How to prevent Silver Nitrate (AgNO3) precipitating in growth broth?

Hi All:

I have some published papers on record, however, my paper review history has been on the lower side, any suggestions on how to get more paper review invites, or directly contact the journal can also be a viable approach, if so, which journal is a good start? my interest areas include CNT synthesis/applications, carbon hybrid materials as well as their synthesis and applications, Thank you in advance.

Daniel

Hi, I'm currently doing research on the electrode materials of supercapacitors. I already plotted the XRD graph for FeCo₂O₄-cnt. It would help if I have a JCPDS card as a reference for my graph of FeCo₂O₄.

I have xrd data of my synthesized CuO and i want to compare it with standard peaks

My sample is a catalyst based on metal oxide(NiO, Ce2O3) I want to do TEM test, I was wondering how to prepare the sample for the TEM test?

Thank you.

Hi,

I have a problem regarding Bradford protein assay. When I dissolve Coomassie Brilliant blue G-250 (C.I. 42655) in 96% ethanol, the solution is blue. Upon adding phosphoric acid, it turns into red-brown. But, when I add water to make up to the final volume, the final solution is blue. Shouldn't that be a red-brown solution?

Many thanks for your comments.

Siavoush

Hello,

Greetings!

I am conducting a MDCK cell permeability assay.Below is the protocol I followed :

1. In the apical region, 100 ul cell suspension containing about 33,000 cells were seeded in each insert in 24 well transwell plate (6.5mm transwell insert diameter, 0.33 cm2 insert membrane growth area and 3um pore size).

2. 0.6 ml of media (EMEM with L-glutamine) was added in the basolateral region.

3. Media was replaced each day in both apical and basolateral region.

4. On 7th day, Lucifer dye assay was conducted. 3 wells were used for lucifer assay. Briefly, inserts (appeal region) and basolateral region were washed with prewarmed HBSS. 100ul of 100ug/ml of Lucifer dye solution in HBSS was added in the apical region. 600ul of HBSS was added in the basolateral region. Samples from basolateral region were collected after an hour and checked for the fluorescence.

Results showed no permeation of lucifer dye after an hour.

5. Now, for permeability assay, first, the inserts were washed with HBSS and then added 100 ul HBSS in apical and 600 ul in basolateral region and kept for 30 min in the incubator for equilibration.

6. 100 ul of nanoformulation and free drug control formulation (containing 100ug drug) was then added in each inserts and kept on the belly dancer (slow shaker to avoid the stagnant layer).

7. 200 ul Samples were then taken and replaced with fresh HBSS at 30 min, 1 hr, 2h, 3h, 4h and 24 hour.

8. All the samples were analyzed using LCMS.

9. Results showed no permeation at 30 min, 1 hr, 2h, 3h and 4h from both nano formulation and control formulation. However, it showed permeation at 24 hr with 2.23% from nano formulation and 1.22% from control.

10. Results also showed, about 3% drug was left in the apical region. Meaning, about 93% drug is stuck (or absorbed) in the cells.

Could anybody suggest if I am missing any step in the protocol?

Thank you so much.

I have synthesized ZnO and NiO nanoparticles with size around 40 and 35 nm respectively without any capping agent. Now they are in powder form. Please suggest me, how can I make stable dispersion of these NPs (individual)?

Please suggest from your experience.

Hi,

These measurements are new to me so apologies for the naive questions. How can I use profilometry or ellipsometry to measure the thickness of spin coated quantum dot films on CVD graphene (on a copper substrate)? Given the uniform coverage across the film, I do not have any obvious steps that would be well suited for profilometry. I saw a few papers use ellipsometry but I am not sure what would be the right fitting procedure to use to accurately measure the thickness. Any help in either of these directions would be very helpful!