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  • Joana Bless Perey added an answer in Social Support:
    2
    Does anyone know how to interpret PSS-Fa Scale?

    Perceived Social Support from the Family Scale

    Joana Bless Perey

    Yes, your answer was helpful. Thank you!

  • Aya Kabbara added an answer in Freesurfer:
    4
    Do you have any suggestions for an efficient software to coregister two MRI volumes?

    Hello,

    I have two volumes:  one structural MRI, and a functional NIFtii atlas. And I need to coregister these two volumes. 

    What can be the suitable software to do this task (slicer,  freesurfer)? Slicer is a friendly software with a GUI, but I don't know how accurate it is. 

    What are your suggestions? 

    Aya Kabbara

    Thank you all.

    @ Ali, I heard a lot about FSL software you proposed. I will try it very soon

    @ Mauro, thank you a lot. I tried SPM and it works well for this task.

    @ Salem, thank you!

  • Bhagwat Kedar added an answer in Piping:
    2
    How can I get Acoustic Mode shapes in Ansys? How can I calculate FEA based Fatigue life from ANSYS?

    Hi All

    Pipe Upstream Pressure = 47.5 bar

    Pipe Downstream Pressure = 10.31 bar

    Pipe diameter = 457 mm

    Pipe thickness = 6.35 mm

    please find the Attached file..

    Bhagwat Kedar

    Thank you sir....and How can I get that Mode Shapes?

  • Silas Maujih asked a question in Forced Migration:
    New
    Kindly can i be able to get any document by Colson E,2003. Forced migration and anthropological ?

    Kindly assist me access any material by Colson E on forced migration and Marcus,GE(anthropology through thick and thin - princeton University)?

  • Isam Eldin Hussein Elgailani added an answer in Calculations:
    2
    Hi everyone please can you tell me how to calculate Jacobson's constant for inter-molecular free length Lf?

    I need to calculate intermolecular free length Lf, I know the question but I am not able to find out how to calculate Jacobson's constant. 

    Isam Eldin Hussein Elgailani

    Dear Sir. Concerning your issue about how to calculate Jacobson's constant for inter-molecular free length Lf . You can visit the following link and the file attached which may help you in your analysis:

    http://www.sciencedirect.com/science/article/pii/0041624X79900325

    Thank

  • Angelo Pidatella added an answer in Quantum ESPRESSO:
    1
    How to calculate Hubbard U parameter for different atoms in a given compound?

    I am using Quantum espresso, I would like to know about calculating Hubbard U parameter using ACBN0 scheme for different atoms in a compound. since the selection of correct U and J parameter are necessary to find electronic structure and magnetization values. It will be more helpful if we can generate U values.

    Angelo Pidatella

    Hi,

    probably this tutorial may be helpful in your case.

    http://hjklol.mit.edu/content/calculating-hubbard-u

    Best,

    A.

  • Aymen Bourezgui added an answer in Spin Coating:
    2
    How can I explain why the thickness of porous TiO2 films is three times smaller than the grain size of anatase?

    TiO2 porous films were deposited on ITO substrates by spin coating technique.  I have found the thickness of porous TiO2 films is three times smaller than grain size of anatase. How can I explain this phenomenon?

    Aymen Bourezgui

    Thank you  DR Tobias Deutschmann

    I used AFM to get thickness . Films were heat treated at 550°C for 2 hours

  • Daniel Ejebe added an answer in Rabbit:
    2
    What is the most effective way to avoid interference from the heavy chain of IP antibody in the IP:WB?

    I am thinking that choosing the IP antibody from a specie different from that used as primary and secondary antibodies during the IP western blot is the way to go i.e  mice IP ab/rabbit primary ab/ anti rabbit secondary ab or rabbit IP/mouse primary/ anti mouse secondary.

    Daniel Ejebe

    Thanks Jordan, Option 2 will be looked into as I am already using 0.1M Glycine pH 2 for elution.The question then arises if the heavy and light chains of the IP antibody have their epitops denatured beyond recognition why do they still show up on the blot? How does the secondary 'recognize' them?

