Miguel Angel Ramos-Lopez

Autonomous University of Queretaro · Facultad de Química

The competitive colonization of bacteria on similar ecological niches has a significant impact during their establishment. The synthesis speeds of different chemical classes of molecules during early competitive colonization can reduce the number of competitors through metabolic effects. In this work, we demonstrate for the first time that Kosakoni...

El biodiésel obtenido a partir de aceite de diferentes fuentes de biomasa es una alternativa a la reducción del uso de combustibles fósiles para el transporte, y por esto se han estudiado las larvas de la mosca soldado negro Hermetia illucens, en las cuales se puede aumentar su contenido de aceite por medio de diversos factores en esta fase de su d...

Spodoptera frugiperda is the main pest of maize. One of the alternatives proposed for its control is the implementation of products of botanical origin, such as those derived from Ricinus communis. In this work, the insecticidal and insectistatic activities of methanolic and hydrolyzed methanolic extracts of the aerial parts of R. communis and kaem...

Electrostatic fermentation avoids the cellular redox imbalance of traditional fermentation, but knowledge gaps exist. This study explores the impact of electrostatic fermentation on the growth, volatile profile, and genetic response of Saccharomyces pastorianus Saflager S-23. The applied voltage (15 and 30 V) in the electrostatic fermentation syste...

Chili powder is an important condiment around the world. However, according to various reports, the presence of pathogenic microorganisms could present a public health risk factor during its consumption. Therefore, microbiological quality assessment is required to understand key microbial functional traits, such as antibiotic resistance genes (ARGs...

The microbiome of Spodoptera frugiperda plays a fundamental role during its biological cycle, since a great diversity of microorganisms interact through different mechanisms to maintain homeostasis. Their functions include, for example, defense against pathogens, degradation and the supply of functional molecules, among others. However, factors suc...

Resumen La Higuerilla (Ricinus communis) es una planta que tiene diversas aplicaciones en biocombustibles, como son la producción de biodiésel, pellets y etanol; sin embargo, existen pocos estudios con esta planta para la producción de biogás. Este proyecto analizó el bagazo de la semilla de la higuerilla para la producción de biogás. Se analizaron...

Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae), also known as the fall armyworm, is an economically important and widespread polyphagous pest. Microorganisms associated to this insect during life cycle play important ecological roles. We report 3 metagenome-assembled bacterial genomes reconstructed from a metagenome dataset obtained from S....

For the management of Spodoptera frugiperda, botanical extracts have been used to reduce the environmental impacts of synthetic chemical pesticides. In the present investigation, the insecticidal activity of the acetonic and methanolic extracts of Heterotheca inuloides (Asteraceae) and of the main compound 7-hydroxy-3,4-dihydrocadalene on this pest...

The Kosakonia cowanii Cp1 strain was isolated from seeds of Capsicum pubescens R. & P. cultivated in Michoacan, Mexico. Genetic and ecological role analyses were conducted for better characterization. The results show that genome has a length of 4.7 Mbp with 56.22% G + C and an IncF plasmid of 128 Kbp with 52.51% G + C. Furthermore, pathogenicity t...

The Kosakonia cowanii Cp1 strain was isolated from seeds of Capsicum pubescens R. & P. cultivated in Michoacan, Mexico. Genetic and ecological role analyses were conducted for better characterization. The results show that genome has a length of 4.7 Mbp with 56.22% G + C and an IncF plasmid of 128 Kbp with 52.51% G + C. Furthermore, pathogenicity t...

The use of botanical extracts of the plant Jatropha curcas (Euphorbiaceae) represents a valuable alternative to control insect pests and avoid the detrimental effects on the environment and health that arise due to synthetic chemical insecticides. Thus, we conducted a systematic review to summarize the published evidence on the bioactivity of J. cu...

Kosakonia cowanii strain Ch1 was isolated from Mexican chili powder, and the genome was sequenced. The genome was 4,765,544 bp in length, with an average G + C content of 56.22%, and a plasmid (pCh1) of 128,063 bp with an average G + C content of 52.50%. A phylogenetic analysis revealed a close relation with pathogenic strains; nevertheless, some v...

