Svetlana Gonta's research while affiliated with University of Latvia and other places

Publications (15)

Article
Full-text available
Homopolymer PHB and copolymer PHB/HV containing granules were isolated from Azotobacter chroococcum cells and used for latexes formation. Composite latex films were formed from the PHB and PHB/HV latexes with different content of poly (vinyl alcohol) and glycerol and a hydrophobic fluorescent benzanthrone derivated dye 3-piperidinobenzanthrone, pos...
Article
Objectives: Albumin is one of the most generously represented proteins in human blood plasma. Therefore it is important to follow and assess the transport function of albumin in clinic researches. Disturbances in structural/functional properties of albumin play an important role in the pathogenesis of various diseases (e.g. diabetes mellitus). Chan...
Chapter
The effect of different concentrations of iron on the Azotobacter chroococcum growth and PHB accumulation as well as on the characteristics of the isolated native PHB granules and the purified polymer was investigated under conditions of fed-batch fermentations. A new hydrophobic fluorescent P8 was used to study an effect of iron concentration on t...
Article
Full-text available
Objectives: Diabetes mellitus has been one of the most crippling diseases that man has seen, and its prevalence has risen dramatically over the past two decades. Currently, there are over 150 million diabetics worldwide and this number is likely to increase to 300 million or more by the year 2025. Diabetes mellitus increases the risk of many disord...
Article
The effect of aeration level and iron concentration on Azotobacter chroococcum 23 growth, PHB accumulation and antioxidative enzyme activities was investigated in shake flask experiments. Biomass yield and carbon source conversation coefficients increased in the presence of iron in the growth medium and under decreased aeration. The highest biomass...
Article
The intracellular level of free inorganic orthophosphate (Pi) in yeast cells generally depends on the Pi uptake capacity, energy state of the cells in respect to the activity of the membrane-associated ATPases and on the activity of metabolic pathways involved in the production of glycerol and trehalose. Batch fermentation was performed to investig...
Article
Three strains were isolated from hydrocarbon-polluted alpine habitats and were representatives of Cryptococcus terreus (strain PB4) and Rhodotorula creatinivora (strains PB7, PB12). All three strains synthesized and accumulated glycogen (both acid- and alkali-soluble) and trehalose during growth in complex medium containing glucose as carbon source...
Article
This paper presents a quantum chemical calculation of native (2–7 fructoside residues) and chemically modified (2–4 fructoside residues) levan molecule models. A levan modification was carried out by oxidation and the following reduction or hyrdazonation of the fructoside rings. The conformational particularity and reaction ability was studied for...
Article
The Zymomonas mobilis 113 S strain was cultivated in a bioreactor with a working volume of 1.4 l at different stirring regimes in a 15% initial sucrose medium. The levan obtained in the fermentation process was analyzed by gel filtration. Because the sucrose/biomass ratio in the fermentation broth decreased to below 300 g/g, the insufficient concen...
Article
The attachment, growth and product synthesis of non-flocculating Zymomonas mobilis cell, fixed in stainless steel wire spheres (WS), were investigated. The carrier surface was activated by treatment with titanium (IV) chloride (TiCl4) and -aminopropyltriethoxysilane (AS) in an attempt to raise the efficiency in the immobilization of the cells. Syst...
Article
Zymomonas mobilis strain 113 “S” produces levan – an extracellular, viscous, biologically active, non-toxic fructose polymer with a unique structure and extraordinary properties. This polysaccharide was isolated at two different degrees of purity by alcohol precipitation from aqueous solutions and was characterized with respect to some rheological...
Article
Full-text available
An original biologically active bacterial fructan levan, synthesised by a special strain of Zymomonas mobilis, has been isolated and purified from proteins and toxins, and it meets the requirements of medicine [1]. Only limited studies have been made up to present on the structure of levan. We reported previously on the activation of macroorganism...

