Figure 4
Representative histograms showing the activation of caspase-8. H1299 cells (10 5 ) were seeded in 24-well plate and after 24 hrs were exposed to CS (200 and 400µΜ) for another 24 hrs. A significant increase in activated caspase-8 was observed after treatment with CS.
Source publication
Purpose:
Lung cancer is among the leading causes of cancer-related cases and cancer-associated deaths. Tumor cells frequently acquire chemoresistance and, due to that, new therapies are always needed in the fight against cancer. Pharmaceutical plants continue to offer novel compounds as anticancer therapies.
Methods:
We studied the action of N-p...
Context in source publication
Context 1
... we investigated the effect of CS in caspase-8 activation, after treatment with 200 and 400µM of CS. While at 200 µΜ there was no significant caspase 8 induction, at 400 µΜ CS produced significant higher activity of caspase-8 compared to control, indicating induction of apoptosis (Figure 4). ...
Citations
... 97 Moreover, p-coumaroyl serotonin has been observed to demonstrate antiproliferative activity in lung and breast cancer cells. 70,98 Mitsis and colleagues determined that p-coumaroyl serotonin can reduce cell viability, arrest cell cycle at the S phase, and induce apoptosis by activation of caspase-8 and decrease in CD15, CD24, CD44, CD56, CD58, and CD71 expressions in the MCF7 breast cancer cell line, 98 while CD15 and CD71 expressions were increased in U251MG and T98G glioblastoma cell lines. 97 In the H1299 lung cancer cell line, they also found p-coumaroyl serotonin can reduce cell viability, cause S-phase cell cycle arrest, and induce apoptosis by activation of caspase-8 and decrease of CD24, CD44, CD58, and CD71 expressions. ...
... 97 In the H1299 lung cancer cell line, they also found p-coumaroyl serotonin can reduce cell viability, cause S-phase cell cycle arrest, and induce apoptosis by activation of caspase-8 and decrease of CD24, CD44, CD58, and CD71 expressions. 70 The tyramine-derived phenolamides also have been observed to possess anticancer abilities. 15,99 p-Coumaroyl tyramine exhibited an ability to inhibit the growth of U937 and Jurkat cells, and the suppressed growth of the cells was strongly associated with an increased percentage of the cells being arrested in the S phase of the cell cycle progression. ...
Phenolamides, also known as hydroxycinnamic acid amides or phenylamides, have been reported throughout the plant kingdom, while a few of these amine-conjugated hydroxycinnamic acids are unique in foods. The current knowledge on their specific functions in plant development and defense is readily available as well as their biosynthesis; however, their functionality in humans is still unknown. Of the currently known phenolamides, the most common are avenanthramides, which are unique in oats and similar with the well-known drug, Tranilast, that possess anti-inflammatory, antioxidant, anti-itch, and anti-atherogenic activities. While recent data has brought to light more information regarding the other known phenolamides, such as hordatines, dimers of agmatine conjugated hydroxycinnamic acid, and kukoamines, spermine-derived phenolamides, the information is still severely limited, leaving their potential health benefits to speculation. Herein, to highlight the importance of dietary phenolamides to human health, we review and summarize the four major subgroups of phenolamides, including their chemical structures, dietary sources, and reported health benefits. We believe that the studies on phenolamides are still in the infancy stage and additional health benefits, of these phenolamides, may have yet to be identified.
