Frank Kleinjung

Bayer HealthCare · Bayer HealthCare Pharmaceuticals

Background Heart failure (HF) is a major cause of cardiovascular morbidity and mortality. Despite recent advances in diagnosis and management of HF, the prognosis remains poor. HF and chronic kidney disease (CKD) are interlinked chronic health conditions. The availability of large volume of patient data and modern analytic techniques opens new oppo...

Background Heart failure (HF) is a major cause of cardiovascular morbidity and mortality. Despite recent advances in diagnosis and management of HF, the prognosis remains poor. HF and chronic kidney disease (CKD) are interlinked chronic health conditions. The availability of large volume of patient data and modern analytic techniques opens new oppo...

Background: Pulmonary arterial hypertension restricts the ability of patients to perform routine physical activities. As part of pulmonary arterial hypertension treatment, inhaled iloprost can be administered via a nebulizer that tracks inhalation behavior. Pulmonary arterial hypertension treatment is guided by intermittent clinical measurements,...

Purpose The REPLACE study showed that switching to riociguat (rio) can benefit pulmonary arterial hypertension (PAH) patients treated with phosphodiesterase type 5 inhibitors (PDE5i) but still at intermediate risk. Improvements to lower risk status with rio versus PDE5i were seen with all three risk assessment tools analyzed, although the differenc...

Background Riociguat and phosphodiesterase-5 inhibitors (PDE5i), approved for the treatment of pulmonary arterial hypertension (PAH), act on the same pathway via different mechanisms. Riociguat might be an alternative option for patients with PAH who do not respond sufficiently to treatment with PDE5i, but comparisons of the potential benefits of r...

Evaluación del resultado compuesto del estudio REPLACE en el estudio PATENT

Assessment of the REPLACE study composite endpoint in the PATENT

BACKGROUND Pulmonary arterial hypertension restricts the ability of patients to perform routine physical activities. As part of pulmonary arterial hypertension treatment, inhaled iloprost can be administered via a nebulizer that tracks inhalation behavior. Pulmonary arterial hypertension treatment is guided by intermittent clinical measurements, su...

The goal of treatment in patients with pulmonary arterial hypertension is to achieve a low risk status, indicating a favorable long-term outcome. The REPLACE study is investigating the efficacy of switching to riociguat in patients with pulmonary arterial hypertension and an insufficient response to phosphodiesterase-5 inhibitors. In this post hoc...

The study aims to develop an approach to predict the estimated glomerular filtration rate (eGFR) level based on longitudinal patient data in the absence of lab measures and identify predictive items (demographic characteristics, diagnoses and procedure codes, therapies) using data-driven analytic techniques.

Inhaled iloprost is an effective therapy for patients with pulmonary arterial hypertension (PAH); however, some patients experience extended inhalation times when using the V10 formulation (10.0 µg/mL) to deliver a 5 -µg dose (at mouthpiece) and are at risk of incomplete inhalations and reduced inhalation frequency. VENTASWITCH was an observational...

The purpose of this study was to analyze potential influences of magnetic resonance (MR) on the course of automatically device based assessed lead parameters remotely transmitted in patients who were implanted with MR conditional permanent pacemakers and who had non-diagnostic brain and lumbar spine MR (1.5T) within the ProMRI single center pilot s...

A Tool for Quantitative Assessment of Dioxin Contamination

Recent development of novel technologies paved the way for quantitative proteomics. One of the most important among them is iTRAQ, employing isobaric tags for relative or absolute quantitation. Despite large progress in technology development, still many challenges remain for derivation and interpretation of quantitative results. One of these chall...

Examples for Potential Isoforms. Additional zip archive containing pdf images for 6 further examples with potential protein isoforms.

Diabetes like many diseases and biological processes is not mono-causal. On the one hand multi-factorial studies with complex experimental design are required for its comprehensive analysis. On the other hand, the data from these studies often include a substantial amount of redundancy such as proteins that are typically represented by a multitude...

Peak alignment. Visualization of the results of the peak alignment method. The heuristic algorithm used for peak alignment is presented in pseudo-code.

Results for Genotype. Scatter plot of peak intensity values for peaks 3388 and 5029 and peak intensities profile for peak 3388. The peaks are in the list of the most significant results for the experimental factor genotype.

Crossbreeding studies in rodents have identified numerous quantitative trait loci (QTL) that are linked to diabetes-related component traits. To identify genetic consensus regions implicated in insulin action and glucose homeostasis, we have performed a meta-analysis of genomewide linkage scans for diabetes-related traits. From a total of 43 publis...