The use of botanical extracts of the plant Jatropha curcas (Euphorbiaceae) represents a valuable alternative to control insect pests and avoid the detrimental effects on the environment and health that arise due to synthetic chemical insecticides. Thus, we conducted a systematic review to summarize the published evidence on the bioactivity of J. cu...

El tratamiento biológico es el más utilizado para las aguas residuales domésticas. El reactor de lecho fijo es una biopelícula de microorganismos capaces de degradar la materia orgánica, y es una alternativa eficiente en comparación con los sistemas convencionales, ya que aumenta la superficie de contacto entre el microorganismo y la materia orgáni...

Lignocellulosic biomass is a source of carbohydrates that can be used in the production of biogas. The aim of this study was to obtain biogas from biomass waste (leaves, stems and seed bagasse) of Ricinus communis, applying pretreatments such as temperature and humidity. We examined the effect of these pretreatments on the biomass, two enzymatic pr...

This systematic review presents the current state of research in the last five years on contaminants in soils, especially in leachates from solid waste landfills, with emphasis on biological remediation. In this work, the pollutants that can be treated by microorganisms and the results obtained worldwide were studied. All the data obtained were com...

Citation: Sandoval-Cárdenas, D.I.; Pool, H.; Favela-Camacho, S.E.; Santos-Cruz, J.; Campos-Guillén, J.; Ramos-López, M.A.; Rodríguez-deLeón, E.; Urbina-Arroyo, J.V.; Amaro-Reyes, A. Sargassum@magnetite Composite EDTA-Functionalized for the Potential Removal of Mercury. Polymers 2023, 15, 1405. https:// Abstract: Sargassum spp. affects the Caribbean...

The genus of Trichilia (Meliaceae) is widely distributed in Mexico and is a sustainable source of bioactive compounds. Trichilia havanensis Jacq. presents toxicity to insect pest larvae of the Noctuidae family. Its powdered dried fruit has shown insecticidal activity at 15 days (10,000 mg/L, 46%) against larvae of Spodoptera littoralis Boisduval (N...

Ricinus communis is a species distributed worldwide. Its seeds are used to produce castor oil, which can be used for the production of biofuels; yield improvement can be achieved with elicitors that are substances of biological origin that can induce increased productivity of primary and secondary metabolism, when applied to plants. Salicylic acid...

Chili powder is the most frequently consumed spice in Mexican diets. Thus, the dissemination of microorganisms associated with chili powder derived from Capsicum annuum L. is significant during microbial quality analysis, with special attention on detection of potential pathogens. The results presented here describe the initial characterization of...

A study of the phenomenon of sedimentation of sludge from the anaerobic digestion of codigestion phases and monostage was performed, compared with addition and without addition of coagulant, the dose and concentration required was determined in the laboratory by jar test, the concentration of FeCl3 resulted in 15000 mg / l. The results of the sedim...

A comparison was made between a tangential flow system with mixed oxide ceramic membranes and a tangential flow system with mixed oxide ceramic membranes impregnated with porcine gelatin for emerging contaminants such as tetracycline. For the impregnation of the ceramic membranes, a tangential flow system and a 1% porcine gelatin solution were impr...

In developing countries, the RAFA (Anaerobic Upflow Reactor) reactor stands out as a viable alternative in wastewater treatment. In addition, biofilters are used in the biological reactors that have the objective of biofilm formation, by means of which effluents of better quality are obtained. An anaerobic reactor of 140 Liters was evaluated for th...

Castor bean (Ricinus communis L.) is an oleaginous plant of economic importance due to its different industrial applications. Edaphic characteristics are used to determine the viability of places considered as suitable to obtain castor oil. The objectives of this study were to determine the edaphic characteristics and oil content of R. communis, an...

The species Opuntia ficus-indica is affected by pests and diseases, one of the most important of these being golden spot. The latter has been reported in various countries, including Mexico. Symptoms similar to those of golden spot in O. ficus-indica have been observed in the xoconostle crop ( Opuntia matudae Scheinvar, cv. Rosa). The objective of...

Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae) is one of the most damaging pests in maize crops. In order to manage it, synthetic insecticides such as diamides, neonicotinoids, and pyrethroids are used, but they present a risk for humans and the environment. Investigations of safer alternatives include the use of natural extracts. Thus, thi...

