Stine G. Jessing's research while affiliated with Technical University of Denmark and other places
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Publications (5)
The aim of the present study was to investigate the organisation of the genes (cps) involved in biosynthesis the capsular polysaccharide (CPS) of Actinobacillus pleuropneumoniae serotypes 6, 7, and 12 and to compare these to the corresponding genes previously described in other A. pleuropneumoniae serotypes. In serotypes 6 and 7 the sequenced DNA r...
A PCR assay for simultaneous species identification and separation of Actinobacillus pleuropneumoniae serovars 1, 7 and 12 was developed. Primers specific for genes involved in biosynthesis of the capsular polysaccharides (cps genes) of serovars 1, 7, and 12 were combined with a species-specific PCR test based on the omlA gene. The PCR test was eva...
Multiplex PCR assays were developed to identify Actinobacillus pleuropneumoniae serotypes 1, 2, and 8. Primers designed for the conserved capsular polysaccharide (CP) export region amplified a 489-bp DNA
fragment from all serotypes. Primers specific to the CP biosynthesis regions of serotypes 1, 2, and 8 amplified fragments
of 1.6 kb, 1.7 kb, and 9...
Serotype-specific DNA regions involved in the biosynthesis of capsular polysaccharides (cps region) were used to develop a multiplex PCR test for the simultaneous species identification and serotyping of Actinobacillus pleuropneumoniae serotypes 2, 5, and 6. Primers specific for serotypes 2, 5, and 6 were combined with the already existing species-...
Citations
... Increasingly, laboratories are using molecular typing methods to more accurately and reproducibly identify A. pleuropneumoniae isolates (Gottschalk, 2015;Sassu et al., 2017). PCRs have been developed for detection of specific CPS genes in most of the currently recognized 18 serovars (except 4, 9, 11, 13 and 14), either individually or in multiplex reactions for detection of predominant serovars in a given geographical region ( Angen et al., 2008;Bossé et al., 2014;2018;Ito and Sueyoshi, 2015;Jessing et al., 2003;Schuchert et al., 2004;Turni et al., 2014). Some of these PCR assays were developed prior to the availability of whole genome sequences (wgs), and were based on (sometimes incomplete) sequences of the CPS biosynthetic loci. ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/273662737645579-1442257689704_Q64/Oystein-Angen.jpg)
... The detection of Apx I-III-toxin is of particular interest, since the expression of Apx-toxin is the crucial factor to estimate the virulence of the different A. pleuropneumoniae serotypes (ST) [13]. The differentiation of serotypes can be achieved using a multiplex PCR system (mPCR) that targets the gene sequences of Apx I-III [14], Apx I-III in combination with omlA protein [15], and capsular polysaccharides (cps) [16] alone or in combination with omlA protein [17]. Furthermore, the continuous advancement of A. pleuropneumoniae mPCRs within the last years enables the differentiation of the A. pleuropneumoniae ST 1-19 [18,19]. ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/272453024874535-1441969271578_Q64/Thomas-Inzana.jpg)
... As specificity was shown to be related to differences in capsular structures (Perry et al., 1990), PCRs targeting capsule synthesis genes have been developed as a more reliable method to differentiate serovars, overcoming the problems of lack of standardization between laboratories, inter-batch serum variation and crossreactivity between serovars seen with serological typing. Early capsule typing PCRs (Jessing, Angen, & Inzana, 2003;Lo, Ward, & Inzana, 1998;Schuchert, Inzana, Angen, & Jessing, 2004;Zhou et al., 2008) were limited by the extent of genetic data available at the time and often exploited detection of the conserved export genes, known to be divergently transcribed from the specific biosynthesis genes in members of the Pasteurellaceae and other Gram-negative bacteria (Frosch, Edwards, Bousset, Krauße, & Weisgerber, 1991), for identification of partial or complete capsule biosynthetic loci. Development of mPCRs initially focused on limited numbers of serovars (often those relevant to the geographic location), with tests for discrimination of serovars 2/5/6 (Jessing et al., 2003), 3/6/8 , 1/7/12 (Angen, Ahrens, & Jessing, 2008), 5/7/12/15 (Turni, Singh, Schembri, & Blackall, 2014), and 1/2/5/7/15 (Ito & Sueyoshi, 2015) described. ...
... Діагноз на актинобацилярну плевропневмонію свиней установлюють на основі клінічних симптомів, патологоанатомічнихмакроскопічних та гістопатологічних, бактеріологічних досліджень уражених легенів або змивів із гортані, визначенні серологічного профілю (ІФА), за дослідження ізолятів мікроорганізмів або патматеріалів методом ПЛР [11,12]. Але лабораторна діагностика при цьому досить складна, що пов'язано із вимогливістю цих мікроорганізмів до живильних середовищ та їх швидкою загибеллю поза організмом тварини. ...
... Early capsule typing PCRs (Jessing, Angen, & Inzana, 2003;Lo, Ward, & Inzana, 1998;Schuchert, Inzana, Angen, & Jessing, 2004;Zhou et al., 2008) were limited by the extent of genetic data available at the time and often exploited detection of the conserved export genes, known to be divergently transcribed from the specific biosynthesis genes in members of the Pasteurellaceae and other Gram-negative bacteria (Frosch, Edwards, Bousset, Krauße, & Weisgerber, 1991), for identification of partial or complete capsule biosynthetic loci. Development of mPCRs initially focused on limited numbers of serovars (often those relevant to the geographic location), with tests for discrimination of serovars 2/5/6 (Jessing et al., 2003), 3/6/8 , 1/7/12 (Angen, Ahrens, & Jessing, 2008), 5/7/12/15 (Turni, Singh, Schembri, & Blackall, 2014), and 1/2/5/7/15 (Ito & Sueyoshi, 2015) described. With availability of increased genetic data, including whole genome sequences for many serovars (Foote et al., 2008;Xu et al., 2008Xu et al., , 2010Zhan, Angen, Hedegaard, Bendixen, & Panitz, 2010), an extensive mPCR was developed to discriminate serovars 1-3, 5-8, 10 and 12 (Boss e et al., 2014), which has subsequently been expanded to cover all known serovars of A. pleuropneumoniae, including the recently identified serovars 16-18 (Boss e et al., 2018;Sárk€ ozi et al., 2015). ...
