U. B. Singh's research while affiliated with Indian Veterinary Research Institute and other places
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Publications (15)
A new process of removing saponins from Mahua seed cake, a commonly available tree of genusBassia, has been developed. The processed cake when added to the rations of calves to the extent of 50% digestible crude protein replacement, had no adverse effect on growth rate of cross-bred dairy calves.
Volatile fatty acid (VFA) production rates were measured by isotope dilution technique in the rumen of buffalo calves fed on wheat straw plus concentrate, green maize, cow pea and berseem. Correlations derived between the VFA production rates and their concentration and DOM were significant except in animals fed on cow pea. The VFA production rates...
Total microbial protein synthesis rates in the rumen of buffaloes were estimated by isotope dilution technique, using 131I-albumin treated with tannic acid as a marker. The animals were fed groundnut cake treated with formaldehyde to meet 50% of their digestible crude protein (DCP) requirement and 2.5% urea molasses mixture was given to meet the re...
1. The production rates of bacteria in the rumen of buffalo ( Bos bubalis ) calves were estimated using an isotope-dilution technique. A series of fifteen experiments was done with animals given green maize and nine experiments with animals given cowpea ( Vigna unguiculata ).
2. The turnover time ranged from 205 to 567 min in the group given green...
The bacteria production rates in the rumen have been estimated by injecting 14C- and 35S-labelled mixed rumen bacteria, either live or killed by treatment with formaldehyde, into the rumen and applying isotope dilution technique. The rate of bacteria production when estimated by using either live- or dead-(protected-)labelled bacterial cells were c...
The rate of production of bacteria in the rumen of buffalo calves kept on two rations was measured using ¹⁴ C labelled Streptococcus bovis and ³⁵ S whole ruminal bacterial cells. The animals received daily either 15–20 kg green maize or 25–30 kg green cow pea in 12 equal amounts at 2-h intervals. The bacterial cells from the rumen of animals mainta...
The rates of production of protozoa in the rumen of buffalo calves have been estimated using a single-injection isotope dilution technique. The calves were fed at 2 levels of crude protein, namely, 13% lower and 19% higher than that recommended by the National Research Council. The animals were given their rations at 2-h intervals for 3 weeks. Ther...
A technique is described for the in vivo estimation of the rate of production of bacteria in the rumen of buffalo calves. The animals were given their daily ration in 12 equal amounts at 2-h intervals. The bacterial cells from the rumen were labelled either with 14C or 36S by in vitro incubation in the presence of [U-14C]DL-leucine or 35S-sodium su...
The production rates of bacteria have been measured in the rumen of zebu calves and buffalo calves. The animals were fed green oat continuously at 2 h intervals. [35S]sodium sulphate was fed to the animals for 5 days at 2 h intervals by mixing in the feed. On the sixth day the radioactive feed was stopped and replaced by non-radioactive feed. The d...
Experiments were conducted on 12 male growing calves of zebu cattle (Bos indicus) and buffalo (Bos bubalis) to study the effect of feeding wheat straw presoaked with water on the voluntary feed intake, digestibility coefficients of nutrients and volatile fatty acid concentration and production. The feed intake was improved significantly (P < 0·05)...
Citations
... Production rate of bacteria and protozoa : Bacterial production rate (BPR) and protozoal production rate (PPR) were determined using isotope dilution technique with 35 S (sodium sulfate) as bacterial marker (Singh et al., 1974) and 14 C (choline methyl chloride) as a protozoal marker (Leng, 1982). ...
... The compositions of the diets estimated in quadruplicates are presented in Table 1. After an adaptation period of 15 days on the respective diets, the total ration was divided into 12 equal parts and fed at 2 hourly intervals starting from 9:00 h and water was given 6 times a day for 5 days to attain a steady state condition in rumen environment (Chaturvedi et al., 1973). The steers were housed in a concrete floored, well ventilated and protected cattle shed in a single row individually in stalls. ...
... Standard of known concentration (50 nano moles/ml) of each AA was run simultaneously to estimate the concentration of the corresponding AA in the test samples (Roach and Gehrke, 1970). Production rate of bacteria and protozoa : Bacterial production rate (BPR) and protozoal production rate (PPR) were determined using isotope dilution technique with 35 S (sodium sulfate) as bacterial marker (Singh et al., 1974) and 14 C (choline methyl chloride) as a protozoal marker (Leng, 1982). i) Preparation of labeled bacteria and protozoa @BULLET Bacteria : Bacterial suspension was prepared in vitro using 150 ml of freshly drawn strained rumen liquor (SRL) incubated with 500 µCi of 35 S sodium sulfate for 12 h at 39°C under CO 2 . ...
... McNaught et al. (1950McNaught et al. ( , 1954 found in their in vivo experiments that the microbial preparations which contained protozoal protein had a higher nutritive value than bacterial protein. The digestibilities of bacteria and protozoa were 74 and 87 %. Verma and Singh (1977) determined the digestibility of the protein of mixed labelled whole rumen bacterial cells in buffaloes and in goats. The radioactivity excreted in the faeces was measured. ...
... Studies where methane emissions were calculated from VFA concentrations (e.g. Chaturvedi et al., 1973;Montoya et al., 2011) were not included. At the end of data collection, 412 papers had been added to the database. ...
... At 0730 h on the third day of abomasal sampling a dose of s X CrEDTA (.5 mCi/steer in 100 ml) was injected into six sites within the rumen. Samples of rumen liquid (110 ml each) were taken by suction from the ventral sac on nine occasions during a 24-h period after S lCrEDTA injection for determination of the fractional outflow rate of 3sS-labeled rumen microbes (Singh et al., 1973) and s 1CrEDTA ' the marker of the liquid phase. ...
... Mahua cake: Mahua seed cake (MSC) is poor in essential amino acids arginine and tryptophan . Varma and Singh (1979) treated MSC in water (1:4) and stirred repeatedly till separation of foam and appearance of oily and yellowish foam. On supplementing the above cake to meet 50% of total DCP requirement of cross-bred dairy calves they observed no adverse effect on their growth rate. ...
... Here, we considered one of the important host physiological effects, feed intake, on rumen microbial ARGs. It is known that feed intake affects microbial growth due to the differential amounts of available substrates for the growth of microbiota [39] and the varied rumen passage rate [40][41][42]. However, our results showed that both the ruminal bacteriome and resistome did not co-vary with host feed intake. ...
... In "in vitro" cultures sequestration was demonstrated in bags of feed, suspended in a liquid medium (Weller and Pilgrim, 1974;Nakamura and Kurihara, 1978;Czerkawski and Breckenridge, 1979). From the number of Demeyer (1976) References: 1, Hogan (1964); 2, Hyden (1961); 3, Warner and Stacy (1968); 4, Ulbrich and Scholz (1966); 5, Pilgrim et al. (1970); 6, Mathison and Milligan (1971); 7, Nolan and Leng (1972); 8, Singh et al. (1973); 9, Singh et al. (1974a); 10, Singh et al. (1974b). ...
Reference: Rumen microbes and digestion of cell-wall
