Jingsan Sun's research while affiliated with Technical Institute of Physics and Chemistry and other places
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Publications (10)
srs-1, a new floral organ identity gene in rice, was mapped using RAPD and RFLP markers. Firstly, the cross was made between “ZhaiYeQing
8” (ZYQ8, indica) and split rice spikelet (SRS, japonica) mutant. The ratio of wild-type individuals and mutant plants in
F2 population is 3:1, which indicates that the mutant characteristics are controlled by sin...
Plastid is one of the most important cellular organelles, the normal division process of plastid is essential for the differentiation
and development of plant cells. For a long time, morphological observations and genetic analyses to special mutants are the
major research fields of plastid division, but the molecular mechanisms underlying plastid d...
FtsZ protein plays an important role in the division of chloroplasts. With the finding and functional analysis of higher plant FtsZ proteins, people have deepened the understanding in the molecular mechanism of chloroplast division. Multiple ftsZ genes are diversified into two families in higher plants, ftsZl and ftsZ2. On the basis of the research...
In order to elucidate the origin of the plastid division gene ftsZ in green plant lineage, and to understand the significance of this divergence for the function of FtsZ proteins in plants,
two full‐length cDNAs (accession numbers AF449446 and AB084236) were isolated from Chlamydomonas reinhardtii, a base species of green plant lineage. A phylogene...
Polygalacturonase-inhibiting proteins PGIP play important roles in plant defense of pathogen especially fungi. A pair of degenerated primers is designed based on the conserved sequence of 20 other known pgip genes and used to amplify Gossypium bar-badense cultivation 7124 cDNA library by touch-down PCR. A 561 bp internal fragment of the pgip gene i...
The glucose oxidase (GO) gene with the function of broad-spectrum pathogen defense was inserted into a binary vector pCAMBIA1301 containing selectable marker of hygromycin resistant gene to produce a new expression vector, pCAG1301. The vector was introduced into Agrobacterium tumefaciens strain LBA4404 and then transformed them to immature embryos...
Two clones of a new family of tandemly repeated DNA sequences have been isolated from a maize random genomic DNA library. MR68 is 410 bp, representing a monomeric unit and MR77 is 1222 bp, containing three units. The copy number was estimated to be about 3000 per 1C maize genome. Its methylation pattern was also determined. Fluorescent in situ hybr...
A simple and rapid regeneration method of cotton (Gossypium hirsutum L. cv. Xinluzao 4) is described. The proper use of phytohormone KT and IBA validly promoted the survival rate of test-tube
plants and shortened the period of culture in combination with the techniques of micro-propagation and graft.
The green fluorescent protein (GFP) gene from the jellyfishAequorea victoria as a vital reporter for gene expression in plants is considered to have several advantages over other reporter genes. The
pBIN35S-mGFP4 plasmid DNA has been introduced into cotton embryos by the pollen-tube pathway method. A transformed seedling
has been verified according...
Nearly 100 maize-specific repeated DNA clones were screened from a maize random genomic library, and used for RFLP analysis
of two wheat DH populations from wheat and maize crosses. The result showed that a maize fragment in clone MR64 was transferred
into two wheat DH lines, i.e. No. 18 in common wheat DHs and No. 15 in persicum wheat DHs, in whic...
Citations
... Nicotiana tabacum is an important model organism in plant science. Its FtsZ genes belong to two distinct families, FtsZ1 and FtsZ2 (El-Shami et al. 2002;Wang et al. 2002a, c;Kong et al. 2003). Previous study has shown that abnormal expression of NtFtsZ1 family genes from N. tabacum could lead to abnormal chloroplast division (Wang et al. 2002a, c). ...
... The MADS family is an ancient and broadly studied transcription factor and plays essential roles in almost all developmental processes in plants, such as floral organ development [49,50], controlling the flowering time of plants [51], ovary development, seed-coat development [52,53], embryo development [54], determining the meristem [55], root growth [56], plant vegetative growth [57] and symbiosis induction [58], fruit development and ripening [59], silique architecture [60], modulating plant architecture and abscisic acid [61], and orchid reproductive development [62]. However, less attention is paid to stresses regulated by MADS-box genes in plants. ...
... In contrast, the pollen tube transformation (PTT) method involves transferring a foreign gene into peanut embryos after self-pollination ( Zhou et al. 1983). Since it was developed, this technique has been widely applied to cotton (Zhou et al. 1983;Wang et al. 2013;Huang et al. 1999), melon (Hao et al. 2011), soybean (Hu andWang 1999;Li et al. 2002;Shou et al. 2002;Yang et al. 2011), wheat (Martin et al. 1992), and maize (Zhang et al. 2005;Yang et al. 2009). ...
... The embryogenic callus (EC) and somatic embryo (SE) induction were related to the 2,4-D concentration, which decreased to a certain level or was substituted by the combination of IBA and KT in the medium (Sun et al., 2006;Kumar et al., 2015). Zhu and Sun (2000) found that callus cultured with IAA, 2,4-D, and KT simultaneously had a better effect. At the same time, callus induced by 2,4-D had to be transferred to the medium with 2,4-D replaced or removed to yield SE. In this experiment, the concentration of 2,4-D decreased from 0.1 to 0.05 mg L −1 , which was beneficial to the callus differentiation. ...
... Using six glutenin loci of the Wheat × Maize decedents Kammholz et al. [98] proved their stable inheritability across generations. Furthermore Chen et al. [99] and Brazauskas [100] demonstrated the predominant genetic stability from the wheat side, and there was little or no maize DNA in the Wheat × Maize decedents. ...
... The most commonly used probes for FISH-based karyotyping in plants are the 5S rRNA and 35S rRNA genes and various noncoding repeats. Combining these landmarks with other chromosomal characters, such as total length and arm ratio, has enabled precise karyotyping in Zea mays (Chen et al. 2000) and lily (Lim et al. 2001). Recently, bacterial artificial chromosome (BAC) genomic libraries have proven to be a good source of potential chromosome-specific landmarks, especially for species with a small genome size and low repeat content. ...
... Unlike land plants that have many individual chloroplasts per cell, Chlamydomonas and most other unicellular Chlorophyte algae have a single large chloroplast that must be physically partitioned during cell division, a process that occurs before cytokinesis (Goodenough, 1970;Gaffal et al., 1995) (Figure 4). Several genes predicted to encode chloroplast division proteins in Chlamydomonas were previously shown to be expressed during S/M (Wang et al., 2003;Adams et al., 2008;Hu et al., 2008;Miyagishima et al., 2012) but were not sampled at high temporal resolution. We found that known chloroplast division genes peak at ZT11-ZT12, consistent with chloroplast division preceding mitosis and cytokinesis, whose cognate genes are expressed at ZT13 (Figures 3J and 4; Supplemental Figure 10). ...
