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SIRT1 expression in rhesus and marmoset monkey testes during different developmental stages. (a,b) Very few SIRT1-positive cells were observed in fetal and NB (1 month) rhesus monkey testes in the seminiferous tubules. (c,d) In the adult rhesus testis, all seminiferous tubule cross sections contained many SIRT1 positive germ cells. No staining was noted in control Immunoglobulin (IgG) antibody incubated testicular cross section of rhesus monkeys. (e,f) In NB (5 days) and juvenile (5-6 months) marmoset testes, SIRT1 positive cells were mainly observed in the tubular compartment. No staining was observed with control antibody (isotypic IgGs). (g,h) In the adult common marmoset testis SIRT1 was detected in germ and Sertoli cells. Scale bars, 50 µm.
Source publication
The epigenetic mechanisms controlling germ cell development and differentiation are still not well understood. Sirtuin-1 (SIRT1) is a nicotinamide adenosine dinucleotide (NAD)-dependent histone deacetylase and belongs to the sirtuin family of deacetylases. It catalyzes the removal of acetyl groups from a number of protein substrates. Some studies r...
Contexts in source publication
Context 1
... of the meiotic and postmeiotic germ cells were SIRT1 positive. SIRT1 expression was also observed on some selected pre-meiotic germ cells (Figure 2). SIRT1 signals in seminiferous tubules were observed in all developmental stages in both rhesus and marmoset monkeys testes sections. ...
Context 2
... of the meiotic and postmeiotic germ cells were SIRT1 positive. SIRT1 expression was also observed on some selected pre-meiotic germ cells (Figure 2). SIRT1 signals in seminiferous tubules were observed in all developmental stages in both rhesus and marmoset monkeys testes sections. ...
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... the nonbreeding season with low spermatogenic activity, the expression of SIRT1 was high. However, when spermatogenesis is active during the breeding season, expression of SIRT1 is low and numbers of SIRT1-positive cells are low (Supplementary Figure S2). These findings show a negative correlation of spermatogenesis and SIRT1 expression. ...
Citations
... SIRT1 activates peroxisome proliferator-activated receptor gamma co-activator 1 alpha (PGC-1α) and estrogenrelated receptor alpha (ERRα) which is also known as a nuclear receptor 1 (Wan et al. 2022). Moreover, SIRT1 interacts with various molecular pathways including hairy enhancer of spilt related with YRPW motif protein 2 (HEY2), autoimmune regulator (AIRE), miR-132, and poly ADP-ribose polymerase 1 (PARP-1) (Wahab et al. 2021). As well, SIRT1 deactivates the p53 protein preventing apoptosis and cell deaths (Khanahmadi et al. 2020). ...
Silent information regulator (SIRT) has distinctive enzymatic activities and physiological functions to control cell-cycle progression, gene expression, and DNA stability by targeting histone and non-histone proteins. SIRT1 enhances synaptic formation and synaptic activity, and therefore, can reduce the progression of various degenerative brain diseases including Parkinson’s disease (PD). SIRT1 activity is decreased by aging with a subsequent increased risk for the development of degenerative brain diseases. Inhibition of SIRT1 promotes inflammatory reactions since SIRT1 inhibits transcription of nuclear factor kappa B (NF-κB) which also inhibits SIRT1 activation via activation of microRNA and miR-34a which reduce NAD synthesis. SIRT1 is highly expressed in microglia as well as neurons, and has antioxidant and anti-inflammatory effects. Therefore, this review aimed to find the possible role of SIRT1 in PD neuropathology. SIRT1 has neuroprotective effects; therefore, downregulation of SIRT1 during aging promotes p53 expression and may increase the vulnerability of neuronal cell deaths. PD neuropathology is linked with the sequence of inflammatory changes and the release of pro-inflammatory cytokines due to the activation of inflammatory signaling pathways. In addition, oxidative stress, inflammatory disorders, mitochondrial dysfunction, and apoptosis contribute mutually to PD neuropathology. Thus, SIRT1 and SIRT1 activators play a crucial role in the mitigation of PD neuropathology through the amelioration of oxidative stress, inflammatory disorders, mitochondrial dysfunction, apoptosis, and inflammatory signaling pathways.
