J Zingsem's research while affiliated with Friedrich-Alexander-University of Erlangen-Nürnberg and other places
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Publications (197)
Background:
Exposure to allogenic Human Platelet Antigens (HPAs) can lead to antibody formation causing different immunological reactions. Frequencies of common HPA antigens differ between ethnic groups and should be known to calculate potential alloimmunization risk. Syrian refugees are the largest group of applicants for asylum in Germany in 201...
Background:
A major complication after allogeneic hematopoietic stem cell transplantation (aSCT) is the reactivation of herpesviruses such as cytomegalovirus (CMV) and Epstein-Barr virus (EBV). Both viruses cause significant mortality and compromise quality of life after aSCT. Preventive transfer of virus-specific T cells can suppress reactivation...
Background:
Human neutrophil antigens (HNA) are able to provoke allo- and autoimmune antibodies which lead to reactions like autoimmune and neonatal neutropenia. However, until now no data about HNA genotype distribution in Syrian population exists. The aim of this study was to determine the HNA allele frequencies in the largest group asking for a...
HLA-A*01:234 was identified by next generation sequencing and confirmed by Sanger sequencing.
HLA-C*01:136 identified by next generation sequencing and confirmed by Sanger sequencing
Allogeneic stem cell transplantation (ASCT) to date is the only permanent curative treatment for many hematological cancers. Besides the development of Graft versus Host disease (GvHD), infections are the major adverse effect of ASCT. Specifically, reactivation of viruses is highly problematic in the aftermath of ASCT. Reactivation of human CMV and...
Background: Reactivation of CMV and EBV negatively impacts on outcome after allogeneic stem cell transplantation (aSCT). Specific antiviral therapy is only available for CMV. With the exception of ganciclovir all drugs are being used off-label. 40-50% of patients reactivate CMV following aSCT. For the 20-30% of patients reactivating EBV, only ritux...
Background: Quantification of CD34+ cells in peripheral blood stem cell apheresis is normally performed by single platform flow cytometric measurements according to the ISHAGE protocol. Peripheral blood stem cell concentrates (PBSC) produced by apheresis normally contain many T cells. Those T cells can be used for production of donor lymphocyte inf...
Background:
Neutrophil alloantibodies are well-known triggers of transfusion-related acute lung injury (TRALI) and also cause immune neutropenia. Alloimmune neutropenia due to transfusion is an isolated phenomenon that is only rarely identified. Its incidence is specified in the literature as being less than one in 10,000 transfused plasma-contain...
Recently, two prospective randomized multicenter studies on hypoproliferative thrombocytopenia compared prophylactic transfusion of platelet concentrates below a threshold of 10 000/μL with therapeutic platelet transfusions only, administered at onset of hemorrhages other than mucocutaneous bleeding (1, 2). In the patient group with only therapeuti...
Regulatory T cells (Tregs) and other T-cell subsets are of importance in the setting of autologous and allogeneic stem cell transplantations. We conducted a study to assess the content of peripheral blood stem cell concentrates and related apheresis parameters in the autologous and allogeneic setting.
We characterized 53 donors, patients and periph...
Background:
In Germany, cord blood needs to be transported to the processing facility to be processed and cryopreserved within 48 hours after collection according to national guidelines. During that time, a temperature of 22 ± 4 degrees C must be maintained. The purpose of this study was to analyse the influence of temperature during transport and...
Background and Objectives
Since 2001, all blood components in Germany must be leucocyte depleted. Recently, a new method for quality control of depletion was introduced. Our study aimed at the validation of the method for routine use in apheresis platelet concentrates.Materials and Methods
We compared the new ADAM-rWBC device with manual counting i...
The novel allele HLA-B*08:113 identified in two related individuals of Caucasian origin is described.
In addition to bone marrow or peripheral blood derived stem cells, cord blood (CB) is an alternative source for hematopoietic stem cells. This report shows the impact of higher concentrations of leukocytes, mononu- clear cells (MNCs), and CD34-positive cells on the viability of CB derived stem cells after cryopreservation.
Statistical analysis of d...
