University of East Anglia

Background One of the few studies to estimate infection risk with SARS-CoV-2 in the general population was the UK Office of National Statistics Infection Survey. This survey provided data that allowed us to describe and interpret apparent risk factors for testing positive for SARS-CoV-2 in a period when variants and COVID-19 controls experienced large changes. Method The ONS published estimates of likelihood of individuals testing positive in two week monitoring periods between 21st November 2021 and 7th May 2022, relating this positivity to social and behavioural factors. We applied meta-regression to these estimates of likelihood of testing positive to determine whether the monitored potential risk factors remained constant during the pandemic. Results Some risk factors had consistent relationship with risk of infection (always protective or always linked to higher risk, throughout monitoring period). Other risk factors had variable relationship with risk of infection, with changes seeming to especially correlate with the emergence of Omicron BA.2 dominance. These variable factors were mask-wearing habits, history of foreign travel, household size, working status (retired or not) and contact with children or persons age over 70. Conclusion Relevance of some risk factors to likelihood of testing positive for SARS-CoV-2 may relate to reinfection risk, variant infectiousness and status of social distancing regulations.

In this chapter, I will make a case that neuroscience can help with the understanding of any art, and that in the context of rock art, with its deep history, it offers particular advantages. Most importantly it can give us new access to the minds of its makers and users, something much needed in the absence of the verbal commentaries associated with most other categories of material. That access, I suggest, can be obtained by using the latest knowledge of the extent to which the formation of the individual brain is affected by the environment to which it is exposed. This knowledge can help not only to reconstruct salient aspects of the neural resources of any individual or group whose material and social environment is sufficiently familiar to us, but also to infer how those resources are likely to have influenced such art-related behaviours as their motor inclinations and visual preferences. When these insights are supported by an understanding of such other newly discovered properties of our brains as its neural plasticity and neural mirroring, we can build up a new understanding of the mental activities behind the similarities and the differences in the way people living at different places and times have marked rock walls. A neural approach also allows us to re-evaluate assumptions about the history of culture that have been taken for granted in the fields of archaeology, anthropology, and art history, such as the pre-eminence of the role of language in the formation of culture and the associated insistence that art is necessarily a symbolic activity. In this way neuroscience can add a new dimension to cultural history.

Recreational fishing is popular worldwide, but many low- and middle-income coun-tries (LMICs) are experiencing increased participation and reduced catches. Like other LMICs, the recreational fishery in Namibia is facing concerns regarding its sustain-ability. Empirical evidence suggested limited knowledge of the fish and fishery as one plausible cause. Herein, we used a horizon scan survey to identify critical questions by anglers and decision makers to facilitate sustainable and adaptive management strategies. Of 115 questions and concerns raised by 59 stakeholders, including fish-eries managers, anglers (specialists and non-specialists), and scientists, 58 of the top research questions were organised within 11 high- priority themes, including: govern-ance; human dimensions; regulatory actions; compliance; knowledge of fish popu-lations; resource monitoring and data acquisition; angler outreach, education, and engagement; competing sectors/groups; bioeconomics; catch-and- release practises and perceived threats. Questions raised by non-specialist anglers differed from those raised by specialist anglers and scientists, which highlighted the potential importance of integrating fisher ecological knowledge into fishery management. Although ques-tions were in the context of recreational fisheries, we recommend that some of the themes identified herein may be applicable to other LMICs and may also improve understanding of other fisheries, such as small-scale or commercial fisheries.

This research analyses managers’ perceptions of the multiple types of artificial intelligence (AI) required at each stage of the business‐to‐business (B2B) service recovery journey for successful human–AI collaboration in this context. Study 1 is an exploratory study that identifies managers’ perceptions of the main stages of a B2B service recovery journey based on human–AI collaboration and the corresponding roles of the human–AI collaboration at each stage. Study 2 provides an empirical examination of the proposed theoretical framework to identify the specific types of intelligence required by AI to enhance performance in each stage of B2B service recovery, based on managers’ perceptions. Our findings show that the prediction stage benefits from collaborations involving processing‐speed and visual‐spatial AI. The detection stage requires logic‐mathematical, social and processing‐speed AI. The recovery stage requires logic‐mathematical, social, verbal‐linguistic and processing‐speed AI. The post‐recovery stage calls for logic‐mathematical, social, verbal‐linguistic and processing‐speed AI.

