Akira Sakai's scientific contributions
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Publications (2)
Embryogenic cells of asparagus (Asparagus officinalis L.) were successfully cryopreserved by vitrification and subsequently regenerated plants. The cells were cryoprotected with a mixture of 2 M glycerol and 0.4 M sucrose at 25°C for 10 min and then transferred to 1.8-ml plastic cryotubes. The cryoprotected cells were dehydrated with a new vitrific...
Citations
... Germination rate and germination speed index (GSI) were calculated according Embryos were immersed in either PVS2 or PVS3 cryoprotectant solutions at 4 o C for one hour and then either placed in Al foil packets (no solution) or in cryovials (1.8 mm -Nunc, Roskilde, Denmark) containing 1.5mL of PVS2 or PVS3. The composition of PVS2 was (30% glycerol w/v, 15% w/v dimethylsulfoxide, 15% w/v ethylene glycol and 13.7% w/v sucrose) (Sakai et al. 1990) and PVS3 was (50% w/v sucrose and 50% w/v glycerol) (Nishizawa et al. 1993). The other treatment used four embryos (0.13 g H 2 O.gDW − 1 ) placed in an Al foil packet. ...
... The application of a vitrification solution (PVS -Plant Vitrification Solution) to explants is one of the most significant advancements in this field and involves the use of a mixture of cryoprotectants (ENGELMANN, 2011). The most common solutions for plant species are PVS2, composed of 30% glycerol, 15% ethylene glycol, 15% DMSO, and 0.4 M sucrose, and PVS3, composed of 50% glycerol and 50% sucrose, optionally in liquid MS medium (NISHIZAWA et al., 1993). ...
