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Three simple, sensitive, precise and accurate UV-spectroscopic methods namely simultaneous equation, absorbance ratio and first derivative (zero crossing) spectroscopic methods were developed and validated for simultaneous determination of aliskiren hemifumarate and hydrochlorothiazide in tablet dosage form. Simultaneous equation method was based o...
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... AR method 255 and 271 nm was used for the detection and quantification of ALI and HCT. 1 st DR method was involved in the conversion of UV-spectra in to first derivative spectra and measurement of first derivative signal at 241 & 280.20 nm for ALI and HCT, respectively using 2 nm as wavelength interval (Δλ) and 1 as scaling factor. Comparative overlain spectra of placebo and drug solutions indicate that there was no interference between excipients and standard drugs (Figure 4 & 5). ALI and HCT were found to be linear in the concentration range of 6-300 µg/ml and 0.5-25 µg/ml, respectively for all the methods. ...
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A simple, rapid, and precise reverse phase high performance liquid chromatographic (RP-HPLC) method for simultaneous analysis of Nebivolol and Hydrochlorothiazide in a tablet dosage form and in Biorelevant media has been developed and validated by utilizing Zodiac silC18 portion (4.60×150mm) 05µ, stream rate was 1ml/min, adaptable stage degree was...
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In the present analytical study a rapid, robust and specific UPLC method has been developed and validated for Aliskiren hemifumarate and Hydrochlorothiazide in bulk and in combined tablet dosage form and it was applied to the dissolution study of Aliskiren hemifumarate and Hydrochlorthiazide. The separation was carried out on acquality UPLC HSS T3...
The chromatographic conditions were successfully developed for the separation of Hydrochlorothiazide and Telmisartan by
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Citations
... Outcomes were subjected to regression study using the technique of least squares to set up slope, intercept, and correlation coefficient values. [37][38][39] ...
... The established methodologies were validated as given in the recommendations of the "International Conference on Harmonization. " 12,37,38,42,[47][48][49] Specificity A specificity study was performed to examine the interaction between tablet formulation excipients and drug ingredients. All tablet excipients (according to the commercial formulation) were mixed ratio wise, diluted with methanol, and passed through a whatman filter paper no 41. ...
Background and Aim: Tablets containing celecoxib and tramadol hydrochloride that are sold
under the brand name Seglentis® have given approval by the “Food and Drug Administration”
(FDA) for the treatment of adults suffering from acute pain. Simultaneous assessment of
celecoxib and tramadol hydrochloride in synthetic mixture was performed by five new UV
spectrophotometric approaches. Materials and Methods: The quantification of proposed
medication was performed using the simultaneous equation approach by measuring absorbance
at 217.2 and 272.4 nm. The second approach, known as the dual wavelength method, relies on
computing the absorbance difference at two different wavelengths where the other drug has
same absorbance. The first derivative zero crossing approach relied on the conversion of UV
spectra into first derivative spectra and subsequently measuring the amplitude at 272.4 and
229.6 nm for celecoxib and tramadol hydrochloride, respectively. The ratio difference approach
was used to measure the variation in the amplitudes of ratio spectra, and the regression equation
was employed to determine the amounts of pharmaceuticals. In first derivative ratio spectra
approach, the UV spectra were transformed into their ratio spectra and first derivatives, and the
first-derivative signal was gauged at 236.4 and 229.8 nm for celecoxib and tramadol hydrochloride,
sequentially. Results and Discussion: All the parameters were evaluated during validation of
the new methods in accordance with ICH guidelines. For both medicines, a linear response was
observed in all five methods over the concentration series of 1-18 µg/mL. Method validation
parameters were found to be within the permitted limits set forth by the ICH. Conclusion: Assay
results were compared using Repeated Measures ANOVA, IBM SPSS, version 20.0. The findings of
the statistical analysis demonstrated that none of the projected methods differed significantly
from others. Thus, the anticipated approaches have the potential to be productively utilized for
the simultaneous evaluation of celecoxib and tramadol hydrochloride in a combination mixture.
