Joann M Kinyon's research while affiliated with Iowa State University and other places

... Multiple studies that have evaluated commercially available or autogenous vaccines against IBK have reported lack of efficacy of these parenterally administered vaccines [24][25][26][27]. An experimental parenteral vaccine that used a partially purified native M. bovis cytotoxin as antigen showed some efficacy against IBK [28]. ...

... Até ao momento não há profilaxia vacinal disponível para gatos, apesar da sua larga utilização em espécies pecuárias. O interesse particular das vacinas seria na proteção contra as doenças do TRS, em que a quantidade de imunoglobulina A (IgA) é crítica na imunização contra o agente, e a vacinação intranasal é experimentalmente efetiva ao conferir imunidade não específica (Bradley et al., 2012;Greene and Chalker, 2012). ...

... 222 Liu et al. 223 have used the pig to study the effects of non-penetrating ballistic impact on the brain, and the pig has been employed as an experimental model of intracranial hypertension. 224 The pig has also been used to model the following conditions: type 1 diabetes, 201 diabetic wound healing, 225 non-healing in bone, 226 human autosomal dominant retinitis pigmentosa, 227 glaucoma, 228 fulminant hepatic failure, 229 ischaemic heart disease, 230 congestive heart failure, 231 bariatric surgery on obesity, 232 bacterial infection, [233][234][235][236] Trypanosoma cruzi infection, 237 viral myocarditis, 238 cystic fibrosis 239 and Duchenne muscular dystrophy. 240 The pig has also been recommended as a surgeon's training model for human surgery (e.g. for axillary lymphadenectomy). ...

... Carvajal et al. (2006) found 32.1% of 421 pig farms positive for the agent, but samples were taken from pigs (5-20 per farm) with clinical signs such as diarrhoea or from pigs with decreased growth rate. A US study by Duff et al. (2014) estimated in one endemically SD-infected farm the prevalence of carriage in sows and suckling pigs at different time points. The proportion of sows positive for B. hyodysenteriae ranged from 0% to 5% with an overall prevalence of 1.04%. ...

... When human saliva samples-collected with a specific device-were stored for one month in ambient temperatures in a tropical country, the antibodies against HIV were successfully preserved [30]. Similarly, anti-PRRSV antibodies in pig oral fluid were relatively resistant to degradation over 12 days, and the authors suggested that appropriate specimen-handling protocols, including prompt freezing or refrigeration at 4 • C, would maintain the integrity of anti-PRRSV antibodies in oral fluid samples [63]. ...

... C. pseudotuberculosis biovar equi is the etiological agent of ulcerative lymphangitis (UL), commonly called "pigeon fever" (5). UL has several symptoms, however, the most common clinical manifestation of this disease is characterized by external pectoral or ventral abscesses (6). ...

... Throughout the period under review there were only a few publications dealing with S. suis coinfections (n = 9/68). These publications included infection models with piglets experimentally infected with porcine respiratory and reproductive syndrome virus (PRRSV) [33][34][35][36][37] (n = 5/9), Bordetella bronchiseptica [38,39] (n = 2/9), porcine circovirus type 2 [40] or swine influenza virus [41] (each n = 1/9), followed by S. suis infection. Coinfection was used as a predisposing factor to promote the clinical manifestation of S. suis infection. ...

... Definitive disease diagnosis of SD is typically based on selective anaerobic culture with isolation of a strongly bhemolytic Brachyspira spp. that produces a positive ring phenomenon from the colonic mucosa or feces of clinically affected pigs, by polymerase chain reaction (PCR) assays, or a combination of both assays (Hampson and Burrough, 2019). Fluorescent ISH assays have also been developed and can aid the identification of B. hyodysenteriae and B. hampsonii in formalinfixed tissues (Burrough et al., 2013;Wilberts et al., 2015;Hampson and Burrough, 2019). However, a diagnostic tool that would help predict or precede disease development, or aid in monitoring disease progression is lacking. ...

... On the other hand, several USA studies indicate high concentration of insoluble fiber in the diet promotes expression of SD. For example, the addition of 30% distillers' dried grains with solubles (DDGS) shortens the incubation period of clinical signs and shedding after challenge with strongly hemolytic B. hampsonii (strain EB107) or B. hyodysenteriae (strain B204) [43]. DDGS are co-products from the biofuel and milling industries used in swine diets to reduce the cost of feeding. ...

... Six isolates were also identified using biochemical identification according to the strength of haemolysis and the determination of α-galactosidase, α-glucosidase, β-glucosidase, and hippurate activity using established procedures [23]. Species identification by MALDI-ToF mass spectrometry was performed as described previously [24]. A custom library encompassing the main spectra of the commercial Bruker library, the main spectra validated by Warneke et al. [24] [25] were preliminarily assessed on TSA with 10% bovine blood at 42 °C with isolates not showing a delineated zone of translucency around an agar punch hole being designated as weakly haemolytic. ...