John M. Pemberton's research while affiliated with The University of Queensland and other places
What is this page?
This page lists the scientific contributions of an author, who either does not have a ResearchGate profile, or has not yet added these contributions to their profile.
It was automatically created by ResearchGate to create a record of this author's body of work. We create such pages to advance our goal of creating and maintaining the most comprehensive scientific repository possible. In doing so, we process publicly available (personal) data relating to the author as a member of the scientific community.
If you're a ResearchGate member, you can follow this page to keep up with this author's work.
If you are this author, and you don't want us to display this page anymore, please let us know.
It was automatically created by ResearchGate to create a record of this author's body of work. We create such pages to advance our goal of creating and maintaining the most comprehensive scientific repository possible. In doing so, we process publicly available (personal) data relating to the author as a member of the scientific community.
If you're a ResearchGate member, you can follow this page to keep up with this author's work.
If you are this author, and you don't want us to display this page anymore, please let us know.
Publications (8)
Albicidin blocked DNA synthesis in intact cells of a PolA- EndA- Escherichia coli strain, and in permeabilized cells supplied with all necessary precursor nucleotides, indicating a direct effect on prokaryote DNA replication. Replication of phages T4 and T7 was also blocked by albicidin in albicidin-sensitive (Albs) but not in albicidin-resistant (...
A DNA fragment containing a gene for resistance to the antibiotic albicidin was isolated from Klebsiella oxytoca and shown to be expressed in Escherichia coli , where it also protected bacteriophage T7 replication from inhibition by albicidin. In vivo translation analysis demonstrated that the cloned 2.2kb DNA fragment coded for a 36 kiloDalton (kD...
The structural gene for excreted amylase from Aeromonas hydrophila JMP636 has been cloned within a 2.1-kilobase SmaI fragment of DNA. The amylase gene is transcribed from its own promoter in Escherichia coli, producing a gene product of Mr 49,000. The amylase gene product is secreted to the periplasm of E. coli; however, it is not excreted. Nucleot...
DNA fragments containing theKlebsiella oxytoca genes encoding -glucosidase and amylase were cloned into the kanamycin resistance transposon Tn5. Another DNA fragment containing two genes for polygalacturonatetrans-eliminase was cloned into Tn1721. These newly constructed transposons were then each transposed in vivo onto the broad-host-range plasmi...
The carotenoid photopigment genes of the purple nonsulfur photosynthetic bacteriumRhodospeudomonas sphaeroides have been cloned into the kanamycin resistance transposon Tn5 to create a carotenoid transposon-Tn5-Crt+. Transposition of Tn5-Crt+ onto the broad host range plasmid pR751 produced a broad host range carotenoid plasmid-pJP115. Transfer of...
A DNA fragment containing a Klebsiella oxytoca gene for polygalacturonate trans-eliminase was cloned into the kanamycin resistance transposon Tn5. This new transposon, designated Tn5-Pga
+, had a transposition frequency of 1×10-6. The broad host range plasmid pR751::Tn5-Pga
+ was conjugally transferred to a variety of genetic backgrounds. The abili...
Plasmid pULB113 (RP4::Mini-Mu) promoted homologous gene transfer inAeromonas hydrophila; transfer of chromosomal markers occurred at frequencies of between 10–3 and 10–4 per donor cell regardless of the marker selected; this indicated chromosome transfer from multiple origins. With a variety of amino acid biosynthetic markers, a single circular map...
Two cosmids, pJP1433 and pJP1488, have been constructed which carry between them 60 kilobases of the central section of the photosynthesis region ofRhodopseudomonas sphaeroides. Within this region is a segment of some 15 kilobases which encompasses the known carotenoid biosynthesis genesCrtA,CrtB,CrtC,CrtD,CrtE, andCrtF.
Citations
... (i) Does PpaA affect PpsR-mediated repression of puc expression in a heterologous host? Paracoccus denitrificans, a non-photosynthetic relative of R. sphaeroides, is capable of expressing PS genes when these are introduced in trans (Pemberton & Harding, 1987). P. denitrificans lacks specific regulators of PS gene expression, which makes it a convenient host to examine PS gene regulation (Gomelsky & Kaplan, 1995a, 1997). ...
... GAS cultures were routinely grown at 37°C, with liquid GAS cultures grown without agitation. Escherichia coli strains were grown on Z agar (Walker and Pemberton, 1988) or in Luria Bertani (LB) broth (Sambrook et al., 1989). Where appropriate, strains were grown with 100 μg/ml of ampicillin and 50 μg/ml of kanamycin, for plasmid retention. ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/272403121045539-1441957373659_Q64/Mark-Walker-17.jpg)
... Genetic manipulation of bacteria can be hampered by the presence of extracellular and periplasmic DNases. Previous studies involving Aeromonas hydrophila JMP636 have not been successful in transformation of this strain, with genetic transfer accomplished by conjugation [1,2]. Poor transformation of organisms by foreign DNA has previously been observed in other Gram-negative bacteria such as the related Vibrio cholerae. ...
... Rhizobium and Paracoccus (Pemberton and Harding, 1986, 1987). Subsequent expression of the Erwinia carotenoid gene cluster in Escherichia coli lead to the production of novel carotenoids by combinatorial synthesis (Lee et al., 2003). ...
... Under ongoing selective pressure, GDAs can provide a basis for the evolution of genes with new functions, as the additional gene copies could potentially evolve independently, acquiring new functions or specificities [40,47], e.g., to diversify or specialize an existing resistance mechanism or generation of new fusion proteins. In this study, it is conceivable that overexpression of STM3175 by GDA events paves the way for fixed, genetic albicidin resistance by subsequent mutations in the Tsx nucleoside channel, as shown for E. coli [22]. ...
... strain KFCC 10818 [37,38], Vibrio sp. strain M12-1144 [77], V. cholera strain 4670, Aeromonas hydrophila strain JMP636 [78], and V. splendidus strain 0407ZC148 [79]. ...
... There are two types of mechanisms: reversible detoxification and irreversible detoxification. In reversible detoxification, the protein synthesized is bound reversibly to the toxin in bacteria like Klebsiella oxytoca (Walker et al., 1988) and Alcaligenes denitrificans (Basnayake and Birch, 1995). Later mechanism was observed in Pantoea dispersa where irreversible detoxification of albicidin was mediated by esterase (Zhang and Birch, 1997). ...
... Plasmids from E. coli strains O6877 and D275 were conjugated with E. coli DH5α as previously described [32]. Gel electrophoresis of plasmid preparations showed that the wildtype strains carried several plasmids of different molecular size. ...
