Fecundity of Metamysidopsis elongata (Crustacea: Mysidae) from Mazatlán Bay, Sinaloa, Mexico

Abstract

Background:
In any culturing of organisms, to calculate fecundity we need to know how many descendants are produced.

Goals
Marsupial and post-marsupial fecundity was calculated for Metamysidopsis elongata (Holmes, 1900) in two culturing generations (F1 and F2), and wild caught from Mazatlán Bay.

Methods
From September 2010 to October 2011, we carried out monthly sampling on foot with a plankton net with a mesh size of 1 000 µm and a mouth opening of 50 cm in diameter. The fecundity evaluation was calculated with organisms fixed with formaldehyde at 4%. Culturing occurred at a density of 5 ind • L⁻¹ at 22 ± 1 ºC, salinity 32 ‰ with continuous aeration. The mysids were fed ad libitum with recently hatched Artemia nauplii.

Results
The average marsupial fecundity was 6.28 ± 2.94 embryos per female in wild mysids, 4.51 ± 1.20 in the F1, and 3.72 ± 0.89 in the F2. The average post-marsupial fecundity was 5.86 ± 1.45 in wild mysids, 3.74 ± 1.20 in the F1, and 3.32 ± 1.42 in the F2. No correlation between the length of females and fecundity was found.

Conclusions
The fecundity of the wild mysids was present throughout the sampling cycle and was lower than that of the cultivated ones.

Full text

Vol. 28 No. 1 • 2018
Fecundity of Metamysidopsis elongata (Crustacea: Mysidae) from Mazatlán Bay, Sinaloa, Mexico
Fecundidad de Metamysidopsis elongata (Crustacea: Mysidae) de la Bahía de Mazatlán, Sinaloa, México
Armando A. Ortega-Salas, Judith Nuñez, Sergio Rendón and Arturo Nuñez†
Instituto de Ciencias del Mar y Limnología y Posgrado, Universidad Nacional Autónoma de México. Calzada Joel M. Camarena s/n, Mazatlán, Sinaloa, 82040. México
e-mail: ortsal@cmarl.unam.mx
Recibido: 09 de noviembre de 2017. Aceptado: 28 de enero de 2018.
Ortega-Salas A. A., J. Nuñez, S. Rendón and A. Nuñez†. 2018. Fecundity of Metamysidopsis elongata (Crustacea: Mysidae) from Mazatlán Bay, Sinaloa, Mexico.
Hidrobiológica 28 (1): 103-108.
ABSTRACT
Background. In any culturing of organisms, to calculate fecundity we need to know how many descendants are produ-
ced. Goals. Marsupial and post-marsupial fecundity was calculated for Metamysidopsis elongata (Holmes, 1900) in two
culturing generations (F1 and F2), and wild caught from Mazatlán Bay. Methods. From September 2010 to October 2011,
we carried out monthly sampling on foot with a plankton net with a mesh size of 1 000 µm and a mouth opening of 50 cm
in diameter. The fecundity evaluation was calculated with organisms xed with formaldehyde at 4%. Culturing occurred at
a density of 5 ind • L-1 at 22 ± 1 o C, salinity 32 ‰ with continuous aeration. The mysids were fed ad libitum with recently
hatched Artemia nauplii. Results. The average marsupial fecundity was 6.28 ± 2.94 embryos per female in wild mysids,
4.51 ± 1.20 in the F1, and 3.72 ± 0.89 in the F2. The average post-marsupial fecundity was 5.86 ± 1.45 in wild mysids,
3.74 ± 1.20 in the F1, and 3.32 ± 1.42 in the F2. No correlation between the length of females and fecundity was found.
Conclusions. The fecundity of the wild mysids was present throughout the sampling cycle and was lower than that of the
cultivated ones.
Keywords: culturing, fecundity, marsupial, Metamysipdopsis elongata, post-marsupial
RESUMEN
Antecedentes. En cualquier cultivo de organismos, el cálculo de la fecundidad es esencial para saber cuántos descen-
dientes podríamos tener para planear nuestro trabajo. Objetivos. Se calculó la fecundidad marsupial y post-marsupial
para Metamysidopsis elongata (Holmes,1900) en dos generaciones de cultivo (F1 y F2) y silvestres capturados en la
Bahía de Mazatlán. Métodos. Se realizaron muestreos mensuales a pie con una red de plancton de 1 000 μm de tamaño
de malla con apertura de boca de 50 cm de diámetro entre septiembre de 2010 y octubre de 2011. La evaluación de la
fecundidad se realizó con organismos jados con formol al 4% Los cultivos tuvieron una densidad de 5 ind • L-1 a 22 ±
1 o C, salinidad 32 ‰ con aireación continua. Los mísidos fueron alimentados ad libitum con náuplios recién nacidos de
Artemia. Resultados. La fecundidad marsupial media fue de 6.28 ± 2.94 embriones por hembra en mísidos silvestres,
4.51 ± 1.20 en el F1 y 3.72 ± 0.89 en el F2. La fecundidad post-marsupial media fue de 5.86 ± 1.45 en mísidos silves-
tres, 3.74 ± 1.20 en la F1 y 3.32 ± 1.42 en la F2. No hubo correlación entre la longitud de las hembras y la fecundidad.
Conclusiones. La fecundidad de los mísidos silvestres estuvo presente a lo largo del ciclo muestreado y fue menor que
la de los mísidos cultivados.
Palabras clave: cultivo, fecundidad, marsupial, Metamysipdopsis elongata, post-marsupial
Hidrobiológica 2018, 28 (1): 103-108

