Study polymorphism of ovine IGFBP-3 Gene and its association with carcass traits and fatty acids profiles in Zel sheep using PCR-SSCP

Abstract

IGF-1 hormone transports by connecting to IGF-binding proteins (often IGFBP-3) in the biological fluids. This protein acts as an IGF1 carrier and helps to prolong half-life of hormone and can increase or decrease biological activity of hormone on cellular surface. The purpose of the research was to study the polymorphisms of IGFBP-3 gene and its association with carcass traits and fatty acids profiles in Zel sheep as a thin-tailed breed using PCR-SSCP. In this study we used 130 Zel sheep randomly and recorded their traits measure we wanted. to determine of fatty acids, we took 6ml blood in venojects containing EDTA and immediately centrifuged them for 10 minutes by 3000g (5200 rpm) to separate the plasma. DNA was extracted from muscle tissue by Biotech company kit. Then the Polymerase chain reaction (PCR) used to amplify of 654 bp fragment of exon 3 of IGFBP-3 gene. PCR products were electrophoresed on polyacrylamide gel (method SSCP) and stained with silver nitrate method to distinguish different patterns. Results indicated the different patterns, may be due to polymorphism in this fragment. Frequency of patterns 1, 2, 3, 4 and 5 were 67/66, 33/3, 67/6, 33/13 and 10 percent respectively. Trait carcass weight (P <0.05), back fat thickness (P <0.01) and crude protein in meat (P <0.01) had significantly differences with different patterns. Significant association observed by Heptadecanoic acid (C17: 0) with different patterns of IGFBP3 gene. No other association was observed by IGFBP3 patterns with other traits and fatty acids profiles.

Full text

يزروﺎﺸﻛ يژﻮﻟﻮﻨﻜﺗﻮﻴﺑ ﻪﻠﺠﻣ
ﻲﻤﻠﻋ
-
ﻲﺸﻫوﮋﭘ
و
ISC
ﻪﻌﻟﺎﻄﻣ ﻲﻠﻜﺷﺪﻨﭼ نژ
IGFBP3
ﻪﺷﻻ تﺎﻔﺻ ﺎﺑ نآ طﺎﺒﺗرا و بﺮﭼ يﺎﻫﺪﻴﺳا ﺐﻴﻛﺮﺗ و ﻲﺧﺮﺑ و
ﻪﺠﻨﺳاﺮﻓنﻮﺧ يﺎﻫ رد ﺪﻨﻔﺳﻮﮔ داﮋﻧ لزﺑﺎ شور
PCR-SSCP
دﺎﺷ ﻲﻧﺎﺧ ﻲﺿﺎﻗ ﻲﻠﻋ
∗
∗∗
∗1
ﻚﺑﺎﺑﺮﻬﺷ يداﺮﻣ ﻦﻴﺴﺣ ،
2
ﻲﻗدﺎﺻ ﻲﻔﻄﺼﻣ ،
2
ﻲﺟﺮﻓ ﺎﺿر ،
3
يدﺎﺑآﺪﻤﺤﻣ ﺎﺿر ﺪﻤﺤﻣ ،
4
1
،هوﺎﺳ ﺪﺣاو ،ﻲﻣﻼﺳا دازآ هﺎﮕﺸﻧادﻠﻋ هوﺮﮔ ،ﻲﻣاد مﻮناﺮﻳا ،هوﺎﺳ
2
رﺎﻳدﺎﺘﺳاناﺮﻳا ،جﺮﻛ ،ﻲﻣاد مﻮﻠﻋ هوﺮﮔ ،ناﺮﻬﺗ هﺎﮕﺸﻧاد ﻲﻌﻴﺒﻃ ﻊﺑﺎﻨﻣ و يزروﺎﺸﻛ ﺲﻳدﺮﭘ ،
3
ناﺮﻳا ،جﺮﻛ ،ﻲﻣاد مﻮﻠﻋ هوﺮﮔ ،ناﺮﻬﺗ هﺎﮕﺸﻧاد ﻲﻌﻴﺒﻃ ﻊﺑﺎﻨﻣ و يزروﺎﺸﻛ ﺲﻳدﺮﭘ ،ﺪﺷرا ﻲﺳﺎﻨﺷرﺎﻛ يﻮﺠﺸﻧاد
4
نﺎﻣﺮﻛ ﺮﻨﻫﺎﺑ ﺪﻴﻬﺷ هﺎﮕﺸﻧاد رﺎﻴﺸﻧاد ،ﻲﻣاد مﻮﻠﻋ هوﺮﮔ ،نﺎﻣﺮﻛ، ناﺮﻳا
ﺖﻓﺎﻳرد ﺦﻳرﺎﺗ :19/07/1391شﺮﻳﺬﭘ ﺦﻳرﺎﺗ ، :17/01/1392
هﺪﻴﻜﭼ
نﻮﻣرﻮﻫ
IGF1
ﺮﭘ زا ﻲﻜﻳ ﻪﺑ ﻚﻳژﻮﻟﻮﻴﺑ تﺎﻌﻳﺎﻣ ردﻦﻴﺌﺗوﻲﻣ ﻞﺼﺘﻣ هﺪﻧﻮﺷ ﺪﻧﺎﺑ يﺎﻫدﻮﺷ ﻦﻳﺮﺗ هﺪﻤﻋ ﻪﻛ
ﭗﻴﺗ ﺎﻬﻧآ3 )
3
IGFBP
(ﻲﻣﺪﺷﺎﺑ . ﻞﻣﺎﺣ ناﻮﻨﻋ ﻪﺑ ﻦﻴﺌﺗوﺮﭘ ﻦﻳا
IGF1
دﺮﻛ ﻞﻤﻋﻪﻤﻴﻧ و هﻲﻧﻻﻮﻃ ار نآ ﺮﻤﻋ ﺮﺗ
ﻲﻣو ﺪﻨﻛ ﻲﻜﻳژﻮﻟﻮﻴﺑ ﺖﻴﻟﺎﻌﻓ ﺶﻫﺎﻛ ﺎﻳ ﺶﻳاﺰﻓا ﺐﺒﺳ ﻲﻟﻮﻠﺳ ﺢﻄﺳ رد
IGF
ﻲﻣدﻮﺷ. فﺪﻫ ﺎﺑ ﺮﺿﺎﺣ ﻖﻴﻘﺤﺗ
تﺎﻔﺻ ﺎﺑ نژ ﻦﻳا ﻲﻠﻜﺷﺪﻨﭼ طﺎﺒﺗرا ﻪﻌﻟﺎﻄﻣبﺮﭼ ﺪﻴﺳا تﺎﺒﻴﻛﺮﺗ و ﻪﺷﻻ رد ﺪﻨﻔﺳﻮﮔ داﮋﻧﻲﺑﻪﺒﻧد ﺑ لزﺎ شور
PCR-SSCP
ﺪﺷ مﺎﺠﻧا .ﺶﻫوﮋﭘ ﻦﻳا رد 130 سار ﺪﻨﻔﺳﻮﮔزدرﻮﻣ ﻲﻓدﺎﺼﺗ رﻮﻃ ﻪﺑ ل يﺮﻴﮔ نﻮﺧ وﺒﺛ ﺖ
ﻪﺷﻻ تﺎﻔﺻﺘﻓﺮﮔ راﺮﻗﺪﻨ .بﺮﭼ يﺎﻫﺪﻴﺳا ﻪﻌﻟﺎﻄﻣ ياﺮﺑ، ﺎﺑ يﺮﻴﮔ نﻮﺧﺖﻜﺟﻮﻧو يﺎﻫ
EDTA
راد ﺪﺷ مﺎﺠﻧا
ﻪﻠﺻﺎﻓﻼﺑ و ﺎﻤﺳﻼﭘ يزﺎﺳاﺪﺟ ياﺮﺑ ﺎﻫ ﻪﻧﻮﻤﻧﺪﺷ ژﻮﻴﻔﻳﺮﺘﻧﺎﺳﺪﻧ . جاﺮﺨﺘﺳا
DNA
زا ﺖﻴﻛ ﻪﻠﻴﺳﻮﺑ ﻪﭽﻴﻫﺎﻣ ﺖﻓﺎﺑ
ﺖﻛﺮﺷ
BIOTECH
ﺖﻓﺮﮔ ترﻮﺻ. ﺲﭙﺳ
PCR
ياﺮﺑ ﻴﺜﻜﺗ ﻪﻌﻄﻗ ﺮ654 ﺖﻔﺟيزﺎﺑ نژ
IGFBP3
مﺎﺠﻧا
ﺖﻓﺮﮔ . ﻦﻴﻴﻌﺗ ياﺮﺑﻲﻠﻜﺷﺪﻨﭼ ﻪﻧﻮﻤﻧﺎﻫ، تﻻﻮﺼﺤﻣ زرﻮﻓوﺮﺘﻜﻟا
PCR
ﻚﺗ زا ﺲﭘتﺎﻌﻄﻗ نﺪﺷ ﻪﺘﺷر ) شور
SSCP
( ﺪﺷ مﺎﺠﻧا هﺮﻘﻧ تاﺮﺘﻴﻧ شور ﻪﺑ لژ يﺰﻴﻣآ ﮓﻧر و ﺪﻴﻣآ ﻞﻳﺮﻛا لژ يور. ﺞﻳﺎﺘﻧ دﻮﺟو ﺮﮕﻧﺎﻴﺑ يﺎﻫﻮﮕﻟا
توﺎﻔﺘﻣ دﻮﺑﻲﻣ ﻪﻛ زا ﻲﺷﺎﻧ ﺪﻧاﻮﺗ دﻮﺟوﻲﻠﻜﺷﺪﻨﭼ ردﻦﻳا ﺑ هﺎﮕﻳﺎﺟﺷﺎﺪ .اﺮﻓ ﻲﭙﻴﺗﻮﻧژ يﺎﻫﻮﮕﻟا ﻲﻧاو1 ،2 ،3 ،4
و5 نژ
IGFBP3
رد ﺖﻴﻌﻤﺟ ﻦﻳا ﺐﻴﺗﺮﺗ ﻪﺑ67/66 ،33/3 ،67/6 ،33/13 و10 ﺪﺻرددﻮﺑ
.
نزو تﺎﻔﺻ
مﺮﮔ ﻪﺷﻻ )
P<0.05
(ﺖﺸﭘ ﻲﺑﺮﭼ ﺖﻣﺎﺨﺿ ، )
P<0.01
(ﺖﺷﻮﮔ مﺎﺧ ﻦﻴﺌﺗوﺮﭘ و)
P<0.01
( راد ﻲﻨﻌﻣ طﺎﺒﺗرا
ﺎﺑﻮﮕﻟاﺪﻧداد نﺎﺸﻧ ﻒﻠﺘﺨﻣ يﺎﻫ .بﺮﭼ ﺪﻴﺳا تﺎﺒﻴﻛﺮﺗ درﻮﻣرد راد ﻲﻨﻌﻣ طﺎﺒﺗرا ﺎﻬﻨﺗ ﻢﻫﺪﻴﺳا ﻦﻴﺑ بﺮﭼ
ﻚﻴﺋﻮﻧﺎﻛدﺎﺘﭙﻫ )
C17:0
( ﺎﺑﻮﮕﻟا نژ ﻒﻠﺘﺨﻣ يﺎﻫ
IGFBP3
ﺪﺷ هﺪﻫﺎﺸﻣ)
P<0.01
( . و تﺎﻔﺻ ﺮﻳﺎﺳ ﻦﻴﺑ
ﺎﺑ يراد ﻲﻨﻌﻣ طﺎﺒﺗرا ﭻﻴﻫ بﺮﭼ يﺎﻫﺪﻴﺳاﻮﮕﻟا يﺎﻫ
IGFBP3
ﻫﺎﺸﻣ ﻖﻴﻘﺤﺗ ﻦﻳا ردﺪﺸﻧ هﺪ.
يﺪﻴﻠﻛ تﺎﻤﻠﻛ :
تﺎﻔﺻ ﻪﺷﻻ ،
IGFBP3
،ﻲﻠﻜﺷ ﺪﻨﭼ،
PCR-SSCP
، ﻫﺪﻴﺳابﺮﭼ يﺎلز ،.
∗ ﺴﻣ هﺪﻨﺴﻳﻮﻧﺌلﻮ :دﺎﺷ ﻲﻧﺎﺧ ﻲﺿﺎﻗ ﻲﻠﻋ ﻦﻔﻠﺗ: 09121466570 A.ghazikhani@iau-saveh.ac.ir Email:

دﺎﺷ ﻲﻧﺎﺧ ﻲﺿﺎﻗ ،نارﺎﻜﻤﻫ و1392
٩٨
ﻪﻣﺪﻘﻣ
هزوﺮﻣا رد ﺮﻣا ﺪﻨﻔﺳﻮﮔ شروﺮﭘ، ﻠﻣاﻮﻋﻲ ﺮﻴﻈﻧ
ﻢﺘﺴﻴﺳ ،ﻲﻤﻴﻠﻗا ﻂﻳاﺮﺷﺎﻫ ﻂﻳاﺮﺷ ،زﺎﺑ ﻲﺷروﺮﭘ ي
و ﺮﻴﻘﻓ ﻲﻄﻴﺤﻣﺖﻳدوﺪﺤﻣيدﺎﺼﺘﻗا يﺎﻫ ﻚﻳ زا
فﺮﻃ و ﻦﻴﻨﭽﻤﻫ زا مدﺮﻣ لﺎﺒﻘﺘﺳا مﺪﻋ ﻲﺑﺮﭼ ) ﻪﺑ
يرﺎﻤﻴﺑ ﺎﺑ نآ طﺎﺒﺗرا ﻞﻴﻟدﻲﻗوﺮﻋ و ﻲﺒﻠﻗ يﺎﻫ( زا
ﺮﮕﻳد فﺮﻃ ﺚﻋﺎﺑﺰﻓا زور ﺶﻫﺎﻛ نو يﺪﻨﺴﭘ رازﺎﺑ
وﻲﺑﺮﭼ ﺪﻴﻟﻮﺗ يدﺎﺼﺘﻗا ﻪﻓﺮﺻ) ﻦﻴﺑ ﻲﺑﺮﭼ و ﻪﺒﻧد
ﻲﺘﻓﺎﺑ (ﺖﺳا هﺪﺷ. ﻦﻳاﺮﺑﺎﻨﺑﻲﻣ ﺮﻈﻧ ﻪﺑ ﻪﻛ ﺪﺳر
ﺶﻫﺎﻛﻲﺑﺮﭼ ﻪﺑ ﺖﺒﺴﻧ ﻦﻴﺌﺗوﺮﭘ ﻪﺷﻻ ﺮﻴﻴﻐﺗ و
ﺶﻫﺎﻛ ﺖﻤﺳ ﻪﺑ ﺖﺷﻮﮔ بﺮﭼ يﺎﻫﺪﻴﺳا ﺐﻴﻛﺮﺗ
ﻲﺑﺮﭼعﺎﺒﺷا يﺎﻫ )ﺖﺨﺳ (ﺪﻴﺳا ﺶﻳاﺰﻓا و يﺎﻫ
بﺮﭼ ﺎﮕﻣا ﺮﻴﻈﻧ بﻮﻠﻄﻣ 3 )
C18:3
(ﺎﮕﻣا و 6
)
C18:2
( ،ياﺮﺑ ﻪﺷﻻ ﺖﻴﻔﻴﻛ دﻮﺒﻬﺑ و مزﻻ يﺮﻣا
ﻲﻣ بﻮﺴﺤﻣ يدﺎﺼﺘﻗادﻮﺷ .راﺪﻘﻣ ﺶﻫﺎﻛ ﻲﺑﺮﭼ
ود زارشو يروآ دﻮﺳ ﺮﺑﺖﺳا راﺬﮔﺮﺛا: ﻒﻟا -
ﻪﺷﻻ ﺖﻴﻔﻴﻛ دﻮﺒﻬﺑ و ﺪﺷر ﺖﻋﺮﺳ ﺶﻳاﺰﻓا ﻞﻴﻟد ﻪﺑ
يﺪﻨﺴﭘ رازﺎﺑ ﺶﻳاﺰﻓا ﻲﻣ ﺪﻣآرد ﺶﻳاﺰﻓا ﻪﺑ ﺮﺠﻨﻣ ،
ددﺮﮔ. ب- ا ﻪﺑ اﺬﻏ ﻪﻨﻳﺰﻫ ﺶﻫﺎﻛ مﺮﮔﻮﻠﻴﻛ ﺮﻫ ياز
هﺪﻧز نزو ﺶﻳاﺰﻓا ﺚﻋﺎﺑ ﺰﻴﻧ ﻪﺷﻻ ﺎﻳ دﻮﺳ ناﺰﻴﻣ
ﻞﺻﺎﺣﻲﻣدﻮﺷ )
Martin and Baxter, 1992
(.
ﺖﻓﺮﮔ راﺮﻗ ﻲﺳرﺮﺑ درﻮﻣ لز داﮋﻧ ﻖﻴﻘﺤﺗ ﻦﻳا رد .
داﮋﻧ لز ﻲﺘﻤﺴﻗ و رﺰﺧ يﺎﻳرد ﻲﻠﺣﺎﺳ ﻖﻃﺎﻨﻣ رد
ﻲﻣ هداد شروﺮﭘ نﺎﮔﺮﮔ ﺖﺷد زادﻮﺷ . ﺖﻴﺻﻮﺼﺧ
لز ﺪﻨﻔﺳﻮﮔ دﺮﻓ ﻪﺑ ﺮﺼﺤﻨﻣ يﺎﺟ ﻪﺑ مد دﻮﺟو
ﺖﺳا ﻪﺒﻧد. رﻮﺤﻣﺗﺎﻣﻮﺳ ﻚﻴﭘوﺮﺗﻮ)
IGFBP-IGF-
GH
( ﻪﻛ هدﻮﺑ نﺪﺑ ﻲﻧﻮﻣرﻮﻫ ﻢﺘﺴﻴﺳ ﻦﻳﺮﺘﻤﻬﻣ
و ﻢﺴﻴﻟﻮﺑﺎﺘﻣ ﻢﻴﻈﻨﺗ يﺎﻫﺪﻨﻳاﺮﻓ ﻲﻜﻳژﻮﻟﻮﻳﺰﻴﻓ
ﺪﺷر ﺎﺑ ﻂﺒﺗﺮﻣﻲﻣ لﺮﺘﻨﻛ ارﺪﻨﻛ . ﻞﻣﺎﺷ ﻢﺘﺴﻴﺳ ﻦﻳا
،ﺪﺷر نﻮﻣرﻮﻫ
IGF-1
) ﭗﻴﺗ1و2(يﺎﻫ هﺪﻧﺮﻴﮔ ،
ﻪﺑ طﻮﺑﺮﻣ
IGF
) ﭗﻴﺗ1 و2( و6 ﺪﻧﺎﺑ ﻦﻴﺌﺗوﺮﭘ
هﺪﻧﻮﺷ
(IGFBP1-6)
ﻲﻣﺪﺷﺎﺑ )3 و14( . نﻮﻣرﻮﻫ
يﺎﻫ
IGF-1
و
IGF-2
يا هﺮﻴﺠﻧز ﻚﺗ يﺎﻫ ﺪﻴﺘﭙﻴﭘ ،
ﻲﻟﻮﻜﻟﻮﻣ نزو ﺎﺑ7500 ﺪﻨﺷﺎﺑ ﻲﻣ نﻮﺘﻟاد
)
Morimoto et al, 2005
(.
IGF-1
زا70 ﺪﻴﺳا
و ﻪﻨﻴﻣآ
IGF-2
زا67 هﺪﺷ ﻞﻴﻜﺸﺗ ﻪﻨﻴﻣآ ﺪﻴﺳا
ﺎﻬﻧآ يﺎﻫ ﻪﻨﻴﻣآﺪﻴﺳا ﻲﻟاﻮﺗ و ﺖﺳا62 ﻢﻫ ﺎﺑ ﺪﺻرد
ﻲﻣ ﻪﺑﺎﺸﻣﺪﺷﺎﺑ )
Moradi Shahrebabak et al,
2009
( . يﺎﻫ نﻮﻣرﻮﻫ ،ﻦﻴﻟﻮﺴﻧا فﻼﺧﺮﺑ
IGF
رد
زا ﻲﻜﻳ ﻪﺑ ﻲﻜﻳژﻮﻟﻮﻴﺑ تﺎﻌﻳﺎﻣ 6 ﺪﻧﺎﺑ ﻦﻴﺌﺗوﺮﭘ
هﺪﻧﻮﺷ
(IGFBP)
ﻦﻳﺮﺗ هﺪﻤﻋ ﻪﻛ ﺪﻧﻮﺷ ﻲﻣ ﻞﺻو
ﭗﻴﺗ ﻦﻴﺌﺗوﺮﭘ ﺎﻬﻧآ3 ﺪﺷﺎﺑ ﻲﻣ. ﺎﻫ ﻦﻴﺌﺗوﺮﭘ ﻦﻳا
ﻲﻜﻳژﻮﻟﻮﻴﺑ ﺖﻴﻟﺎﻌﻓ ﻢﻴﻈﻨﺗ رد ﻲﺗوﺎﻔﺘﻣ يﺎﻫ ﺶﻘﻧ
IGF
ﺪﻨﻨﻛ ﻲﻣ ءﺎﻔﻳا )
Chudhary et al, 2007
( .
ﺗوﺮﭘ ﭗﻴﺗ هﺪﻧﻮﺷ ﺪﻧﺎﺑ ﻦﻴﺌ3 ﻞﻣﺎﺣ ناﻮﻨﻋ ﻪﺑ
IGF
،ﺪﻨﻛ ﻲﻣ ﺮﺗ ﻲﻧﻻﻮﻃ ار نآ ﺮﻤﻋ ﻪﻤﻴﻧ و هدﺮﻛ ﻞﻤﻋ
ﻲﻟﻮﻠﺳ ﺢﻄﺳ رد
IGFBP
ﺎﻫ ﻲﻣ لﺎﻌﻓ ﺐﺒﺳ ﺪﻨﻧاﻮﺗ
نﻮﻣرﻮﻫ ﻲﻜﻳژﻮﻟﻮﻴﺑ ﺖﻴﻟﺎﻌﻓ نﺪﺷ لﺎﻌﻓ ﺮﻴﻏ ﺎﻳ
IGF
ﺪﻧﻮﺷ)
Hastie et al, 2004
.( ﻦﻴﺌﺗوﺮﭘ نژ
ﭗﻴﺗ ﻦﻴﻟﻮﺴﻧا ﻪﺒﺷ ﺪﺷر رﻮﺘﻛﺎﻓ ﻪﺑ هﺪﻧﻮﺷ ﺪﻧﺎﺑ3
ﺧﺎﺳ نژ نژ زا يدﺪﻌﺘﻣ تاﺮﺛا لﻮﺌﺴﻣ و يرﺎﺘ
IGF-1
ﺖﺳا )
Cheong et al, 2008
.( نژ
IGFBP3
و ﺺﺨﺸﻣ وﺎﮔ رد
mRNA
نآ نﻮﻠﻛ
ﻞﻣﺎﺷ ﻪﻛ ﺖﺳا هﺪﺷ يزﺎﺳ1650 ﺖﻔﺟ- زﺎﺑ

