan Farouz added an answer in Research Papers:
    56
    What is the best reference management software?
    -
    Yohan Farouz

    Hi everyone,
    last year, I presented EZPaperz for syncing Papers with your Android device via Dropbox. Well, this year, let me introduce Aziz, Lite! (https://goo.gl/xy6Uyu), an Android app for synchronizing big libraries from either Mendeley, Zotero or Papers via either Dropbox, Google Drive or OneDrive.

    With Aziz, Lite! we really try to solve unanswered limitations: for many researchers, the 2Gb free space on Mendeley's server is not enough (I, myself, need more than 10Gb). What if you wanted to place your files on another Cloud instead? Well, with Aziz, Lite!, you can choose between Dropbox, Google Drive or OneDrive instead.

    You can download the app directly from here: https://goo.gl/xy6Uyu

    And if you feel like you want to help me promote the app, don't hesitate spreading the word and following the app development on social networks:
    - Twitter: https://goo.gl/YZSbGU
    - Facebook: https://goo.gl/M233fX
    - Google +: https://goo.gl/0WFAAq

    More information and Youtube tutorials on : http://www.azizlite.net/

    Don't hesitate to send me your feedback.
    Cheers,
    Yohan,

  • Тetiana Bilushchak added an answer in Archival Research:
    4
    How can you motivate one to undergo research?
    On the premise that Research is indispensable if one wants to be successful in his/her career, the research work is integrated in the curriculum. However, the majority of our students, even the faculty, find research work as a very tedious and painstaking process that many seek to avoid it.
    Тetiana Bilushchak

    For many of advanced of scientists an important stimulus
    the research activities is a benefits which they could bring
    to society, homeland.

  • Jakub Zawiła-Niedźwiecki added an answer in Performance Art:
    3
    Does anyone know of any exhibitions or projects that have centred around academic-themed art?

    Given the place of arts faculties in universities, is anyone aware of how academic issues have been expressed artistically, in traditional media, craft & design, performance art, digital media etc.?  There are plenty of views and ideals out there, and given the wealth of imagination & talent we have in our universities, I would be surprised if there had been no national or international events that did not set out to promote their expression.

    Please provide links or files that will direct us to any events or works you have come across  :-)

    Jakub Zawiła-Niedźwiecki

    I am about as far from "artistic" as you can get when doing philosophy nad bioethics, but I appreciate that it may be a way to express some finer details that are hard to present in typical scholarship. I also appreciate that conferences have become, largely, a academic tourism and not really scholarly events. Actually most scholarship (probably about 95%) I have done on the several I attended last year was over dinner. I'm quite curious to see what these will be at IAB if my abstract gets accepted there. The entertainment part may have it's pluses, it's the same reason people still put jokes even into mathematical and logic papers.

    Also, on the less serious note, I am sure you know the 'Dance your PhD initiative'. Some of those are really inventive! (link below)

  • Yohan Farouz added an answer in Reference Manager:
    13
    Beside EndNote does anyone know other Reference manager?
    Beside EndNote does anyone know other Reference manager?
    Yohan Farouz

    Hi everyone,
    last year, I presented EZPaperz for syncing Papers with your Android device via Dropbox. Well, this year, let me introduce Aziz, Lite! (https://goo.gl/xy6Uyu), an Android app for synchronizing big libraries from either Mendeley, Zotero or Papers via either Dropbox, Google Drive or OneDrive.

    With Aziz, Lite! we really try to solve unanswered limitations: for many researchers, the 2Gb free space on Mendeley's server is not enough (I, myself, need more than 10Gb). What if you wanted to place your files on another Cloud instead? Well, with Aziz, Lite!, you can choose between Dropbox, Google Drive or OneDrive instead.

    You can download the app directly from here: https://goo.gl/xy6Uyu

    And if you feel like you want to help me promote the app, don't hesitate spreading the word and following the app development on social networks:
    - Twitter: https://goo.gl/YZSbGU
    - Facebook: https://goo.gl/M233fX
    - Google +: https://goo.gl/0WFAAq

    More information and Youtube tutorials on : http://www.azizlite.net/

    Don't hesitate to send me your feedback.
    Cheers,
    Yohan,

  • Sayyed Faramarz Tayyari asked a question in FT-Raman:
    New
    Where it is possible to buy a polarizer device for a FT Raman spectrophotometer equipped wit Nd:Yag lazer source?