In most cases, the amount of hematopoietic stem and progenitor cells (HSPCs) in a single cord blood (CB) unit is not sufficient for allogenic transplantation of adults. Therefore, two CB units are usually required. The ex vivo expansion of HSPCs from CB in coculture with mesenchymal stroma cells (MSCs) might be an alternative. It was investigated,...
The novel allele HLA-DQB1*02:27 identified in two related individuals of Caucasian origin is described.
Background:
For cryopreservation of stem cells, a cryoprotective agent is essential. Dimethyl sulfoxide is commonly used, but has deleterious effects on cell vitality in the warmth and for the recipients of stem cells. The aim of the study was to reduce DMSO and find one cryoprotective solution suitable for stem cells of different origin. Material...
Background:
Quantification of CD34+ cells in peripheral blood stem cell apheresis is normally performed by single platform flow cytometric measurements according to the ISHAGE protocol. Peripheral blood stem cell concentrates (PBSC) produced by apheresis normally contain many T cells. Those T cells can be used for production of donor lymphocyte in...
4352
Background and objectives
We have recently shown that memory B-lymphocytes from murine CMV immune donor animals adoptively transferred into immunodeficient mice were highly effective in protecting from a viral infection indicating a therapeutic potential of virus specific memory B-cells. These preclinical data provided evidence that a cell-ba...
Background:
The objective was to investigate potential risks for apheresis donors associated with a triple-plateletpheresis (TP) program.
Study design and methods:
Eleven hemapheresis centers randomly assigned 411 repeat donors (ratio, 1:1.2) to either double plateletpheresis (DP; 185 donors) or TP (226 donors) with a platelet (PLT) target conte...
Antibodies against the human neutrophil antigens (HNA) are able to stimulate transfusion reactions, autoimmune and neonatal neutropenia. The aim of this study was to determine the HNA allele frequencies in the largest ethnic minority group in Germany in comparison with the German population for predicting the risk of alloimmunization and associated...
The novel allele HLA-B*07:68:02 identified in two related individuals of Caucasian origin is described.
Introduction: An increasing demand for blood components is opposed by a decreasing donor availability for the collection of the required blood components. Furthermore, current stem cell transplantation regiments require the collection of more than one similar or different component from one donor or patient. One strategy for maintaining the patient...
There exists only very few data on in vitro and in vivo effects of gamma irradiation of red blood cells (RBCs) that have been leukoreduced by filtration before a subsequent irradiation. Reported studies reflect neither the current Food and Drug Administration (FDA) nor the European recommendations on timing of irradiation and subsequent storage.
We...
Ovarian hyperstimulation syndrome is a potentially life-threatening complication during controlled ovarian stimulation for fertility treatment. Since no association of this condition with ABO blood groups was known, we compared ABO antigens with severity and onset of symptoms in a case-control study.
One hundred and twenty-one patients, mainly Cauc...
The standard mononuclear cell (MNC) program of the COM.TEC device (Fresenius HemoCare GmbH) showed excellent collection efficiency of CD14+ monocytes. A major disadvantage was high content of residual cells in MNC harvests, which could influence dendritic cell (DC) culture.
The autoMNC program (COM.TEC) was compared with the standard MNC program (n...
The aim of this study was to investigate the effect of gamma irradiation with 30 Gy on the coagulation system in leukoreduced fresh-frozen plasma (FFP).
In 74 FFP units that had been stored for 352 +/- 103 days below -30 degrees C, the following variables were determined in parallel in an irradiated and not irradiated half: prothrombin time (PT); a...
This prospective study compared white blood cell (WBC) storage in polyvinylchloride (PVC) bags and in polyolefin (POF) bags. After leukapheresis, CD14+ monocytes, CD11c+, and CD123+ precusor dendritic cells (DCs) were analyzed under platelet (PLT) storage conditions.
Twenty-five leukapheresis procedures were performed on blood cell separators (AS.T...
The Fresenius COM.TEC cell separator is a new device for producing white cell concentrates (WBCs) and leukoreduced single-donor platelet concentrates (SDPs) and performing therapeutic cytapheresis and plasmapheresis that might replace the Fresenius systems AS104 and AS.TEC 204. This novel system's performance was evaluated for producing leukoreduce...
