Nancy Santana-Buzzy

Centro de Investigación Científica de Yucatán | CICY · Biochemical and Molecular Biology Unit

The objective of this work was to obtain high‐yielding F 1 hybrids of the habanero pepper, using 10 outstanding parents in a line‐tester genetic design. General combinatorial ability and specific combinatorial ability were evaluated in the parents' and the hybrids F 1 obtained, respectively. Heterosis was determined in the 18 hybrids obtained. The...

Selection of lines from segregating generations and breeding hybrids to exploit heterosis or heterobeltiosis is an effective tool for improving economically important traits in the genus Capsicum . The objective of this study was to identify high potential parents to obtain high-yielding F 1 hybrids using a Line × Tester (8 × 2) mating design in th...

The objective of this study was to establish an efficient—direct or indirect—regeneration system for pineapple (Ananas comosus L.) plants, with a high rate of multiplication and that would preserve the genetic identity of the donor genotype (Hybrid ‘MD2’) in the regenerated plants. Ten treatments, with different concentrations of 2,4-Dichlorophenox...

Zygotic and somatic embryogenesis in plants is a fascinating event that is finely regulated through the expression of a specific group of genes and dynamic levels of plant hormones whose concerted action determines the fate that specific cells follow towards zygotic or somatic embryo development. This work studied different stages of Capsicum chine...

This study evaluated 29 F1 lines and the 11 genotypes of habanero peppers used in the crossbreeding program developed by the Scientific Research Center of Yucatan, México. A randomized complete block design with four repetitions was used. Eight plants of each of the genotypes were studied per block. A total of 22 qualitative and 18 quantitative des...

La quitinasa y B -1,3-glucanasa desempeñan una función importante en la reacción de defensa contra patógenos fúngicos. En Agave americana existe poca información sobre el comportamiento de estas proteínas en respuesta a la infección de Fusarium oxysporum . En este estudio, se realizó un análisis de microscopía electrónica de barrido (MEB) en raíces...

Knowledge of the reproductive biology of a species is fundamental in order to develop an efficient program of genetic improvement by hybridization. The viability of the pollen, anther dehiscence, receptivity of the stigma and the anthesis of 12 improved lines of Habanero pepper were studied to develop a cross-breeding program. Among the results, th...

Somatic embryos (SE) of habanero pepper (Capsicum chinense Jacq.) represent persistent deformations in the shoot apical meristem (SAM), which inhibits their capacity to form organs and subsequently plants. In dicotyledonous plants, SAM is formed in the apex, between cotyledons and it plays a central role in postembryonic shoot organ formation. Base...

The Yucatan Peninsula is recognized as the center of genetic diversity of Habanero peppers (Capsicum chinense Jacq.), which can be distinguished from those cultivated in other regions of the world by their aroma, taste, and—most of all—by their pungency. We evaluated three commercial varieties of chili peppers reported as being the hottest in the w...

The variability and genetic parameters of seven agronomic characteristics were estimated for 11 genotypes, and high values of the phenotypic coefficient of variation (PCV) of capsaicin content (CC) were obtained. Heritability (h ² ) was high for yield per plant (YP; 0.98) and CC (0.93). The principal components analysis (PCA) revealed that the firs...

The behavior of endogenous polyamines was studied in somatic embryos and zygotic embryos of Habanero pepper (Capsicum chinense). In the first part of the work, the polyamine content was evaluated in both types of embryos (somatic and zygotic). As a result, in addition to the common polyamines (putrescine, spermidine and spermine), it was also possi...

In vitro screening for osmotic tolerance was developed during somatic embryogenesis in Habanero pepper (Capsicum chinense Jacq.). Different concentrations of high molecular weight polyethylene glycol (PEG-8000) were used as selective agent to induce water stress in Murashige and Skoog liquid medium. The lethal dose (LD50) and content of free prolin...

Intersimple sequence repeat (ISSR) markers were used to evaluate the effects of in vitro culture on genetic variation in Habanero pepper (Capsicum chinense Jacq.) regeneration protocols. A total of 219 ISSR clear and reproducible fragments were generated with 13 ISSR primers in direct organogenesis, direct and indirect somatic embryos, and the embr...

Somatic embryo-like structures were produced from the hypocotyls of aseptic plants of Capsicum chinense. Different concentrations of 2,4-dichlorophenoxyacetic acid (0, 4.5, 9.05 μM), several exposure times of the explant to this auxin (15, 30, 45, 60 days) and the development of somatic embryos cultured in a solid and/or liquid medium were evaluate...

Jatropha curcas is a small bush which has attracted attention as an energy crop for biodiesel production. A number of reports have been published regarding the toxic J. curcas but there is little information relating to non-toxic J. curcas. A simple, high-frequency protocol for plant regeneration through organogenesis and somatic embryogenesis has...

Most of the cycads contain high concentrations of essential oils, flavonoids, polyphenols, and polysaccharides that interfere with DNA extraction, causing erroneous or no PCR products. The optimization of DNA isolation, employing inter-simple sequence repeats (ISSRs) primers were investigated in Ceratozamia mexicana Brongn., an endangered Mexican c...

This book (19 chapters) on pepper ( Capsicum spp.) provides an overview of current research on ethnobotany, taxonomy, genetics, greenhouse production, tissue culture, transplant production, transplanters used in pepper production, stand establishment, fertilizer application and irrigation management, cropping systems, salt tolerance, organic produc...