  • Behrouz Ahmadi-Nedushan added an answer in Educational Systems:
    63
    How should / could we improve the Educational System, to adapt modern times, and future generations?

    Most would agree that Education plays a fundamental role in solving many of the humanity's current problems.

    I believe in a better World.

    Mass education for all youngster can play a good role in the near future.

    Should we change our system?

    Should we act on University level?

    Should we act transversally from kindergarten, through basic education?

    I should like your valuable contributes.

  • Aslanbek Naziev added an answer in Equality:
    2
    What is the definition of a quadrilateral?

    In the glossary at the end of the attached paper, I define a quadrilateral as:

    Quadrilateral              The union of two triangles adjacent on their longest sides

    1.  Kite                         The two triangles are congruent and uncommon sides that are equal are also consecutive

    2.  Parallelogram        The two triangles are congruent and uncommon sides that are equal are also opposite

    3.  Rectangle               A quadrilateral with all equal angles

    4.  Rhombus                A quadrilateral with all equal sides; plural, rhombi

    5.  Square                    A quadrilateral with all equal angles and sides

    I do not define the term trapezoid but instead define the triangle frustum:

    Frustum                      

    1.  Triangle                              The part of a triangle between the base and a parallel segment called the top

    2.  Circle                                  The part of a circle between it and a chord

    Later, when I introduce solid geometry, frustum will be naturally extended to solids whose tops have been cut off with a plane parallel to the base.  Observe that the term "segment" does not mean what I call a circle frustum.  It has only one meaning:

    Segment                     All the points along the shortest path between two points

    Do you know of a better way to define a quadrilateral?  If so, why do you feel that it is better?

    • Source
      [Show abstract] [Hide abstract]
      ABSTRACT: I propose to write a textbook on the subject of Euclidean geometry. It will be divided into two volumes, Geometry without Multiplication and Geometry with Multiplication. In the future I may write a third volume about solid geometry. Geometry without Multiplication ranks high school geometry students as follows: White Belt: Abstract Algebra Orange Belt: Triangle Congruence Green Belt: Quadrilaterals Red Belt: Quadrature Black Belt: Circles I have written the text for white- and orange-belt students and I have written an outline of the remaining textbook. I will update as I progress. I hope to receive comments on my proofs and on my organization of theorems from other geometers during the writing of this book. Why the martial arts analogy? We are under attack from the math haters. Asad Zaman writes: “It is a completely mistaken idea that scientific theory is based on deductions from a series of postulates – that is the description of the methodology of mathematics… There is no science which uses axioms and logical deductions to derive scientific theory. “Mathematics is not a ‘science’ since it is not based in any direct way on observational evidence. Unlike scientific laws, mathematical laws are not affirmed observational evidence. Recognition of the possibility that there are bodies of knowledge which are not science would lead to greater tolerance and pluralism which is currently desperately needed.” Tolerance? No. Such outrageous talk by the World Economics Association has brought war and discord to our once quiet study of triangles. If Asad Zaman were just some crazy man, it would not matter. But Zaman is the editor of the Real World Economics Review. He wields this terrifying power to no other end than to ban all mention of deductive logic. I alone stand in his path. If I falter, then geometry will be excluded from the high school curriculum and a whole generation will be taught only hate for deductive logic. Can you help me fight the Islamic extremists? Intimidating their enemies by cutting off heads and baffling them by slyly redefining dialectical materialism as “ontology” and historical materialism as “processual,” the Islamists and the Marxists have united to rid the world of geometry and to raise a generation of Westerners with only hate for deductive logic. Logic alone can defeat the haters!
      Full-text · Article · Jan 2016
    Aslanbek Naziev

    Yes, George!

  • Syed A Ali added an answer in Site-Directed Mutagenesis:
    3
    How do i solve my problem if there is no expression of protein after site directed mutagenesis experiments?

