Carnesecchi JulieFrench National Centre for Scientific Research | CNRS · The Institute of Molecular Genetics of Montpellier (IGMM)
Carnesecchi Julie
PhD
Understanding the molecular mechanisms of cell fate decision
About
31
Publications
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Introduction
Integrative mRNA processing and transcription regulatory networks
Additional affiliations
December 2021 - August 2024
July 2021 - November 2021
May 2015 - June 2021
Publications
Publications (31)
During development cells become gradually restricted in their differentiation potential by the repression of alternative cell fates. While we know that the Polycomb complex plays a crucial role in this process, it still remains unclear how alternative fate genes are specifically targeted for silencing in different cell lineages. We address this que...
Hox transcription factors (TFs) function as key determinants in the specification of cell fates during development. They do so by triggering entire morphogenetic cascades through the activation of specific target genes. In contrast to their fundamental role in development, the molecular mechanisms employed by Hox TFs are still poorly understood. In...
Transcription factors (TFs) control cell fates by precisely orchestrating gene expression. However, how individual TFs promote transcriptional diversity remains unclear. Here, we use the Hox TF Ultrabithorax (Ubx) as a model to explore how a single TF specifies multiple cell types. Using proximity-dependent Biotin IDentification in Drosophila, we i...
Transcription factors (TFs) play a pivotal role in cell fate decision by coordinating gene expression programs. Although most TFs act at the DNA layer, few TFs bind RNA and modulate splicing. Yet, the mechanistic cues underlying TFs activity in splicing remain elusive. Focusing on the Drosophila Hox TF Ultrabithorax (Ubx), our work shed light on a...
Transcription factors (TFs) are present in all life forms and conserved across great evolutionary distances in eukaryotes. From yeast to complex multicellular organisms, they are pivotal players of cell fate decision by orchestrating gene expression at diverse molecular layers. Notably, TFs fine‐tune gene expression by coordinating RNA fate at both...
Hox genes encode for evolutionary conserved transcription factors that have long fascinated biologists since the observation of the first homeotic transformations in flies. Hox genes are developmental architects that instruct the formation of various and precise morphologies along the body axes in cnidarian and bilaterian species. In contrast to th...
Hox proteins have similar binding specificities in vitro, yet they control different morphologies in vivo. This paradox has been partially solved with the identification of Hox low-affinity binding sites. However, anterior Hox proteins are more promiscuous than posterior Hox proteins, raising the question how anterior Hox proteins achieve specifici...
Transcription Factors (TFs) play a pivotal role in cell fate decision by coordinating distinct gene expression programs. Although most TFs act at the DNA regulatory layer, few TFs can bind RNA and modulate mRNA splicing. Yet, the mechanistic cues underlying TFs function in splicing remain elusive. Focusing on the Drosophila Hox TF Ultrabithorax (Ub...
Hox proteins are major regulators of embryonic development, acting in the nucleus to regulate the expression of their numerous downstream target genes. Analyzing deleted forms of the Drosophila Hox protein Ultrabithorax (Ubx) identified the presence of an unconventional Nuclear Export Signal (NES) that overlaps with a highly conserved motif origina...
Studying the spatiotemporal control of gene regulatory networks at the single-cell level is still a challenge, yet it is key to understanding the mechanisms driving cellular identity. In their recent study, Aerts and colleagues (González-Blas et al, 2020) develop a new strategy to spatially map and integrate single-cell transcriptome and epigenome...
Hox proteins are major regulators of embryonic development, acting in the nucleus to regulate the expression of their numerous downstream target genes. By analyzing deleted forms of the Drosophila Hox protein Ultrabithorax (Ubx), we revealed the presence of an unconventional Nuclear Export Signal (NES) that overlaps with the highly conserved hexape...
During development cells become restricted in their differentiation potential by repressing alternative cell fates, and the Polycomb complex plays a crucial role in this process. However, how alternative fate genes are lineage-specifically silenced is unclear. We studied Ultrabithorax, a multi-lineage transcription factor of the Hox class, in two t...
