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Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-bisphosphate carboxylase

Authors:
Guido Van den Broeck
Guido Van den Broeck
Guido Van den Broeck
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Michael Timko at University of Virginia
Michael Timko
Albert Kausch at University of Rhode Island
Albert Kausch
Anthony R. Cashmore
Anthony R. Cashmore
Anthony R. Cashmore
  • This person is not on ResearchGate, or hasn't claimed this research yet.
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Abstract

Chimaeric genes can be constructed which fuse the transit peptide of a small subunit of the chloroplast-located ribulose 1,5-bisphosphate carboxylase with a bacterial protein. The fusion protein is translocated into chloroplasts and cleaved in a similar way to the small subunit polypeptide precursor.
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© Nature Publishing Group1985
© Nature Publishing Group1985
© Nature Publishing Group1985
© Nature Publishing Group1985
© Nature Publishing Group1985
© Nature Publishing Group1985
... However, other genes have been used for fusions with great success. These include the galK (galactokinase [73]), the cat (chloramphenical transacetylase [47]), the npt (neomycin phosphotransferase [117]), and the phoA (alkaline phosphatase [57a; C. Manoil and J. R. Beckwith, Proc. Natl. ...
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