    I have done site directed mutagenesis in a 4kb plasmid(pQE31) having gene(500bp) encoding 18kDa protein  using Quick change lightning site directed mutagenesis kit from Agilent. Transformed the PCR product into XL gold competent cells. Everything worked fine, after that i sequenced my DNA and confirmed the mutation at 52nd amino acid position. When I transformed the DNA into my expression strain M15 E coli cells there was no expression of the 18kDa protein. my wild type protein was also expressed in the same cells using 0.4mM IPTG. I have tried different concentrations of IPTG and low temperature. Also I confirmed no other insertion of stop codons within the sequence. Also there is no change in the promoter and other sequences. what may be the problem?

    Syed A Ali

    Hello Jeeshma,

    I assume you confirmed the desired mutation by getting your gene of interest (GOI) sequenced. Most likely you did not get sequenced the promoter/operator region upstream your GOI. It is not impossible to get undesired secondary mutations in the plasmid vector that contains your GOI. Since you have already got at-least one clone with desired mutation, simply use it to replace the WT gene in the plasmid vector that is correctly expressing the WT gene. You can do it by excising out the WT (and mutated) gene using appropriated restrictin enzymes and subsequent ligation. Good luck!

  • Vimalraj Padayatchiar Govindan added an answer in Dogs:
    9
    Which method is suitable for spaying in dogs? Right flank approach or Mid Ventral approach?

    The best way to do spaying in dogs especially in stray dogs either by right flank or Mid-ventral???

    Vimalraj Padayatchiar Govindan

    Dr. Mehrajuddin Naikoo: Excellent, Have you tried Rt. Flank. If not try for consecutive 3 times and pls share your experience.

  • Rachna Chandra asked a question in Flora:
    New
    I want to know about the natural flora of Kolar Gold Fields i.e. before the mines were systematically explored.

    I intend to gather information about the plants which existed before the KGFs were mined systematically. 

  • Manuel Ortega-Calvo added an answer in Primary Care:
    10
    In your opinion who are the nurses with more academic and research influence on the English primary care from 1940 to the present?

    I am preparing a review of the subject and need information.

    Manuel Ortega-Calvo

    Thanks for all the replies.

  • Debabrata Sengupta asked a question in Fuel Cells:
    New
    What will be the minimum criteria for using a molecule in direct alcohol fuel cell (DAFC)?

    What will be the minimum criteria (like performing some definite chemical reaction) for using a molecule in DAFCs? Please consider Grutzmacher complex.

  • Hazim Hashim Tahir added an answer in Political Socialization:
    14
    Who is a sycophant? Is sycophancy an Art?

    We see sycophants in all the fields - political, social, administrative ......

    Do you think it is an art that is harmless or something that harms the organisational culture severely.   

    What kind of sycophants are prevalent in your part of the world.

    Hazim Hashim Tahir

    Dear Colleagues,

    Good Day,

    "How can he [today's writer] be honored, when he does not honor himself; when he loses himself in the crowd; when he is no longer the lawgiver, but the sycophant, ducking to the giddy opinion of a reckless public."

                                                         -----  Ralph Waldo Emerson

  • Adil H.H. Bashir added an answer in Diabetes Complications:
    13
    The types of complications associated with diabetes differs between individuals. What are the reasons?

    Regarding the complications associated with diabetes, what is the less understood consequence of prolonged hyperglycemia acting differently in different individuals?

    Adil H.H. Bashir

    Dear Ali Merina Houria , hope you find these references of fulfilling your interest.

     Bashir, A.H.H. (2004). Clinico-epidemiological Study of Cutaneous Manifestations of Diabetes mellitus in Jabir Abu Eliz Diabetic Center in Khartoum, Sudan. Paper, Collage of Medicine, University of Juba, Sudan- Sudanese Journal of Dermatology. September 2004; 2(2):34-38.

    1. National Diabetes Data Group 1979.Classification and diagnosis of diabetes mellitus and other categories of glucose intolerance. Diabetes; 28:1039-57.