يزروﺎﺸﻛ يژﻮﻟﻮﻨﻜﺗﻮﻴﺑ ﻪﻠﺠﻣ) هرود5 هرﺎﻤﺷ ،4 ،نﺎﺘﺴﻣز 1392(
٩٩
ﺖﺳا . ياراد نژ ﻞﻛ8500 ﺖﻔﺟ ﻞﻣﺎﺷ و زﺎﺑ5
ﻲﻣ نوﺰﮔاﺪﺷﺎﺑ )
Chudhary et al, 2007
.( نژ
ﻮﺴﻧا ﻪﺒﺷ ﺪﺷر رﻮﺘﻛﺎﻓ ﻪﺑ هﺪﻧﻮﺷ ﺪﻧﺎﺑ ﻦﻴﺌﺗوﺮﭘ ﻦﻴﻟ
ﭗﻴﺗ3 مزﻮﻣوﺮﻛ يور نﺎﺴﻧا رد7 هﺪﺷ ﺺﺨﺸﻣ
)ﺖﺳا هﺪﺸﻧ ﻲﺑﺎﻳ نﺎﻜﻣ زﻮﻨﻫ ﺪﻨﻔﺳﻮﮔ رد .(
ﻲﺑﺎﻳ ﻲﻟاﻮﺗ و ﻲﻠﻜﺷﺪﻨﭼ تﺎﻌﻟﺎﻄﻣ يﺎﻫﺪﻴﺗﻮﺌﻠﻛﻮﻧ
هﺪﺷ شراﺰﮔ ﺶﻴﻣوﺎﮔ و وﺎﮔ رد نژ ﻦﻳا، ﺎﻣا
يﺮﻛذ نﺎﻳﺎﺷ ﻲﺳرﺮﺑ نﻮﻨﻛ ﺎﺗ ﺪﻨﻔﺳﻮﮔ درﻮﻣرد
ﺖﺳا ﻪﺘﻓﺮﮕﻧ ترﻮﺻ . تﺎﻌﻄﻗ ﻲﻟاﻮﺗ ﻪﭼﺮﮔ
mRNA
ﺮﺑ ﺪﻨﻔﺳﻮﮔ رد نژ ﻦﻳا ﺖﻳﺎﺳ رد و ﻲﺳر
GEN BANK
ﺖﺳا هﺪﻳدﺮﮔ ﺖﺒﺛ. ا نژ ﻦﻴﺑ طﺎﺒﺗر
IGFBP3
ﻪﺷﻻ تﺎﻔﺻ ﺎﺑ وﺎﮔ رد شراﺰﮔ هﺪﺷ
ﺖﺳا. )
Sun, 2002
.( ،ﺮﺿﺎﺣ ﻖﻴﻘﺤﺗ زا فﺪﻫ
ﻲﻠﻜﺷﺪﻨﭼ ﻲﻳﺎﺳﺎﻨﺷرد دﻮﺟﻮﻣ يﺎﻫ نوﺰﮔا3 نژ
ﭗﻴﺗ هﺪﻧﻮﺷ ﺪﻧﺎﺑ ﻦﻴﺌﺗوﺮﭘ3 )
IGFBP3
( رﻮﺘﻛﺎﻓ ﻪﺑ
ﻦﻴﻟﻮﺴﻧا ﻪﺒﺷ ﺪﺷر وﻲﻧاواﺮﻓ ناﺰﻴﻣ دروآﺮﺑ
ﺎﻫﻮﮕﻟا ﻲﺳرﺮﺑ و هﺎﮕﻳﺎﺟ ﻦﻳا ﻒﻠﺘﺨﻣ ﻲﭙﻴﺗﻮﻧژ ي
ﻦﻳا طﺎﺒﺗرا ﺪﻨﭼﻲﻠﻜﺷ ﺎﻫ ﺪﺷر تﺎﻔﺻ ﺎﺑ ﺐﻴﻛﺮﺗ و
بﺮﭼ يﺎﻫﺪﻴﺳا ﻚﻴﻨﻜﺗ زا هدﺎﻔﺘﺳا ﺎﺑ لز داﮋﻧ رد
PCR-SSCP
دﻮﺑ
.
يﺎﺘﺳار رد ناﻮﺘﺑ ﺖﻳﺎﻬﻧ رد ﻪﻛ
زا بﺮﭼ يﺎﻫﺪﻴﺳا تﺎﺒﻴﻛﺮﺗ و ﻪﺷﻻ تﺎﻔﺻ دﻮﺒﻬﺑ
ماد ﻚﻴﻣﻮﻧژ بﺎﺨﺘﻧا و ﻲﻳﺎﺳﺎﻨﺷ ظﺎﺤﻟ زا ﺮﺗﺮﺑ يﺎﻫ
نژ ﻦﻳاﺖﻓﺮﮔ ﻚﻤﻛ.
شور و داﻮﻣﺎﻫ
هزاﺪﻧا يﺎﻫدرﻮﻛر زا هدﺎﻔﺘﺳا ﺎﺑ ﻪﻌﻟﺎﻄﻣ ﻦﻳا
يور هﺪﺷ يﺮﻴﮔ120 ياﺮﺑ ﻪﻛ لز ﺪﻨﻔﺳﻮﮔ سأر
ﺮﺧاوا ﺎﺗ هﺎﻣ ﺮﻴﺗ ﻂﺳوا زا ﻲﺘﻌﻨﺻ هﺎﮔرﺎﺘﺸﻛ ﻪﺑ ﺢﺑذ
لﺎﺳ هﺎﻣ رﻮﻳﺮﻬﺷ1388 مﺎﺠﻧا ،ﺪﻧدﻮﺑ هﺪﺷ هدروآ
ﺖﻓﺮﮔ . ترﻮﺻ ﻪﺑ ﺶﻳﺎﻣزآ درﻮﻣ ناﺪﻨﻔﺳﻮﮔ
را ناﺪﻨﻔﺳﻮﮔ ﻦﻴﺑ زا ﻲﻓدﺎﺼﺗ شوﺮﻓ ﺖﻬﺟ هﺪﺷ ﻪﺋا
ﺪﻧﺪﻳدﺮﮔ بﺎﺨﺘﻧا . لﻼﺧ رد ﻪﻛ ترﻮﺻ ﻦﻳﺪﺑ
ﻪﺘﻔﻫ ﺮﻫ رد يرادﺮﺑ هداد تﺪﻣ5 ﺎﺗ6 ﻪﺑ زور
ﻲﻣ ﻪﻌﺟاﺮﻣ هﺎﮔرﺎﺘﺸﻛ ﻪﻧازور ﻂﺳﻮﺘﻣ رﻮﻃ ﻪﺑ و ﺪﺷ
7 ﺎﺗ8 درﻮﻣ ﻲﻓدﺎﺼﺗ رﻮﻃ ﻪﺑ ﺪﻨﻔﺳﻮﮔ سأر
ﻲﻣ راﺮﻗ يرادﺮﺑ درﻮﻛرﺖﻓﺮﮔ . درﻮﻣ ناﺪﻨﻔﺳﻮﮔ
ﻤﻴﻠﻗا ﻂﻳاﺮﺷ ﺖﺤﺗ و ﻒﻠﺘﺨﻣ ﻦﻴﻨﺳ ﻦﻴﺑ ﻪﻌﻟﺎﻄﻣ ،ﻲ
ﺪﻨﺘﺷاد راﺮﻗ ﻲﺗوﺎﻔﺘﻣ ﻲﺘﻳﺮﻳﺪﻣ و ﻲﺷروﺮﭘ . ﻪﺑ
زا ﺲﭘ ﻪﻠﺻﺎﻓﻼﺑ ﺎﻬﻧآ زا ﻲﺧﺮﺑ ﻪﻛ يرﻮﻃ
هرود ﺮﮕﻳد ﻲﺧﺮﺑ و يﺮﻴﮔﺮﻴﺷ توﺎﻔﺘﻣ يﺎﻫ
هﺮﻴﺟ زا هدﺎﻔﺘﺳا ﺎﺑ ار يراروﺮﭘ ﺎﻳ و ﻒﻠﺘﺨﻣ يﺎﻫ
راﺰﺘﺸﻛ و ﻲﻋارز نﺎﻫﺎﻴﮔ ﺮﭼ ﺲﭘ يور ﺮﺑ اﺮﭼ
ﺪﻧدﻮﺑ هﺪﺷ ﻪﺿﺮﻋ رﺎﺘﺸﻛ ياﺮﺑ و هدﺮﻛ ﻲﻃ ﻪﺠﻧﻮﻳ .
اراد ﻪﻠﮔ ﺐﻠﻏا ﻞﺒﻗ ﺐﺷ ﻚﻳ ار دﻮﺧ ناﺪﻨﻔﺳﻮﮔ ،ن
ﻲﻣ ﻞﻤﺣ هﺎﮔرﺎﺘﺸﻛ رﺎﻈﺘﻧا ﻦﻟﺎﺳ ﻪﺑ رﺎﺘﺸﻛ زاﺪﻧدﺮﻛ .
ﺖﻬﺟ هﺪﺷ ﻪﺘﻓﺮﮔ ﺮﻈﻧ رد ناﺪﻨﻔﺳﻮﮔ رﺎﺘﺸﻛ زا ﻞﺒﻗ
ندﺮﻛ ناﺰﻳوآ ﺎﺑ ،ﺮﻈﻧ درﻮﻣ يﺎﻫدرﻮﻛر يﺮﻴﮔ هزاﺪﻧا
و يراﺬﮔ هرﺎﻤﺷ ،ﺎﻬﻧآ ندﺮﮔ ﻪﺑ ﻲﺘﻗﻮﻣ كﻼﭘ
ﻲﻣ ﻲﻳﺎﺳﺎﻨﺷﺪﻧﺪﺷ . ،ﺲﻨﺟ ،يراﺬﮔ هرﺎﻤﺷ زا ﺲﭘ
ﻦﺳ)لاﻮﺳ ﺎﺑ ناﺪﻧد ﻲﺑﺎﻳزرا و راﺪﻣاد زاﺎﻫ ( نزو و
ﻲﻣ ﻦﻴﻴﻌﺗ ﺎﻬﻧآﺪﺷ . ﻚﻳ رد ﺪﻨﻔﺳﻮﮔ ﻪﻛ ﻲﻟﺎﺣ رد
ﻲﻣ رﺎﻬﻣ و ﺖﺑﺎﺛ هﺎﮕﻳﺎﺟ مﺎﺠﻧا ياﺮﺑ يﺮﻴﮕﻧﻮﺧ ،ﺪﺷ
جاﺮﺨﺘﺳا
DNA
هزاﺪﻧا ﺖﻬﺟ ﺎﻤﺳﻼﭘ ﻦﺘﻓﺮﮔ و
ﻲﻣ ترﻮﺻ ﻲﻧﻮﺧ يﺎﻫﺮﺘﻣارﺎﭘ يﺮﻴﮔﺖﻓﺮﮔ . ﺲﭘ
يﺮﻴﮔ هزاﺪﻧا مﺎﻤﺗا زانﻮﺧ و ﺎﻫ ناﺪﻨﻔﺳﻮﮔ ،يﺮﻴﮔ