Actividad de pretratamientos y enzimáticos químicos en residuos de higuerilla (Ricinus communis) para la producción de biogás Colaboración Fecha de recepción: 25 de noviembre del 2021 Fecha de aceptación: 13 de diciembre del 2021 RESUMEN: Ricinus communis es una planta que tiene diversas aplicaciones en biocombustibles, tal como pro-ducción de biod...

The potential presence of spore-forming bacteria related to the Bacillus cereus group in Mexican chili powder elaborated from Capsicum annuum L. is of commercial and clinical interest, because chili powder is an essential spice in the Mexican diet and in diets around the globe. To facilitate detection and isolation of members of this group of spore...

Ulomoides dermestoides are used as a broad-spectrum medical insect in the alternative treatment of various diseases. Preliminary volatilome studies carried out to date have shown, as the main components, methyl-1,4-benzoquinone, ethyl-1,4-benzoquinone, 1-tridecene, 1-pentadecene, and limonene. This work focused on the production of metabolites and...

Antifungal composite edible coatings (ECs) formulated with pregelatinized potato starch (PPS, 1.0–2.0% w/w) as biopolymer, glyceryl monostearate (GMS, 0.5–1.5%, w/w) as hydrophobe, glycerol (Gly, 0.5–1.5%, w/w) as plasticizer, and sodium benzoate (SB, 2%, w/w) as antifungal agent were optimized using the Box–Behnken response surface methodology to...

The use of waste in the manufacture of high-value products through biorefinery could lead to a circular bioeconomy. The objective of this work was to create combined cross-linked enzyme aggregates as an added-value product, the enzymes were produced through solid-state fermentation of paperboard residue for two days. The Trichoderma reesei crude ex...

Castor bean (Ricinus communis) seeds contain ricinine, an alkaloid with insecticidal and insectistatic activities. Elicitation with salicylic acid (SA) has proven to stress R. communis and might modify the ricinine concentration. The aim of this study was to evaluate the concentration of ricinine in the bagasse of seeds from R. communis elicited wi...

There is still an incomplete defined number of the members of the genus Crataegus (hawthorn) (Rosaceae). Phipps and co-workers estimated between 150 and 200, Tassel and collaborators, 300, while others provide different numbers of species. Most of these plants are employed worldwide in traditional, and now as adjuvants in modern medicines, for the...

Fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), infests more than 80 crop species and is the principal pest of maize (Zea mays L. (Poaceae) in Mexico. Although insecticides are used to control the pest, they can cause environmental damage and negatively affect human health. Essential oils are an alternate for managing th...

The main objective of the study was to identify chemical compounds of extract of acetone of shell + almond (EACA) and nut of almond (EAA) of Jatrofa curcas and evaluate bioactivity against Copitarsia decolora in laboratory and greenhouse conditions. In ingestion experiments, extracts at 250, 500, 1,000, 1,500, and 2,000 ppm were mixed into artifici...

Weed control in sustainable agriculture requires new bioherbicidal molecules to replace synthetic herbicides that have damaged the environment and generated resistance in weeds. This study was conducted to investigate the chemical composition of Eucalyptus globulus essential oil and to explore its bioherbicidal potential on the germination and radi...

The popping process has been widely used as a technique for obtaining snacks. This study evaluated the effect of the popping process on the structural and thermal properties of sorghum. Seven varieties of sorghum were used. Raw sorghum grains were adjusted to 11% moisture and popped at 210°C for 90 s with hot air. Microstructure, thermal and viscos...

Antifungal composite edible coatings (ECs) formulated with pregelatinized potato starch (PPS, 1.0-2.0 % w/w) as biopolymer, glyceryl monostearate (GMS, 0.5-1.5 %, w/w) as hydrophobe, glycerol (Gly, 0.5-1.5 %, w/w) as plasticizer, and sodium benzoate (SB, 2 % w/w) as antifungal agent were optimized using the Box–Behnken response surface methodology...

Some botanical extracts affect the cellular immune system of insects by interfering with normal hemocyte function. This gives rise to diminution in cellular immunity and causes a decrease in insect defense. This investigation evaluated the effect of acetonic (AcEDv) and methanolic (MeEDv) extracts of Dodonaea viscosa (1000, 2000, and 4000 ppm) on t...