    2.Perez M I, Kohn SR 1994.Cutaneous manifestations of diabetes mellitus. J Am Acad.Dermatol; 30:519-31.

    3.OyerDS 1982.Diabetes mellitus: new developments. Arch.

    Dermatol.:118:132-4.

    4. Diabetes Statistics-MSN Home/Hotmail-For the latest MSNBC Health. Internet.

    5. Sonck CE, Somersalo O 1963: The yeast flora of the anogenital region in diabetic girls. Arch. Dermatol. 88:846-852.

    6. Knight L, Fletcher J 1971: Growth of Candida albicans in saliva: Stimulation by glucose associated with antibiotics, corticosteroid, and diabetes mellitus. J Infect Dis 123:371-377.  

    7. Lugo-Somolinos A, Sanchez JL 1992: Prevalence of dermatophytosis in patients with diabetes: J. Am. Acad. Dermatol. 26:408-410.

    8.Alteras I, Saryt E 1979: Prevalence of pathogenic fungi in the toe-webs and toe-nails of diabetic patients. Mycopathologia 67:157-159,.

    9. Cole GW, Headley J, Skowsky R (1983): Scleredema Diabeticorum: A common and distinct cutaneous manifestation of diabetes mellitus. Diabetes Care 6:189-192.

    10. West KM 1978: Epidemiology of diabetes and its vascular lesions. New York, Elsevier North-Holland Inc. p 353.

    11.Huntley AC, Walter RM Jr 1990: Quantitative determination of skin thickness in diabetes mellitus: relationship to disease parameters. Journal of Medicine, , 21(5):257-64.

    12. Blackwell science Ltd. Rook/Wilkinson/Ebling 1998. Textbook of dermatology. Sixth edition.

    13. Gupta AK, Humke S. The prevalence and management of onychmycosis in diabetic patients. University of Toronto, Toronto, Canada.

    14. Murphy RA 1965: Skin lesions in diabetic patients: The “spotted leg” syndrome. Lahey Clin Found Bull 14:10-14.

    15.Bear CL, Wall LN 1969. Idiopathic bullae in diabetics. Australas J Dermatol; 10: 337.

    16. Derighetti M, Hohl D, Krayenbuhl BH, Panizzon RG. Bullosis diabeticorum in a newly discovered type 2 diabetes mellitus. Department of Dermatology, DHURDV, CHUV, Lausanne, Switzerland.

    17. A Huntley December 1995. Photo-essay: The Skin and Diabetes Mellitus. Dermatology Online Journal, Volume 1, Number 2.

    18.Boyd-AS; Neldner-KH 1991 Oct. Lichen planus, Department of dermatology, Texas Tech University Health Sciences Centre, Lubbock 79430. J-AM-Acad-Dermayol.; 25(4) :593-619.

    19. Karvonen SL, Haapasaari KM, Kallioinen M, Oikarinen A, Hassinen IE, Majamaa K 1999 Apr. Increased prevalence of vitiligo, but no evidence of premature ageing, in the skin of patients with bp 3243 mutation in mitochondrial DNA in themitochondrial, encephalomyopathy, lactic acidosis and stroke-like episodes syndrome (MELAS). Br J Dermatol; 140(4): 634-9.

  • Sophia Varghese added an answer in Powders:
    3
    Why Intensity of XRD peak increases by milling powders in dry medium?

    Generally after milling of crystalline material, decrease in intensity of the XRD peak is  observed indicating decrease in crystallinity, due to milling for longtime. At higher rpm, peak narrowing and increase in intensity is observed, what can be the reason behind it.

    Sophia Varghese

    Thank you Alexandre and R. Taherzadeh...It would be helpful if you can throw some light  on,  how to identify the preferential plane exposure during milling?

  • Cheryl Withaneachi added an answer in Higher Education:
    9
    How has your university changed as free market principles are more apparent in your institutions?

    As neo-liberalisme policies are becoming a prominent feature in Higher education, I'm interested to see the perception of students and staff. in regards to its impact [ in terms of curriculum, teaching & learning, research [quantity vs quality] etc]

    Cheryl Withaneachi

    Thanks guys for all the contributions. Here in Malaysia we see familiar patterns that is worrying....