دﺎﺷ ﻲﻧﺎﺧ ﻲﺿﺎﻗ ،نارﺎﻜﻤﻫ و1392
١٠٠
ﻮﺳﺮﻣ شور ﻪﺑ ﺪﻧﺪﺷ ﺢﺑذ ،ﻲﺘﻌﻨﺻ هﺎﮔرﺎﺘﺸﻛ رد م
و ءﺎﻌﻣا ﻪﻴﻠﺨﺗ ،ﻲﻨﻛ ﺖﺳﻮﭘ ،يﺮﻴﮕﻧﻮﺧ زا ﺪﻌﺑ و
ﺮﻈﻧ زا ﻪﺷﻻ ﻲﺑﺎﻳزرا ،ﻲﻨﻄﺑ هﺮﻔﺣ زا ءﺎﺸﺣا
ﻲﻣ ترﻮﺻ ﻚﺷﺰﭙﻣاد ﻂﺳﻮﺗ ﻲﺘﺷاﺪﻬﺑﺖﻓﺮﮔ .
ﻦﻴﺑ ﻪﻴﺣﺎﻧ رد ﺖﺸﭘ ﻲﺑﺮﭼ ﺖﻣﺎﺨﺿ يﺮﻴﮔ هزاﺪﻧا
مﺎﺠﻧا ﺲﻴﻟﻮﻛ زا هدﺎﻔﺘﺳا ﺎﺑ هدﺰﻴﺳ و هدزاود هﺪﻧد
ﻲﻣﺖﻓﺮﮔ .ﻻ ﺮﺧآ ﻪﻠﺣﺮﻣ رد ﺪﻌﺑ و ،ﻦﻳزﻮﺗ مﺮﮔ ﻪﺷ
ﻪﺒﻧد نوﺪﺑ ﻪﺷﻻ هرﺎﺑود ،نﺪﺑ زا ﻪﺒﻧد ندﺮﻛ اﺪﺟ زا
ﻲﻣ ﺖﺒﺛ و ﻦﻳزﻮﺗﺪﻳدﺮﮔ . ﺮﻫ ﻚﻴﻜﻔﺗ ﻪﺑ ﻪﺒﻧد نزو
نوﺪﺑ مﺮﮔ ﻪﺷﻻ نزو ندﺮﻛ ﻢﻛ زا ،ﺪﻨﻔﺳﻮﮔ سأر
ﺪﻳدﺮﮔ ﺖﺒﺛ و ﻪﺒﺳﺎﺤﻣ مﺮﮔ ﻪﺷﻻ ﻞﻛ نزو زا ﻪﺒﻧد.
- ﺖﻜﺟﻮﻧو هﺎﮔرﺎﺘﺸﻛ رد نﻮﺧ يوﺎﺣ يﺎﻫ
ژﻮﻴﻔﻳﺮﺘﻧﺎﺳ)10 ﺮﺳ ﺎﺑ ﻪﻘﻴﻗد ﺖﻋ
g
3000 ( ﺪﻧﺪﺷ
و مﺮﺳ دﻮﺷ اﺪﺟ ﻞﻣﺎﻛ رﻮﻄﺑ مﺮﺳ و ﺎﻤﺳﻼﭘ ﺎﺗ
پﻮﻴﺗوﺮﻜﻴﻣ ﻞﺧاد هﺪﺷ اﺪﺟ يﺎﻤﺳﻼﭘ5/1 ﻲﻠﻴﻣ
ﻲﻣ ﻪﺘﺨﻳر يﺮﺘﻴﻟ رد يراﺬﮔ هرﺎﻤﺷ زا ﺪﻌﺑ و ﺪﺷ
يﺎﻣد رد لﺎﭽﺨﻳ20- يراﺪﻬﮕﻧ داﺮﮕﻴﺘﻧﺎﺳ ﻪﺟرد
ﺪﺷ.
- جاﺮﺨﺘﺳا ياﺮﺑ
DNA
دﻮﺟو ﻲﻔﻠﺘﺨﻣ يﺎﻬﺷور
دراد . جاﺮﺨﺘﺳا ﺶﻫوﮋﭘ ﻦﻳا رد
DNA
ﺎﺑ زا ﺖﻓ
ﻪﻠﻴﺳﻮﺑ ﻪﭽﻴﻫﺎﻣ ﺎﺑ ﻚﺗﻮﻳﺎﺑ ﺖﻛﺮﺷ ﺖﻴﻛ ي
ﺪﺷ مﺎﺠﻧا هﺪﺷ ﻪﻴﺻﻮﺗ ﻲﺻﺎﺼﺘﺧا ﻞﻛﻮﺗوﺮﭘ.
جاﺮﺨﺘﺳا ياﺮﺑ شور ﻦﻳا ﻞﻜﺗوﺮﭘ
DNA
زا2-
5/1 ﻲﻠﻴﻣﻲﻣ ﺮﻳز حﺮﺷ ﻪﺑ ﻞﻣﺎﻛ نﻮﺧ ﺮﺘﻴﻟﺪﺷﺎﺑ:
1- ناﺰﻴﻣ ﻪﺑ هﺪﻨﻨﻛ اﺪﺟ ﺮﻓﺎﺑ ندﺮﻛ ﻪﻓﺎﺿا10 ﻲﻠﻴﻣ
ﺮﺘﻴﻟ) زرﺎﻛﺎﺳ32/0 ،رﻻﻮﻣ
MgCl
2
5
،رﻻﻮﻣ ﻲﻠﻴﻣ
Tris-HCl
10 ،رﻻﻮﻣ ﻲﻠﻴﻣ
100X
Triton
1
،ﺪﺻرد6/7=
pH
.( ندﺮﺑ ﻦﻴﺑ زا ﺖﻬﺟ ﺮﻓﺎﺑ ﻦﻳا زا
لﻮﺳ نورد زا ﻪﺘﺴﻫ ندﺮﻛ جرﺎﺧ و ﻲﻟﻮﻠﺳ ءﺎﺸﻏ
ﻲﻣ هدﺎﻔﺘﺳادﻮﺷ .
2- ﻪﻧﻮﻤﻧ ﺲﻜﺗرو ﻪﺑ ﺦﻳ يور ﺮﺑ نداد راﺮﻗ و ﺎﻫ
تﺪﻣ10 رد ژﻮﻴﻔﻳﺮﺘﻧﺎﺳ و ﻪﻘﻴﻗد3000 رد رود
تﺪﻣ ﻪﺑ ﻪﻘﻴﻗد10 ﻪﻘﻴﻗد
3- ﻲﻳور ﻊﻳﺎﻣ ندﺮﻛ جرﺎﺧ بﻮﺳر يراﺪﻬﮕﻧ و
4- ﺪﻴﻔﺳ بﻮﺳر ﻞﻴﻜﺸﺗ ﺎﺗ قﻮﻓ ﻞﺣاﺮﻣ راﺮﻜﺗ
5- هﺪﻨﻨﻛ ﺰﻴﻟ ﺮﻓﺎﺑ ندﺮﻛ ﻪﻓﺎﺿا)
Tris-HCl
10
رﻻﻮﻣ ﻲﻠﻴﻣ
pH=8.2
،
NaCl
400 mM
،
EDTA
2Mm
pH=8
( ناﺰﻴﻣ ﻪﺑ300 وﺮﻜﻴﻣ ﺲﭙﺳ و ﺮﺘﻴﻟ
ﻲﻣ ﺲﻜﺗرو رﺪﻘﻧآدﻮﺷ ﻞﺣ ًﻼﻣﺎﻛ بﻮﺳر ﺎﺗ ﻢﻴﻨﻛ .
ﻪﺘﺴﻫ هراﻮﻳد ندﺮﺑ ﻦﻴﺑ زا ياﺮﺑ ﺮﻓﺎﺑ ﻦﻳا زا هدﺎﻔﺘﺳا
ﻲﻣﻢﻴﻨﻛ . رد ژﻮﻴﻔﻳﺮﺘﻧﺎﺳ2500 ﻪﺑ ﻪﻘﻴﻗد رد رود
تﺪﻣ10 ﻪﻘﻴﻗد.
6- ﻲﻣ ﻪﺘﺨﻳر رود ﻲﻳور ﻊﻳﺎﻣدﻮﺷ .25 وﺮﻜﻴﻣ
،هﺪﻨﻨﻛ ﺰﻴﻟ ﺮﻓﺎﺑ ﺮﺘﻴﻟ150 ﺮﺘﻴﻟوﺮﻜﻴﻣ
SDS
10
و ﺪﺻرد27 زﺎﻨﻴﺌﺗوﺮﭘ ﻢﻳﺰﻧآ ﺮﺘﻴﻟوﺮﻜﻴﻣ
K
) ﺎﺑ
ﺖﻈﻠﻏ5 ﺮﺘﻴﻟوﺮﻜﻴﻣ رد ﺪﺣاو ( ﻪﻠﺻﺎﺣ لﻮﻠﺤﻣ ﻪﺑ
تﺪﻣ ﻪﺑ و هﺪﺷ ﻪﻓﺎﺿا3 ﻋﺎﺳ يﺎﻣد رد ﺖ55
ﻲﻣ هداد راﺮﻗ مﺮﮔ بآ مﺎﻤﺣ رد داﺮﮕﻴﺘﻧﺎﺳ ﻪﺟرد-
ﺪﻴﻔﺳ يﺎﻫ لﻮﺒﻠﮔ ﻪﺘﺴﻫ تﺎﻳﻮﺘﺤﻣ ﻢﻀﻫ ﺎﺗ دﻮﺷ
ﻲﻣ ﻞﻣﺎﻛدﻮﺷ
7- ندﺮﻛ ﻪﻓﺎﺿا700 عﺎﺒﺷا ﻚﻤﻧ ﺮﺘﻴﻟوﺮﻜﻴﻣ )5/7
رﻻﻮﻣ ( تﺪﻣ ﻪﺑ ﺪﻳﺪﺷ ﺲﻜﺗرو و40-50 ﻪﻴﻧﺎﺛ
8- تﺪﻣ ﻪﺑ رﻮﺑﺰﻣ لﻮﻠﺤﻣ15 ﺖﻋﺮﺳ ﺎﺑ ﻪﻘﻴﻗد
3000 يﺎﻣد ﺎﺑ ﻪﻘﻴﻗد رد رود2 داﺮﮕﻴﺘﻧﺎﺳ ﻪﺟرد
ﻲﻣ ژﻮﻴﻔﻳﺮﺘﻧﺎﺳددﺮﮔ . نﻮﻜﻟﺎﻓ ﻪﺑ ﻲﻳور ﻊﻳﺎﻣ ﺲﭙﺳ
ﻲﻣ ﻞﻘﺘﻨﻣ ﺮﮕﻳدددﺮﮔ) .ﻦﻴﺌﺗوﺮﭘ ﻪﻠﺣﺮﻣ ﻦﻳا رد يﺎﻫ

يزروﺎﺸﻛ يژﻮﻟﻮﻨﻜﺗﻮﻴﺑ ﻪﻠﺠﻣ) هرود5 هرﺎﻤﺷ ،4 ،نﺎﺘﺴﻣز 1392(
١٠١
رد ﻚﻤﻧ ﺎﺑ هاﺮﻤﻫ ﻚﻴﺌﻠﻛﻮﻧ يﺎﻫﺪﻴﺳا ﻪﺑ ﻲﻟﺎﺼﺗا
ﻲﻣ ﻞﻴﻜﺸﺗ ﻲﮕﻧر ﺪﻴﻔﺳ بﻮﺳر ﻪﻟﻮﻟ يﺎﻬﺘﻧاﺪﻨﻫد(
9- و هدﺮﻛ ﻪﻓﺎﺿا ﻖﻠﻄﻣ لﻮﻧﺎﺗا ،لﻮﻠﺤﻣ ﻢﺠﺣ ﻢﻫ
ﻲﻣ نﺎﻜﺗ ﻲﻣارآ ﻪﺑﺗ ﻢﻴﻫددﻮﺷ طﻮﻠﺨﻣ ﺎ . ﻦﻳا رد
يﺎﻫ ﻪﺘﺷر ﻪﻠﺣﺮﻣ
DNA
ﻲﻣ ﺮﻫﺎﻇﺪﻧﻮﺷ . ﺲﭙﺳ
تﺪﻣ ﻪﺑ10 رد ﻪﻘﻴﻗد3000 ژﻮﻴﻔﻳﺮﺘﻧﺎﺳ رد رود
ﻲﻣﻢﻴﻨﻛ .
10- ﺪﻳﺪﺟ بﻮﻴﺗوﺮﻜﻴﻣ ﻪﺑ نﻮﻜﻟﺎﻓ يﺎﻬﺘﻧا بﻮﺳر
ﻲﻣ ﻞﻘﺘﻨﻣدﻮﺷ . لﻮﻧﺎﺗا ﺎﺑ رﺎﺑ ود ﻪﻠﺻﺎﺣ بﻮﺳر
ﻖﻠﻄﻣ75 %ﻲﻣ هداد ﻮﺸﺘﺴﺷ رد ًﺎﺘﻳﺎﻬﻧ و دﻮﺷ100
ﺮﺘﻴﻟوﺮﻜﻴﻣ
TE
)
EDTA
1 ،رﻻﻮﻣ ﻲﻠﻴﻣ
Tris-HCl
10 رﻻﻮﻣ ﻲﻠﻴﻣ (ﻲﻣ ﻞﺣددﺮﮔ.
11- مﺮﮔ بآ مﺎﻤﺣ رد نداد راﺮﻗ)55 ﻪﺟرد
داﺮﮕﻴﺘﻧﺎﺳ ( تﺪﻣ ﻪﺑ2 نﺪﺷ ﻞﺣ نﺎﻣز ﺎﺗ ﺖﻋﺎﺳ
ﻞﻣﺎﻛ
DNA
ﺮﻓﺎﺑ رد
TE
يﺎﻣد رد يراﺪﻬﮕﻧ و
20- هدﺎﻔﺘﺳا نﺎﻣز ﺎﺗ داﺮﮕﻴﺘﻧﺎﺳ ﻪﺟرد.
ﺖﻴﻔﻴﻛ و ﺖﻴﻤﻛ
DNA
ﺎﺑ هﺪﺷ جاﺮﺨﺘﺳا
ﺮﺘﻣﻮﺘﻓﻮﺘﻜﭙﺳا شور شور وﻓوﺮﺘﻜﻟارﻮ لژ يور ز
زرﺎﮔآ)1(% ﺪﺷ ﻦﻴﻴﻌﺗ. ﻪﺘﺷر ﺮﻴﺜﻜﺗ ﺖﻬﺟ درﻮﻣ ي
،ﺮﻈﻧ ﺶﻨﻛاو
PCR
ﻪﻠﻴﺳﻮﺑ داﻮﻣ صﻮﺼﺨﻣ
PCR
ﺖﻛﺮﺷ
GENNET
ﻪﺑ حﺮﺷﺮﻳز ﺎﺑ ﻲﻳﺎﻬﻧ ﺖﻈﻠﻏ
ﺶﻨﻛاو25 ﺮﺘﻴﻟوﺮﻜﻴﻣﺪﺷ مﺎﺠﻧا : ﺪﻳﺮﻠﻛ
ﻢﻳﺰﻴﻨﻣ)
MgCl2
(5/1 ﺮﺘﻴﻟوﺮﻜﻴﻣ)
Mm/µL
5/1( ،
ﺮﻓﺎﺑ)
PCR buffer
(5/2 ﺮﺘﻴﻟوﺮﻜﻴﻣ)1
X
( يﺎﻫزﺎﺑ ،
زآتﺎﻔﺴﻓ يﺮﺗ ﺪﻴﺗﻮﺌﻠﻛﻮﻧ ﻲﺴﻛوزد دا)
dNTPs
(2
ﺮﺘﻴﻟوﺮﻜﻴﻣ)
Mm/µL
8/0( ﺖﻓر ﺮﮔ زﺎﻏآ ،25/1
ﺮﺘﻴﻟوﺮﻜﻴﻣ)
pm/µL
5/0( ﺖﺸﮔﺮﺑ ﺮﮔزﺎﻏآ ،25/1
ﺮﺘﻴﻟوﺮﻜﻴﻣ)
pm/µL
5/0(ﻢﻳﺰﻧآ ،
DNA
زاﺮﻣ ﻲﻠﭘ
Taq
3/0 ﺮﺘﻴﻟوﺮﻜﻴﻣ)
unit/µL
25/0( ،
DNA
2
هﺰﻴﻧﻮﻳد بآ ،ﺮﺘﻴﻟوﺮﻜﻴﻣ
(dH2O)
2/14 ﺮﺘﻴﻟوﺮﻜﻴﻣ.
ﺶﻨﻛاو ﻲﻃﺎﻫﻲﻠﭘ يا هﺮﻴﺠﻧز ﺪﻨﭼ ي زاﺮﻣ ﺎﺑ
يﺎﻫﺮﮔزﺎﻏآ زا هدﺎﻔﺘﺳاهﺪﺷ ﻲﺣاﺮﻃ مﺮﻧ ﻂﺳﻮﺗ-
راﺰﻓا
Vector NTI
، ﻪﻌﻄﻗ ﻚﻳ654 يزﺎﺑ ﺖﻔﺟ
ﺪﻧﺎﺑ ﻦﻴﺌﺗوﺮﭘ نژ زا ﻪﺒﺷ ﺪﺷر رﻮﺘﻛﺎﻓ ﻪﺑ هﺪﻧﻮﺷ
ﻦﻴﻟﻮﺴﻧا ﭗﻴﺗ3 ﺎﻫﺮﮔزﺎﻏآ ﻲﻟاﻮﺗ ﻪﻛ ﺪﺷ ﺮﻴﺜﻜﺗﺑ ﻪ
ﻲﻣ ﺮﻳز حﺮﺷﺪﺷﺎﺑ:
ﺖﻓر ﺮﮔ زﺎﻏآ )
Forward primer
:(
CCAAGCGTGAGACAGAATAC-3`
5`-
،
ﺖﺸﮔﺮﺑ ﺮﮔزﺎﻏآ )
Reverse primer
:(
5`-AGGAGGGATAGGAGCAAGAT-3`
.
ﻌﻄﻗ ﺮﻴﺜﻜﺗتﺎ زا هدﺎﻔﺘﺳا ﺎﺑ ﺮﻈﻧ درﻮﻣ
عﻮﻧ ﺮﻠﻜﻳﺎﺳﻮﻣﺮﺗ هﺎﮕﺘﺳد
TECHNE
ﻂﻳاﺮﺷ ﺎﺑ
ﺪﺷ مﺎﺠﻧا ﺮﻳز ﻲﻳﺎﻣد: 1 (يزﺎﺳ ﺖﺷﺮﺳاو عوﺮﺷ
ﻪﻴﻟوا
DNA
:
3 ﻪﻘﻴﻗد،
C°
95 )ﻚﻳ ﻪﺧﺮﭼ(. 2 (
يزﺎﺳ ﺖﺷﺮﺳاو نﺪﺷ
DNA
:45 ﺎﺛﻪﻴﻧ ،
C°
95 ،
ﺮﮔزﺎﻏآ لﺎﺼﺗا ﻪﺑ
DNA
:40 ﻪﻴﻧﺎﺛ
C°
60 ،ﻂﺴﺑ :
50 ﻪﻴﻧﺎﺛ
C°
72 )35 ﻪﺧﺮﭼ .(3 (ﻂﺴﺑ ﻞﻴﻤﻜﺗ :
10ﻪﻘﻴﻗد
C°
72 )ﻚﻳ ﻪﺧﺮﭼ.(
تﻻﻮﺼﺤﻣ زرﻮﻓوﺮﺘﻜﻟا
PCR
لژ يور
زرﺎﮔآ2 ژﺎﺘﻟو ﺎﺑ ﺪﺻرد88 ﺖﻟو تﺪﻣ و30 ﻪﻘﻴﻗد
يﺰﻴﻣآ ﮓﻧر زا ﺲﭘ ﻪﻃﻮﺑﺮﻣ لژ و ﺖﻓﺮﮔ ترﻮﺻ
ﻣ ﻪﻌﺷا ﺮﻳز ﺪﻳﺎﻣوﺮﺑ مﻮﻳﺪﻴﺗا ﺎﺑ ﻲﺳرﺮﺑ ﺶﻔﻨﺑ ياروﺎ
ﺪﻧﺪﺷ . ﺶﻨﻛاو مﺎﺠﻧا رﻮﻈﻨﻣ ﻪﺑ
SSCP
ﻪﺘﺷر ، يﺎﻫ
DNA
هدﺎﻔﺘﺳا ﺎﺑ هﺪﺷ ﺮﻴﺜﻜﺗ زا
SSCP dye
ﻪﺑ
ﺖﺒﺴﻧ16 ﻪﺑ4 ﺪﻣ ﻪﺑ نﺎﻣز ت10 يﺎﻣد رد ﻪﻘﻴﻗد
96 ﻲﺘﻧﺎﺳ ﻪﺟردداﺮﮔ ﻚﺗﻪﺘﺷر ﻪﻛ ﺪﻧﺪﺷ ﻲﻳا