104 Ortega-Salas A. A. et al.
Hidrobiológica
INTRODUCTION
Mysids species have a short reproductive cycle, which means they can
quickly reproduce in vast numbers, and are a potentially useful food
source for both wild and cultured organisms (Biju et al., 2009). In all
species in the order Mysida, the females carry their embryos in the
brood pouch or marsupium, a subthoracic chamber formed by pairs
of overlapping oostegites, where juveniles develop until they attain the
adult form (Price, 2004).
Metamysidopsis elongata (Holmes, 1900) has two currently recog-
nized subspecies, the nominal subspecies M. e. elongata from the Pa-
cic Ocean and M. e. atlantica (Băcescu, 1968) from the Atlantic Ocean
(Băcescu, 1968).
Copulation, lasting a few seconds, occurs at night 2 or 3 minutes
after the mature female has molted (Mauchline, 1980). Eggs are ejec-
ted from the external genital opening of the oviduct near the base of
the sixth pair of pereiopods and the male sperm mass is placed in the
pouch. The embryos (fertilized eggs) are incubated within the pouch
until rst larval ecdysis. The juveniles are released shortly thereafter,
at night and over a short period of time, from minutes to an hour. After
release, juveniles tend to sink and undergo a second larval ecdysis;
statocysts appear and they are able to swim, acquiring their highly mo-
bile juvenile form in a few minutes after release (Murano, 1999; and
Mauchline, 1980).
Males and females develop distinct morphological features
during the period of rapid growth before maturity (after maturity,
growth becomes slower). In males, the four pleopods reach the trai-
ling edge of the last abdominal segment, and the male lobe is fu-
lly developed and setose. Females have elongated oostegites (the
marsupium growing) (Murano, 1999). However, Nair (1939) repor-
ted dimorphism in the rst abdominal appendages and antennu-
les, even though there is no development of the pouch and penis.
After the release of juveniles, the female begins the molting cycle, co-
pulation, embryos are developed, and release of juveniles again (Mura-
no, 1999). The studies by Ortega-Salas et al. (2008) of the fecundity of
Mysidopsis californica (W. M. Tattersall, 1932) from Mazatlán Bay un-
der semi-controlled conditions showed a low correlation (r = 0.27, p =
0.196) between the number of released juveniles and female length. In
addition, Rendon-Valdez (2013) studied some aspects of the reproduc-
tive biology of the mysid Amathimysis trigibba (Murano & Chess, 1987)
in natural conditions in Mazatlán Bay. Calil & Borzone (2008) mentioned
that tropical mysids, such as Metamysidopsis neritica (Bond-Buckup
& Tavares, 1992), reproduce continually in latitudes less than 40º N.
Ortega-Salas et al. (2015) studied growth and survival in M. elongate
they grew. The average growth rates by the von Bertalanffy model for
juvenile male and female mysids were 0.304 mm day-1, 0.149 mm day-1
and 0.208 mm day-1, respectively.
Fecundity is an important biological parameter because it indicates
reproductive potential and is measured by the number of eggs, embr-
yos, or larvae that females have in different sizes (Clutter & Theilacker,
1971; Nath, 1973; Nuñez-Lecuanda, 2013). Also, data on fecundity are
important in calculating the size of a stock, so the marsupial and post-
marsupial fecundity of M. elongata in Mazatlán Bay was calculated un-