  • Morteza Shokri-Ghasabeh added an answer in Construction Project Management:
    2
    Which aspect / application of PMO in construction industry requires further research?

    I am doing Masters in Construction Project Management from University of Liverpool. I am now searching for an appropriate issue for Dissertation research relating to PMO in the construction industry.

    Morteza Shokri-Ghasabeh

    Hi Syed Ehtesham Husain,

    My suggestions would be related to organisational level:

    1. Challenges in/Barriers to implementing an effective PMOs in Construction Industry - You can undertake a good and thorough literature review and use questionnaire and possibly interview to deliver your dissertation
    2. PMO: What are the benefits? Theory vs Reality - you may use interview and questionnaire as your tools.

    Good luck Syed Ehtesham Husain. :)

  • Isam Eldin Hussein Elgailani added an answer in Acetonitriles:
    7
    Is there any way to measure the pH of an acidified Acetonitrile solution?

    I wanna do some pH dependence study of absorption and fluorescence spectra.

    Isam Eldin Hussein Elgailani

    Dear Sir. Concerning your issue for the way to measure the pH of an acidified acetonitrile solution in absorption and fluorescence spectra you can visit the following link and the file attached which may help you in your analysis:

    https://www.academia.edu/18339624/Solvent_and_pH_Dependent_Fluorescent_Properties_of_a_Dimethylaminostyryl_Borondipyrromethene_Dye_in_Solution

    Thank

  • Syed Naveed Akhtar asked a question in AoA:
    New
    AOA, I want to prepare a research paper about theory of Computation, please tell me related topics on which i shall do a fruitful research?

    Please tell me topic related to "Theory of computation" on which i make a research to complete my research paper?

  • Nicola Viale added an answer in Finite Element Analysis:
    8
    Can someone help me for the propagation of a cohesive crack in franc2d?

    I've some problem with franc2d 3.1. I want to propagate a cohesive crack, i defined the material properties and the properties of the non linear interface. when i want to propagate the fracture the program ask me the number of interaction and the precision of the solution. I insert this values and after that the program collapse.

    what i can do?
    there are some other open source software for model cohesive crack?

    Forgive me for my english.

    Nicola Viale

    I have defined the load, the crack and the fixity in the file m2.wdb. I tried to dowload  and open it and these conditions were there. it can be some problem with the version of sowthare? i'm working with v3.0
    thank

  • Rachna Chandra added an answer in Statistical Testing:
    5
    What statistical tests we can conduct to understand the differences in geochemical processes among sites?

    Which all statistical tests should be carried out for interpreting geochemical data set to find differences among sites, seasons, metal species, ionic bonding, etc.

    Rachna Chandra

    @Donald A Singer: Thanks for your kind reply.

  • Debabrata Sengupta asked a question in Galactose Oxidase:
    New
    What is the rate equation for galactose oxidase?

    Please let me know the rate equation for galactose oxidase. If two molecules are attached in TS. Is it possible that rate is dependent on both the molecules?

  • Adil H.H. Bashir added an answer in Schoenlein-Henoch Purpura:
    2
    Do you consider Extra digestive Helicobacter pylori skin mafestations, EdHpSm as maker to gastric disease condition?

    Helicobacter pylori are gram-negative; microaerophilic spiral rod-shaped bacteria and they lead to gastritis, duodenal or gastric ulcer and even in rare cases to gastric carcinoma or Mucosa Associated Lymphoid Tissue (MALT) lymphoma. Based on a number of reports, a possible relationship of H. pylori infection to a variety of different dermatosis has been suggested, including urticaria, rosacea, acnerosacea, atopic dermatitis, alopecia areata, Sjögren’s syndrome, Schönlein-Henoch purpura, and Sweet’s syndrome.