دﺎﺷ ﻲﻧﺎﺧ ﻲﺿﺎﻗ ،نارﺎﻜﻤﻫ و1392
١٠٢
راﺮﻗ ﺦﻳ ﻞﺧاد رد ﻪﻠﺣﺮﻣ ﻦﻳا زا ﺲﭘ ﻪﻠﺻﺎﻓﻼﺑ
ﺪﻨﺘﻓﺮﮔ. زرﻮﻓوﺮﺘﻜﻟا ﻪﺘﺷر ﻚﺗ تﻻﻮﺼﺤﻣ هﺪﺷ ﻲﻳا
ﺪﻴﻣآ ﻞﻳﺮﻛا لژ يور 13 ژﺎﺘﻟو ﺎﺑ ﺪﺻرد
V
280 ﻪﺑ
تﺪﻣ19 ﺖﻋﺎﺳ ياﺮﺑ ﻲﺳرﺮﺑ ﻲﻠﻜﺷ ﺪﻨﭼ ﺎﻫ مﺎﺠﻧا
و هﺮﻘﻧ تاﺮﺘﻴﻧ زا هدﺎﻔﺘﺳا ﺎﺑ لژ يﺰﻴﻣآ ﮓﻧر
ﺖﻓﺮﮔ ترﻮﺻ.
ﻞﻜﺷ1- ﻪﻌﻄﻗ654 ﺮﻴﺜﻜﺗ زا ﻞﺻﺎﺣ يزﺎﺑ ﺖﻔﺟ نوﺰﮔا3 نژ
IGFBP3
زرﺎﮔآ لژ يور 2%.
ﻞﻜﺷ2- يﺎﻫﻮﮕﻟاﭗﻴﺗﻮﻧژ يدﻮﻤﻋ زرﻮﻓوﺮﺘﻜﻟا زا ﻞﺻﺎﺣ)
SSCP
( نژ
IGFBP3
.
هزاﺪﻧاﻲﻧﻮﺧ يﺎﻫﺮﺘﻣارﺎﭘ يﺮﻴﮔ
ﺪﻳﺮﺴﻴﻠﮔ يﺮﺗ
ياﺮﺑ ﺘﻴﻛ زا ﺪﻳﺮﺴﻴﻠﮔ يﺮﺗ يﺮﻴﮔ هزاﺪﻧا يﺎﻬ
ﺪﺷ هدﺎﻔﺘﺳا نﻮﻣزآ سرﺎﭘ ﺖﻛﺮﺷ . ﺶﻳﺎﻣزآ ﻦﻳا رد
زا زﺎﭙﻴﻟ ﻦﻴﺌﺗوﺮﭘﻮﭙﻴﻟ ﻢﻳﺰﻧآ ﻂﺳﻮﺗ لوﺮﺴﻴﻠﮔ اﺪﺘﺑا
ﻞﺣاﺮﻣ ﻲﻃ ﺲﭙﺳ و هﺪﺷ اﺪﺟ بﺮﭼ يﺎﻫﺪﻴﺳا
اﺮﭘ ،ﺮﻳز ﺎﺑ لوﺮﺴﻴﻠﮔ زا هﺪﺷ دازآ نژورﺪﻴﻫ ﺪﻴﺴﻛ
4- ﻢﻳﺰﻧآ تروﺎﺠﻣ رد لﻮﻨﻓ و ﻦﻳﺮﻴﭘ ﻲﺘﻧآ ﻮﻨﻴﻣآ

bp
يﻮﮕﻟا
3
يﻮﮕﻟا
2
ﻮﮕﻟا
ي
1
يﻮﮕﻟا
4
يﻮﮕﻟا
5
٠٠
bp

يزروﺎﺸﻛ يژﻮﻟﻮﻨﻜﺗﻮﻴﺑ ﻪﻠﺠﻣ) هرود5 هرﺎﻤﺷ ،4 ،نﺎﺘﺴﻣز 1392(
١٠٣
ﻲﻣ ﻦﻴﻤﻴﻧﻮﻨﻴﻛ ﻞﻴﻜﺸﺗ زاﺪﻴﺴﻛاﺮﭘﺪﻫد . ناﺰﻴﻣ
ﻚﻳﺮﺘﻣﻮﺘﻓ ترﻮﺻ ﻪﺑ ﻪﻛ هﺪﺷ ﻞﻴﻜﺸﺗ ﻦﻴﻤﻴﻧﻮﻨﻴﻛ
ﺪﻳﺮﺴﻴﻠﮔ يﺮﺗ راﺪﻘﻣ ﺎﺑ ﺖﺳا يﺮﻴﮔ هزاﺪﻧا ﻞﺑﺎﻗ
دراد ﻢﻴﻘﺘﺴﻣ ﻪﻄﺑار. لﻮﻣﺮﻓ ﻖﺒﻃ ﺪﻳﺮﺴﻴﻠﮔ يﺮﺗ يﺎﻫ
ﻟورﺪﻴﻫ ﺮﻳز لﻮﻃ رد ﺖﻳﺎﻬﻧ رد و هﺪﺷ ﺰﻴﻟﺎﺗﺎﻛ و ﺰﻴ
جﻮﻣ546 ﺮﺘﻣﻮﻧﺎﻧ )546 -500 ﺮﺘﻣﻮﻧﺎﻧ (هزاﺪﻧا يﺮﻴﮔ
ﺪﺷ.
لوﺮﺘﺴﻠﻛ
ﺖﻴﻛ زا لوﺮﺘﺴﻠﻛ يﺮﻴﮔ هزاﺪﻧا ياﺮﺑ يﺎﻫ
ﺪﺷ هدﺎﻔﺘﺳا نﻮﻣزآ سرﺎﭘ ﺖﻛﺮﺷ . ﺶﻳﺎﻣزآ ﻦﻳا رد
ﺰﻴﻟورﺪﻴﻫ ﻪﺠﻴﺘﻧ رد هﺪﺷ ﺪﻴﻟﻮﺗ نژورﺪﻴﻫ ﺪﻴﺴﻛاﺮﭘ
و لﻮﻨﻓ هاﺮﻤﻫ ﻪﺑ ،لوﺮﺘﺴﻠﻛ نﻮﻴﺳاﺪﻴﺴﻛا و4-
زاﺪﻴﺴﻛاﺮﭘ ﻢﻳﺰﻧآ تروﺎﺠﻣ رد ﻦﻳﺮﻴﭘ ﻲﺘﻧآ ﻮﻨﻴﻣآ
ﻲﻣ ﻦﻴﻤﻴﻧﻮﻨﻴﻛ ﻞﻴﻜﺸﺗﺪﻫد . ﻦﻴﻤﻴﻧﻮﻨﻴﻛ ناﺰﻴﻣ
هزاﺪﻧا ﻞﺑﺎﻗ ﻚﻳﺮﺘﻣﻮﺘﻓ ترﻮﺻ ﻪﺑ ﻪﻛ هﺪﺷ ﻞﻴﻜﺸﺗ-
دراد ﻢﻴﻘﺘﺴﻣ ﻪﻄﺑار لوﺮﺘﺴﻠﻛ راﺪﻘﻣ ﺎﺑ ﺖﺳا يﺮﻴﮔ.
لوﺮﺘﺴﻠﻛ لﻮﻣﺮﻓ ﻖﺒﻃ و ﺰﻴﻟورﺪﻴﻫ ﺮﻳز يﺎﻫ
جﻮﻣ لﻮﻃ رد ﺖﻳﺎﻬﻧ رد و هﺪﺷ ﺰﻴﻟﺎﺗﺎﻛ546
ﺮﺘﻣﻮﻧﺎﻧ )546-500 ﺮﺘﻣﻮﻧﺎﻧ (هزاﺪﻧاﻲﻣ يﺮﻴﮔﺪﺷ .
Cholesterol ester+ H
2
O
Cholesterol + Fatty acid
Cholesterol + O
2
Cholesterol -3-one+ H
2
O
2
2H
2
O
2
+4-Aminoantipyrine+phenol
Quinoneimine+4H
2
O
شور ﻪﻳﺰﺠﺗ يرﺎﻣآ
ﭘياﺮﺑ يﺪﻧﺎﺑ يﻮﮕﻟا ﻦﻴﻴﻌﺗ زا ﺲ ﻚﺗ ﻚﺗ
ﺎﻬﻣاد و ﻪﺒﺳﺎﺤﻣ ﻲﻧاواﺮﻓﻮﮕﻟا ﺎﻫ هﺎﮕﻳﺎﺟ ياﺮﺑ
ﻲﺳرﺮﺑ درﻮﻣ )وﺰﮔان 3(، هدادﻃﻮﺑﺮﻣ يﺎﻫﻪ)و نز
ﻪﺷﻻ هدزﺎﺑ ،مﺮﮔ ﻪﺷﻻ نزو ،هﺪﻧز ﻲﺑﺮﭼ ﺖﻣﺎﺨﺿ ،
،هدﺰﻴﺳ و هدزاود هﺪﻧد ﻦﻴﺑ ﺖﺸﭘ مﺎﺧ ﻦﻴﺌﺗوﺮﭘ
،ﺖﺷﻮﮔ ﻠﮔ يﺮﺗنﻮﺧ لوﺮﺘﺴﻠﻛ ،نﻮﺧ ﺪﻳﺮﺴﻴ،
بﺮﭼ يﺎﻫ ﺪﻴﺳا ) رد حﺮﺷ لوﺪﺟ4( ، و ﻦﺳ
ﺲﻨﺟ مادﻪﻣﺎﻧﺮﺑ دراو
Excel
ﺶﻳاﺮﻳو زا ﺲﭘ و،
ﻪﻣﺎﻧﺮﺑ دراو
SAS
و هﺪﺷﺎﺑ ﻪﻳور
GLM
درﻮﻣ
ﻪﻳﺰﺠﺗ ﺪﻨﺘﻓﺮﮔ راﺮﻗ ﻞﻴﻠﺤﺗ و . ﺮﺛا ﺶﻳﺎﻣزآ ﻦﻳا رد
ﭗﻴﺗﻮﻧژ يﺎﻫ
IGFBP3
و ناﻮﻴﺣ ﺲﻨﺟ و ﻦﺳ ﺮﺛا
نزو ﺮﺛا و ﺖﺑﺎﺛ ﻞﻣﺎﻋ ناﻮﻨﻋ ﻪﺑﻨﻫمﺎﮕ ماد رﺎﺘﺸﻛ
ﻪﺑ ناﻮﻨﻋ ﺪﺷ هداد راﺮﻗ لﺪﻣ رد ﺖﻳراوﻮﻛ ) ضﺮﻓ
ﺮﻈﻧ رد هﺮﻴﺟ و ﻲﺷروﺮﭘ ﻂﻳاﺮﺷ ندﻮﺑ نﺎﺴﻜﻳ
ﺪﺷ ﻪﺘﻓﺮﮔ( .لﺪﻣ هدﺎﻔﺘﺳا درﻮﻣترﻮﺼﺑ ﺮﻳزدﻮﺑ:
y
ijkl =
µ
+
A
i
+ G
j
+D
k
+ b(W
ijkl
-
W
) +
(
AG
)
ij
+e
ijkl
لﺪﻣ ﻦﻳا رد ﻪﻛ
y
ijklo
،
ﺮﻫ طﻮﺑﺮﻣ تاﺪﻫﺎﺸﻣ زا ﻚﻳ
تﺎﻔﺻ ﻪﺑ، µ ﻦﻴﮕﻧﺎﻴﻣ رد ﺖﻔﺻﺟﺖﻴﻌﻤ،
A
i
ﺮﺛا
i
يﺮﻴﮕﻧﻮﺧ مﺎﮕﻨﻫ رد ناﻮﻴﺣ ﻦﺳ ﻦﻴﻣا ) رﻮﻄﺑ ﻦﺳ
ناﺪﻧد شرﺎﻤﺷ زا ﻲﺒﻳﺮﻘﺗﺪﺷ ﻪﺒﺳﺎﺤﻣ ﺎﻫ(،
G
j
ﺮﺛا
j
CHE
CHO
POD