A pre-adaptation approach to osmotic stress was used to improve the colonisation and biocontrol capacity of Yarrowia lipolytica M18/3b on the apple under pre- and postharvest conditions. For this, M18/3b strain was cultured in an unmodified NYDB medium (0.996a w) or in different media with water stress (0.950–0.970a w) produced by the addition of N...

Bee pollen is a favorable microhabitat for many microorganisms and its dissemination via commercialization must be of important consideration. Here the data presented describe the characterization of bacteria and fungi community structure in seven commercial bee pollen samples from Europe, Mexico, and Chile, obtained from a commercial supplier, whi...

The treatment of bacterial diseases in aquaculture is done using antibiotics, their applications has resulted in contamination and bacterial resistance. Natural extracts are a potential alternative as an antimicrobial, they have demonstrated effectiveness in their use aimed at treating conditions. The purpose of this study was to evaluate the antim...

Sustainable processing from native vegetation such as agave, nopal and aloe, is mainly centered on the components of lignin, cellulose, and hemicellulose, defined generally as lignocellulosic. Cytotoxicity of lignocellulosic biopolymer membranes (LBMs) of mucilages of Opuntia ficus-indica, Aloe barbadensis Miller, fructans, and residual Agave tequi...

In Mexico little is known about high-altitude glacial psychrotolerant or psychrophilic fungal species, with most glacial fungi isolated from polar environments or Alpine glaciers. It has been documented that some of these species may play an important role in bioremediation of contaminated environments with heavy metals. In the present study, 75 fu...

Background: In spore-forming bacteria, the molecular mechanisms of accumulation of transfer RNA (tRNA) during sporulation must be a priority as tRNAs play an essential role in protein synthesis during spore germination and outgrowth. However, tRNA processing has not been extensively studied in these conditions, and knowledge of these mechanisms is...

Endospore-forming bacteria related to the Bacillus cereus group produce toxins that cause illnesses in organisms from invertebrates to mammals, including foodborne illnesses in humans. As commercial bee pollen can be contaminated with these bacteria, a comprehensive microbiological risk assessment of commercial bee pollen must be incorporated into...

Spodoptera frugiperda J. E. Smith (Lepidoptera: Noctuidae) (fall armyworm) is a pest native to the Americas that affects a variety of crops. Its control is based on chemical insecticides. However, this practice has been associated with changes in the susceptibility of pests to various insecticides. The use of plant products represents an eco-friend...

Bee pollen is a valuable natural product and by its nutritional composition is important for the development of insects of agricultural interest. In this work, we determined whether gamma irradiation can minimize the risk of microbial contamination of commercial bee pollen used for the mass rearing of bumblebees. Bee pollen samples were treated wit...

It has been proposed systems that adapt economically and technically to the conditions of most places that have a considerable water consumption. The work consisted of an anaerobic biological reactor in which the treatment of domestic wastewater was carried out. Sedimentation characteristics of the activated sludge were achieved after the second st...

The aim of this work was to evaluate the larvicidal activity of the chloroform extract of Senna crotalarioides against S. frugiperda. The chloroform extract of S. crotalarioides caused significant larval mortality, and reduced pupal weight and adult emergence. The analysis by gas chromatography coupled with mass spectrometry (GC-MS) revealed the pr...

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In Bacillus subtilis, the tRNACys lacks an encoded CCA 3′ end. To gain insight into the role of CCAase and RNases in tRNACys processing, several mutant strains were generated. Northern blot and RT-PCR results suggest that enzymes other than CCAase can participate in CCA addition at the 3′ end of the immature tRNACys.

American foulbrood (AFB) caused by Paenibacillus larvae is the most destructive honeybee bacterial disease and its dissemination via commercial bee pollen is an important mechanism for the spread of this bacterium. Because Mexico imports bee pollen from several countries, we developed a tRNACys-PCR strategy and complemented that strategy with MALDI...