der wild conditions and in two culture generations.
MATERIALS AND METHODS
On Mexico’s Pacic coast in Mazatlán Bay, a surface sample of 250-300
mysids was collected manually each month from September 2010 to
October 2011 over the sandy areas with a plankton net (mesh 1000
μm, mouth opening 50 cm diameter). The water temperature and sa-
linity were recorded for each sampling date and the organisms were
transferred to the laboratory. Acclimation lasted three days. Transparent
bottles (4 L) were used at a seawater temperature of 22 ± 1° C, salinity
32 ± 1, photoperiod 14:10 (light: dark) provided by 40W uorescent
tubes and constant-soft aeration air-stones. Every day organisms were
fed Artemia (aged 18-48 h and average length 480 μm) ad libitum;
brand-INVE Aquaculture-Artemia Systems, grade A with a hatching rate
of 100 000 nauplii per gram dry cyst hatched under intense uorescent
light tube at 24 °C and salinity between 33 and 35. Fifty percent of
the water was replaced every two days. Males were distinguished by
extended fourth pleopods, a lobe with hair-like setae, and the presence
of antennae; females had elongated oostegites sufcient in size to form
a pouch full size.
In 200 mysids of the wild population, marsupial stages of deve-
lopment were identied and described by Nuñez-Lecuanda (2013).
Frequency of development stages determines the marsupial fecundity
(number of embryos). A dilute menthol solution was applied to slowly
numb their bodies, and after 10-15 minutes they were xed with a
solution of 4% formaldehyde for 5-10 minutes. The marsupial fecun-
dity was determined by opening with a dissecting needle the pouch of
each ovigerous female whose pouch showed no signs of damage. The
terminology of Wortham-Neal and Price (2002) in Americamysis bahia
(Molenock, 1969) was used for the description of embryonic stages. For
the F1 and F2, the experimental design consisted of three 20 L aquaria
with 100 newly released young mysids each (density of 5 ind • L-1).
The average and standard deviations of the data were processed
with the Excel statistical package. Statistical analysis used StatSoftMR.
Normality was tested (Kolmogorov-Smirnov with Lilliefors p <0.05) and
then the nonparametric Kruskal-Wallis test was used.
To compare the proportions of ovigerous females and females with
an empty pouch, a nonparametric Mann-Whitney U test was used. Sig-
nicance was xed at p <0.05. A correlation analysis between female
length and number of embryos born was made.
RESULTS
In wild female mysids (n = 1 400), the marsupium contained 6.28 ±
2.94 embryos (range 1-18). Females with 4-7 embryos accounted for
55% of the population (Fig. 1, Table I). Average marsupial fecundity va-
ried signicantly with time (H = 259.5; p <0.05), being highest (9.7
± 2.32) in September 2010 and lowest (4.08 ± 1.48) in August 2011
(Fig. 2). 86% of the females sampled had an average fecundity of 6.17
± 2.84 embryos. Of the F1 females, 83% were carrying 4-6 embryos,
whereas of the F2 females 96% were carrying 3-5 embryos (Fig. 3).
Average marsupial fecundities of females F1 (4.51 ± 1.20) and F2
(3.72 ± 0.89) generations were signicantly lower (H = 55.3; p <0.05,
Tukey’s HSD) than in the wild females (avg. 6.28 ± 2.94), but they did
not differ from each other. The wild females were signicantly longer
than those of the F1 and F2. Table II summarizes average fecundity