Functional proteomics aims to describe cellular protein networks in depth based on the quantification of molecular interactions. In order to study the interaction of adenosine-3',5'-cyclic monophosphate (cAMP), a general second messenger involved in several intracellular signalling networks, with one of its respective target proteins, the regulator...

A variety of different in vivo and in vitro technologies provide comprehensive insights in protein-protein interaction networks. Here we demonstrate a novel approach to analyze, verify and quantify putative interactions between two members of the S100 protein family and 80 recombinant proteins derived from a proteome-wide protein expression library...

The borderline between molecular recognition element (MRE) tools that are biological in nature and synthetic (organic) receptor molecules is no longer well definable; these two classes of recognition elements are merging. This is especially true for enzyme models, polymers imprinted by biomolecules, ionophores (which mimic the function of channels)...

The neuropeptide nociceptin/orphanin FQ (N/OFQ), the endogenous ligand of the opioid receptor-like 1 (ORL1) receptor, has been shown to play a prominent role in the regulation of several biological functions such as pain and stress. Here we describe the isolation and characterization of N/OFQ binding biostable RNA aptamers (Spiegelmers) using a mir...

Synthesis of 2'-fluoro-nucleosides from L-arabinose in order to perform the synthesis of 2'-fluoro-Spiegelmers binding to a neuropeptide.

Bacterial staphylococcal enterotoxin B is involved in several severe disease patterns and it was therefore used as a target for the generation of biologically stable mirror‐image oligonucleotide ligands, so called Spiegelmers. The toxin is a 28 kDa protein consisting of 239 amino acids. Since the full‐length protein is not accessible to chemical pe...

Spiegelmers are high-affinity l-enantiomeric oligonucleotide ligands that display high resistance to enzymatic degradation compared with d-oligonucleotides. The target binding properties of Spiegelmers can be designed by an in vitro-selection process starting from a random pool of oligonucleotides. Applying this method, a Spiegelmer with high affin...

Binding and catalytic activity of the type II restriction endonuclease EcoRI on immobilized DNA has been observed in real time using three different evanescent wave biosensors and two different immobilization techniques. The method gives direct access to the turnover number (k cat ) without the necessity for the determination of any concentration o...

The appeal of microarray technology is the possibility of large-scale parallel determination of a variety of variables simultaneously. Hence, microarray technologies attract the interest of both the scientific and business worlds alike. High-throughput screening has been the major focus of the utilization of microarray technologies in recent years,...

Small deviations in gene sequences are coming in the focus of interest since the influence of SNPs (single nucleotide polymorphisms) on medical therapy is better understood. We now present a detection method for SNPs, which is robust in respect to noisy samples, i.e. variation of DNA concentration or interfering DNA. The method is based on the dete...

A method is presented to modify surfaces for biotechnological applications. Oligonucleotides have been coupled covalently to a pre-activated surface. Complementary oligonucleotides hybridize to the surface, which are conjugated with functionalities. The oligonucleotides serve as "Oligo-Tags" for these functionalities that now are linked specificall...

Neue Wechselwirkungspartner einer PDZ-Proteindomäne konnten anhand einer Bibliothek identifiziert werden, die aus allen bekannten C-terminalen Peptiden („P” in der schematischen Darstellung) humaner Proteine besteht. Die Bibliothek bestand aus ortsgerichtet an eine Cellulosemembran gebundenen, invertierten Peptiden.

New interaction partners of a PDZ protein domain were identified through use of a library made up of all known human protein C-terminal peptides (“P” in the schematic representation). The library was displayed on a cellulose membrane by positional resolution of inverted peptides.

A biosensor for l-adenosine employing high-affinity RNA as binder is reported. Real-time measurement is obtained using total internal reflection fluorescence in a fiber-optic format. High-affinity RNA was attached to the core of a multimode fiber via an avidin−biotin bridge. The sensor measures binding of FITC-labeled l-adenosine. The interaction w...

The binding of DNA oligonucleotides to immobilized DNA-targets using a fibre optic fluorescence sensor is demonstrated. 13mer oligonucleotides were attached to the core of a multimode fibre. The complementary sequence was detected by use of a fluorescent double strand specific DNA ligand (YOYO and PicoGreen). The evanescent field was employed to di...

Nucleic acids are used as receptors in biosensing for the determination of complementary nucleic-acid strands. This may be useful in clinical diagnostics, e.g., searching for DNA from viruses, or in other fields of hygiene or environmental monitoring. In this study we demonstrate the reversible binding of DNA oligonucleotides to immobilized DNA tar...