دﺎﺷ ﻲﻧﺎﺧ ﻲﺿﺎﻗ ،نارﺎﻜﻤﻫ و1392
١٠٤
ﭗﻴﺗﻮﻧژ ﻦﻴﻣا،
D
k
ﺮﺛا
K
ﻦﻴﻣا ﺲﻨﺟ،
b
ﺐﻳﺮﺿ
ﺖﻴﻌﺑﺎﺗ تﺎﻔﺻ ﺮﺑ يور نزو مﺎﮕﻨﻫ و يﺮﻴﮕﻧﻮﺧ
ماد رﺎﺘﺸﻛ ،
W
ijkl
نزو ﺮﻫ ماديﺮﻴﮕﻧﻮﺧ مﺎﮕﻨﻫ ،
W
ماد نزو ﻦﻴﮕﻧﺎﻴﻣيﺮﻴﮕﻧﻮﺧ مﺎﮕﻨﻫ ﺎﻫ ،
(AG)
ij
ناﻮﻴﺣ ﻦﺳ و ﭗﻴﺗﻮﻧژ ﻞﺑﺎﻘﺘﻣ ﺮﺛا و
e
ijkl
ﻞﻣاﻮﻋ ﺮﺛا
ﻲﻓدﺎﺼﺗهﺪﻧﺎﻤﻴﻗﺎﺑ دﻮﺑ.
ﺞﻳﺎﺘﻧ
ﻲﻧاواﺮﻓ يﺎﻫﺎﻫﻮﮕﻟا لوﺪﺟ رد1 ﺖﺳا هﺪﺷ هدروآ.
يﺎﻫﻮﮕﻟا ژﻲﭙﻴﺗﻮﻧ 1 ،2 ،3، 4 و5 نژ ﻪﺑ طﻮﺑﺮﻣ
IGFBP3
ﺮﺗ ﻪﺑ يﺎﻫ ﻲﻧاواﺮﻓ ﺎﺑ ﺐﻴﺗ67/66 ،33/3 ،
67/6، 33/13 و10 ﺪﺻردﺪﺷ هﺪﻫﺎﺸﻣﺪﻧ .
ﻪﻈﺣﻼﻣﻲﻣ ﻲﻧاواﺮﻓ ﻦﻳﺮﺘﺸﻴﺑ ﻪﻛ دﻮﺷ ﻪﺑ طﻮﺑﺮﻣ
يﻮﮕﻟا ﻲﻣ لواﺪﺷﺎﺑ.
لوﺪﺟ1 - ﻲﻧاواﺮﻓ ﺎﻫﻮﮕﻟاي
SSCP
نوﺰﮔا3 نژ
IGFBP3
رد ناﺪﻨﻔﺳﻮﮔ داﮋﻧ لز.
Table 2: The pattern frequency of ovine IGFBP3 gene in Zel sheep
ﻮﮕﻟا
ﺎﻫ
(Pattern)
ﻲﻧاواﺮﻓ
(%)
(Frequency)
1
66.67
2
3.33
3
6.67
4
13.33
5
10
ﻪﻛ رﻮﻄﻧﺎﻤﻫﻔﺻ ﺲﻧﺎﻳراو ﺰﻴﻟﺎﻧآ ﺞﻳﺎﺘﻧﺎ رد ﻪﺷﻻ ت
لوﺪﺟ2
نﺎﺸﻧﻲﻣﺪﻫد ﻦﻴﺑ ﻪﻄﺑار ﻮﮕﻟا يﺎﻫ
IGFBP3
و مﺮﮔ ﻪﺷﻻ نزو تﺎﻔﺻ )
P<0.05
( ،
ﺖﺸﭘ ﻲﺑﺮﭼ ﺖﻣﺎﺨﺿ )
P<0.01
( مﺎﺧ ﻦﻴﺌﺗوﺮﭘ و
ﺖﺷﻮﮔ )
P<0.01
( و راد ﻲﻨﻌﻣﺎﺑ تﺎﻔﺻ ﺮﻳﺎﺳ
ﻪﻄﺑارﺪﻨﺘﺷاﺪﻧ يراد ﻲﻨﻌﻣ )
P>0.05
.(
ﺮﮕﻳد يﻮﺳ زا ﺲﻧﺎﻳراو ﺰﻴﻟﺎﻧآ ﺞﻳﺎﺘﻧﻪﺠﻨﺳاﺮﻓ يﺎﻫ
ﻲﻧﻮﺧ ﻪﻛ لوﺪﺟ رد2 ،ﺖﺳا هﺪﺷ ﻪﺋاراﻲﻣ نﺎﺸﻧ
دا ﻪﻛ د ﻦﻴﺑﻮﮕﻟاﻒﻠﺘﺨﻣ يﺎﻫ نژ
IGFBP3
و يﺮﺗ
نﻮﺧ لوﺮﺘﺴﻠﻛ و ﺪﻳﺮﺴﻴﻠﮔ طﺎﺒﺗرا راد ﻲﻨﻌﻣي
ﺟودﻮ دراﺪﻧ. )
P>0.05
.( ﻦﻴﻨﭽﻤﻫ ﺰﻴﻟﺎﻧآ ﺞﻳﺎﺘﻧ
بﺮﭼ يﺎﻫﺪﻴﺳا تﺎﺒﻴﻛﺮﺗ ﺲﻧﺎﻳراو ﺰﻴﻧ رد ﻪﻛ
لوﺪﺟ3 ﻲﻣ نﺎﺸﻧ ،ﺖﺳا هﺪﺷ ﻪﺋارا ﺪﻫد ﻪﻛ ﺮﺛا
ﻮﮕﻟا يﺎﻫ
IGFBP3
ﺪﻴﺳا راﺪﻘﻣ ﺮﺑ ﺎﻬﻨﺗ
ﻚﻴﺋﻮﻧﺎﻛدﺎﺘﭙﻫ )
C17:0
(ﺖﺳا هﺪﺷ راد ﻲﻨﻌﻣ
)
P<0.01
( يﺎﻫﺪﻴﺳا ﺮﮕﻳد يور ﺮﺑ وبﺮﭼ ﻲﻨﻌﻣ
ﺖﺴﻴﻧ راد.

يزروﺎﺸﻛ يژﻮﻟﻮﻨﻜﺗﻮﻴﺑ ﻪﻠﺠﻣ) هرود5 هرﺎﻤﺷ ،4 ،نﺎﺘﺴﻣز 1392(
١٠٥
لوﺪﺟ2- تﺎﻌﺑﺮﻣ ﻞﻗاﺪﺣ ﻦﻴﮕﻧﺎﻴﻣ )
LSM±SE
(ﻪﺠﻨﺳاﺮﻓ و ﻪﺷﻻ تﺎﻔﺻ ﻲﻧﻮﺧ يﺎﻫ ونآ طﺎﺒﺗراﺎﺑ ﺎﻫ
يﺎﻫﻮﮕﻟا
SSCP
نژ
3
IGFBP ناﺪﻨﻔﺳﻮﮔ رد لز داﮋﻧ.
Table 2- Least square means (LSM) of carcass traits and blood parameters and its
associations with
IGFBP3 gene’s patterns in Zel breed sheep.
ﺎﻔﺻ
ت
(Traits)
ﺎﻫﻮﮕﻟا
(Patterns)
يﻮﮕﻟا
1
(Pattern1)
يﻮﮕﻟا
2
(Pattern2)
يﻮﮕﻟا
3
(Patern3)
يﻮﮕﻟا
4
(Pattern4)
يﻮﮕﻟا
5
(Pattern5)
رﺎﺘﺸﻛ نزو
n.s
Slaughter Weight
8.19±37.28
3.34±33.28
9.20±44.04
5.27±26.77
10.81±28.41
نزو
ﻪﺷﻻ
مﺮﮔ
*
Carcass Weight
1.84±13.25
ab
0.75±15.87
a
2.13±10.84
b
1.29±16.34
a
2.45±11.82
b
هدزﺎﺑ
ﻪﺷﻻ
n.s
Carcass Efficiency
5.37±37.95 2.21±44.70 6.22±33.68 3.38±46.72 1.42±4.13
ﺖﻣﺎﺨﺿ
ﻲﺑﺮﭼ
ﺖﺸﭘ
**
back fat thickness
1.06±2.78
ab
43.0±2.27
bc
0.23±1.17
c
0.74±2.47
ab
1.42±4.13
a
ﻦﻴﺌﺗوﺮﭘ
مﺎﺧ
**
Crude Protein
1.19±215.81
a
0.49±217.98
a
1.38±215.03
a
0.83±217.52
a
1.59±176.37
b
يﺮﺗ
ﺪﻳﺮﺴﻴﻠﮔ
n.s
(triglyceride)
2.12±12.33 4.38±38.80 15.60±37.15 8.53±43.30 2.97±15.63
لوﺮﺘﺴﻠﻛ
n.s
(cholesterol)
28.48±93.67 11.24±67.54 36.63±92.50 20.05±74.36 36.71±62.67
ﺮﻴﻏ ﻦﻴﺗﻻ فوﺮﺣ ﺪﺷﺎﺑ ﻲﻣ راد ﻲﻨﻌﻣ فﻼﺘﺧا دﻮﺟو ﺮﮕﻧﺎﻴﺑ ﺮﻄﺳ ﺮﻫ رد ﻪﺑﺎﺸﻣ
)
ns
:
،راد ﻲﻨﻌﻣ ﺮﻴﻏ
*
و
**
ﺢﻄﺳ رد راد ﻲﻨﻌﻣ ﺐﻴﺗﺮﺗ ﻪﺑ
05
/
0
و
01
/
0
(