Choice-based preference elicitation methods such as the discrete choice experiment (DCE) present hypothetical choices to respondents, with an expectation that these hypothetical choices accurately reflect a ‘real world’ health-related decision context and that consequently the choice data can be held to be a true representation of the respondent’s health or treatment preferences. For this to be the case, careful consideration needs to be given to the format of the choice task in a choice experiment. The overarching aim of this paper is to highlight important aspects to consider when designing and ‘setting up’ the choice tasks to be presented to respondents in a DCE. This includes the importance of considering the potential impact of format (e.g. choice context, choice set presentation and size) as well as choice set content (e.g. labelled and unlabelled choice sets and inclusion of reference alternatives) and choice questions (stated choice versus additional questions designed to explore complete preference orders) on the preference estimates that are elicited from studies. We endeavoure to instil a holistic approach to choice task design that considers format alongside content, experimental design and analysis.

Urbanization modifies ecosystem conditions and evolutionary processes. This includes air pollution, mostly as tropospheric ozone (O3), which contributes to the decline of urban and peri‐urban forests. A notable case are fir (Abies religiosa) forests in the peripheral mountains southwest of Mexico City, which have been severely affected by O3 pollution since the 1970s. Interestingly, some young individuals exhibiting minimal O3—related damage have been observed within a zone of significant O3 exposure. Using this setting as a natural experiment, we compared asymptomatic and symptomatic individuals of similar age (≤15 years old; n = 10) using histologic, metabolomic, and transcriptomic approaches. Plants were sampled during days of high (170 ppb) and moderate (87 ppb) O3 concentration. Given that there have been reforestation efforts in the region, with plants from different source populations, we first confirmed that all analyzed individuals clustered within the local genetic group when compared to a species‐wide panel (Admixture analysis with ~1.5K SNPs). We observed thicker epidermis and more collapsed cells in the palisade parenchyma of needles from symptomatic individuals than from their asymptomatic counterparts, with differences increasing with needle age. Furthermore, symptomatic individuals exhibited lower concentrations of various terpenes (ß‐pinene, ß‐caryophylene oxide, α‐caryophylene, and ß‐α‐cubebene) than asymptomatic trees, as evidenced through GC–MS. Finally, transcriptomic analyses revealed differential expression for 13 genes related to carbohydrate metabolism, plant defense, and gene regulation. Our results indicate a rapid and contrasting phenotypic response among trees, likely influenced by standing genetic variation and/or plastic mechanisms. They open the door to future evolutionary studies for understanding how O3 tolerance develops in urban environments, and how this knowledge could contribute to forest restoration.

European navigation in the age of sail owes much to the scientific revolution of the seventeenth century and the development of instruments and advanced mathematical techniques. Important though these developments were, it is argued here that close observation of the environment: of the weather, ocean currents, clouds, birds, mammals, and a host of other factors played a far more important role in safe navigation from one part of the globe to another.

When populations colonise new environments, they may be exposed to novel selection pressures but also suffer from extensive genetic drift due to founder effects, small population sizes and limited interpopulation gene flow. Genomic approaches enable us to study how these factors drive divergence, and disentangle neutral effects from differentiation at specific loci due to selection. Here, we investigate patterns of genetic diversity and divergence using whole‐genome resequencing (>22× coverage) in Berthelot's pipit ( Anthus berthelotii ), a passerine endemic to the islands of three north Atlantic archipelagos. Strong environmental gradients, including in pathogen pressure, across populations in the species range, make it an excellent system in which to explore traits important in adaptation and/or incipient speciation. First, we quantify how genomic divergence accumulates across the speciation continuum, that is, among Berthelot's pipit populations, between sub species across archipelagos, and between Berthelot's pipit and its mainland ancestor, the tawny pipit ( Anthus campestris ). Across these colonisation timeframes (2.1 million–ca. 8000 years ago), we identify highly differentiated loci within genomic islands of divergence and conclude that the observed distributions align with expectations for non‐neutral divergence. Characteristic signatures of selection are identified in loci associated with craniofacial/bone and eye development, metabolism and immune response between population comparisons. Interestingly, we find limited evidence for repeated divergence of the same loci across the colonisation range but do identify different loci putatively associated with the same biological traits in different populations, likely due to parallel adaptation. Incipient speciation across these island populations, in which founder effects and selective pressures are strong, may therefore be repeatedly associated with morphology, metabolism and immune defence.