105
Fecundity of Metamysidopsis elongata
Vol. 28 No. 1 • 2018
ranges (SD) and the length (range, average, and SD) of wild, F1 and
F2 females.
Average post-marsupial fecundity was 5.86 ± 1.45 in wild females
(n = 95), 3.74 ± 1.20 in F1 females (n = 50), and 3.32 ± 1.42 in F2
females (n = 50) (Fig. 4).
The fecundity of wild post-marsupial mysids was signicantly hig-
her than that recorded in the cultures (Table 2, Fig. 4) (Nuñez-Lecuanda,
2013). The lengths of the wild, F1, and F2 females used in the evalua-
tion of post-marsupial fecundity were not signicantly different (ANOVA
F1, 2,192 = 3.04, p >0.05). There was low correlation between fecun-
dity and length in post-marsupial wild females (r = 0.05, p >0.05), and
this also prevailed in F1 and F2 (r = 0.33 and r = 0.04, p >0.05 in both
cases). Within each generation there was a relationship between the
number of embryos and the length of the female: In the wild females,
the number of embryos increased at a rate of 1.10 embryos per 1 mm
increase in female length; in the F1 this increase was 0.76 embryos,
and in the F2 it was 0.61.

Figure 1. Frequency of fecundity in the marsupial sampling cycle of Metamysidopsis elongata (Holmes, 1900) from Mazatlán Bay, Sinaloa, Mexico.
Figure 2. Marsupial fecundity of Metamysidopsis elongata (Holmes, 1900) during sampling cycle from Mazatlán Bay, Sinaloa, Mexico.

Number of embryos/female
Months
95% Conf. int.
Stamd errpr
Mean
16
14
12
10
8
6
4
2
0
11
10
9
8
7
6
5
4
3
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
Sep 10 Nov Jan 11 Mar May Jul Sep
Frequency (%)
No of embryos