    Adil H.H. Bashir

    Dear Dvoryanchikovو Thanks for sharing discussion as a lot of controversy is raising as regards H. pylori pathogenicity and the the question of; is a commensal cause Adenocarcinoma and MALT lymphoma which is proved by WHO as 6 fold carcinogenic to guard against GERD?! Helicobacter pylori are gram-negative, microaerophilic spiral rod-shaped bacteria that live just beneath the antral
    gastric mucous layer, on the surface of epithelial cells. Stomach infection with this organism causes inflammation of the gastric mucosa, which can lead to gastritis,
    duodenal or gastric ulcer and even in rare cases to gastric carcinoma or Mucosa Associated Lymphoid Tissue (MALT) lymphoma (Covacci et al., 1999). Approximately 50% of the world's population is believed to be infected with H. pylori. Most infections are probably acquired in childhood (Czesnikiewicz-Guzik et al., 2004), but geographic locale, age, race, socioeconomic status, and hygiene seem to play roles in the prevalence of H. pylori. Most of the informations about H. pylori infection rates come from seroprevalence studies. Higher rates of
    infection tend to occur at a younger age in developing countries compared to developed countries and in regions characterized by lower socioeconomic status and higher density living.1 Looking at racial differences in the US, it is found that whites of non-Hispanic origin have lower prevalence of infection compared to African- Americans or Hispanics (Brown, 2000). It has been speclated that dental plaque might harbor H. pylori and, therefore, might be a source of gastric infection
    (Czesnikiewicz-Guzik et al., 2004). Aside from iatrogenic transmission of H. pylori via endoscopy, no definite modes of transmission for H. pylori have been identified.
    However, association studies suggest that there are three other potential routes for the transmission of H. pylori: person-to-person transmission (example, oral-oral or
    fecal-oral), waterborne transmission (example, contaminated water), zoonotic (example, cats and other pets and animals) or vector borne transmission (example, flies). Person-to-person transmission is considered to be the most likely route of transmission considering that isolation of H. pylori from non-human reservoirs has been inconsistent (Brown, 2000). Chronic urticaria (CU) and
    concurrent angioedema are frustrating problems for both physicians and patients (Wedi et al., 1998). Based on a number of reports, a possible relationship of H. pylori infection to a variety of different dermatosis has been suggested, including urticaria, rosacea, acne-rosacea, atopic dermatitis, alopecia areata, Sjögren’s syndrome, Schönlein-Henoch purpura, and Sweet’s syndrome. Larger case-control studies, however, do not confirm this relationship. Therefore, H. pylori eradication therapy cannot be generally recommended in this dermatosis
    (Boni et al., 2000). Pathogenic strains of H. pylori have been shown to activate the epidermal growth factor receptor (EGFR), a membrane protein with a tyrosine kinase domain. Activation of the EGFR by H. pylori is associated with altered signal transduction and gene expression in host epithelial cells that may contribute to pathogenesis (Tomb et al.,1997).