Citations

... From the common group, we distributed clean-up workers with paroxysmal epilepsy [21][22][23], after myocardial infarction [6,7,23], and with Type 2 diabetes mellitus [24][25][26]. ...
... Since 1994, data have been included in the Latvian State Register of Persons with Occupational Diseases and Persons Exposed to Radiation due to the CNPP Accident maintained by the specialists of the Centre of Occupational and Radiological Medicine (Pauls Stradiòð Clinical University Hospital). On the basis of information collected in the Centre four doctoral theses (Kurjane, 2001;Zvagule, 2004;Reste, 2013;Silova, 2015) and a lot of scientific studies were completed (Bruvere et al., 1995;Churbakova et al., 1996;Eglite et al., 1997;2009;Mironova et al., 1998;Kalnina et al., 2001;2012;2013;2013a;Kumerova et al., 2000;Kurjâne and Soèòevs, 1999;Kurjane et al., 1999;2000;Mazarevica et al., 2000;Mironova-Ulmane et al., 2001;2001a;Petukhov et al., 2011;Reste et al., 2012;2014;Skesters et al., 2005;2006a;2006b;2008;2010;2010a;Viel et al., 1997;Zvagule et al., 2002;. Clinical observations and epidemiological studies among Chernobyl recovery workers showed that these persons suffer from a broad spectrum of different kinds of diseases more seriously than in the non-exposed general Latvian population. ...
... Possible implementations include, inter alia, compostable packaging [7][8][9], agricultural applications [10], medical and surgical devices [11][12][13], or biolatexes [14,15]. The composition of PHA on the molecular level determines their properties and is fixed in statu nascendi during biosynthesis. ...
... no gas phase in liquid) with Z. mobilis, agitation intensity has been found to affect the production rate of certain metabolites in media supplied with fixed nitrogen. For example, production of levan was markedly reduced if the impeller agitation speed was increased from 300 to 600 rpm (Berzins et al., 2001) although the growth of the biomass was only marginally affected. Similarly, in anaerobic continuous cultures, the steady-state biomass concentration was found to progressively increase with increasing agitation speed in the bioreactor, but biomass-specific ethanol productivity at steady-state declined as the turbulence level increased in media containing fixed nitrogen (Toma et al., 2003). ...
... Levan is primarily found as a microbial exopolysaccharide and is a fructose biopolymer mainly linked by β-(2, 6)-glycosidic bonds, with β-(2, 1)-linked side chains [19,40,76,125]. Levan is also a fructan of higher plants that has mostly the (2, 6) fructosyl-fructose linkage and it consists of high degree of polymerisation (DP), (DP > 100) polymers such as those found in bacterial systems [33,61]. Phlein are plant-derived compounds which contain mostly the (2, 6) fructosyl-fructose linkage and these polymers are of lower molecular weight (DP < 100) [61]. ...
... The introduction of strong electron-accepting groups (2c and 4c) significantly decreased both the extinction coefficients and quantum yields. The absorption spectra of the recently examined benzanthrone alkyl and aryl sulfides spanned from 403 to 448 nm [44], and amidines absorbed in the range of 410 to 495 nm [45,46], whereas 3-substituted benzanthrone amino derivatives exhibited absorption between 430 and 520 nm [47]. For 3-amino-9-nitrobenzanthrone derivatives, absorption occurred in the range of 450 to 560 nm [48]. ...
... This can be performed by using installations such as baffles or external particles/support materials providing the attachment of the cells on the surface (Muffler et al., 2014;Oliveira and Melo, 2003). For this purpose, woven balls made from stainless steel wire gauzes (Atkinson et al., 1979), stainless steel wire modified by TiCI 4 and γ-aminopropyltriethoxysilane (to improve cell immobilization), silanized stainless steel (Karsakevich et al., 1998), and copper (Schwartz et al., 2003) have been used as inorganic biofilm support materials. Nevertheless, the porosity of the support materials is also one of the significant factors influencing the performance of the formed biofilm when employed for conversion processes. ...
... The levan was highly stable and withstand a wide range of pH. The influence of physicochemical factors on levan were studied and results showed that levan structure was stable at a pH 4-11 and high temperatures up to 70 • C (Vina et al., 1998). The supernatant was maintained at pH 10 and by use of three different kinds of solvents separately and each solvent was used at four different ratios were added to the supernatant and yield was measured. ...
... have shown an increase from 25% to 100% in PHB accumulation with respect to the cell dry weight. 13,14 In a stirred fermenter, the OTR can be varied by changing the agitation rate. Díaz-Barrera et al. 15 ...
... The result corresponds to other studies [29] in which Y. lipolytica cells showed 76.5-80.2% hydrophobicity when grown on a mixture of C 12 -C 16 alkanes, but after seven days of incubation. Our results suggest a higher ability of Y. lipolytica CMGB32 cells to adapt to hydrocarbons substrates, such as n-hexadecane. ...