M. Lupak’s research while affiliated with Ivan Franko National University of Lviv and other places

What is this page?


This page lists works of an author who doesn't have a ResearchGate profile or hasn't added the works to their profile yet. It is automatically generated from public (personal) data to further our legitimate goal of comprehensive and accurate scientific recordkeeping. If you are this author and want this page removed, please let us know.

Publications (3)


Leukocyte actin cytoskeleton reorganization and redistribution of sialylated membrane glycoconjugates under experimental diabetes mellitus and against the administration of the Galega officinalis L. extract
  • Article

May 2017

·

27 Reads

·

2 Citations

Cytology and Genetics

M. Lupak

·

H. Hachkova

·

·

[...]

·

The article describes the effect of alkaloid-free fraction of the Galega officinalis extract (AFFGE) on the aggregation ability of immunocompetent blood cells, as well as on the process of actin polymerization and structural rearrangements among sialylated glycoconjugates of the peripheral blood leukocyte membranes of rats in the norm and under experimental diabetes mellitus (EDM) conditions. The flow cytometry method (using phalloidin labelled with fluorescent tetramethyl rhodamine-5-isothiocyanate (TRITC)) and the western blot analysis have allowed us to detect an increase in the rat leukocyte F-actin content in the event of diabetes mellitus, which indicated changes in the structural and functional properties of the leukocytes and their preactivation phase. A quantitative analysis of the total polymerized actin pool redistribution between its constituent fraction (represented by cytoskeletal filaments) and short actin filaments has shown that, against an increase in the total F-actin level, the number of actin filaments of the cytoskeleton decreased and the content of short actin filaments increased in leukocytes of animals with EDM. The use of sialylated lectins has allowed a conclusion to be made on the study of the pathology that the number of exposed oligosaccharide determinants on leukocyte membrane, the structure of which contained N-acetyl-β-D-glucosamine and sialic acid residues, increased, whereas the number of sialic acid-containing surface glycoconjugates bound to subterminal galactose residues by α2→3 and α2→6-glycoside bonds decreased. The administration of AFFGE to diabetic animals led to an increase in the content of F-actin and short filaments of the leukocyte cytoskeleton and a reduction in the lectin-induced leukocyte aggregation. The correction effect of the studied extract on the functional state of leukocytes can be realized through the action on the processes underlying the formation of the actin cytoskeletal elements and due to the quantitative redistribution of leukocyte membrane glycoconjugates with different structures of carbohydrate determinants, such as, due to a decrease in the exposure of N-acetyl-β-D-glucosamine residues and an increase in the exposure of sialic acids bound to subterminal galactose residues by α2→3 and α2→6-glycoside bonds.


Fig. 1. Water emulsion and its stabilized forms of alkaloid-free fractions isolated from Galega officinalis extract with PS biocomplex. Сoncent ration of PS biocomplex (g/l): A-0 (control); Б-0.6 g/l; В-2.0 g/l; Г-3.3 g/l 
Fig. 2. Microphotographs of alkaloid-free fraction emulsion from Galega officinalis extract and it's stabilized forms with PS biocomplex. Сoncentration of PS biocomplex (g/l): A-0 (control); Б-0.6 g/l; В2.0 g/l; Г-3.3 g/l 
Fig. 3. Effect of alkaloid-free fraction from Galega officinalis extract and it's stabilized form with PS biocomplex on glucose and glycosylated hemoglobin under EDM (М±m, n = 5-10). *-P<0.05 as compared with control group #-p <0.05 as compared with EDM group 
Application of biogenic surfactants for stabilization on alkaloid-free fraction isolated from Galega officinalis extract
  • Article
  • Full-text available