Aim Wildlife overexploitation, either for food consumption or for the pet trade, is one of the main threats to bird species in tropical forests. Yet, the spatial distribution and intensity of harvesting pressure on tropical birds remain challenging to quantify. Here, we identify the drivers of hunting‐induced declines in bird abundance and quantify the magnitude and the spatial extent of avian defaunation at a pantropical scale. Location Pantropical. Methods We compiled 2968 abundance estimates in hunted and non‐hunted sites across the tropics spanning 518 bird species. Using a Bayesian modelling framework, we fitted species' abundance response ratios to a set of drivers of hunting pressure and species traits. Subsequently, we applied our model to quantify the spatial patterns of avian defaunation across tropical forests and to assess avian defaunation across biogeographic realms, and for species captured for the pet trade or for food consumption. Results Body mass and its interactions with hunter accessibility and proximity to urban markets were the most important drivers of hunting‐induced bird abundance declines. We estimated a mean abundance reduction of 12% across the tropics for all species, and that 43% of the extent of tropical forests harbour defaunated avian communities. Large‐bodied species and the Indomalayan realm displayed the greatest abundance declines. Further, moderate to high levels of defaunation extended over 24% of the pantropical forest area, with distinct spatial patterns for species captured for the pet trade (Brazil, China and Indonesia) and for food consumption (SE Asia and West Africa). Main Conclusions Our study emphasizes the role of hunter accessibility and the proximity to urban markets as major drivers of bird abundance declines due to hunting and trapping. We further identified hotspots where overexploitation has detrimental effects on tropical birds, encompassing local extinction events, thus underscoring the urgent need for conservation efforts to address unsustainable exploitation for both subsistence and trade.

Zoo populations of threatened species are a valuable resource for the restoration of wild populations. However, their small effective population size poses a risk to long‐term viability, especially in species with high genetic load. Recent bioinformatic developments can identify harmful genetic variants in genome data. Here, we advance this approach, analysing the genetic load in the threatened pink pigeon ( Nesoenas mayeri ). We lifted the mutation‐impact scores that had been calculated for the chicken ( Gallus gallus ) to estimate the genetic load in six pink pigeons. Additionally, we perform in silico crossings to predict the genetic load and realized load of potential offspring. We thus identify the optimal mate pairs that are theoretically expected to produce offspring with the least inbreeding depression. We use computer simulations to show how genomics‐informed conservation can reduce the genetic load whilst reducing the loss of genome‐wide diversity. Genomics‐informed management is likely to become instrumental in maintaining the long‐term viability of zoo populations.

The spontaneous formation of zonal jets is a distinctive feature of geostrophic turbulence with the phenomenon witnessed in numerous numerical studies. In such systems, strong rotation anisotropises the spectral evolution of the energy density such that zonal modes are favoured. In physical space, this manifests as eddies zonally elongating and forming into zonal jets. In the presence of large scale dissipation, the flow may reach statistical stationarity such that the zonal structure persists in the zonal and time mean, and is supported by a flux of eddy momentum. What is unclear is how the excitation of Rossby waves arranges the underlying eddy momentum stresses to support the mean flow structures. To study this, we examine a steady-state flow in the so-called ‘zonostrophic’ regime, in which characteristic scales of geostrophic turbulence are well separated and there are several alternating zonal jets that have formed spontaneously. We apply a geometric eddy ellipse formulation, in which momentum fluxes are cast as ellipses that encode information about the magnitude and direction of flux; the latter is described using the tilt angle. With the aid of a zonal filter, it is revealed that the scales responsible for providing the momentum fluxes associated with the jet structure are much smaller than the characteristic scales identified, and occupy a region of the energy spectrum that has been typically associated with isotropic dynamics.

Spatial navigation is a multi-faceted behaviour drawing on many different aspects of cognition. Visuospatial abilities, such as mental rotation and visuospatial working memory, in particular, may be key factors. A range of tests have been developed to assess visuospatial processing and memory, but how such tests relate to navigation ability remains unclear. This understanding is important to advance tests of navigation for disease monitoring in various disorders (e.g., Alzheimer’s disease) where spatial impairment is an early symptom. Here, we report the use of an established mobile gaming app, Sea Hero Quest (SHQ), as a measure of navigation ability in a sample of young, predominantly female university students (N = 78; 20; female = 74.3%; mean age = 20.33 years). We used three separate tests of navigation embedded in SHQ: wayfinding, path integration and spatial memory in a radial arm maze. In the same participants, we also collected measures of mental rotation (Mental Rotation Test), visuospatial processing (Design Organization Test) and visuospatial working memory (Digital Corsi). We found few strong correlations across our measures. Being good at wayfinding in a virtual navigation test does not mean an individual will also be good at path integration, have a superior memory in a radial arm maze, or rate themself as having a strong sense of direction. However, we observed that participants who were good in the wayfinding task of SHQ tended to perform well on the three visuospatial tasks examined here, and to also use a landmark strategy in the radial maze task. These findings help clarify the associations between different abilities involved in spatial navigation.

