Konstantin V Popov

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I.Kulakov · Laboratory for personalized medicines

The toxic effects of four cationic porphyrins on various human cells were studied in vitro. It was found that, under dark conditions, porphyrins are almost nontoxic, while, under the action of light, the toxic effect was observed starting from nanomolar concentrations. At a concentration of 100 nM, porphyrins caused inhibition of metabolism in the...

Hydrophobic molecules may be toxic when present in excess. When dissolved in membranes, hydrophobic molecules disrupt membrane function. Studies on the effects of free fatty acids (FFA) on cultured cells contradict each other. Here we describe the effects of FFA on various human cells in culture. The addition of long-chain FFA (oleic, palmitic, lin...

In this study, the peculiarities of the meiotic cell cycle, chromosomal distribution of C-heterochromatin and also transcriptionally active nucleolus organizer regions (NORs) were investigated in the plants of Pisum sativum L. and Triticum compactum Host. derived from the seeds which were previously grown in the space greenhouse “Lada” on board the...

The paper contains a brief overview of full-text clustering methods for current research directions detection. A novel full-text clustering method is proposed. A dataset is created and experimental results are verified by problem domain “Regenerative medicine” experts with PhD degrees. The proposed method is well applicable for research directions...

This paper provides an overview of the methods and systems that are applied for scientometric analysis of scientific publications. Methods to identify promising research directions are described. The results of an experimental study aimed at determining the directions of scientific research within the subject area of “regenerative medicine” by usin...

The paper provides an overview of systems for scientometric analysis and considers methods for identifying current research directions. The new method for research directions detection based on full-text clustering is proposed. The results of experiments on the detection of the directions of the field "regenerative medicine" are presented. The pros...

Preparation stimulating hair growth (PSHG) was studied on mice of various strains (Balb/c, CBA, C57BI/6, and outbred). It was shown that a long-term (44 months) application of PSHG does not reliably affect the appearance of young healthy mice but does induce increase in the hair follicle size. No adverse consequences of the PSHG application were ob...

To estimate patients risks and make clinical decisions, evidence based medicine (EBM) relies upon the results of reproducible trials and experiments supported by accurate mathematical methods. Experimental and clinical evidence is crucial, but laboratory testing and especially clinical trials are expensive and time-consuming. On the other hand, a n...

We studied the properties of human skin fibroblast in filamentous polyglycolic microtransplant. Fibroblast adhesion to the microtransplant filaments is followed by the formation of a network cross-linked with fibroblasts. The cells rapidly proliferate during the first few days; after transfer of the microtransplant to the standard culture flask, th...

The comparison of chromosomes stained by the modified C- and OR-banding techniques in karyotypes of three varieties of Matricaria chamomilla L. was carried out by image analysis. Nine pairs of the C- and OR-banded chromosomes of camomile were classified according to their banding patterns, sizes and centromeric indexes in karyotypes of varieties Az...

Human cell aging is irregular. Senescent cells often impair the function of their neighbour nonsenescent cells. Stimulation of tissue regeneration is of potential applied significance for medicine, in particular, for geriatrics. One of the possible ways to induce regeneration consists of a selective removal of senescent and damaged cells from the t...

We studied the influence of three derivatives of pyrido[1,2-a]benzimidazoles (PBIs), which have DNA-intercalating properties, on plant mitotic chromosome condensation, in order to increase the resolution of chromosome analysis. The efficiency of the influence of these agents was assessed using the median chromosome length on chromosome slides, as w...

A comparative cytogenetic study of two introduced forms of Makleaya cordata (Willd.) R. Br. = syn. Bocconia cordata Willd. grown in different ecological and geographical regions (Moscow and Donetsk areas) was carried out. In the study, a complex of methods utilizing various chromosomal markers, i.e., C- and DAPI-banding technique, fluorescence in s...