    We need a polarizer devise to measure the depolarization ratio.

  • Yohan Farouz added an answer in Reference Manager:
    4
    What is the best reference management software out there?
    Mendeley is ok for local management but doesn't sync well across different devices with file.
    Readcube is alright for citation links but fails on local file management.
    Yohan Farouz

    Hi everyone,
    last year, I presented EZPaperz for syncing Papers with your Android device via Dropbox. Well, this year, let me introduce Aziz, Lite! (https://goo.gl/xy6Uyu), an Android app for synchronizing big libraries from either Mendeley, Zotero or Papers via either Dropbox, Google Drive or OneDrive.

    With Aziz, Lite! we really try to solve unanswered limitations: for many researchers, the 2Gb free space on Mendeley's server is not enough (I, myself, need more than 10Gb). What if you wanted to place your files on another Cloud instead? Well, with Aziz, Lite!, you can choose between Dropbox, Google Drive or OneDrive instead.

    You can download the app directly from here: https://goo.gl/xy6Uyu

    And if you feel like you want to help me promote the app, don't hesitate spreading the word and following the app development on social networks:
    - Twitter: https://goo.gl/YZSbGU
    - Facebook: https://goo.gl/M233fX
    - Google +: https://goo.gl/0WFAAq

    More information and Youtube tutorials on : http://www.azizlite.net/

    Don't hesitate to send me your feedback.
    Cheers,
    Yohan,

  • Yohan Farouz added an answer in Reference Manager:
    21
    Which reference manger to choose , Mendeley or Papers?
    I would like to organise lot of PDF's sitting in my PC. As both Papers and Mendeley are avaialble for windows desktop, I need valuable suggestion (Pros and cons) from the RG users as I need to choose one them for my PC.
    Yohan Farouz

    Hi everyone,
    last year, I presented EZPaperz for syncing Papers with your Android device via Dropbox. Well, this year, let me introduce Aziz, Lite! (https://goo.gl/xy6Uyu), an Android app for synchronizing big libraries from either Mendeley, Zotero or Papers via either Dropbox, Google Drive or OneDrive.

    With Aziz, Lite! we really try to solve unanswered limitations: for many researchers, the 2Gb free space on Mendeley's server is not enough (I, myself, need more than 10Gb). What if you wanted to place your files on another Cloud instead? Well, with Aziz, Lite!, you can choose between Dropbox, Google Drive or OneDrive instead.

    You can download the app directly from here: https://goo.gl/xy6Uyu

    And if you feel like you want to help me promote the app, don't hesitate spreading the word and following the app development on social networks:
    - Twitter: https://goo.gl/YZSbGU
    - Facebook: https://goo.gl/M233fX
    - Google +: https://goo.gl/0WFAAq

    More information and Youtube tutorials on : http://www.azizlite.net/

    Don't hesitate to send me your feedback.
    Cheers,
    Yohan,

  • Yohan Farouz added an answer in Bibliography Manager:
    25
    What are good tools for bibliography management?

    After a lengthy hiatus I'm back to reading scientific publications in bulk. Previously I would have used BibTex to manage the list of papers I've read and the notes fields to record a summary and my thoughts for further reference. However this now feels very clunky and unmanageable. I'm looking for something that supports more structured notes (something like emacs org-mode), rich text and could export selected references into common formats (eg BibTex) when I work on publications.

    I'd be interested to hear how other people handle this.

    Yohan Farouz

    Hi everyone,
    last year, I presented EZPaperz for syncing Papers with your Android device via Dropbox. Well, this year, let me introduce Aziz, Lite! (https://goo.gl/xy6Uyu), an Android app for synchronizing big libraries from either Mendeley, Zotero or Papers via either Dropbox, Google Drive or OneDrive.

    With Aziz, Lite! we really try to solve unanswered limitations: for many researchers, the 2Gb free space on Mendeley's server is not enough (I, myself, need more than 10Gb). What if you wanted to place your files on another Cloud instead? Well, with Aziz, Lite!, you can choose between Dropbox, Google Drive or OneDrive instead.