The effect of NaCI salinity on growth and development of somatic embryos of Habanero pepper was examined. Addition of 75 and 100 mM NaCI into the medium greatly increased the growth and development of somatic embryos and both of these concentrations favored the proliferation of somatic embryos. However, supplementation of 200 and 300 mM NaCI to the...

This article describes the performance of nodal segments from Habanero pepper (Capsicum. chinense) during shoot induction and elongation under different semisolid and liquid culture conditions with various degrees of ventilation in which they were exposed to different levels of immersion and growth regulators. The ethylene content in non-ventilated...

Somatic embryoids obtained from germinated seeds in an MS culture media supplemented with naphthalene acetic acid (NAA), 6, bencylaminopurine (BAP) and indole-3-acetic acid (IAA), 2 mg·liter-1 each, were encapsulated in sodium alginate in complex with calcium chloride (CaCl 2·2H2O) 75 mM matrix, to produce synthetic seeds, as a rescue and conservat...

We analysed changes in the transcript population produced in habanero pepper (Capsicum chinense) cell suspensions by the addition of whole mycelium homogenates from a pathogenic isolate of Phytophthora capsici, to identify plant cellular processes modified by the oomycete effectors. The elicitation produced several defence-like cellular responses:...

The ontogenesis of direct high-frequency somatic embryogenesis of C. chinense induced from hypocotyl was characterized through a histological analysis of the different phases in the histodifferentiation process during the development of the somatic embryo. The anatomical analysis was carried out since the hypocotyl segments were placed in the cultu...

Somatic embryoids obtained from germinated seeds in an MS culture media supplemented with naphthalene acetic acid (NAA), 6, bencylaminopurine (BAP) and indole–3–acetic acid (IAA), 2 mg·liter–1 each, were encapsulated in sodium alginate in complex with calcium chloride (CaCl2·2H2O) 75 mM matrix, to produce synthetic seeds, as a rescue and conservati...

The aim of this study was to determine the pungency level of different accessions of Habanero peppers. The high-performance liquid chromatography (HPLC) technique was used to evaluate the content of total capsaicinoids in the whole fruit, placenta, and pericarp of 18 accessions of Habanero pepper from the germplasm bank of the Capsicum chinense spe...

A complete and efficient regeneration protocol was developed for Vanilla planifolia 'Andrews', an endangered orchid species that represents an important crop in several tropical countries. Axillary buds excised from the first to the eighth node, considering the first to fourth nodes as "young" (zone 1) and the fifth to eighth as "mature" (zone 2),...

A complete and efficient regeneration protocol was developed for Vanilla planifolia ‘Andrews’, an endangered orchid species that represents an important crop in several tropical countries. Axillary buds excised from the first to the eighth node, considering the first to fourth nodes as “young” (zone 1) and the fifth to eighth as “mature” (zone 2),...

Genetic improvement of coffee, an important commercial crop, through classical breeding is slow and cumbersome. Biotechnology offers alternative strategies for generating new and improved coffee varieties, including those resistances to environmental extremes, pests, and diseases, low in caffeine, and with uniform fruit maturation. Large improvemen...

To determine the effect of different nitrogen sources and osmotic regulators on minimal growth of Habanero pepper (Capsicum chinense Jacq.) germplasm for in vitro conservation, different concentrations of nitrate, sucrose, mannitol, and sorbitol were evaluated. The micropropagation system based on Santana-Buzzy et al. (2006) culture medium was modi...

To induce the somatic embryogenesis of Habancro pepper, different culture media and different types of explants (node, internode, hypocotyl, half seeds, and fruit segments) were evaluated. For the induction of embryogenic callus, 9.05 mu M of 2,4-dichlorofenoxiacetic acid, 3% sucrose, and 0.8% gelrite were added to the basic MS medium over a period...

To induce somatic embryogenesis in habanero pepper (Capsicum chinense Jacq.), the cultivar BVII-03, belonging to the red type, was used. Different explants were evaluated, as were different culture media, the composition of which varied in the content of plant growth regulators. Results showed the formation of somatic embryos from cotyledons, zygot...

The in vitro production of ethylene and its effects on the development of Habanero pepper (Capsicum chinense Jacq.) plantlets were evaluated using nonventilated containers (NVCs) and ventilated containers (VCs). Shoots of Habanero pepper between 0.5 and 1.0 cm of height were cultivated in Magenta culture boxes and samples of the headspace atmospher...

To induce multiple shoots from habanero pepper (Capsicum chinense Jacq.), nodes and stem segments were cultivated in MS medium supplemented with varying concentra-tions of kinetin, benzyladenine, and thidiazuron. The effect of the age of the explant in the medium on shoot formation and their latter development into plants was assessed. Ethyl-ene co...

Mature seeds of Coffea arabica L. cvs Caturra and Catimor stored for 0-18 months were evaluated for germination and growth in a greenhouse. Zygotic embryos were isolated, germinated in vitro and after germination grown in a greenhouse. We observed high in vitro germination (75-100%) with time versus a sharp decrease in germination percentage (100-2...

Protein profile was studied during the development of Capsicum chinense somatic embryos. The total protein content and profile of polypeptides (by sodium dodecyl sulfate polyacrylamide gel electrophoresis) of somatic embryos at different developmental stages (globular, heart-shaped, torpedo and cotyledonary stages) were analyzed. The protein profil...