... Hence, there is a need for additional studies in the intratesticular characterisation of this deacethylase. The research article by Wahab et al. [24] promptly analyses the expression of Sirt1 in the testes of primates and non-primate species during the annual reproductive cycle, revealing high Sirt1 protein levels in germ cells at mitotic, meiotic and post-meiotic phase. Interestingly, for the first time in primates, colocalisation of Sirt1 with the myokine irisin in primate spermatogonia and the irisin-dependent expression of Sirt1 mRNA have been reported in primate spermatogonia. ...
Infertility is currently one of the most important health troubles in industrialised countries after cardio-vascular diseases and cancer [...]
... Their role in reproduction has recently attracted attention with most studies focusing on SIRT1, mainly in female reproduction (Loganathan et al., 2021;Tatone et al., 2018). On the other hand, there is evidence supporting that SIRT1 is implicated in spermatogenesis as well by influencing functions of germ cells, Sertoli and Leydig cells (Loganathan et al., 2021;Tatone et al., 2018;Wahab et al., 2021). Nevertheless, the exact role of sirtuin members in male reproduction is still obscured (Loganathan et al., 2021) and ...
AbstractMitochondrial sirtuins (SIRT3, SIRT4, SIRT5) are post- translational modifiers that regulate energy production, body homeostasis and mitochondrial activities via dif-ferent substrates in response to environmental stressors. The present study aimed at assessing the expression of SIRT3, SIRT4, and SIRT5 in the semen of infertile men. Expression analysis was performed using q- RT PCR. All mitochondrial sirtuin genes were significantly down- regulated in the semen of infertile men compared to fertile men. Mitochondrial sirtuin genes expression levels were correlated with mitochon-drial HSP90 expression. HSP90 expression was positively correlated with SIRT3, S I R T4 and SIRT5 expression in the semen of fertile men, while a negative correlation was observed between HSP90 in the semen of infertile men and mitochondrial sirtuin genes in the semen of fertile men. These data suggest that dysregulation of mito-chondrial sirtuin genes causes mitochondrial dysfunction due to stress, which ap-pears to be associated with human male infertility by compromising functional and structural sperm integrity.KEYWORDShuman, infertility, male, mitochondria, semen, sirtuins
Sirt1 is a member of the Sirtuins family that regulates ovarian senescence, follicular development, and oocyte maturation in vertebrates. To understand its role in the ovary of Pelodiscus sinensis, we cloned the full-length cDNA of Ps-Sirt1 and characterized its potential function by intraperitoneally injecting agonist (resveratrol) and antagonist (EX527) in the female juvenile turtle. The full-length cDNA of Ps-Sirt1 was 2106 bp, comprising 203 bp 5'UTR, a 226 bp 3'UTR, and a 1677 bp ORF encoding 558 amino acids. The calculated molecular weight of predicted protein was 63 kDa, and the isoelectric point was 4.65. The predicted protein comprised a conserved Sir2 domain. Amino acid sequence alignment and phylogenetic analyses showed that Ps-Sirt1 was most closely related to turtles, and distantly related to fish. Expression pattern analysis showed Ps-Sirt1 was highest expressed in ovary, followed by testis, liver, heart, and brain. In the ovarian differentiation processes, Sirt1 showed significantly higher expression at embryonic stage 15 and 21. In the testis differentiation process, Sirt1 expression was downregulated at embryonic stages 15-19. Activated and inactivated Sirt1 decreased the number of primordial follicles in juvenile turtles. Bcl2, Bax, mTOR, and rpS6 expressions were up-regulated, whereas GnRH, Fshb, p50, and p65 were down-regulated after agonist treatment. The inaction of Sirt1 with antagonist up-regulated GnRH, Fshb, p65, p53, Foxo3a, Bcl2, Bax, mTOR, and rpS6, but down-regulated p50. In summary, Sirt1 might be involved in the ovarian follicle development of P. sinensis.