دﺎﺷ ﻲﻧﺎﺧ ﻲﺿﺎﻗ ،نارﺎﻜﻤﻫ و1392
١٠٦
لوﺪﺟ3 - تﺎﻌﺑﺮﻣ ﻞﻗاﺪﺣ ﻦﻴﮕﻧﺎﻴﻣ
)
LSM±SE
(
نآ طﺎﺒﺗرا و بﺮﭼ يﺎﻫﺪﻴﺳا ﻞﻳﺎﻓوﺮﭘ يﺎﻫﻮﮕﻟا ﺎﺑ ﺎﻫ
SSCP
نژ
3
IGFBP لز داﮋﻧ ناﺪﻨﻔﺳﻮﮔ رد.
Table 4- Least Ssquare Means (LSM) of fatty acids profiles and its association with
IGFBP3 gene’s patterns in Zel breed sheep.
ﺪﻴﺳا
ﻫ
يﺎ
بﺮﭼ
(Fatty Acids)
ﺎﻫﻮﮕﻟا
(Patterns)
ﻮﮕﻟا
ي
1
(Pattern1)
ﻮﮕﻟا
ي
2
(Pattern2)
ﻮﮕﻟا
ي
3
(Pattern3)
ﻮﮕﻟا
ي
4
(Pattern4)
ﻮﮕﻟا
ي
5
(Pattern5)
ﻚﻴﺘﺴﻳﺮﻣ
ﺪﻴﺳا
(C14:0)
2.62±1.05
3.16±0.43
2.81±1.18 3.04±0.68
3.51±1.39
ﺪﻴﺳا
ﻚﻴﺘﻴﻤﻟﺎﭘ
(C16:0)
26.79±3.33 26.49±1.36 17.85±3.74 26.82±2.14 30.82±4.39
ﺪﻴﺳا
ﻚﻴﺌﻟﻮﺘﻴﻤﻟﺎﭘ
(C16:1)
6.15±1.39 4.52±0.56 3.80±1.56 3.53± 0.89 3.94±1.83
ﺪﻴﺳا
ﻚﻴﺋﻮﻧﺎﻛدﺎﺘﭙﻫ
** (C17:0)
2.01±1.08
b
2.01±0.44
b
7.49±1.21
a
2.61±0.69
b
3.24±1.42
ab
ﺪﻴﺳا
ﻚﻴﺋاﻮﻧﺎﻛدﺎﺘﭙﻫ
(C17:1)
0.30±0.35
0.75± 0.14 0.87±0.40 0.62± 0.22 0.34±0.47
ﺪﻴﺳا
ﻚﻳرﺎﺌﺘﺳا
(C18:0)
19.31±3.16 15.94±1.29 13.76±3.55 17.87±2.04 14.03±4.17
ﺪﻴﺳا
ﻚﻴﺌﻟوا
(C18:1)
36.27±3.37 40.45±1.37 43.25±3.79 39.70± 2.17 40.49±4.45
ﺪﻴﺳا
ﻚﻴﺌﻟﻮﻨﻴﻟ
(C18:2)
5.85± 1.61 5.37± 0.65 3.01± 1.80
6.16± 1.03 7.20± 2.12
ﺪﻴﺳا
ﻚﻴﺌﻟﻮﻨﻴﻟ
(C18:3)
0.27±0.27 0.24±0.11 0.26±0.09 0.22±0.17 0.34±0.36
ﺪﻴﺳا
ﻚﻴﺋﻮﻧااﺮﺘﺗازﻮﻜﻳا
(C20:4)(n-6)
0.88±0.64
0.77±0.26 0.28 ±0.72 1.14 ±0.41 1.46 ±0.84
ﺪﻴﺳا
ﺎﺘﻨﭘازﻮﻜﻳا
ﻚﻴﺋﻮﻧا
( C20:5)(n-3)EPA
0.27 ±0.27 0.23 ±0.11 0.20 ±0.31 0.23 ±0.17 0.46 ±0.36
ﺪﻴﺳا
اﺰﮕﻫازﻮﻜﻳا
ﻚﻴﺋﻮﻧا
(C22:6)(n-3)DHA
0.30 ±0.19 0.23 ±0.07 0.16 ±0.21
0.25 ±0.12 0.36 ±0.25
يﺎﻫﺪﻴﺳا
بﺮﭼ
عﺎﺒﺷا
( SFA)
73.09 ±3.84 77.18±1.56 82.85±4.31 74.30±2.47 75.30±5.07
يﺎﻫﺪﻴﺳا
بﺮﭼ
ﺎﺑ
ﺪﻨﭼ
ﺪﻧﻮﻴﭘ
ﻪﻧﺎﮔود (MUFA)
41.66±6.12 45.50±2.49 54.67±6.87 41.81±3.94 40.72±8.07
يﺎﻫﺪﻴﺳا
بﺮﭼ
ﺎﺑ
ﻚﻳ
ﺪﻧﻮﻴﭘ
ﻪﻧﺎﮔود
(PUFA)
7.28±2.35 6.63±0.95 7.21±2.63 7.57±1.51 10.29±3.09
ﺖﺒﺴﻧ
يﺎﻫﺪﻴﺳا
بﺮﭼ
ﺎﺑ
ﺪﻨﭼ
ﺪﻧﻮﻴﭘ
ﻪﻧﺎﮔود ﻪﺑ يﺎﻫﺪﻴﺳا بﺮﭼ عﺎﺒﺷا
(PUFA/SFA)
0.09±0.03 0.08±0.01 0.08±0.03 0.10±0.02 0.13±0.04
ﺖﺒﺴﻧ
ﺪﻴﺳا
ﻚﻴﺌﻟﻮﻨﻴﻟ
ﻪﺑ
ﺪﻴﺳا
ﻚﻴﻨﻟﻮﻨﻴﻟ (n6/n3)
7.97±4.39 9.72±1.79 8.81±4.92 12.64±2.82 7.78±5.78
اد ﻲﻨﻌﻣ فﻼﺘﺧا دﻮﺟو ﺮﮕﻧﺎﻴﺑ ﺮﻄﺳ ﺮﻫ رد ﻪﺑﺎﺸﻣ ﺮﻴﻏ ﻦﻴﺗﻻ فوﺮﺣﺪﺷﺎﺑ ﻲﻣ ر
)
ns
:
،راد ﻲﻨﻌﻣ ﺮﻴﻏ
*
و
**
ﺢﻄﺳ رد راد ﻲﻨﻌﻣ ﺐﻴﺗﺮﺗ ﻪﺑ
05
/
0
و
01
/
0
(

يزروﺎﺸﻛ يژﻮﻟﻮﻨﻜﺗﻮﻴﺑ ﻪﻠﺠﻣ) هرود5 هرﺎﻤﺷ ،4 ،نﺎﺘﺴﻣز 1392(
١٠٧
ﺚﺤﺑ يﺮﻴﮔ ﻪﺠﻴﺘﻧ و
ﺶﻨﻛاو ﻲﻃ
PCR
ﻪﻌﻄﻗ ،654 يزﺎﺑ ﺖﻔﺟ
زا نژ ﻪﺑ هﺪﻧﻮﺷ ﺪﻧﺎﺑ ﻦﻴﺌﺗوﺮﭘ ﻪﺒﺷ ﺪﺷر رﻮﺘﻛﺎﻓ
ﻦﻴﻟﻮﺴﻧا)
IGFBP3
( ﺪﺷ ﺮﻴﺜﻜﺗ) ﻞﻜﺷ1 .( ﺲﭙﺳ
يﺎﻫﻮﮕﻟايﺪﻧﺎﺑ زا ﻞﺻﺎﺣ
SSCP
ﺎﻫ ﻪﻧﻮﻤﻧ) ﻞﻜﺷ
2 (ﺨﺸﻣﺪﺷ ﺺ) يﺎﻫﻮﮕﻟا1 ﺎﺑ3 و2 ﺎﺑ4 رد
ﻲﻣ ﺮﻈﻨﺑ ﻪﺒﺷ ﻢﻫ ﻪﺑ ﺎﺘﺒﺴﻧ ﺮﻳﻮﺼﺗ ﺖﻠﻋ ﻪﺑ ﻪﻛ ﺪﻨﻳآ
ﻲﻣ يرادﺮﺒﺴﻜﻋ ﺖﻴﻔﻴﻛ مﺪﻋﺪﺷﺎﺑ( . ﻪﻛ رﻮﻄﻧﺎﻤﻫ
ﻲﻣ هﺪﻫﺎﺸﻣ دﻮﺷيﻮﮕﻟا 1 ﺎﺑ67/66 ﺪﺻرد
داد صﺎﺼﺘﺧا دﻮﺧ ﻪﺑ ار ﻲﻧاواﺮﻓ ﻦﻳﺮﺘﺸﻴﺑﻮﺑ هد.
يﺎﻫﻮﮕﻟا
IGFBP3
مﺮﮔ ﻪﺷﻻ نزو تﺎﻔﺻ ﺎﺑ
)
P<0.05
(ﺸﭘ ﻲﺑﺮﭼ ﺖﻣﺎﺨﺿ ،ﺖ )
P<0.01
( و
ﺖﺷﻮﮔ مﺎﺧ ﻦﻴﺌﺗوﺮﭘ )
P<0.01
(ﻨﭽﻤﻫ و ﻦﻴ
ﺪﻴﺳا ﺐﻴﻛﺮﺗ ﻚﻴﺋﻮﻧﺎﻛدﺎﺘﭙﻫ بﺮﭼ)
P<0.01
(
ﺪﻧداد نﺎﺸﻧ يراد ﻲﻨﻌﻣ طﺎﺒﺗرا. ﺎﺑ ﻲﺸﻫوﮋﭘ رد
نژ ﻪﻌﻟﺎﻄﻣ
IGFBP3
) ﻲﻳﺎﻬﺘﻧا يﺎﻬﺸﺨﺑ ﻞﻣﺎﺷ
نوﺰﮔا2 نوﺮﺘﻨﻳا ﻞﻛ ،2 نوﺰﮔا ،3 زا ﻲﺸﺨﺑ و
نوﺮﺘﻨﻳا3 ( يور ﺮﺑ4 ناﺪﻨﻔﺳﻮﮔ زا داﮋﻧ
)
Rahmani
Ossimi, Awassi, Barki
(
ﺮﺼﻣ رد
ﻚﻴﻨﻜﺗ زا هدﺎﻔﺘﺳا ﺎﺑ
PCR-RFLP
ﻪﻧﻮﮔ ﺮﻫ ناﺪﻘﻓ
ﺮﻫ رد ار ﻲﻜﻴﺘﻧژ ﻢﺴﻴﻓرﻮﻣ ﻲﻠﭘ4 شراﺰﮔ داﮋﻧ
ﻦﻴﻤﻫ ﺰﻴﻧ ﺪﻨﻫ ناﺪﻨﻔﺳﻮﮔ يﺎﻫداﮋﻧ رد ﻪﻛ ﺪﻧدﻮﻤﻧ
ﺖﺳا هﺪﺷ شراﺰﮔ ﺞﻳﺎﺘﻧ
(Ali et al.., 2009)
.
ﻦﻳا رد ار ﻲﻤﺴﻴﻓرﻮﻣ ﻲﻠﭘ ﻪﻧﻮﮕﭽﻴﻫ ﻦﻴﻨﭽﻤﻫ
رد ﻲﻧژ هﺎﮕﻳﺎﺟ5 ﺳﻮﮔ داﮋﻧ شراﺰﮔ ﺪﻨﻫ رد ﺪﻨﻔ
ﭗﻴﺗﻮﻧژ ﻚﻳ ﺎﻬﻨﺗ و ﺪﻧدﺮﻜﻧ
AA
ﺸﻣ ار هﺪﻫﺎ
ﺪﻧدﻮﻤﻧ
2006)
(Kumar et al.
. ﺮﮕﻳد ﻲﺸﻫوﮋﭘ رد
نژ ﻪﻌﻟﺎﻄﻣ ﺎﺑ
IGFBP3
ﺪﻨﻔﺳﻮﮔ داﮋﻧ رد
Muzaffarnagari
يدﺎﺼﺘﻗا تﺎﻔﺻ ﺎﺑ نآ طﺎﺒﺗرا و
ﻮﻣ ﻲﻠﭘ هﺪﻫﺎﺸﻣ هﺎﮕﻳﺎﺟ ﻦﻳا رد ﻢﺴﻴﻓر
ﺸﻧﺪ
(Sharma et al., 2011).
. ﻪﻛ ﻪﻧﻮﮕﻧﺎﻤﻫ
ﻲﻣ هﺪﻫﺎﺸﻣ زا ﻪﻛ تﺎﻌﻟﺎﻄﻣ ﻦﻳا ﺐﻠﻏا رد دﻮﺷ
ﻚﻴﻨﻜﺗ
PCR-RFLP
رد ﺖﺳا هﺪﺷ هدﺎﻔﺘﺳا
هﺪﺸﻧ هﺪﻫﺎﺸﻣ ﻲﻤﺴﻴﻓرﻮﻣ ﻲﻠﭘ ﺮﻛﺬﻟا قﻮﻓ هﺎﮕﻳﺎﺟ
ﺖﺳا .
ﮋﭘ ﻦﻳا ﺞﻳﺎﺘﻧ تﺎﻌﻟﺎﻄﻣ ﺎﺑ ﺶﻫو ﺮﮕﻳد
ﻴﻘﻘﺤﻣﻛ ﻦ نژ ﻲﻠﻜﺷﺪﻨﭼ ﻦﻴﺑ ﻪﻛ ﺪﻧدﺮﻛ شراﺰﮔ ﻪ
IGFBP3
ﻪﺷﻻ تﺎﻔﺻ و وﺎﮔ رد ﻲﻨﻌﻣ طﺎﺒﺗرا
ﺖﺷاد ﻲﻧاﻮﺨﻤﻫ دراد دﻮﺟو يراد)
Sun., 2002
.(
ﻲﻨﻌﻣ طﺎﺒﺗرا ﻦﻴﻨﭽﻤﻫ نژ ﻦﻳا ﻲﻠﻜﺷﺪﻨﭼ ﻦﻴﺑ يراد
ﻦﻳﺎﺘﺸﻠﻫ يﺎﻫوﺎﮔ رد ﻪﺷﻻ و ﺪﺷر تﺎﻔﺻ و
شراﺰﮔﺖﺳا هﺪﺷ)
Chudhary., 2007
(. رد
ﻪﻌﻟﺎﻄﻣﺮﮕﻳد يا ﻲﻨﻌﻣ طﺎﺒﺗرا ﺰﻴﻧ ﻲﻠﭘ ﻦﻴﺑ يراد
ﺎﺑ نژ ﻦﻳا ﺮﺗﻮﻣوﺮﭘ ﻪﻴﺣﺎﻧ رد دﻮﺟﻮﻣ ﻢﺴﻴﻓﺮﻣ
ﻪﺷﻻ تﺎﻔﺻ)رﻮﻜﺳا ﮓﻨﻴﻠﺑرﺎﻣ (هﺮﻛ يﺎﻫوﺎﮔ رد يا
ﺮﻛ شراﺰﮔﺪﻧد)
Cheon et al., 2008
.( ﻦﻴﻨﭽﻤﻫ
ﻲﻨﻌﻣ طﺎﺒﺗراﻲﻠﭘ ﻦﻴﺑ يراد ﺎﺑ نژ ﻦﻳا ﻢﺴﻴﻓرﻮﻣ
شراﺰﮔ كﻮﺧ رد ﺖﺸﭘ ﻲﺑﺮﭼ ﺖﻣﺎﺨﺿ ﺖﻔﺻ
ﺖﺳا هﺪﺷ
(Wang et al., 2009)
. ﺮﮕﻳد فﺮﻃ زا
رد نژ ﻦﻳا رد ﻲﻠﻜﺷﺪﻨﭼ ﭻﻴﻫﺶﻴﻣوﺎﮔ ﻦﻴﺑ يﺎﻫ
ﺸﻧ هﺪﻫﺎﺸﻣ يﺪﻨﻫﺪﺖﺳا ه)
Padma et al., 2004
(
ﺗ رد ﺎﻣ ﺞﻳﺎﺘﻧ ﺎﺑ ﺐﻠﻄﻣ ﻦﻳا ﻪﻛدﻮﺑ ﺾﻗﺎﻨ .ﺎﺠﻧآ زا
ﻪﻛنژ ﻪﺒﺷ ﺪﺷر رﻮﺘﻛﺎﻓ ﻪﺑ هﺪﻧﻮﺷ ﺪﻧﺎﺑ ﻦﻴﺌﺗوﺮﭘ
ﭗﻴﺗ ﻦﻴﻟﻮﺴﻧا3 رﻮﺤﻣ رد يﺪﻴﻠﻛ ﺶﻘﻧ
،دراد ﻚﻴﭘوﺮﺗﻮﺗﺎﻣﻮﺳ ﻪﺷﻻ تﺎﻔﺻ ﺎﺑ نآ طﺎﺒﺗرا و
،ﺖﺳا هﺪﺷ ضﺮﺤﻣ ﻲﺑﺮﭼ و ﺮﻛرﺎﻣ ﻚﻳ ناﻮﻨﻋ ﻪﺑ