To compare the performance of seven currently available test systems in the detection of erythrocyte alloantibodies (ab), we tested in parallel 446 sera samples containing red cell ab [368 sera samples with ab that are assumed to be clinically significant (cs-ab) and 78 sera samples with ab that are assumed to be of minor clinical significance (ms-...
The aim of this study was to investigate the occurrence of hypotension in the 24-hour period after preoperative autologous blood donation (PABD) in patients with and without hypertension.
In 20 patients, 24-hour ambulatory blood pressure monitoring (ABPM) was performed before PABD was started and on every donation day in two repeated phlebotomies....
Only a few systematic studies have examined the effect of variable produced small platelet (PLT) aliquots on PLT function before transfusion to neonates. Although neonatal transfusion could be critical, no standardization of production or systematic quality controls have been introduced so far.
PLT split products were prepared in three different wa...
Lowering the plasma content in single-donor platelet (PLT) concentrates well below 30% implies the need to collect platelets at very high concentrations. Trima Accel (TA) is validated for collection below 4000 x 10(3) PLTs/microl. We evaluated its performance at 5000 x 10(3) PLTs/microl.
Twenty blood donors underwent apheresis with TA twice collect...
Rare clinical conditions cause the need for washed platelet (PLT) concentrates (PCs). Saline-washed PCs can only be stored shortly, however, owing to lack of substrates for PLT metabolism. New PLT additive solutions (PASs) contain such substrates and might be used alternatively. The in vitro quality of apheresis PCs washed with Composol-PS or modif...
The COM.TEC cell separator (Fresenius Hemocare), equipped with the MNC program (single-stage chamber) and the PBSC-LYM program (dual-stage chamber), was evaluated for CD 14+ cell collection regarding cell yields, collection efficiencies (CEs), and the content of residual cells in harvests.
Twenty-four non-cytokine-stimulated donors underwent 5-L mo...
Platelets (PLTs) contain an assortment of growth factors (GFs), in particular PLT-derived GFs (PDGFs), transforming GFs (TGFs), and vascular endothelial GF (VEGF) (1). By topical release and action of these GFs simultaneously with thrombus formation and bleeding cessation, PLTs initiate the processes of wound repair, angiogenesis, and defense again...
New platelet (PLT) additive solutions (PASs) allow a plasma carryover of < 30% in PLT concentrates. This implicates the need to collect apheresis PLT concentrates at very high PLT concentrations: so-called dry PLTs (DPs). We used the TRIMA, with software version 4 (TRIMA V4), to collect such DPs and investigated the in vitro quality of these PLTs w...
Standard plateletpheresis techniques and effects on platelet (PLT) donors were investigated to provide an informative basis for advancement of apheresis software.
Three paired groups with 33 male and 22 female blood donors were prospectively investigated by analyzing blood counts of donors and products. Four apheresis platforms, the COBE Spectra LR...
We introduced monitoring of mononuclear cell (MNC) counts to obtain enhanced donor control and a stable quality of MNC products, because there are limited data available about blood donors after serial leukapheresis (LP) procedures.
In a prospective paired study, 13 male healthy blood donors underwent 10-L LP procedures performed on two apheresis d...
Recently, long-standing elevations of soluble growth factors released from platelets (PLTs) after contact with artificial surfaces during dialysis were described. They could be jointly responsible for the high frequency of death from cardiovascular diseases in dialysis patients. There are no comparable data on the extent and the duration of a growt...
No data are currently available on the quality of washed prestorage leucocyte-depleted red blood cell concentrates (RCCs).
Five groups of RCCs stored in additive solution (SAG-M) were washed. The groups differed in the age of RCCs (2-5 days or 11-15 days), the temperature during the washing procedure and a 6-h storage period (4 degrees C or room te...
In cancer and transplantation therapy apheresis devices and software of optimum standards are required for the collection of high cell yields with high purity of the desired cell fraction.
In a paired study, 15 healthy blood donors underwent four 10-L leukapheresis procedures (197 +/- 33 min) with an inlet blood flow rate of 60 mL per minute by use...
Einleitung: Maternale thrombophile Mutationen werden gehauft bei schweren Schwangerschaftkomplikationen (Habituelle Aborte, Praeklampsie, HELLP Syndrom, vorzeitige Plazentalosung und intrauterine Wachstumsverzogerung) gefunden. Bisher liegen nur wenige widerspruchliche Berichte uber die Assoziation fetaler Mutationen bei diesen Komplikationen und i...