En México, los cultivos de maíz y frijol se ven afectados por el ataque de insectos plaga como Spodoptera frugiperda, el uso de insecticidas químicos sintéticos para su control ha afectado el rendimiento de ambos cultivos. Una alternativa para reducir los daños causados por esta plaga en campo es el cultivo intercalado aunado con la agricultura org...

Bee pollen is a highly nutritive natural foodstuff. Because of its use as a comestible, the association of bacteria with bee pollen is commercially and biologically important. We report here the bacterial diversity of seven bee pollen samples (five from Europe, one from Chile, and one from Mexico) based on 16S rRNA gene amplicon metagenome sequenci...

Bacillus sp. strain MA04 a plant growth-promoting rhizobacteria (PGPR) showed hemolytic activity on blood agar plates, and the supernatant from liquid culture in nutrient broth at 24 h exhibited emulsification activity, suggesting the production of biosurfactants. In antagonist assays, the supernatant showed antifungal activity against phytopathoge...

Plant extracts can be used as an alternative to synthetic insecticides for the control of insect pests. Based on this knowledge, juvenomimetic and insecticidal activities of n-hexane extracts of the aerial parts of Senecio salignus DC. (Asteraceae) and Salvia microphylla Kunth (Lamiaceae) collected in Mexico were evaluated against 1st instar larvae...

The application of enzymatic extracts and conidia of Beauveria bassiana in Metama-sius spinolae and Cyclocephala lunulata was evaluated. The variables were mortality and time of death. In M. spinolae, mortality with extracts 29%, conidia 27% and the combination of both 31%, all had a time of death of four days. Although with different symptoms, use...

In order to evaluate the presence of heavy metals, samples of seawater were taken over one year, covering the four seasons, at three sites along a 100km section of the Mexican Pacific Ocean coastline, which extends for 7338km. The central point was Playa Blanca, Guerrero, while the two extremes of the sampling area are open sea regions (El Calvario...

In this work, mercury-resistant bacterial strains were isolated from the rhizosphere of an apple orchard, growing in a soil with high levels of mercury (Nuevo San Joaquin, Queretaro State, Mexico). Analysis of the soil in this region by the CVAAS method showed that it contained 637±51 mg mercury per kg. Mercury accumulation by fresh apples from thi...

The effect of four nitrogen levels (KNO3: 5, 10, 15 and 20 meq L-1) on the production of ricinine was studied using a semi-hydroponic system. The insecticidal activity of methanolic extracts of Ricinus communis L. leaves against Spodoptera frugiperda Smith larvae was also tested. A dosage _ response relationship showed strong positive correlation (...

The effect of four nitrogen levels (KNO3: 5, 10, 15 and 20 meq L-1) on the production of ricinine was studied using a semi-hydroponic system. The insecticidal activity of methanolic extracts of Ricinus communis L. leaves against Spodoptera frugiperda Smith larvae was also tested. A dosage -response relationship showed strong positive correlation (R...

RESUMEN. El maíz es el principal cultivo y fuente de alimentación en México, sin embargo presenta grandes daños y pérdidas económicas debido a diversas plagas. Una de ellas es el gusano cogollero del maíz Spodoptera frugiperda. Para su control se usan insecticidas químicos sintéticos de manera excesiva e indiscriminada, trayendo como consecuencia u...

RESUMEN. El gusano cogollero del maíz Spodoptera frugiperda (Lepidoptera-Noctuidae) es una plaga de cultivos del continente americano, en especial del maíz. Dicha problemática ocasiona que los productores recurran a insecticidas químico-sintéticos, acción que repercute en la salud pública y el medio ambiente, estos problemas han despertado el inter...

RESUMEN. El gusano cogollero del maíz, Spodoptera frugiperda (Lepidoptera: Noctuidae) es el insecto plaga con mayor importancia a tal cultivo, para su control se utilizan diferentes métodos, pero lo común es el uso de insecticidas químicos. Con el propósito de utilizar una alternativa natural para su manejo, como los productos vegetales, se evaluó...

RESUMEN. Los insecticidas botánicos mediante su correcta aplicación pueden resultar eficientes en el manejo de insectos plaga. Varios compuestos químicos que son sintetizados por las plantas se han aislado para su estudio ya sea como extractos o como sustancias puras ofreciendo a la vez un menor impacto ambiental que los plaguicidas sintéticos. Tal...