106 Ortega-Salas A. A. et al.
Hidrobiológica
DISCUSSION
Our observation of ovigerous females at 18-20 days was similar to the
25 days reported for M. e. atlantica (Gama et al., 2002) and (Gama et
al., 2006).
Fecundity, an important part of reproductive potential, is measured
by the number of eggs, embryos, and larvae with females at different
sizes (Clutter & Theilacker, 1971; Nath, 1973). In general, temperate
mysids produce between two to three generations per year (Mees et al.,
1994), whereas in the tropics they reproduce continuously (Goodbody,
1965). This was observed in M. elongata and Acanthomysis thailandica
(Murano, 1986) as well as in other tropical organisms such as Meso-
podopsis orientalis (W. Tattersall, 1908) (Hanamura, 2008; Biju et al.,
2009; Biju & Panampunnayil, 2010).
The temperature in the tropics where M. elongate is living is higher
than other species and they reproduce continuously. During summer
(22 °C), an average of 8.53 ± 0.18 embryos per female has been re-
corded for Mesopodopsis slabberi (Van Beneden, 1861), 42.65 ± 1.43
for Gastrosaccus spinifer (Goës, 1864), 16.04 ± 0.06 for Schistomysis
kervillei (G. O. Sars, 1885), and 6.09 ± 0.35 for S. spiritus (Norman,
1860), while in M. elongata the average marsupial fecundity was
6.28 ± 2.94 embryos per female in wild mysids, 4.51 ± 1.20 in the
F1 and 3.72 ± 0.89 in the F2. The average post-marsupial fecundity
was 5.86±1.45 in wild mysids, 3.74 ± 1.20 in the F1, and 3.32 ± 1.42
in the F2 at temperature of 22 ± 1 °C, salinity 32 ± 1, photoperiod
14:10 (light: dark). The wild mísidos eat great variety of food, whereas
the cultivated ones, only Artemia nauplii, this can be reected in the
number of offspring.
The relationship in M. elongate (6-8 mm) between the number
of embryos and the lengths of wild progenitors females, F1, and F2
showed a rate of 1.10, F1 0.76, and F2 0.61 embryos for each unit of
female length increase, respectively; these proportions were less than
the ones found in in A. trigibba, a species of smaller size (2.76 mm)
increase in length with 1.91 embryos / mm (Rendón-Valdez, 2013) and
M. californica (5.56 mm) increase in length with 1.56 embryos / mm
(Ortega-Salas et al., 2008). The giant mysid Gnathophausia ingens (Do-
hrn, 1870), the lophogastrid mysid (151 mm), has 1.0-2.31 embryos
per 1 mm increase in the length of the female (Childress & Price, 1978).
Mauchline (1980) stated that mysid fecundity is directly proportio-
nal to the total length of the females, and that it is an inverse function
of the metabolic rate, which is directly proportional to the water tempe-
rature. In M. elongata from Mazatlán Bay, the number of embryos was
low correlated with body length of females during the sampling cycle,
whether in wild-caught females, or in the F1, or F2 (r = 0.04, r = 0.005,
and r = 0.04; p <0.05, respectively). In A. thailandica, the maximum
number of wild embryos recorded was 18 in a female of 5.51 mm, but
in other females the same size, minor and major size, the number of
embryos in Stage I was 9, i.e., the same length females have different
numbers of embryos (Ramarn, 2012).
Table 1. Embryo number (range, mean±, SD) in wild Metamysidopsis
elongata (Holmes, 1900), and in cultured F1 and F2 at each develop-
ment stage from Mazatlán Bay, Sinaloa, Mexico.
Wild F1 F2
Stage I 1-15
(5.76±2.72)
(n=355)
2-6
(4.45±1.21)
(n=11)
2-5
(3.75±0.96)
(n=12)
Stage II 1-18
(6.41±2.87)
(n=322)
3-6
(4.0±1.41)
(n=5)
2-5
(3.27±1.0)
(n=11)
Stage III 1-14
(6.34±2.74)
(n=319)
4-6
(4.5±1.0)
(n=4)
3-5
(4.0±0.7)
(n=9)
Stage IV 1-16
(6.31±3.18)
(n=420)
3-7
(4.81±1.25)
(n=11)
3-5
(3.91±0.79)
(n=12)
Figure 3. Frequencies of marsupial fecundities among F1 and F2 females of Metamysidopsis elongata (Holmes, 1900) from Mazatlán Bay, Sinaloa, Mexico.

Number of embryos/female
F1
F2
1 2 3 4 5 6 7
40
35
30
25
20
15
10
5
0
Frequency (%)