Globally, heatwaves have become more common with hazardous consequences on biological processes. Research using a model insect (Tribolium castaneum) found that 5-day experimental heatwave conditions damaged several aspects of male reproductive biology, while females remained unaffected. However, females’ reproductive fitness may still be impacted, as insects typically store sperm from multiple males in specialized organs for prolonged periods. Consequently, using males which produce sperm with green fluorescent protein (GFP)-tagged sperm nuclei, we visualized in vivo whether thermal stress affects the ejaculate occupancy across female storage sites under two scenarios; (i) increasing time since insemination and (ii) in the presence of defending competitor sperm. We reconfirmed that sperm from heatwave-exposed males sired fewer offspring with previously mated females and provided new scenarios for in vivo distributions of heat-stress-exposed males’ sperm. Sperm from heatwave-exposed males occupied a smaller area and were at lower densities across the females’ storage sites. Generally, sperm occupancy decreased with time since insemination, and sperm from the first male to mate dominated the long-term storage site. Reassuringly, although heated males’ ejaculate was less successful in occupying female tracts, they were not lost from female storage at a faster rate and were no worse than control males in their offensive ability to enter storage sites occupied by competitor sperm. Future work should consider the potential site-specificity of factors influencing sperm storage where amenable.

Introduction Demand for urgent and emergency health care in England has grown over the last decade, for reasons that are not clear. Changes in population demographics may be a cause. This study investigated associations between individuals’ characteristics (including socioeconomic deprivation and long term health conditions (LTC)) and the frequency of emergency department (ED) attendances, in the Norfolk and Waveney subregion of the East of England. Methods The study population was people who were registered with 91 of 106 Norfolk and Waveney general practices during one year from 1 April 2022 to 31 March 2023. Linked primary and secondary care and geographical data included each individual’s sociodemographic characteristics, and number of ED attendances during the same year and, for some individuals, LTCs and number of general practice (GP) appointments. Associations between these factors and ED attendances were estimated using Poisson regression models. Results 1,027,422 individuals were included of whom 57.4% had GP data on the presence or absence of LTC, and 43.1% had both LTC and general practitioner appointment data. In the total population ED attendances were more frequent in individuals aged under five years, (adjusted Incidence Rate Ratio (IRR) 1.25, 95% confidence interval 1.23 to 1.28) compared to 15–35 years); living in more socioeconomically deprived areas (IRR 0.61 (0.60 to 0.63)) for least deprived compared to most deprived,and living closer to the nearest ED. Among individuals with LTC data, each additional LTC was also associated with increased ED attendances (IRR 1.16 (1.15 to 1.16)). Among individuals with LTC and GP appointment data, each additional GP appointment was also associated with increased ED attendances (IRR 1.03 (1.026 to 1.027)). Conclusions In the Norfolk and Waveney population, ED attendance rates were higher for young children and individuals living in more deprived areas and closer to EDs. In individuals with LTC and GP appointment data, both factors were also associated with higher ED attendance.

The breakdown of plant material fuels soil functioning and biodiversity. Currently, process understanding of global decomposition patterns and the drivers of such patterns are hampered by the lack of coherent large‐scale datasets. We buried 36,000 individual litterbags (tea bags) worldwide and found an overall negative correlation between initial mass‐loss rates and stabilization factors of plant‐derived carbon, using the Tea Bag Index (TBI). The stabilization factor quantifies the degree to which easy‐to‐degrade components accumulate during early‐stage decomposition (e.g. by environmental limitations). However, agriculture and an interaction between moisture and temperature led to a decoupling between initial mass‐loss rates and stabilization, notably in colder locations. Using TBI improved mass‐loss estimates of natural litter compared to models that ignored stabilization. Ignoring the transformation of dead plant material to more recalcitrant substances during early‐stage decomposition, and the environmental control of this transformation, could overestimate carbon losses during early decomposition in carbon cycle models.