    You can download the app directly from here: https://goo.gl/xy6Uyu

    And if you feel like you want to help me promote the app, don't hesitate spreading the word and following the app development on social networks:
    - Twitter: https://goo.gl/YZSbGU
    - Facebook: https://goo.gl/M233fX
    - Google +: https://goo.gl/0WFAAq

    More information and Youtube tutorials on : http://www.azizlite.net/

    Don't hesitate to send me your feedback.
    Cheers,
    Yohan,

  • Yohan Farouz added an answer in Citations:
    12
    Which citation manager do you prefer?
    a) Mendeley
    b) CiteULike
    c) Endnote
    d) Zotero
    e) RefWorks
    f) Any other (please specify)
    Yohan Farouz

    Hi everyone,
    last year, I presented EZPaperz for syncing Papers with your Android device via Dropbox. Well, this year, let me introduce Aziz, Lite! (https://goo.gl/xy6Uyu), an Android app for synchronizing big libraries from either Mendeley, Zotero or Papers via either Dropbox, Google Drive or OneDrive.

    With Aziz, Lite! we really try to solve unanswered limitations: for many researchers, the 2Gb free space on Mendeley's server is not enough (I, myself, need more than 10Gb). What if you wanted to place your files on another Cloud instead? Well, with Aziz, Lite!, you can choose between Dropbox, Google Drive or OneDrive instead.

    You can download the app directly from here: https://goo.gl/xy6Uyu

    And if you feel like you want to help me promote the app, don't hesitate spreading the word and following the app development on social networks:
    - Twitter: https://goo.gl/YZSbGU
    - Facebook: https://goo.gl/M233fX
    - Google +: https://goo.gl/0WFAAq

    More information and Youtube tutorials on : http://www.azizlite.net/

    Don't hesitate to send me your feedback.
    Cheers,
    Yohan,

  • James A Green added an answer in Rock Art:
    27
    How does the study of rock art and archaeology make a difference?

    Are these studies relevant?  Can they contribute anything to our contemporary industrial society?  What types of people would see our academic and conservation efforts to be of value?

    James A Green

    Marcel,

    Je suis d'accord, oui. Lilliana will vouch that sometimes I play the Devil's Advocate. I believe that these discussions not only help to share information and ideas, but also to explore topics in different ways. To expand the mind. To show that there is more than one approach to a problem. More than one view.

    Yes, not doubt early man, possibly even early hominin species, created art. I am sure that artists practiced with whatever medium they work. That is the bane of archaeology/anthropology: the incomplete picture (pun intended). What we find often is only a drop in the bucket to what once was. Imagine a stone thrown far out in a lake: we can only see the ripples that lap the section of shore on which we stand, all else is lost or obscure.

  • Yohan Farouz added an answer in Bibliographical References:
    9
    Which tools are used by researchers for formatting references as per journal criteria? I do this manually and it's too tedious.
    Suggestions are appreciated. I have heard of EndNote, but I have never tried it.
    Yohan Farouz

    Hi everyone,
    last year, I presented EZPaperz for syncing Papers with your Android device via Dropbox. Well, this year, let me introduce Aziz, Lite! (https://goo.gl/xy6Uyu), an Android app for synchronizing big libraries from either Mendeley, Zotero or Papers via either Dropbox, Google Drive or OneDrive.

    With Aziz, Lite! we really try to solve unanswered limitations: for many researchers, the 2Gb free space on Mendeley's server is not enough (I, myself, need more than 10Gb). What if you wanted to place your files on another Cloud instead? Well, with Aziz, Lite!, you can choose between Dropbox, Google Drive or OneDrive instead.

    You can download the app directly from here: https://goo.gl/xy6Uyu

    And if you feel like you want to help me promote the app, don't hesitate spreading the word and following the app development on social networks:
    - Twitter: https://goo.gl/YZSbGU
    - Facebook: https://goo.gl/M233fX
    - Google +: https://goo.gl/0WFAAq

    More information and Youtube tutorials on : http://www.azizlite.net/

    Don't hesitate to send me your feedback.
    Cheers,
    Yohan,

  • Ewan P Plant added an answer in Candida Albicans:
    1
    How can Candida genomic library complement yeast mutants?

    Candida albicans is known to have CUG codon bias for serine, which universally encodes leucine. How is it possible to use Candida library in Saccharomyces mutants for complementation studies considering this codon bias?