Resumen Las plantas producen una gran cantidad y diversidad de compuestos orgánicos que usan como mecanismos de defensa contra depredadores. El gusano cogollero (Spodoptera frugiperda) es el insecto plaga con mayor impacto en el cultivo de maíz; ya que puede llegar a ocasionar la pérdida total del cultivo, esta plaga se distribuye en las regiones t...

Essential oils can be used as an alternative to using synthetic insecticides for pest management. Therefore, the insectistatic and insecticidal activities of the essential oil of aerial parts of Salvia ballotiflora (Lamiaceae) were tested against the fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae). The results demonstrated insecticidal...

Resumen. La producción y calidad del nopal son seriamente afectadas por la incidencia Metamasius spinolae (Gyllenhal); algunas alternativas para el manejo de esta plaga son mediante la búsqueda de sus enemigos naturales. Se recolecta-ron un total de 130 puparios de M. spinolae en cultivo de nopal, en Zapotitlán, Tláhuac, Distrito Federal. Después d...

RESUMEN: Con el propósito de encontrar alternativas para el control de plagas, los productos naturales derivados de las plantas, pueden convertirse en una alternativa importante para el control de insectos nocivos en la agricultura. El extracto clorofórmico de la jarilla (Senecio salignus) tuvo efecto insecticida sobre larvas de Spodoptera frugiper...

RESUMEN: Con el propósito de encontrar alternativas para el control de plagas, los productos naturales derivados de las plantas, pueden convertirse en una alternativa importante para el control de insectos nocivos en la agricultura. El extracto clorofórmico de la jarilla (Senecio salignus) tuvo efecto insecticida sobre larvas de Spodoptera frugiper...

RESUMEN: Se evaluaron conidios y extractos enzimáticos de B. bassiana en larvas de Cyclocephala lunulata. Las larvas se colectaron en la UAM-X, se estableció un bioensayo con 6 tratamientos y 10 repeticiones. Hubo100% de mortalidad en los tratamientos B, C, D. Excepto para los F y G con un 60 y 90% respectivamente, existiendo diferencia significati...

The damage caused by the fall armyworm Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) to vegetative-stage maize cultivated with chemical nitrogen fertilizers, vermicompost, and Carica papaya L. (Caricaceae) seed extract was estimated. Each shoot was infested with one first instar larva of S. frugiperda. The variables measured included the per...

Introducción Los hongos entomopatógenos son importantes agentes en el control natural de insectos, que pueden ser utilizados con fun-gicidas en el manejo integrado de plagas (D'Ales sandro et al., 2011). Beau veria bassiana, B. brongniartii y Metarhizium anisopliae son los hongos ma-yormente estudiados y utiliza-dos ampliamente en el combate de ins...

There are more than 20 known species of entomopathogenic fungi capable of infecting the whitefly Bemisia tabaci. This paper shows the results of the assessment of microbiological potential of I. fumosorosea on the control of B. tabaci. Five application of the fungus were made at seven day intervals, at a rate of 5×109 conidia/g. The susceptibilitie...

The entomopathogenic fungi are subject to regulation system known as signal transduction that regulates the expression of the different genes required for each life stage, first comprising cuticle adhesion and degradation, later the survival in the hemolymph, and finally the fungal growth in soils. There are two signal-transduction mechanisms ident...

Chloroform extracts from the aerial parts of four species of medicinal plants of the genus Salvia (Lamiaceae) were tested for insectistatic and insecticidal activities against the life cycle of the fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae). All extracts showed both activities. Extracts from Salvia microphylla Kunth...

Beauveria bassiana, Metarhizium anisopliae and Isaria fumosorosea are entomopathogenic fungi used as biological control agents for whitefly. They have been individually evaluated under laboratory, greenhouse and field conditions; however, there is little information on their comparative efficacy under greenhouse conditions particularly with respect...

The discovery and development of insecticides isolated from natural products is a growing area of agricultural research. Here we show that kramecyne, is cyclic peroxide obtained from Krameria cytisoides, has insecticidal activity against Spodoptera frugiperda. The insecticidal and insectistatic activities of kramecyne against S. frugiperda were tes...