R. S. Simon's scientific contributions
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It was automatically created by ResearchGate to create a record of this author's body of work. We create such pages to advance our goal of creating and maintaining the most comprehensive scientific repository possible. In doing so, we process publicly available (personal) data relating to the author as a member of the scientific community.
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Publication (1)
We have developed a new vector strategy for the insertion of foreign genes into the genomes of gram negative bacteria not closely related to Escherichia coli. The system consists of two components: special E. coli donor strains and derivatives of E. coli vector plasmids. The donor strains (called mobilizing strains) carry the transfer genes of the...
Citations
... First, we created the plasmid pMVRha (8437 bp) ( Figure 3C), a Golden Gate-compatible plasmid based on the pCAT201 backbone (Table 1). It contains the par stabilizing region 35 , the origin of transfer from RP4 (oriT) 11 , a rhamnose-inducible promoter (RhaRS + P Rha ) 50 , BsaI restriction sites, and a chromoprotein gene as cloning marker (spisPink) 51 . Colonies containing un-restricted pMVRha show pink color on plate. ...