Trypan blue exclusion fraction versus electrical energy density. Variables were the electric field intensity which was varied between 150 kV/cm and 1 MV/cm, and the number of pulses which was varied between 1 and 20,000.

Contexts in source publication

Context 1

... data obtained with pulses of 800 ps duration seem to indicate that trypan blue uptake is a dose effect, scaling with the electrical energy density. Fig. 6 shows the trypan blue exclusion fraction (defined as: one minus uptake fraction) obtained 4 h after N pulses were applied with electric field amplitudes E, ranging from 150 kV/cm to 1 MV/cm, plotted versus the electric energy density, σE 2 τ N, where σ is the conductivity of the suspension (100 Ωcm). The exclusion of trypan blue ...

Context 2

... with various pulse parameters, but keeping the pulse duration constant, seem to indicate that the observed biological effect is an energy density-dependent effect (see Section III, Results). However, this dependency on energy density does not hold true when the pulse duration is varied. This is clearly shown when we compare the 800-ps data (Fig. 6) with trypan blue uptake data obtained with pulses 10 ns long [ Fig. 8(a)]. In Fig. 8(a) we have used experimental results on trypan blue exclusion reported in [24], complemented by results of a recent experimental study where the procedures were kept exactly the same as described in [24] (which are identical to those described in this ...