B.M. Lange's research while affiliated with Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) and other places
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Publications (5)
Suspension cultures of Picea abies (L.) Karst released polymeric material into the culture medium when treated with an elicitor preparation from the spruce needle pathogen Rhizosphaera kalkhoffii. The presence of lignin (about 35%, w/w) was demonstrated by phloroglucinol/HCI reactivity and quantitation with thioglycolic acid. Carbohydrate (about 14...
Treatment of Pinus sylvestris L. cell-suspension cultures with an elicitor preparation from the pine needle pathogen Lophodermium seditiosum, resulted in a severalhundredto thousandfold accumulation of the stilbenes pinosylvin and pinosylvin 3-O-methyl ether in methanolic cell extracts. There was a simultaneous induction of the biosynthetic enzymes...
Citations
... The total chlorophyll content was determined as described previously ( . Lignin was measured quantitatively according to Lange et al. (1995). Extraction of cell wall materials and determination of cellulose contents were conducted as described previously (Updegraff, 1969;Li et al., 2003). ...
... Recently, Kim et al. (2022) reported that PME and DPME accumulation was highly increased in calli of P. koraiensis when the callus was treated with fungusderived elicitors. In the cell culture of P. sylvestris, treatment with fungal mycelium extracts from Lophodermium seditiosum strongly increased the accumulation of PME hundreds of times (Lange et al. 1994). It is questioned whether fungal elicitor treatment increases PME and DPME accumulation in P. strobus cells and what kinds of TFs are involved in the accumulation of PME and DPME in P. strobus cells by fungal elicitor treatment. ...
... Lignin quantification was performed using an acid-catalysed reaction that forms soluble lignin-thioglycolate complexes. Suitable for photometric measurements, this method was developed in spruce (Picea abies; Lange et al., 1995), but later adapted for use in N. benthamiana (de la Torre et al., 2014). For each sample, 200 mg of grounded biomass was used. ...