Yasemin G Isgor

Ankara University · Vocational School of Heath Services

Background Dye-exclusion based cell viability analysis has been broadly used in cell biology including anticancer drug discovery studies. Viability analysis refers to the whole decision making process for the distinction of dead cells from live ones. Basically, cell culture samples are dyed with a special stain called trypan blue, so that the dead...

Background and Objective: The discovery of traditional plants with some medicinal properties, verifying their biological targets and the bioassay guided standardization of their active components are the particular interest of diverse research groups recently. These efforts may help to revise the therapy modalities with natural product supplements....

Counting of cells can give useful information about the cell density to understand the concerning cell culture condition. Usually, cell counting can be achieved manually with the help of the microscope and hemocytometer by the domain experts. The main drawback of the manual counting procedure is that the reliability highly depends on the experience...

Plants and most of the plant derived compounds have been known because of their potential pharmaceutical effects for a long time. They are playing an important role on the treatment of several diseases from diabetes to various types of cancers. Today most of the clinically effective pharmaceuticals are developed from plant derived ancestors in the...

Eryngium campestre L. (Apiaceae) are known in Turkish folk medicine as ‘Bogadikeni’ and wildly distributed in all parts of Turkey. Infusion of different parts of this species are well known as diuretic, appetizer and stimulant. In the present study, methanol extracts from flowers and leaves of E. campestre were evaluated for their potential medicin...

Context: Plants and most of the plant-derived compounds have long been known for their potential pharmaceutical effects. They are well known to play an important role in the treatment of several diseases from diabetes to various types of cancers. Today most of the clinically effective pharmaceuticals are developed from plant-derived ancestors in t...

The metal coordination complexes are known to induce cytotoxic effects on various cell lines and shown to have great potential for therapeutic interventions. Their main mechanism of action is through the mediation of enzyme activities in signaling pathways essential for cellular functioning. The overall cellular responses are dose-dependent and req...

The aim of this study is to design and synthesize novel dual inhibitors of Src protein tyrosine kinase (PTK) and Glutathione S-transferases (GSTs), as a potential drug lead with therapeutic efficacy on cancer and immune disorders. The biological activity profiling of small molecule inhibitors via miniaturized biochemical techniques compatible with...

The increased activity levels of Glutathione S-transferases (GST) have been correlated with human cancers and the anticancer drug resistance. Similarly, Src family kinases (SFKs), has been reported in many cancers including breast, colon, lung, and skin with relatively high catalytic activity. Therefore, the inhibition of both GSTs and Src may enha...

Perylene derivatives, known to have potential therapeutic benefits on particular cancer types as photosensitizers, may also function as small-molecule inhibitors with promising therapeutic value for diverse diseases. This recently recognized biological activity was attributed to their capacity to modulate the function of various enzymes as biologic...

Doxazosin is one of the quinazoline-based alpha 1-adrenergic receptor antagonists in clinical use for the treatment of hypertension and benign prostate hyperplasia. Doxazosin-induced cytotoxicity studies, resulted in growth inhibition and apoptosis, show its potential therapeutic benefits for several forms of cancers. These effects on cells occur a...

Kinases, representing almost 500 proteins in the human genome, are responsible for catalyzing the phosphorylation reaction of amino acid residues at their targets. As the largest family of kinases, the protein tyrosine kinases (PTKs) have roles in controlling the essential cellular activities, and their deregulation is generally related to patholog...

Oxidative stress has been implicated in aging and in a variety of diseases affecting the nervous, respiratory, cardiovascular and gastrointestinal system in humans. Reactive oxygen species (ROS) have been associated with mechanisms to activate kinases, such as protein tyrosine kinases, which may initiate malignant transformation. Significant eviden...

Current evidences demonstrated that the activity of protein kinases can be controlled through oxidative stress induced by reactive oxygen species (ROS) and normalized by antioxidants. Recent studies with ROS, generated by mitochondria, suggested the potential signalling role of these species, where ROS, especially hydrogen peroxide, were proposed a...

A series of N-benzyl-indole-3-imine-, amine derivatives and their 5-bromo congeners were synthesized and their biological activity were evaluated against the pp60(c-Src) tyrosine kinase target. To afford the imine derivatives, aldehydes were reacted with substituted benzylamines and the corresponding amine derivatives were obtained by NaBH(4) reduc...

A series of novel 1,3,5-trisubstituted indole derivatives, namely, N-benzyl 5-phenyl indole-3-imine, N-benzyl-5-(p-fluorophenyl)indole-3-imine and their corresponding amine congeners, were designed and synthesized as pp60(c-Src) tyrosine kinase inhibitors, and their inhibitory activities toward pp60(c-Src) tyrosine kinase were evaluated by in-vitro...

The pp60(c-Src) is one of the ubiquitously expressed Src family kinases and has important functions in malignant cells, including regulation of cell division, growth factor signaling, and movement. Therefore, investigating new small molecule inhibitors of pp60(c-Src) is important to discover and develop novel therapeutics for cancer and metastasis....

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Therapy with receptor tyrosine kinase inhibitors provides an improved treatment option in a number of diseases such as cancer, myocardial infection, osteoporosis, stroke, and neurodegeneration. We have designed, synthesized, and evaluated a series of novel 2-amino-5-[(benzyl)imino]methyl-3,7-dihydro-4H-pyrrolo[2,3-d]pyrimidine-4-one 7a and 2-amino-...

Purpose: Studies with garlic have shown the medicinal value of the plant by its antioxidant potential which is exerted by its organosulfur rich content. In this study, the aim is to determine any potential antioxidant efficacy of the less toxic water soluble extract of a widely cultivated garlic form in Turkey, in terms of alterations on the oxidan...

Combinatorial chemistry has become an important tool for design and discovery of novel chemical molecules. This technique allows for the production of large numbers of different molecules simultaneously. Pharmaceutical industries use combinatorial chemistry to create libraries of organic molecules for drug screening and lead optimisation. This revi...

Mitochondria are principal mediators of apoptosis and thus can be considered molecular targets for new chemotherapeutic agents in the treatment of cancer. Inhibitors of mitochondrial complex I of the electron transport chain have been shown to induce apoptosis and exhibit antitumor activity. In an effort to find novel complex I inhibitors which exh...

We have synthesized and spectrally characterized a squaraine-based fluorescent chemosensor for pH. This chemosensor unlike many others, works in 100 % aqueous solutions, signalling the pH change from 10 to 7 by a 14-fold increase in the emission intensity at 651 nm, when excited at the isosbestic point (614 nm). The average pKa for the ionizable gr...