Aside from new software the blood cell separator TRIMA (GambroBCT) also received a newly designed separation chamber offering a novel single stage separation technology, called Trima Accel. We evaluated this new system focusing on productivity and donor comfort by comparing it to the previous version (Trima version 4) in collecting single-donor pla...
This study was conducted to investigate the association of maternal and/or fetal factor V Leiden (FVL) and G20210A prothrombin mutation with HELLP syndrome. FVL and G20210A prothrombin mutation were determined using PCR. Sixty-three pregnant women, 36 of them diagnosed with HELLP syndrome, were included in the study. Overall, 68 children were born...
The properties of red blood cell (RBC) concentrates stored in different additive solutions have been previously examined under laboratory conditions at the end of shelf-life. However, whether these data are representative for RBC units used in clinical practice has not been shown. Therefore, we examined 164 RBC units from six manufacturers outdated...
A new cell separator (COM.TEC, Fresenius) was recently developed aimed at efficient collection of WBC-reduced single-donor PLT concentrates (SDPs).
Five German centers collected 554 WBC-reduced SDPs with help of the COM.TEC cell separator. Two multicenter cell counting studies were performed at the beginning and at the end of the study to document...
Monocytes collected by leukapheresis are increasingly used for dendritic cell (DC) culture in cell factories suitable for DC vaccination in cancer.
Using modified MNC programs on two apheresis systems (Cobe Spectra and Fresenius AS.TEC204), leukapheresis components collected from 84 patients with metastatic malignant melanoma and from 31 healthy ma...
Umbilical cord blood processing with standard centrifugation techniques is performed in open systems and results in varying cell and volume recoveries.
Forty umbilical cord blood donations were randomly assigned to processing either with a microprocessor-controlled cell separator equipped with closed disposables or with a manual separation procedur...
Life-threatening gastrointestinal hemorrhage in Crohn’s disease is rare but well recognized and represents about 1% of the cases for hospital admissions in Crohn’s disease. Patients with ileocolic location of disease are More likely to have severe hemorrhage and May require resection of the small bowel, which is the Most frequently performed intest...
BACKGROUND: A cell separator (Spectra, Gambro BCT) with an integrated leukoreduction system (LRS) for producing WBC-reduced single-donor platelet concentrates has been shown to result in a slightly reduced collection efficiency as compared to the former Spectra system without LRS. A novel modified system for improved collection efficiencies (LRS Tu...
To reduce the production costs of single-donor platelets (SDPs), a study was conducted to investigate whether plasma collected as a by-product of plateletpheresis satisfies the quality requirements for FFP without impairing the quality of the SDP component.
Ninety-two donors with platelet (PLT) counts <270 x 10(9) per L underwent plateletpheresis u...
BACKGROUND: Compensatory RBC production during repeated preoperative autologous blood donation (PABD) shows marked interindividual variability. This study was performed to reveal variables that might be useful to predict the amount of the erythropoietic response to PABD in an individual patient who was not iron deficient.
STUDY DESIGN AND METHODS:...
Purpose: The cell separator COM.TEC uses plasma recirculation in platelet (PLT) collection for obtaining high PLT yields and good purity of the product by standardizing the quality of the whole blood to be processed. Methods: PLT collections in six centers were evalutated. The donors underwent PLT collection with the COM.TEC (Fresenius. Bad Homburg...
We assessed autologous bone marrow (BM) harvest and hematologic recovery after high-dose chemotherapy (HDCT) in patients who failed to achieve peripheral blood stem cell (PBSC) mobilization. One hundred and ninety-three patients with germ cell tumor, malignant lymphoma, sarcoma or medulloblastoma were scheduled for HDCT. In 123 patients, PBSC were...
A new software version of a cell separator (AS TEC 204, Fresenius) providing WBC-reduced single-donor plateletpheresis concentrates was tested.
Dual-needle apheresis procedures (n = 621) were performed in three centers, using either fixed interface positioning (FIP) or periodic alternating interface positioning (PAIP). The other separation paramete...