    Ewan P Plant

    A lot of proteins will be able to tolerate a amino acid substitution at one position.  There will always be differences in the coding sequences between two different species so there will never be complete overlap.

    Other things that can cause problems with complementation studies include the expression of other genes in the cloned gene fragment that includes the gene you are trying to complement.

  • Anahita Abbasnejad S. added an answer in ARM Microcontroller:
    4
    Is it possible to program an ARM microcontroller by MATLAB to work as an ADC?

    to whom may concern!

    Hi;

    I am working on a project witch I need an 8 channel ADC by ARM microcontroller Xmega xx. As I know nothing about programming an ARM microcontroller, I've decided to program it by MATLAB.

    Is there any way to convert MATLAB code to program this microcontroller? Infact I mean without installing MATLAB itself on the microcontroller to compile the program.

    Anahita Abbasnejad S.

    Hi Jonas!

    Hi U.Dreher!

    Thank you. Your answers were really helpful.

  • Marielsa Gil added an answer in Pseudomonas fluorescens:
    13
    for all member, What is differences of pseudomonas fluorescens and pseudomonas aeruginosa, and how to identify both?

    I got bacteria (may be pseudomonas) from root and then cultivated on king's B medium. What is the bacteria (pseudomonas fluorescens or pseudomonas aeruginosa)?

    Marielsa Gil

    Completely agree with Dr. José Luis however not always available techniques are so expensive at least in my country and therefore a simple way to approach to differentiate in the laboratory is to place a brain heart infusion broth with the inoculate suspect and place in water bath at 42 ° C if no growth in 24 hours microorganism is P. aeruginosa, also in the susceptibility can be attached 1 disc kanamycin and carbenicillin according to one of Koneman et al P. aeruginosa is resistant to kanamycin and sensitive carbenicillin while P. fluorescens is the opposite.

    I hope to help

    Sorry, my english is very bad

  • Raam Kasinathan asked a question in Supercapacitors:
    New
    Can efficiency of e-car(fully electric or hybrid) be increased with super capacitor?

    In electric cars, during the start up we need more torque so more power is needed to push the car from complete rest. As the energy obtained from braking(regenerative braking) is for a very short duration, if we use a supercapacitor to store that short recuperating electric energy and If we use that high power from supercapacitor during starting and sudden acceleration scenarios. Will there be any efficiency increase?  In which software can we simulate and analyse it?

  • Stefano Doglio added an answer in Bone Histology:
    16
    How should I prepare and stain bone to enable the counting of LAG's?
    We're trying to age Alpine tree frogs. We've decalcified in 10% formic acid for 24hrs, then cut 10um sections from the 3rd phalange and stained with meyers haemotoxylin for 1hr. We can see the lines of arrested growth (LAG's) but they are very faint. How could we make the LAG's darker and therefore easier to count?
    thanks!
    Stefano Doglio

    apart from trying with Ehrlich or Boehmer haematoxilin as David Tarkhnishvili wrote I usually also clean the bone before starting with the procedure. In my experience small species are always more difficult...

    Anyway, in this paper of ours: "First skeletochronology results of Bufo (Epidalea) brongersmai from the Moroccan Anti Atlas (Anura, Bufonidae)" we briefly described our protocol, hope this helps :-)

    Addendum: just realized it's a 2 years old thread!

  • Nata Kuzlo asked a question in Grammar:
    New
    Who has information about lexical and grammar skills in reading texts or images?

    I mean connection of skills from linguistic competence with reading.

  • Oswaldo Lopez Santos added an answer in Solar Inverters:
    2
    How can I calculate ripple on output voltage of grid side LC filter for Solar Inverter?

    Hello,

    I have 833 kW solar inverter whose output voltage is 560 V. MPPT range of this inverter is 800-1270 V DC. I have designed LC filter based on theoratical calculation and resonance freq, as 30% of switching freq. (4000 Hz). The simulation of the system shows satisfactory results in terms of THD. Now, I am looking for the optimization of the analytically calculated value.

    However, I am not sure, how the DC input voltage will affect on my AC output voltage of inverter. The voltage ripple will surely increase with increase in input DC voltage from 800 V to 1270 V. I am also aware that the shunt capacitor will have effect on voltage refinement and L will have an effect on current refinement. The question is under:

    1. How can I calculate ripple voltage on generated output voltage waveform by capacitor. Can I do it by merely putting a summation block with inputs before and after filter and watching its value as I increase input DC voltage from 800-1270 V?

