S Zaremba's research while affiliated with National Institutes of Health and other places

... Studies have identified PSA-derived peptides that activate tumor-reactive CD8+ cytotoxic T lymphocytes (CTLs) when presented by human leukocyte antigen-A2 (HLA-A2) and HLA-A3 [75][76][77] . Simultaneous induction of tumor-reactive CTLs and HLA-A2/A3-restricted epitopes of PSA has been achieved using specific oligopeptides [78][79][80] . Additionally, HLA-A24-restricted PSA peptides elicited peptide-specific CTLs in PCa patients and HLA-A*2402restricted CTLs in transgenic mice [81][82][83] . ...

... In many brain regions, PNNs preferentially enwrap inhibitory parvalbumin-positive neurons, which are highly active and involved in critical periods of brain development, and play a crucial role in the regulation of neuronal functions and synaptic plasticity (Cabungcal et al., 2013;Fawcett et al., 2019;Reichelt et al., 2019). In the cerebellar cortex, Lugaro/globular cells, which receive strong GABAergic inhibition from PCs via their axon collaterals, are recognized by a monoclonal antibody against aggrecan, Cat-301 (Sahin and Hockfield, 1990;Zaremba et al., 1990;Crook et al., 2007), which means that they are surrounded by PNNs. On the other hand, both the cell bodies and the proximal dendrites of large and excitatory DCN neurons, which are primarily glutamatergic (Telgkamp and Raman, 2002;Uusisaari et al., 2007), are surrounded by PNNs (Figures 1C, 3A) in which aggrecan is the primary CSPG (Zaremba et al., 1990;Carulli et al., 2004Carulli et al., , 2006Carulli et al., , 2007Zimmermann and Dours-Zimmermann, 2008;Foscarin et al., 2011;Bekku et al., 2012). ...

... We have investigated the ability of an oligopeptide, PSA-OP, to induce cytotoxic T cell immune responses against PSA. PSA-OP is a 30-mer peptide containing three HLA-A2-binding epitopes (27), as well as an epitope for HLA-A3 (44) and, potentially, HLA-A11 (that shares a common anchor motif of V at position 2 and K at position 9) (45). In these studies, we have generated two HLA-A2-restricted cytotoxic T cell lines (T1-PSA-OP and T2-PSA-OP) and two HLA-A3-restricted cytotoxic T cell lines (T3-PSA-OP and T4-PSA-OP) from two HLA-A2 donors and two HLA-A3 donors, respectively. ...

... In an attempt to overcome the shortcomings of murine and intact IgG and to profit from the excellent specific reactivity of CC49, Slavin-Chiorini bioengineered a CDRgrafted humanized monoclonal antibody with a C H 2 domain deletion (ΔCH2) [98]. Deletion of the C H 2 domain of IgG had already been reported to result in faster tumour uptake and more rapid blood clearance [99][100][101]. The recombinant IgG molecule HuCC49ΔCH2 combined, for the first time, a fast blood clearance with the reduced potential for eliciting a HAMA response. As anticipated, radioiodinated ( 125/131 I) HuCC49ΔCH2 constructs demonstrated faster blood clearance in both athymic and SCID mice bearing human colon carcinoma xenografts and effective localization to tumour xenographs while showing minimal deposition in healthy tissues [98,101,102]. ...

... Developmental formation of PNNs is activity-dependent, and several brain regions including barrel cortex, thalamus, visual cortex, and vocal center in songbirds show underdeveloped PNNs if deprived of activity, suggesting a high malleability of PNNs (Guimarães et al., 1990;Lander et al., 1997;Pizzorusso et al., 2002;McRae et al., 2007;Nakamura et al., 2009). Although PNNs in the mature CNS appear to be largely stable in a normal physiological state, emerging evidence suggests bidirectional changes in the structure and numerical density of PNNs on a cyclic basis as well as under specific conditions such as drug addiction, maternal hormone fluctuations, and chronic pain (Lasek et al., 2018;Pantazopoulos et al., 2020;Uriarte et al., 2020;Harkness et al., 2021;Mascio et al., 2022). ...

... The ECM also has an important role in regulating synaptic function and development [37]. As the brain develops postnatally, the composition of the ECM changes to facilitate this function [38,39]. In the early stages of development, the ECM is dynamic and permis-Int. ...

... In macaques, stripes of the same type have a center-to-center distance of around 4.0 mm and a width ranging from 0.7 to 1.3 mm (Shipp and Zeki, 1985;Tootell and Hamilton, 1989). In humans, these widths are approximately doubled in size (Hockfield et al., 1990;Tootell and Taylor, 1995;Adams et al., 2007). ...

... Neural cell-adhesion molecule (N-CAM, Zaremba et al. 1990 ) and neuron-specific class III βtubulin isotype (Dráberová et al. 1998), both being the markers of neural cells, and endoderm-specific cytokeratin Endo-A (Kanungo et al. 2000) were quantified by Western blot analysis. For Western blot analysis, both cultured EC cells and mouse fetal brain tissue (developing neopallium) were processed according to same protocol. ...

... Theyspeculated that the structure might be involved in insulating neurons (28). Later studies described PNN composition and the development of new antibodies for Chondroitin sulfate proteoglycans (CSPGs) (29,30), which were used to investigate individual CSPGs, particularly in the context of glial scar formation (31). The use of ChABC to disrupt PNN structure by degrading GAGs and thus removing the PNN that has been deposited around neurons, resulted in memory loss and reopening of critical periods. ...

... IC a number of other proteoglycans have been shown to support synaptic function. Aggrecan has been shown to stabilize synaptic structure, while brevican and neurocan are important for the maintenance of inhibitory and excitatory synapses (Zaremba et al., 1989;Gottschling et al., 2019;Schmidt et al., 2020). The proteoglycan neurocan is also known for its roles in the development of PNs, while phosphacan is better understood in the aging brain (Nishiwaki et al., 1998;Gottschling et al., 2019;Schmidt et al., 2020). ...