Using a set of approaches based on the use of molecular cytogenetic markers (DAPI/C-banding, estimation of the total area of DAPI-positive regions in prophase nuclei, FISH with 26S and 5S rDNA probes) and the microsatellite (SSR-PCR) assay, we studied genomic polymorphism in 15 flax (Linum usitatissimum L.) varieties from different geographic regio...

C banding, Ag-NOR staining, FISH with pTa71 (45S rDNA) and pTa794 (5S rDNA), and RAPD-PCR analysis were used to study the genome and chromosome polymorphism in four varieties (Frisson, Sparkle, Rondo, and Finale) and two genetic lines (Sprint-2 and SGE) of pea Pisum sativum L. A comparison of the C-banding patterns did not reveal any polymorphism w...

Ten new fluorescent dyes have been tested as possible tools for human and plant chromosome banding; eight of the tested dyes were dimer derivatives of bisbenzimidazole (Hoechst 33248). The dimer compounds differed from each other by the length of the linker connecting benzimidazole moieties and by the structure of the moieties themselves. Four comp...

Karyotypes of species sects. Linum and Adenolinum have been studied using C/DAPI-banding, Ag-NOR staining, FISH with 5S and 26S rDNA and RAPD analysis. C/DAPI-banding patterns enabled identification of all homologous chromosome pairs in the studied karyotypes. The revealed high similarity between species L. grandiflorum (2n = 16) and L. decumbens b...

Comparative statistical analysis of total C-heterochromatin content (total area of darkly stained C-bands) in prophase nuclei and metaphase chromosomes of eight flax (Linum usitatissimum L.) varieties, viz., three oil flax varieties (Shafir, Oliver, Linotte), three modern (Orshansky-2, Belinka, Baltuchai) and two ancient fiber flax varieties (Zaret...

A comparative statistical analysis of heterochromatin content (total area of darkly stained C-bands) in prophase nuclei and metaphase chromosomes of eight flax varieties (Linum usitatissimum L.) has been carried out. The karyotypes of the flax varieties selected for different agronomical traits were found to differ in heterochromatin content. The t...

We synthesized dimeric Hoechst dye molecules composed of two moieties of Hoechst 33258 fluorescent dye with the phenolic hydroxy groups tethered via pentamethylene, heptamethylene, or triethylene oxide linkers. A characteristic pattern of differential staining of chromosome preparations from human HL60 premonocytic leukemia cells was observed for a...

We synthesized dimeric Hoechst dye molecules composed of two moieties of the Hoechst 33258 fluorescent dye phenolic hydroxy groups of which were tethered via pentamethylene, heptamethylene, or triethylene oxide linkers. A characteristic pattern of differential staining of chromosome preparations from human premonocytic leukemia HL60 cells was obser...

The plant sequences homologous to telomere repeat were localized on Linum usitatissimum L. and L. hirsutitm L. subsp. hirsutitm chromosomes using FISH method. It was found that under the stringent hybridization conditions only one clear interstitial signal in one chromosome pair of L. usitatissimum uas observed. After softening of hybridization con...

5'-Hydrogenphosphonate of 3'-azido-2',3'-dideoxythymidine (HpAZT), a novel anti-HIV drug approved for the treatment of HIV-infected patients in Russia, displays some clinical advantages over azidothymidine (AZT). Metabolism in the HL-60 cell culture and pharmacokinetics in mice of [6-3H]-HpAZT (in comparison with [6-3H-AZT) were studied to elucidat...

Fluorescence in situ hybridization (FISH) was for the first time used to study the chromosomal location of the 45S (18-2.5S-26S) and 5S ribosomal genes in the genomes of five flax species of the section Linum (syn. Protolinum and Adenolinum). In L. usitatissimum L. (2n = 30), L. angustifolium Huds. (2n = 30), and L. bienne Mill. (2n = 30), a major...

Chromosome C-banding patterns were analyzed in three closely related flax species (Linum usitatissimum L., 2n = 30; L. angustifolium Huds., 2n = 30; and L. bienne Mill., 2n = 30) and their hybrids. In each case, the karyotype included metacentrics, submetacentrics, and one or two satellite chromosomes. Chromosomes of the three flax species were sim...