    2. How can I optimize C value such that, my LC filter can operate satisfactorily between 800 V- 1270 V DC input? Is there any analytical method or do I have to do it merely by simulation? Is drawing frequency response (bode plot) of LC filter be the solution as an answer of this quesition?

    Your expert advice will be much appreciated.

    Thanks and Regards,

    Aalok

    Oswaldo Lopez Santos

    Dear Aalok.

    If I understood, you have a DC-AC converter with LC filter connected to a load at the AC side. Also, you have this converter connected to a PV array at the DC side.

    Considering the output of the converter as the input of the filter-load system you can model it as a signal with a fundamental component and some relevant harmonic components whose amplitud is function of the DC voltage. On the other hand your filter-load system transfer function varies because of the power load changes. Then you can work with the frequency response (multiple as a function of the power load) and the DC voltage determining the ripple component.

  • Grant Shimamoto added an answer in Dioxygenases:
    8
    How can I determine a cofactor for an inactive enzyme?

     I am having issues restoring activity to an extradiol dioxygenase that I am studying.  It is a recombinant form of a Rhizobium enzyme, expressed with a N-terminal 6xHistag in E. coli BL21 cells.  I suspect that the Histag is somehow responsible for inactivity and I am in the process of cleaving it using thrombin.  

         I can't help but thinking that I am overlooking the fact that a cofactor is required by the enzyme.  The same enzyme has been shown to work using the cell lysate and although my goal is to be using purified protein I can't even get the cell lysate to work.  It is my understanding that LB media is supplemented enough to provide minute amounts of possible cofactors for growth.  Additionally I supplemented the LB media with 1mMFeCl2 (ferrous iron binding site on enzyme) and still no activity.  Previous work on this enzyme involved growing in YEM broth, which I can't imagine making a big difference.

    Would using YEM media over LB make any difference in activity of my enzyme?

    How does one usually determine which cofactor is required for an enzyme?  Besides looking for conserved metal binding domains is it a good idea to create a cofactor "cocktail" and use that to determine activity?

    Thank you for any words of advice.

    Grant Shimamoto

    Do you have purified active enzyme from Rhizobium that can be subjected to ICAP (inductively coupled argon plasma) analysis for metals?  It's extremely sensitive and covers a wide range of elements.  Extraction of a complete protease digestion may also release any bound solutes then try mass spec of the digest extract?

  • Jonas Nyman added an answer in Saccharomyces cerevisiae:
    5
    Is it possible to cultivate S. cerevisiae on fatty acids free media?

    It is possible to cultivate Saccharomyces cerevisiae on medium which is polyunsaturated fatty acids (PUFA) free? Common baker yeast are produced on molasses (full of PUFA) and agar is made of seaweed/red algae (also PUFA).

    Jonas Nyman

    Yes, Dr Pronk is right! The minimal media I mentioned only works in aerobic conditions. I simply assumed that was the case here. 

  • Andrea Piroddi added an answer in SAR:
    1
    Does anyone Know how to mitigate the effect of Foliage on SAR Imaging ?

    SAR image for target camouflaged by trees leads to the fluctuation of the amplitude and phase which in turn effect the sidelobes. therefore, the image of SAR is distorted   

    Andrea Piroddi

    The interest in the use of spaceborne synthetic aperture radar (SAR) for collecting earth bio/geophysical information and detecting foliage-obscured targets has been increased. However, the signals are inevitably affected by the ionosphere, particularly at very high frequency and ultrahigh frequency. Thus, it is crucial to understand the potential effects of the ionosphere on SAR systems.First, for analyzing range resolution, in addition to linear and quadratic phase errors due to the background ionosphere, the cubic phase error has to be considered. Second, for analyzing azimuthal resolution, the effects of ionospheric irregularities must be evaluated under the conditions of oblique incidence and anisotropic irregularities. Finally, based on the theory of moment equation, range resolution degradation caused by the multiple scattering have to be studied in the case of anisotropic irregularities.

    best regards

  • George Stoica added an answer in Social Norms:
    79
    What is your personal religious pluralism ?