107
Fecundity of Metamysidopsis elongata
Vol. 28 No. 1 • 2018

REFERENCES
Băcescu, M. 1968. Etude des quelques Leptomysini (Crustacea Mysida-
cea) des eaux Du Bresil et de Cuba: description d’un genre et de
cinq autres taxons nouveaux. Annali Museo Civico di Storia Natura-
le Genova 77: 232–249.
Biju, A., R. GiReesh, K. j. jAyAlAKshMi, c. K. hARidevi & s. u. PAnAMPunnAyil.
2009. Seasonal abundance, ecology, reproductive biology and bio-
chemical composition of Mesopodopsis orientalis. W. M. Tattersall
(Mysida) from a tropical estuary, Cochin backwater-India. Crusta-
ceana 82: 981-996. DOI:10.1163/156854009X448835
Biju, A. & s. u. PAnAMPunnAyil. 2010. Seasonality, reproductive biology
and ecology of Mesopodopsis zeylanica Crustacea: Mysida) from a
tropical estuary (Cochin backwater) in India. Plankton and Benthos
Research 5: 49-55.
cAlil, P. & c. A. BoRzone. 2008. Population structure and reproductive
biology of Metamysidopsis neritica (Crustacea: Mysidacea) in a
sand beach in south Brazil. Revista Brasileira de Zoologia 25 (3):
403-412. DOI:10.1590/S0101-81752008000300004
childRess, j. j. & M. h. PRice. 1978. Growth rate of the bathypelagic
crustacean Gnathophausia ingens (Mysidacea: Lophogastridae). I.
Dimensional growth and population structure. Marine Biology 50:
47-62.
clutteR, R. i. & G. h. theilAcKeR. 1971. Ecological Efciency of a pelagic
mysid shrimp; estimates from growth, energy budget and mortality
studies. Fisheries Bulletin 69: 93-115.
GAMA, A. M. s, M. A. Montu & l. F. M. GusMAo. 2002. Ciclo de vida pos-
marsupial e crescimento de Metamysidopsis elongata atlántica
(Crustacea, Mysidacea, Mysidae) em cultivo de laboratorio. Iherin-
gia, Série Zoologia, Porto Alegre 92 (1): 77-83.
GAMA, A. M. s, M. A. Montu & F. d’incAo. 2006. Ciclo de mudas e taxas
de crescimento de Metamysidopsis elongata atlántica (Crustacea:
Table 2. Length (mm), marsupial and post-marsupial fecundity of Me-
tamysidopsis elongata (Holmes, 1900) (interval, average, SD) of wild
females, F1 and F2 from Mazatlán Bay, Sinaloa, Mexico.
Marsupial Wild F1 F2
Female length
(mm)
4.24-6.98
(5.67±0.54)
5.28-6.52
(5.95±0.42)
5.36-6.62
(6.08±0.37)
Embryos number 1-18
(6.28±2.94)
(n=1 400)
2-7
(4.51±1.20)
(n=31)
2-5
(3.72±0.89)
(n=44)
Post-marsupial
Female length
(mm)
4.19-6.66
(5.35±0.74)
(n=250)
4.43-6.53
(5.41±0.60)
(n=50)
4.82-5.85
(5.36±0.32)
(n=50
Juveniles released 3-9
(5.86±1.45)
(n=95)
1-6
(3.74±1.20)
(n=50)
3-8
(3.32±1.42)
(n=50)
Juveniles length
(mm)
1.14-1.30
(1.24±0.03)
(n=150)
1.14-1.28
(1.20±0.03)
(n=150)
1.14-1.29
(1.20±0.04)
(n=271)
Figure 4. Frequencies of post-marsupial fecundities of wild, F1, and F2 females of Metamysidopsis elongata (Holmes, 1900) from Mazatlán Bay, Sinaloa, Mexico.
The fecundity of the wild mysids was present throughout the sam-
pling cycle and was lower than that of the cultivated mysids.
Fecundity of Metamysidopsis elongate is essential for knowing
how many descendants we could have to plan our work, but also to
establish the marsupial and post-marsupial fecundity in the wild and
in two culturing generations (F1 and F2). The continuous fecundity in
the wild mysids was present throughout the sampling cycle, although
it was lower in the cultivated ones; the ones in the wild ate a variety of
organisms, while the cultivated ones ate just Artemia nauplii.
Number of juveniles/female
Wild
F1
F2
1 2 3 4 5 6 7 8 9 10
45
40
35
30
25
20
15
10
5
0
Frequency