The problem of how to deal with red blood cell concentrates (RBCs) prepared from under- or overcollected units of whole blood (WB) and how to collect blood from underweight persons arises in the context of autologous predeposit. To determine the quality of RBCs stored in PAGGS-M additive solution prepared from under- and overcollected units of whol...
The purpose of this study was to compare the performance of four currently available microtube column agglutination systems in the detection of red cell alloantibodies to that of the standard tube low-ionic-strength solution (LISS) indirect antiglobulin test (IAT) (tube LISS-IAT).
In a comparative study, 172 sera, previously demonstrated to contain...
Summary Background: The consequences of the new edition of the ‘German Guidelines for Blood Grouping and Blood Transfusion (Haemotherapy)’ for aptitude tests of haemapheresis donors were studied. Material and Methods:During a 19-month period the reasons for exclusion and deferral of first-time donors were retrospectively evaluated, and the resultin...
Background: The consequences of the new edition of the 'German Guidelines for Blood Grouping and Blood Transfusion (Haemotherapy)' for aptitude tests of haemapheresis donors were studied. Material and Methods: During a 19-month period the reasons for exclusion and deferral of first- time donors were retrospectively evaluated, and the resulting excl...
Megakaryocytopoiesis and platelet production are regulated by several hematopoietic growth factors. The present study focuses on the effects of automated plateletpheresis on systemic levels of different hematopoietic growth factors.
Platelet count, mean platelet volume, and serum levels of thrombopoietin, erythropoietin, interleukin-1beta, interleu...
Transfusions or pregnancies can cause immunization against private HLA determinants and public epitopes shared by more than one private HLA antigen. HLA antibodies are correlated with febrile transfusion reactions, lower platelet response following platelet transfusion, and an increased rate of renal transplant rejection. Until now, antibody specif...
This study was performed to evaluate the capacity of oral and intravenous (i.v.) iron administration during autologous blood donation (ABD) to improve the efficacy of ABD and to prevent the need for allogeneic blood transfusion in patients without iron deficiency who are undergoing major elective surgery for which a minimum of 3 autologous units ha...
Quality assurance of blood transfusion covers institutions, personnel, and procedures involved in preparing, issuing, and using blood components. The accuracy of data related to blood component transfusions is a tool for quality control in the transfusion service.
A study of the accuracy of data records of the transfusion service at the University...
It has been suggested that inflammatory cytokines such as Interleukin (IL)-1beta, IL-6, tumor necrosis factor-alpha (TNF-alpha) and IL-8 might be responsible for a large number of non-antibody-mediated adverse reactions to the transfusion of blood components, especially of platelet concentrates (PCs). The aim of this study was to compare the levels...
Purpose Computer software used in the transfusion service is a device which is an instrument for and a subject to quality control procedures. Methods The blood bank software system developed for our hospital-based blood transfusion service, BB-Data (Friedrich-Karl Zick, Berlin, Germany), is composed of program functions and several databases which...
Purpose: Febrile transfusion reactions and impaired increment following platelet transfusion may be associated with alloimmunization against HLA antigens. Antibody specificities toward public epitopes of class I HLA antigens have been poorly characterized in platelet recipients. Methods: We tested 9,060 consecutive serum screens from platelet recip...
Purpose: To evaluate the performance of four currently available microtube column systems in the detection of red cell alloantibodies in comparison to the standard tube spin low-ionic-strength solution indirect antiglobulin test (LISS-IAT). Methods: In a comparative study, we tested 172 sera which were previously demonstrated to contain red cell al...
Purpose: To assess the feasibility of hematopoietic rescue by BM in pts who failed to mobilize peripheral blood stem cells (PBSC). Patients and Methods: 193 pts suffering from germ cell tumor (n=156), malignant lymphoma (n=30), sarcoma (n=6) and medulloblastoma (n=1) with a median age of 32 years (10 to 59) were evaluated for high-dose chemotherapy...
Purpose: A high platelet contamination of mononuclear cell concentrates can impair the quality of cultured dendritic cells, due to cytokine production induced by the interaction of activated platelets with monocytes. Mononuclear cell concentrates obtained from healthy donors or patients with normal peripheral platelet count using standard MNC progr...