March 2015

·

107 Reads

·

5 Citations

Studia Biologica

The article contains data on the influence of biogenic surfactants synthesized by bacteria Pseudomonas sp. PS-17 on the stability of emulsions based on alkaloid-free fraction isolated from Galega officinalis L. extract. The opti­mum concentrations of biogenic surfactants to stabilize investigated emulsionswere experimentally chosen. The chemical composition of alkaloid-free fractions from Galega officinalis L. extract before and after stabilization were investigated. The resulting substance is easier to administer to animals through a tube, which not only improves the accuracy of the dosage, but also increases its bioavailability. Reduction of glycosylated hemoglobin at 13.4 % and glucose content to the physiological values in the rats blood with experimental diabetes mellitus by administration within 14 days stabilized emulsions compared with the original emulsion shows that emulsion stabilized by the biogenic surfactant based on alkaloid-free fraction isolated from Galega officinalis an extract provides effective hyperglycemia compensation in rats under experimental diabetes mellitus, compared with the original mixture, which may be caused by changes in the proportion of biologically active substances in the extract while stabilizing and its better bioavailability.

Download

Fig. 1. Typical curves SNA-(A) and MAA-(B)-induced aggregation of leukocytes under conditions of GOE nonalkaloid-containing fraction administration to healthy animals and to animals with EDM  
The inhibition of rat leukocytes apoptosis under the condition of experimental diabetes mellitus type 1 by Galega officinalis L.extract

December 2013

·

126 Reads

·

5 Citations

Current Issues in Pharmacy and Medical Sciences

The article contains the data of Galega officinalis extract (GOE) nonalkaloid-containing fraction influence on the structural and functional state of leukocytes under the condition of experimental diabetes mellitus type 1 (EDM). Immunohistochemical method has shown the quantitative redistribution of leukocytes containing proapoptotic protein p53 and PARylated [poly(ADP-ribosyl) ated] proteins under EDM conditions and admission of the tested extract. GOE administration to animals with EDM leads to the reduction in the number of cells with positive and strongly positive reaction to the p53 protein and PARylated proteins content, indicating its depressing effect on apoptosis of immune cells. 2013

Citations (3)


... Emulsions as dispersed systems with a developed surface of phase separation, which has an excess of free surface energy, are characterized by thermodynamic instability in the form of coalescence, when individual phases of the emulsion are separated. The stability of emulsions depends on such factors as the nature of the dispersion medium and the dispersed phase, the amount of surface tension, and the viscosity of the system [9,10]. ...

Reference:

Modelling of the composition of emulsion medicines and cosmetics stabilized by a biocomplex of surfactant substances based on rhamnolipids Pseudomonas sp. PS-17
Application of biogenic surfactants for stabilization on alkaloid-free fraction isolated from Galega officinalis extract

Studia Biologica

... In animals with diabetes, the non-alkaloid fraction of Galega officinalis extract causes a decrease in filamentous actin (F-actin) content; this can testify about the reduction in the formation of short pseudopodia on the leukocytes surface. These data indicate that the use of this extract reduces the change in the structural and functional properties of leukocytes, as well as decrease of leukocyte pre-activated state [67]. It is possible that the extract-induced decrease in actin polymerization might regulate integrin-dependent interaction with vascular endothelium necessary for leukocytes penetration through the blood vessel wall during inflammatory processes [68]. ...

Leukocyte actin cytoskeleton reorganization and redistribution of sialylated membrane glycoconjugates under experimental diabetes mellitus and against the administration of the Galega officinalis L. extract
  • Citing Article
  • May 2017

Cytology and Genetics

... Under streptozotocin-induced diabetes, the administration of the non-alkaloid fraction of Galega officinalis extract increases the content of α(2→3)-bond sialic acids to physiological levels. It is possible that this effect is due to the influence of the extract's biologically active substances on the activity of enzymes involved in the cleavage or transfer of sialic acid residues (neuraminidase and trans-sialidase) [67,76]. Glycoproteins that contain sialic acids are structural components of the leukocyte co-receptor complex CD3, which is present in all mature T-lymphocytes and is involved in their activation. ...

The inhibition of rat leukocytes apoptosis under the condition of experimental diabetes mellitus type 1 by Galega officinalis L.extract

Current Issues in Pharmacy and Medical Sciences