108 Ortega-Salas A. A. et al.
Hidrobiológica
Mysidacea) cultivado em diferentes temperaturas e salinidades.
Iheringia, Série Zoologia, Porto Alegre 96 (1): 67-70.
GoodBody, i. 1965. Continuous breeding in populations of two tropical
crustaceans, Mysidium columbiae (Zimmer) and Emerita portori-
censis Schmidt. Ecology 46: 195-197. DOI:10.2307/1935274
hAnAMuRA, y., R. siow & c. PhAiK-eAn. 2008. Reproductive Biology and
Seasonality of the Indo-Australasian mysid Mesopodopsis orienta-
lis (Crustacea: Mysida) in a tropical mangrove estuary, Malaysia,
Estuarine. Coastal and Shelf Science 77: 467-474.
MAuchline, j. 1980. The Biology of Mysids and Euphausiids. Advances in
Marine Biology 18: 681.
Mees, j., z. ABdulKeRiM & o. hAMeRlyncK. 1994. Life history, growth and
production of Neomysis integer in the Westerschelde estuary (SW
Netherlands). Marine Ecology Progress Series 109: 43-57.
MuRAno, M. 1999. Mysidacea. In: D. Boltovskoy (Ed). South Atlantic Zoo-
plankton. Leiden, Backhuys Publishers. pp. 1099-1140.
nAiR, K. B. 1939. The reproduction, oogenesis and development of Me-
sopodopsis orientalis Tatt. Proceedings of the Indian Academy of
Sciences, Sect. B 9 4: 175-223.
nAth, c. n. 1973. Breeding and fecundity in a subterranean mysid, Le-
pidomysis longipes (Pillai and Mariamma). International Journal of
Speleology. 5: 319-323.
neAl, j. l. & w. w. PRice. 2002. Marsupial developmental stages in Ame-
ricamysis bahia (Mysida: Mysidae). Journal of Crustacean Biology
22: 98-112.
nuñez-lecuAndA, j. 2013. Evaluación de la fecundidad marsupial y pos-
marsupial de Metamysidopsis elongata (Holmes, 1900) (Crustacea:
Mysidae) en condiciones silvestres y semicontroladas de la costa
de la Bahía de Mazatlán, Sinaloa México [Estimate of marsupial
and post-marsupial fecundity of Metamysidopsis elongata (Holmes,
1900) (Crustacea: Mysidae) from the shore of the Mazatlán Bay,
Sinaloa, México, under wild and semi-controlled conditions]. In:
postgraduate thesis. 67 p. + gs. (Universidad Nacional Autónoma
de Mexico, Mazatlan). Abstract available at http://132.248.246.25/
tesigrado/consulta/detpaterno.cfm?al_cuenta=511014697
oRteGA-sAlAs, A. A., A. núñez-PAstén & h. A. cAMAcho. 2008. Fecundity of
the crustracean Mysidopsis californica (Mysida, Mysidae) under
semi-controlled conditions. Revista de Biología Tropical 56: 535-
539. DOI:10.6024/jmbai.2015.57.2.1866-15
oRteGA-sAlAs, A. A., j. nuñez-lecuAndA, s. Rendón R. & A. nuñez P. 2015.
Growth and survival of Metamysidopsis elongata (Crustacea: My-
sidae) from Mazatlán Bay, Sinaloa, Mexico. Journal of the Marine
Biological Association of India 57 (2): 105-108. DOI:10.6024/jm-
bai.2015.57.2.1866-15
RAMARn, t., v. c. chonG & y. hAnAMuRA. 2012. Population structure and re-
production of the mysid shrimp Acanthomysis thailandica (Crusta-
cea: Mysidae) in a Tropical Mangrove Estuary, Malaysia, Zoological
Studies 51: 768:782.
RAPPe, K., n. FocKedey, c. v. colen, A. cAttRijsse, j. Mees & M. vincx. 2011.
Spatial distribution and general population characteristics of my-
sids shrimps in the Westerschelde estuary (SW Netherlands). Es-
tuarine, Coastal and Shelf Science 91: 187-197.
Rendón-vAldez, i. 2013. Algunos aspectos de la biología reproductiva de
Amathimysis trigibba (Crustacea:Mysida). Tesis de Licenciatura.
Facultad de Ciencias del Mar, Universidad Autónoma de Sinaloa.