Citations
... In der Praxis werden aber meist nur einige HPA-Allele gleichzeitig untersucht, um die Sensitivität und Spezifität der Methode nicht zu überlasten. Diese Art der Typisierung wurde auch in der hier vorliegenden Dissertation und der daraus hervorgegangenen Publikation angewendet(Hauck-Dlimi et al., 2019). Andere, alternative Methoden zur HPA-Typisierung können hier nicht umfassend dargestellt werden, es seien daher nur einige wenige erwähnt. ...
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... When only DSA-positive unrelated donors are available, desensitization treatment is recommended to facilitate donor engraftment, as recently postulated by the EBMT consensus guidelines on DSAs [35]. At present, there is little evidence concerning desensitization therapy in patients with DSA undergoing unrelated donor HCT [39]. Most previous studies on desensitization have been performed in the haploidentical donor context, with either PTCy [34,40] or Beijing platforms [41]. ...
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... To test the translatability of our predictions, we used a GMP-compliant procedure to obtain antigen-specific T cells through peptide stimulation of leukapheresis products. 30 We prepared peptide mixtures such that each tier of twenty peptides was split into four pools of up to five peptides, yielding a total of 12 peptide pools, to curtail experimental demand. In the HE tier, peptides were assigned to the pools in descending order of ES. ...
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... Frequency of FCGR3B*02 was comparable to Chinese and Thais but was higher than Japanese and lower than Caucasians, Germans, Brazilians, and Turkish [22, 26, 30, 33, 35-37, 39, 42]. Frequency of FCGR3B*03 (8.76%) was highest as compared to all other populations [22,[25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44] except Zambians. This may be due to the ethnic differences among both population groups and/or sample size variation. ...
... Neben FNAIT und Purpura kann es durch Alloimmunreaktionen gegen Lymphocyte Antigens" (Strobel et al., 2014;Strobel et al., 2018;Zingsem et al., 2018) vor allem die HPAs oder "Human Platelet Antigens" verantwortlich. Daher ist auch bei der Purpura, ähnlich wie bei der zuvor beschriebenen FNAIT, die Bildung von Alloantikörpern gegen HLA-Antigene die Ursache, vor allem gegen HPA-1, -2 und -5 (Eyada et al., 2018). ...
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... Neben FNAIT und Purpura kann es durch Alloimmunreaktionen gegen Lymphocyte Antigens" (Strobel et al., 2014;Strobel et al., 2018;Zingsem et al., 2018) vor allem die HPAs oder "Human Platelet Antigens" verantwortlich. Daher ist auch bei der Purpura, ähnlich wie bei der zuvor beschriebenen FNAIT, die Bildung von Alloantikörpern gegen HLA-Antigene die Ursache, vor allem gegen HPA-1, -2 und -5 (Eyada et al., 2018). ...
... Performance evaluations of these instruments have shown excellent correlations and good agreement between the hematopoietic stem cell counts obtained using a bead-based approach and those obtained using volumetric counting. 37,38 In addition, the reliability of the Accuri instrument has been reported in platelet counting. 39 Although standard flow cytometry is a widely used approach, it is limited when measuring the small size of the MPs shed. ...
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... Finally we wanted to combine the quantification of both, stem cells and further cell populations. Strobel et al. combined the ISHAGE protocol with a CD3 determination using a liquid reagent panel, whereby the bead-based quantification of the CD3 determination performed better than the volumetric quantification on the flow cytometer 22 . In addition to the stem cell and T cell population, the pre-formulated dried reagent tube used in our approach allows to determine the B cells with the help of bead counts. ...
... Fry et al. dagegen empfehlen eine Konzentration von 7,5 -10 % DMSO (Fry et al. 2015). In Bezug auf das optimale Kryoprotektivum ist jedoch das letzte Wort noch nicht gesprochen (Strobel et al. 2017 ...
... Eine weitere Steigerung der Granulozytenausbeute (ca. 4 × 10 10 ) wurde durch Stimulierung der Granulozytopoese mit G-CSF möglich. Spender sollen nicht häufiger als viermal jährlich Granulozyten spenden [25]. Granulozytenkonzentrate sind unmittelbar nach der Herstellung zu transfundieren. ...
Reference: Transfusionsmedizin und Immunhämatologie
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