Primers used for qPCR experiments to test total RNA.
Primers used for qPCR to test genomic loci identified by ChIP experiments.
Collection of GO terms grouped into different categories for multiple GO term testing.
Table of known genes expressed in the mesodermal or neuronal lineages.
Lysine-specific demethylase 1 (LSD1) exerts dual effects on histone H3, promoting transcriptional repression via Lys4 (H3K4) demethylation or transcriptional activation through Lys9 (H3K9) demethylation. These activities are often exerted at transcriptional start sites (TSSs) and depend on the type of enhancer-bound transcription factor (TFs) with...
The LSD1 histone demethylase is highly expressed in breast tumors where it constitutes a factor of poor prognosis and promotes traits of cancer aggressiveness such as cell invasiveness. Recent work has shown that the Estrogen-Related Receptor α (ERRα) induces LSD1 to demethylate the Lys 9 of histone H3. This results in the transcriptional activatio...
Specificity of ERRα protection by LSD1.
a. Detection of the indicated proteins in MDA-MB231 cells after treatment with siRNAs. Quantifications of AR and ERRα levels (relative to actin) are displayed. b. Co-immunoprecipitation of endogenous proteins with anti-AR or anti-ERRα antibody (or rabbit IgG as control) from MDA-MB231 cells. Note that the 100...
ERRα protein is stabilized by LSD1 in a proteasome dependent manner.
a. Analysis of ERRα and LSD1 protein levels in HeLa cells at the indicated time after siLSD1 transfection. b. Analysis of ERRα and LSD1 protein levels in HeLa cells after the indicated siRNA transfection and treatment with MG132 or vehicle. c. Expression of the indicated genes ana...
Significance
Dynamic demethylation of histone residues plays a crucial role in the regulation of gene expression. Lysine Specific Demethylase 1 (LSD1) can remove both transcriptionally permissive and repressive histone marks. How these activities are controlled is not clearly understood. Here, we show that the estrogen-related receptor α (ERRα) ind...
The post-menopausal decrease in estrogen circulating levels results in rapid skin deterioration pointing out to a protective effect exerted by these hormones. The identity of the skin cell type responding to estrogens is unclear as are the cellular and molecular processes they elicit. Here, we reported that lack of estrogens induces rapid re-organi...
Significance
Several developmental and physiological processes require that cells display a controlled ability to migrate in an orientated manner. This capacity is also reacquired by certain cancer cells during their progression toward aggressiveness that allows them to establish distant metastases. The Rho GTPases are instrumental in the control o...
The estrogen-related receptors (ERRα, β, and γ) are orphan members of the nuclear receptor superfamily. ERRα and γ are highly expressed in tissues displaying elevated energy demands and are involved in several aspects of energetic metabolism, which they regulate mostly in association with members of the PGC-1 coactivator family. These activities ha...
ERRα is an orphan member of the nuclear receptor family, the complete inactivation of which confers resistance to bone loss induced by ageing and estrogen withdrawal to female mice in correlation with increased bone formation in vivo. Furthermore ERRα negatively regulates the commitment of mesenchymal cells to the osteoblast lineage ex vivo as well...
3D reconstitution of trabecular bone originating from control sham female mice.
(AVI)
3D reconstitution of trabecular bone using control ovx mice.
(AVI)
3D reconstitution of trabecular bone using cKO sham mice.
(AVI)
Questions
Questions (2)
I want to construct a plasmid (for Drosophila cell system) containing a intron in order to study splicing process. In detail, I would like to insert wether a weak or a strong splice donor site (followed by small intron+ splice acceptor) in the firefly luciferase to be able to easy monitor the splicing activity by standard luciferase assay. However, if I can find easily the consensus site for 5'SS and 3'SS, I am struggling to find the appropriate full DNA sequences that I would like to practically insert in my plasmid. Is there someone that can help me finding this sequence? (addgene reference? detailled publication with the DNAsequence fully available ?)
Thanks in advance for your help,
J