Chromosome structure and chromatin organisation of a two-chromosome model cereal Zingeria biebersteiniana (Claus) P. Smirnov were studied: nuclear DNA content was determined by microdensitometric analysis after Feulgen staining; Feulgen absorption at different thresholds of absorbance in interphase nuclei also provided evidence on the organisation...

Chromosome C-banding patterns were analyzed in three closely related flax species (Linum usitatissimumL., 2n = 30; L. angustifolium Huds., 2n = 30; and L. bienneMill., 2n = 30) and their hybrids. In each case, the karyotype included metacentrics, submetacentrics, and one or two satellite chromosomes. Chromosomes of the three flax species were simil...

Successful automated chromosome analysis requires the development of new techniques to increase and standardize chromosome length and improve banding patterns. Human and plant cells were pretreated with the DNA intercalator 9-aminoacridine (9-AMA), and chromosomes were stained with GTG and aceto-orcein banding techniques and investigated by an imag...

Macrophage-like development of myeloid leukemia cells which can be induced by agents such as phorbol esters (TPA) is accompanied by integrin expression and cell adhesion. Thus, in differentiating myeloid leukemia cells CD11b is predominantly expressed which can associate with CD18 to form the functional heterodimeric integrin Mac-1. To elucidate th...

To estimate the possibility of plant genome mapping using human genome probes, the probes fluorescent in situ hybridization (FISH) of human 18S-28S rDNA (clon 22F9 from the LA-13NCO1 library) was carried out on chromosomes of the spring barley Hordeum vulgare L. As a control, wheat rDNA probe (clon pTa71) was taken. Hybridization of the wheat DNA p...

Visual analysis of small chromosomes of plants is difficult in most cases, because the chromosomes are similar in morphology and banding patterns. The computer software applied to chromosome analysis extended the capabilities of karyotypic analysis of small-chromosome species, because precise mathematical parameters of chromosomes and chromosome ba...

The comparison of chromosomes stained by the modified C- and OR-banding techniques in karyotypes of three varieties of Matricaria chamomilla L. was carried out by image analysis. Nine pairs of the C- and OR-banded chromosomes of camomile were classified according to their banding patterns, sizes and centromeric indexes in karyotypes of varieties Az...

C-banding patterns of the karyotypes of two closely related wild flax species, Linum austriacum L. (2n = 18) and Linum grandiflorum Desf. (2n = 16), were studied. The karyotypes of both species were similar in the chromosome morphology and size. In each species, metacentric and acrocentric chromosomes (1.7-4.3 microns) and one satellite chromosome...

C-banding patterns of the karyotypes of two closely related wild flax species, Linum austriacumL. (2n= 18) and Linum grandiflorumDesf. (2n= 16), were studied. The karyotypes of both species were similar in the chromosome morphology and size. In each species, metacentric and acrocentric chromosomes (1.7–4.3 m) and one satellite chromosome were obser...

The C-banding technique was used to study flax chromosomes (Linum usitatissimum L., 2n = 30). Heterochromatin was located mainly in pericentromeric regions of chromosomes. In spite of small size (1.5-3.5 microm), all 15 pairs of homologous chromosomes were identified on the basis of the C-banding pattern and morphology. An idiogram of C-banded chro...

To estimate the possibility of plant genome mapping using human genome probes, the probes fluorescent in situ hybridization (FISH) of human 18S–28S rDNA (clon 22F9 from the LA-13NCO1 library) was carried out on chromosomes of the spring barleyHordeum vulgareL. As a control, wheat rDNA probe (clon pTa71) was taken. Hybridization of the wheat DNA pro...

We showed that the telomerase activity (TAC) of human promyelocytic leukemic cells U-937 and HL-60 sharply decreased after induction of macrophagal differentiation. Dedifferentiation which occurred several days after removing the inductor was accompanied by the resumption of proliferation and increase of TAC. Telomerase activity significantly decre...