دﺎﺷ ﻲﻧﺎﺧ ﻲﺿﺎﻗ ،نارﺎﻜﻤﻫ و1392
١٠٨
و ﺪﺷر تﺎﻔﺻ ﻲﺳرﺮﺑ رد ﻢﻬﻣ ﻲﻟﻮﻜﻟﻮﻣ
ﻣﺪﻴﻟﻮﺗماد رد ﻲﻠﺜﻪﻋرﺰﻣ يﺎﻫﻲﻣ ﻲﻳااﻮﺗﺪﻧ هدﺎﻔﺘﺳا
ﻮﺷد هداد ﻪﻜﻨﻳا ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ و ﻖﻴﻘﺤﺗ ﻦﻳا يﺎﻫ
ﻪﻛ ﺖﺳا ﺮﺘﻬﺑ ،ﺖﺳا يرﺎﺘﺸﻛ ﻞﻣﺎﻛ ﻲﻳﺎﺳﺎﻨﺷ ياﺮﺑ
ﺶﻬﺟ ترﻮﺻ رﺎﻛ رﻮﺘﺳد رد نژ ﻲﺑﺎﻳ ﻲﻟاﻮﺗ ﺎﻫ
ﻲﺘﺣ و دﺮﻴﮔنﺎﻜﻣﻻا هداد يورﻖﻴﻗد يﺎﻫﺮﺗ
ﻲﻫﺎﮕﺘﺴﻳا)هرود ﻪﻛ شروﺮﭘ يو ﺖﻳﺮﻳﺪﻣ ﻞﻣاﻮﻋ
هﺪﺷ ﻪﺘﺧﺎﻨﺷ ﻪﻳﺬﻐﺗ و ﺖﺳا( تاﺮﺛا ﺎﺗ دﻮﺷ مﺎﺠﻧا
ﻣﺎﻛ نژ ﻦﻳاﻞﻖﻴﻗد و ﺮﺗﺮﺗ دﻮﺷ ﻪﺘﺧﺎﻨﺷ .
ﻊﺑﺎﻨﻣ
Ali BA, EL-Hanafy AA, Salem HH (2009). Genetic biodiversity studies on IGFBP-3 gene in
Egyptian sheep breeds. Biotechnology in Animal Husbandry 25: 101-109.
Bale IK, Conover CA (1992). Regulation of insulin like growth factor binding protein 3
messenger ribonucleic acid expression by insulin like growth factor-1. Journal of
Cellular Physiology 158 : 198–204.
Cheong HS, Yoon DH, Kim LH, Park BL, Lee HW, Kim SNEM, Chung ER, Shin HD
(2008). Association Analysis between Insulin-like Growth Factor Binding Protein 3
(IGFBP3) Polymorphisms and Carcass Traits in Cattle. Journal of Asian-Australian
Journal of Animal science 21: 309-313.
Chudhary V, Kumar P, Bhattachraya TK , Bhushan B, Sharma A, Shukla A (2007). DNA
polymorphism of insulin-like growth factor-binding protein-3 gene and its association
with birth weight and body weight in cattle. Journal of Animal Breeding and Genetics 1:
29-34.
Curia RA, De HN, Oliveirab A, Silveirab C, Lopesa CR (2005). Association between IGF-I,
IGF-IR and GHRH gene polymorphisms and growth and carcass traits in beef cattle .
Livestock Production Science 94: 159–167.
Duan C, Xu Q (2005). Roles of insulin-like growth factor (IGF) binding proteins in
regulating IGF action. General and Comparative Endocrinology 142: 44-52.
Haegeman A, Van Zeveren V, Peelman LJ (1999). A new mutation in the bovine insulin-like
growth factor binding protein-3. Journal of Animal Genetics 30: 382-405.
Hastie PM, Onagbesan OM, Hareign W (2004). Co-expression of messenger ribonucleic acids
encoding IGF-I, IGF-II; type I and II IGF receptors and IGF-binding proteins (IGFBP-1
to -6) during follicular development in the ovary of seasonally anoestrous ewes. Journal
of Animal Reproduction Science 84 : 93–105.
Hossner KL, McCusker RH, Dodson MV (1997). Insulin-like growth factors and their
binding proteins in domestic animals.Journal of Animal Science 64: 1-15.
Khaldari M (2007). Husbandry of sheep and goat. Iranian Students Book Agency, INC. Iran.
11Kumar RP, Choudhary V, GaneshkumarK, Bhattacharya TK, Bhushan B, Sharma A,
MishraA (2006). Nucleotide sequencing and DNA polymorphism studies on IGFBP-3
gene in sheep and its
comparison with cattle and buffalo. Small Ruminant Research 64:
285 –292.
Maciulla JH, Zhang HM, Denise SK (1997). A novel polymorphism in the bovine insulin-like
growth factor binding protein-3 (IGFBP-3) gene. Journal of Animal Genetics 28: 375.
Martin JL, Baxter RC (1992). Insulin-like growth factor binding protein-3, biochemistry and
physiology. Growth Regulat 2: 88-89.
Miller SA, Dykes DD, Polesky HF (1988). A simple salting out procedure for extracting
DNA from human nucleated cells. Nucleic Acids Research 16: 1215.

يزروﺎﺸﻛ يژﻮﻟﻮﻨﻜﺗﻮﻴﺑ ﻪﻠﺠﻣ) هرود5 هرﺎﻤﺷ ،4 ،نﺎﺘﺴﻣز 1392(
١٠٩
Moradi shahrebabak H, Moradi shahrebabak M, Mehrabani yeganeh H (2009). Association
polymorphism of the genes calpastatin, miostatin, leptin and potassium with economic
traits, blood metabolite and carcass traits in makoei and zel sheep. Thesis of Ph.D.
University of Tehran (In farsi).
Morimoto LM, Newcomb PA, White E, Bigler J, Potter JD (2005). Variation in Plasma
Insulin-Like Growth Factor-1 and Insulin-Like Growth Factor Binding Protein-3:
Genetic Factors. Journal of Cancer Epidemiol Biomarkers Prevention 14: 1394-1401.
Padma B, Kumar P, Choudhary V, Dhara SK, Mishra A, Bhattacharya TK, Bhushan B,
Sharma A (2004). Nucleotide sequencing and PCR-RFLP of insulin-like growth factor
binding protein-3gene in riverine buffalo (Bubalus bubalis). Asian-Australasian Journal
of Animal Sciences 17: 910-913.
Sharma B, Dass G, Sharma D (2011). PCR-RFLP in IGFBP-3 Gene and its Association with
Economic Traits in Muzaffarnagari Sheep. International Conference on Chemical,
Biological and Environment Sciences. Bangkok. Thailand.
Shukla A (2001). PCR-RFLP studies on insulin-like growth factor binding protein 3 (IGFBP-
3) gene in cattle. M.V.Sc. Thesis submitted to the Indian Veterinary Research Institute
(Deemed University), Izatnagar, Bareilly, UP, India.
Spratt SK, Tatsuno GP, Sommer A (1991). Cloning and characterization of bovine insulin
like growth factor binding protein- 3 (IGFBP-3) gene. Biochemistry Biophysics
Researches Communication 177: 1025-1032.
Sun WB (2002). Polymorphism of insulin like growth factor binding protein-3 (IGFBP-3)
gene and its relation with beef performance of Qinchuan cattle. Journal of Animal
Biotechnology 8: 95-99.
Wang W, Meng Q, Hu X, Li N (2009). Genetic Variation and Association of Insulin-Like
Growth Factor Binding Protein-3 with Performance in Swine. Journal of Biochemical
Genetics 47: 315-321.

دﺎﺷ ﻲﻧﺎﺧ ﻲﺿﺎﻗ ،نارﺎﻜﻤﻫ و1392
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Study polymorphism of ovine IGFBP-3 Gene and its association with carcass traits and
fatty acids profiles in Zel sheep using PCR-SSCP
Ghazi Khani Shad.A
∗
∗∗
∗1
., Moradi-Shahrbabak H
2
., Sadeghi M
2
., Faraji R
3
., Mohammadabadi
M.R.
4
1
Department of Animal Science, Saveh Branch, Islamic Azad University, Saveh, Iran
2
Assistant Professor, Department of Animal Science, University of Tehran, Karaj, Iran.
3
M.Sc. student, Department of Animal Science, University of Tehran, Karaj, Iran.
4
Associate Professor, Department of Animal Science, Shahid Bahonar University of Kerman, Kerman,
Iran.
Abstract
IGF-1 hormone
transports by connecting to IGF-binding proteins (often IGFBP-3) in
the biological fluids. This protein acts as an IGF1 carrier and helps to prolong half-life of
hormone and can increase or decrease biological activity of hormone on cellular surface. The
purpose of the research was to study the polymorphisms of IGFBP-3 gene and its association
with carcass traits and fatty acids profiles in Zel sheep as a thin-tailed breed using PCR-SSCP.
In this study we used 130 Zel sheep randomly and recorded their traits measure we wanted. to
determine of fatty acids, we took 6ml blood in venojects containing EDTA and immediately
centrifuged them for 10 minutes by 3000g (5200 rpm) to separate the plasma. DNA was
extracted from muscle tissue by Biotech company kit. Then the Polymerase chain reaction
(PCR) used to amplify of 654 bp fragment of exon 3 of IGFBP-3 gene. PCR products were
electrophoresed on polyacrylamide gel (method SSCP) and stained with silver nitrate method
to distinguish different patterns. Results indicated the different patterns, may be due to
polymorphism in this fragment. Frequency of patterns 1, 2, 3, 4 and 5 were 67/66, 33/3, 67/6,
33/13 and 10 percent respectively. Trait carcass weight (P <0.05), back fat thickness (P <0.01)
and crude protein in meat (P <0.01) had significantly differences with different patterns.
Significant association observed by Heptadecanoic acid (C17: 0) with different patterns of
IGFBP3 gene. No other association was observed by IGFBP3 patterns with other traits and
fatty acids profiles.
Key words: Ovine IGFBP-3 gene, PCR-SSCP, Fatty acids profiles, Zel.
∗
Corresponding Author: Ghazi Khani Shad.A Tel: 09121466570 Email: A.ghazikhani@iau-saveh.ac.ir