Alain Rouleau

Institut FEMTO-ST | FEMTO ST · Department of Micro Nano Sciences and Systems (MN2S)

Despite numerous innovations, measuring bacteria concentrations on a routine basis is still time consuming and ensuring accurate measurements requires careful handling. Furthermore, it often requires sampling small volumes of bacteria suspensions which might be poorly representative of the real bacteria concentration. In this paper, we propose a sp...

Advanced Therapy Medicinal Products are promising drugs for patients in therapeutic impasses. Their complex fabrication process implies regular quality controls to monitor cell concentration. Among the different methods available, optical techniques offer several advantages. Our study aims to measure cell concentration in real time in a potential c...

Background Caveolae are invaginated plasma membrane domains of 50–100 nm in diameter involved in many important physiological functions in eukaryotic cells. They are composed of different proteins, including the membrane-embedded caveolins and the peripheric cavins. Caveolin-1 has already been expressed in various expression systems ( E. coli, inse...

Background: Caveolae are invaginated plasma membrane domains of 50-100 nm in diameter involved in many important physiological functions in cells. They are composed of different proteins, including the membrane-embedded caveolins and the peripheric cavins. Caveolin-1 has already been expressed in various expression systems (E. coli, insect cells, T...

Primary haemostasis is a complex dynamic process, which involves in-flow interactions between platelets and sub-endothelial matrix at the area of the damaged vessel wall. It results in a first haemostatic plug, which stops bleeding, before coagulation ensues and consolidates it. The diagnosis of primary haemostasis defect would benefit from evaluat...

LBS03.02- introduction: Custom-made Platelet Pellet Lysate (PPL) and Heat-treated PPL (HPPL) exert strong neuroprotective effects of neurotoxin-exposed dopaminergic LUHMES neuronal cell culture. This effect is significantly enhanced using HPPL, which was also highly protective of TH-expressing neurons in mice Parkinson’s disease (PD) model. The rol...

A general method to develop diazonium-based biochip for SPRi analysis is presented. The electrografting of carboxybenzene diazonium salt is optimized in order to fit with the requirement of the SPR technology. The influence of the surface preparation on plasmon quality is discussed and the performance of this original modified biochip is investigat...

The contribution focuses on the development of microresonant sensor solution integrated in microfluidic platform for the haemostasis assessment at realistic rheological flow conditions similar to the one in blood vessels. A multi-parameter sensor performs real time analysis of interactions between immobilized collagen and platelets. The detection a...

ABO mismatch between donor and patient’s blood is still the cause of accidents which are sometimes lethal. The main causes of mis-assignment are human errors and wrong identification of patients or blood product. Only a final compatibility test at the patient’s bedside can avoid these errors. In some countries, this test is performed using manual p...

In the field of blood transfusion, there is a need to improve the bedside pre-transfusion ABO compatibility test. In France, this test is mandatory for each red cell concentrates transfusion. It is performed manually and serious transfusion accidents still occur, principally due to human errors. Therefore, an automated ABO compatibility test is req...

Immuno-SPR-MS is the combination of immuno-sensors in biochip format with mass spectrometry. This association of instrumentation allows the detection and the quantification of proteins of interest by SPR and their molecular characterization by additional MS analysis. However, two major bottlenecks must be overcome for a wide diffusion of the SPR-MS...

Before each transfusion of red blood cell concentrate, a final ABO compatibility test is carried out at the patient's bedside on a piece of card and interpreted visually. Despite this ultimate test, transfusion accidents still occur due to group incompatibility, which can be lethal. In order to improve this test, we have developed a specific device...

Neuropilins, initially characterized as neuronal receptors, act as co-receptors for cancer related growth factors and were recently involved in several signaling pathways leading to cytoskeletal organization, angiogenesis and cancer progression. Then, we sought to investigate the ability of neuropilin-2 to orchestrate epithelial-mesenchymal transit...

ince rubella vaccine was established, cytomegalovirus infection has become the most frequent cause of congenital infections. The French Health Authority is urging a diagnosis at birth for newborns. Since no screening device is commercially available, a consortium has been established to set-up an original device. The project has been funded by the...

Peroxisome proliferators have been extensively studied in rodents and are known to induce liver tumors, whereas the effects of these compounds are not very clearly identified in humans when they are widely exposed to herbicides, plasticizers, solvents or drugs such as the lipid-lowering fibrate bezafibrate (BEZA). We assessed the effect of BEZA on...

Recently, cytomegalovirus (CMV) infection has become the most frequent cause of congenital infections. The French Health Authority (HAS) is urging a diagnosis at birth for newborns. Since no screening device is commercially available, a consortium has been established to set-up an original device. It consists of a disposable cartridge containing th...

Since rubella vaccine was established, cytomegalovirus (CMV) infection has become the most frequent cause of congenital infections. Before deciding of the benefit of screening in this populations, the French Health Authority (HAS) is urging a diagnosis at birth for newborns. Since no screening device is commercially available, a consortium has been...

A major drawback of protein microarrays is the lack of control of ligand immobilization at the surface of the chip which limits their performances and thus their impacts in in vitro diagnosis. To improve antibody (Ab) grafting during the spotting process on commercialized gold SPRi chips, we propose to produce a chaotic flow in every spotted drople...

During µarray building, the spotting mode leads to low efficiency in the ligand grafting and heterogeneities which limit its performances. To improve the transfer rate, we induced, with a home-made acoustic transducer, a hydrodynamic flow in every spotted droplet in order to disrupt the steady state which occurs during antibodies (Ab) grafting. Our...

Myeloid-derived suppressor cells (MDSCs) have been identified in humans and mice as a population of immature myeloid cells with the ability to suppress T cell activation. They accumulate in tumor-bearing mice and humans and have been shown to contribute to cancer development. Here, we have isolated tumor-derived exosomes (TDEs) from mouse cell line...

Orientation and three-dimensional structure of immobilized proteins on bio-devices are very important to assure their high performance. Time-of-flight secondary ion mass spectrometry (TOF-SIMS) is able to analyze upper surface of one layer of molecules. Orientation of immobilized proteins can be evaluated based on determination of a partial structu...

A great challenge in biosensors and diagnosis devices relies on the way to reconstitute relevant biological mechanisms on surface of the biochips and which analytical tools are convenient to provide accurate and rapid information on the structures and function of molecules attached to this surface. A better control in the realization of biochips ca...

Bezafibrate (BezaF), a lipid-lowering fibrate, belongs to a group of structurally very diverse compounds designated as peroxisome proliferators, which elicit pathological changes including proliferation of peroxisomes, cell proliferation, hepatomegaly and the development of carcinomas in rodents. In contrast, human hepatocytes do not exhibit these...

A simple method to prepare flat gold terraces on mica for atomic force microscopy biomolecular characterisation is described. The procedure includes preheating of the substrate, metal deposition and an annealing step. All of these steps are at elevated temperatures (300-420degC). This approach allows one to prepare large flat gold terraces (200-500...

We present the results of a study in which biomolecular interaction analysis (BIA, Biacoretrade mark 2000) was combined with mass spectrometry (MS) using entire "on-a-chip" procedure. Most BIA-MS studies included an elution step of the analyte prior MS analysis. Here, we report a low-cost approach combining Biacore analysis with homemade chips and...

We present herein an original approach, consisting to lead in parallel a global analysis (SPR) and nanoscale characterizations (AFM, mass spectrometry, TOF-SIMS) of bio-molecules on the biochip surface. The questions we want to answer concern especially the organization, orientation and identification of immobilized and captured biomolecules. In or...