    Wikipedia definitions of religious pluralism:

    Religious pluralism is an attitude or policy regarding the diversity of religious belief systems co-existing in society. It can indicate one or more of the following:

    • As the name of the worldview according to which one's religion is not the sole and exclusive source of truth, and thus the acknowledgement that at least some truths and true values exist in other religions.
    • As acceptance of the concept that two or more religions with mutually exclusive truth claims are equally valid. This may be considered a form of either toleration (a concept that arose as a result of the European wars of religion) or moral relativism.
    • The understanding that the exclusive claims of different religions turn out, upon closer examination, to be variations of universal truths that have been taught since time immemorial. This is called Perennialism (based on the concept of philosophia perennis) or Traditionalism.
    • Sometimes as a synonym for ecumenism, i.e., the promotion of some level of unity, co-operation, and improved understanding between different religions or differentdenominations within a single religion.
    • As term for the condition of harmonious co-existence between adherents of different religions or religious denominations.
    • As a social norm and not merely a synonym for religious diversity.

    These are forms of societal religious pluralims but as individual one is free to create his/her own form of personal religious pluralism.  The question's purpose is to gather tertimonies of personal religious pluralism.

    George Stoica

    Live and let live.

  • Michael Delannoy added an answer in Biotechnological Techniques:
    3
    Can I keep Hoechst DNA staining slides for long time?

    I am trying to store my slides carrying stained DNA using fluorescent dye (Hoechst DNA staining) for a long period of time. how can I do it?,...Do you have any advice?

    Michael Delannoy

    I agree with using an anti-fade and sealing the coverslip (nail polish). Keeping the slides in the dark as suggested is advisable and you can also store them at 4C (never frozen) cold room for longer storage.

  • Alfredo Pereira Junior added an answer in Cognitive Systems:
    99+
    Is Chalmers' so-called "hard problem" in consciousness real?

    In his 2014 book "Consciousness and the Brain: Deciphering How the Brain Codes Our Thoughts" Stanislas Dehaene wrote "Chalmers, a philosopher of the University of Arizona, is famous for introducing a distinction between the easy and the hard problems. The easy problem of consciousness, he argues, consists in explaining the many functions of the brain: how do we recognize a face, a word, or a landscape? How do we extract information form the senses and use it to guide our behavior? How do we generate sentences to describe what we feel?

    “Although all these questions are associated with consciousness,” Chalmers argues, “they all concern the objective mechanisms of the cognitive system, and consequently, we have every reason to expect that continued work in cognitive psychology and neuroscience will answer them. By contrast the hard problem is the “question of how physical processes in the brain give rise to subjective experience … the way things feel for the subject. When we see for example, we experience visual sensations, such as that of vivid blue. Or think of the ineffable sound of a distant oboe, the agony of an intense pain, the sparkle of happiness or the meditative quality of a moment lost in thought … It is these phenomena that poses the real mystery of the mind”."

    Stanislas Dehaene's opinion is "that Chalmers swapped the labels: it is the “easy” problem that is hard, while the “hard” problem just seems hard because it engages ill-defined intuitions. Once our intuition is educated by cognitive neuroscience and computer simulations, Chalmers’ “hard problem” will evaporate".

    Personally, I agree with Stanislas Dehaene's opinion.

    Alfredo Pereira Junior

    Dear Arnold, you wrote:

    "The large population of autaptic neurons in retinoid space already has integrated all the features/patterns that you mention, and does so by organizing each feature in proper spatio-temporal register (feature binding) within a global space with a locus of perspectival origin (the core self, I!). I don't see how the astrocyte network can conserve the egocentric spatiotemporal organization -- the distinctive subjectively organized  combination of sensory patterns that give each feeling its special quality -- of the conscious experience, and even if it were able to do so, I don't see what it would add to the experience.""

    Alfredo: It adds the feeling of the episode. I never claimed that the astrocyte network do what neurons already do. Neurons process information, integrate stimuli features, provide spatial location of objects, constructs egocentric space, etc.etc.etc. They just do not feel the meaning of the episode. They do not have a mechanism for feeling the meaning of the information they integrate. Please do not confuse information integration with feeling. Please do not confuse spatial location with feeling. And please do not confuse perception of one's own body in space with feeling.