    Auriemma L, Signorelli S (2001). The role of tetracycline in the
    retreatment after Helicobacter pylori eradication failure. Minerva
    Med., 92(3): 145-149.
    Boni R, Burg G, Wirth HP (2000). Helicobacter pylori and skin
    diseases--a (still) intact myth? Schweiz. Med. Wochenschr.,
    130(37): 1305-1308.
    Brown LM (2000). Helicobacter pylori: epidemiology and routes of
    transmission. Epidemiol. Rev., 22: 283-297.
    Covacci A, Telford, JL, Del Gludice G, Parsonnet J, Rappuoli R
    (1999). Helicobacter pylori virulence and genetic geography.
    Science, 284: 1328–1333.
    Czesnikiewicz-Guzik M, Karczewska E, Bielanski W, Guzik TJ,
    Kapera P, Targosz A, Konturek SJ, Loster B (2004). Association of
    the presence the Helicobacter pylori in the oral cavity and in the
    stomach. J. Physiol. Pharmacol., 55 Suppl 2: 105-15.
    Ivashkin VT, Lapina TL, Bondarenko OY, Sklanskaya OA, Grigoriev
    PY, Vasiliev YV, Yakovenko EP, Gulyaev PV, Fedchenko VI
    (2002). Azithromycin in a triple therapy for H. pylori eradication in
    active duodenal ulcer. World J. Gastroenterol., 8(5): 879-882.
    Tomb JF, White O, Kerlavage AR, Clayton RA, Sutton GG,
    Fleischmann RD, Ketchum KA, Klenk HP, Gill S, Dougherty BA,
    Nelson K, Quackenbush J, Zhou LX, Kirkness EF, Peterson S,
    Loftus B, Richardson D, Dodson R, Khalak HG, Glodek A, Vaira D,
    Holton J, Menegatti M, Ricci C, Landi F, Ali A, Gatta L, Acciardi C,
    Farinelli S, Crosatti M, Berardi S, Miglioli M (1999). New
    immunological assays for the diagnosis of Helicobacter pylori
    infection. Gut., 45 Suppl. 1: I23-127.
    Wedi B, Wagner S, Werfel T, Manns MP, Kapp A (1998). Prevalence
    of Helicobacter pylori-associated gastritis in chronic urticaria. Int.
    Arch. Allergy Immunol., 116(4): 288-294.
    Wieczorek D, Raap U, Liekenbrocker T, Kapp A, Wedi B (2004).
    Chronic urticaria in childhood. Hautarzt., 55(4): 357-360.

     Bashir, A.H.H. Clinico-epidemiological study in Sudanese patients, Prevalence and effect of eradicative triple therapy on extra digestive Helicobacter pylori skin manifestations, EdHpSm. Clinical Reviews and Opinions Vol. 3(2), pp. 14-19, February 2011.

  • Debaprasad Koner added an answer in Molecular Biology:
    1
    How do I extract cytosolic and nuclear proteins in their native state?

    I need to extract cytosolic and nuclear proteins in their native state (without using any denaturing or reducing agents). I'm using the following extraction buffer: 20 mM Tris-HCl pH 8.0, 2 % NP-40, 1 mM EDTA, and 1 mM PMSF (probably a protease inhibitor cocktail instead of PMSF) to get the cytosolic proteins. However, since NP-40 doesn't solubilize the nuclear membrane, I need an addition to my protocol to get the nuclear proteins. The caveat is that if I use RIPA for example, the detergent it contains is ionic and will denature the nuclear proteins. Any suggestions what I can use to get the nuclear proteins in their native state?

    Debaprasad Koner

    Dear Zivanovic, 

    Kindly go through the attached article. Hope it will help.

  • ALEX IHEANYICHUKWU Opara asked a question in Contaminant Transport:
    New
    Can someone help me with a simple methodology for modelling of possible leachate contamination from a dumpsite?

    I am currently working on possible contamination of a shallow unconfined aquifer from a municipal solid waste dumpsite. I am using both geophysical and geochemical data and I hope to model leechate contamination transport using Mud path /mud flow software.

  • Aslanbek Naziev added an answer in Elementary logic:
    1
    Look at the following table. Is it true that for every unit x and every unit y there exists a zero lying between x and y?

    1  0  1

    0  0  0

    1  0  1

    Hint: This is not a problem from matrix algebra or, moreover, matrix analysis. This is a problem from elementary logic! 

    Aslanbek Naziev

    For more than 1 day this question collected only 2 views. Maybe the answer is absolutely evident for all, but my practice tells me that many people give to this question wrong answer, namely, the "evident" one! That was the reason for asking this question.

  • Andrey A. Kovalchuk added an answer in Copepods:
    9
    Can anybody identify this seawater benthic copepod?

    Sorry for the low photography quality, definitely a benthic harpacticoid, but I can't tell the genre. 

    If anybody could suggest bibliography or identification keys for benthic copepods as well, it would be great!

    Thanks,

    Guido

    Andrey A. Kovalchuk

    Hi, Guido,

    some keys for the Black and Azov  Sea, but note that both have low salinity !

    + 2 more attachments