Keywords: Celecoxib, Tramadol hydrochloride, Simultaneous equation, Dual wavelength, First
derivative zero crossing, Ratio difference and the First derivative of ratio spectra spectroscopic
methods, Binary mixture
... Both the solutions were scanned in the UV region (200 -400 nm) and spectra were recorded. Based on the spectral pattern, SE (simultaneous equation) and AR (absorbance ratio) methods [Beckett and Stenlake, 2005] were chosen for the estimation of both the drugs. From the overlain spectra ( Figure 2), 237 nm (λ max of MET) and 246 nm (λ max of TEN) were selected for SE method. ...
... After scanning the sample solution (formulation) between 200 to 400 nm, responses were noted at 237 and 246 nm. The unknown concentration of drugs present in the sample solution was estimated by solving following formula (Sen et al., 2016): ...
Three new UV spectrophotometric methods namely simultaneous equation, absorbance ratio and first derivative (zero crossing) spectroscopic methods were developed and validated for simultaneous estimation of teneligliptin hydrobromide hydrate and metformin hydrochloride in tablet formulation which were simple, sensitive, precise and accurate. In simultaneous equation method, absorbance was measured at 237 and 246 nm for both the drugs. Teneligliptin hydrobromide hydrate and metformin hydrochloride was estimated using 237 and 247.5 nm in absorbance ratio method. First derivative (zero crossing) method was based on the transformation of UV spectra in to first derivative spectra followed by measurement of first derivative signal at 237 and 246 nm for teneligliptin hydrobromide hydrate and metformin hydrochloride, respectively using 2 nm as wavelength interval (Δλ) and 1 as scaling factor. Developed methods were validated according to ICH guidelines including parameters viz., specificity, linearity and range, precision, accuracy, limit of detection and quantification. All the three methods showed linear response in the concentration range of 1-20 μg/ml for both the drugs. Results of method validation parameters follows ICH guideline acceptable limits. Based on the assay results obtained, methods were compared using one-way ANOVA followed by Bonferroni multiple comparison tests (95% confidence level) using computer based fitting program (Prism, Graphpad version 5, Graphpad Software Inc). Outcome of the statistical analysis proved that there was no considerable dissimilarity between all the developed methods. Methods were found to be simple, fast, highly sensitive, cost effective and hence can be useful for simultaneous estimation of teneligliptin hydrobromide hydrate and metformin hydrochloride in commercial tablet formulation for routine quality control analysis.
In the study simultaneous assessment of teneligliptin hydrobromide hydrate and metformin hydrochloride in tablets were developed and validated by four new UV spectroscopic approaches. The ratio difference approach was used to measure the variation in two selected amplitudes of ratio spectra, and the regression equation was employed to determine the amounts of pharmaceuticals. The second approach, known as the first derivative of ratio spectra approach, the UV spectra were transformed into their ratio spectra and corresponding first derivatives, and the first-derivative signal was gauged at 275 and 226.2 nm for metformin hydrochloride and teneligliptin hydrobromide hydrate, sequentially, making use of 2 nm as the wavelength interval (??) and a factor of 1 as a scaling factor, respectively. Whereas absorbance subtraction method works with the principle of finding isosbestic point and calculating absorption factor. The fourth approach, known as the dual wavelength method, relies on computing the absorbance difference at two different wavelengths where the other drug has same absorbance. Linearity and range, specificity, accuracy, precision, limit of detection and quantification were among the criteria evaluated during validation of the new methods in agreement with ICH recommendations and found to be within the permitted limits. For both the medicines, a linear response was observed in all four methods over the concentration sequence of 2-12 µg/ml. Therefore, the proposed methodologies may be successfully used for the concurrent evaluation of teneligliptin hydrobromide hydrate and metformin hydrochloride in tablet form.
In this paper, we report a novel electrochemical sensor for the sensitive detection of hydrochlorothiazide based on La ³⁺ /ZnO nanoflower supported on graphite screen printed Electrode. Firstly we reported the synthesis and characterization of La ³⁺ /ZnO nanoflower. The as-synthesized nanostructures. La ³⁺ /ZnO nanoflower was employed to modify the screen printed electrode and demonstrated its excellent electrocatalytic activities towards hydrochlorothiazide (HCT). Voltammetric measurements were utilized to assess the electrochemical properties towards the hydrochlorothiazide detection. The resulting sensor exhibits excellent sensitivity. Finally, this modified electrode was effectively applied to determine hydrochlorothiazide in real samples with good recoveries.
