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Functional annotations of differentially expressed genes in HFD-fed animals compared to control animals.
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The most prevalent chronic liver disorder in the world is fatty liver disease caused by a high-fat diet. We examined the effects of Lactiplantibacillus plantarum-KCC48 on high-fat diet-induced (HFD) fatty liver disease in mice. We used the transcriptome tool to perform a systematic evaluation of hepatic mRNA transcripts changes in high-fat diet (HF...
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... animals fed with HFLPD were found to have 62 differentially expressed genes (p < 0.05) in the liver tissue compared to animals fed with HFD. There were 36 genes (58.06%) upregulated and 26 genes (41.94%) downregulated (Figure 1d,f and see the Supplementary Table S2). The differentially expressed genes in HFD liver were mainly enriched in the GO terms extracellular matrix (1.17%), aging (0.34%), angiogenesis (1.29%), and neurogenesis (0.47%), followed by immune and inflammatory responses, cellular migration, cell differentiation, cell death, and apoptotic processes ( Figure 2a). ...Context 2
... animals fed with HFLPD were found to have 62 differentially expressed genes (p < 0.05) in the liver tissue compared to animals fed with HFD. There were 36 genes (58.06%) upregulated and 26 genes (41.94%) downregulated (Figure 1d,f and see the Supplementary Table S2). The differentially expressed genes in HFD liver were mainly enriched in the GO terms extracellular matrix (1.17%), aging (0.34%), angiogenesis (1.29%), and neurogenesis (0.47%), followed by immune and inflammatory responses, cellular migration, cell differentiation, cell death, and apoptotic processes ( Figure 2a Gene categorization of identified DEGs in the liver of HFD-fed mice compared to control mice (p < 0.05, greater than 2-fold); (b) Gene categorization of identified DEGs in the liver of HFLPD-fed mice compared to HFD (p < 0.05, greater than 2-fold); (c) distribution of identified DEGs in the liver of experimental animals. ...Context 3
... than ten counts were used to detect functional characterizations. DEGs in HFD were associated with more than 40 biological functions, including steroid hormone biosynthesis; cholesterol, lipid, and cholesterol metabolism; endoplasmic reticulum; cytochrome p450; and disulfide bond ( Table 2). The DEGs identified in HFLPD liver tissues are closely associated with 28 biological functions, including membrane, metal binding, endoplasmic reticulum, disulfide bond, glycoprotein, oxidoreductase activity, metabolic pathways, retinol metabolism, cytochrome p450, and monooxygenase (Table 3). ...Citations
... In silage production from grasses, legumes, and other plants, lactic acid bacteria (LAB) play a critical role. Since ensiling is a consistent, L. plantarum-KCC-34 [20], L. plantarum-KCC-48 [21], P. pentosaceus-KCC-44 [22], P. pentosaceus-KCC-53 (GenBank accession No. MZ505239), and L. rhamnosus-KCC-54 (GenBank accession No. MZ505240) strains were isolated from different sources and have been reported previously. All the strains were cultured in MRS broth (CONDA, Madrid, Spain) for 30 h with mild shaking at 150 rpm in an orbital shaker under micro-aerobic conditions at 37 • C, and the pellets were removed by centrifugation at 4000× g for 45 min at 4 • C. ...
Lactic acid bacteria (LAB) are excellent anaerobic fermenters that produce highly valuable grass-based animal feed containing essential nutrients. In the present study, an ensiling process was used to improve anaerobic fermentation in triticale silage under different moisture conditions with LAB. The triticale was treated with either a single bacterium or combined LAB and then vacuum-sealed. After 180 and 360 days of storage, the silage’s fermentation characteristics, microbial changes and nutrient contents were analyzed. The pH of the silage was significantly lower than the control silage. There was a significant difference in the pH values between the silages treated with single or mixed LAB. The LAB treatment led to a substantial increase in lactic acid (LA), a decrease in butyric acid (BA), and marginal levels of acetic acid (AA). The LA content after the mixed LAB treatment was significantly higher than that after the single culture LAB treatment. After single or combined inoculant treatments, the LAB population in the silage increased, while the yeast and mold levels decreased. These findings suggest that the addition of LAB to silage during ensiling could enhance the nutritional quality and reduce unwanted microbial growth. The mixed LAB treatments produced silage with a significantly higher nutritional value than the single LAB treatments.
Fatty liver syndrome (FLS) is a kind of nutritional metabolic disease in laying hens. Revealing FLS pathogenesis during the early period is what really makes sense for the prevention or nutritional regulation strategies. In the study, 9 healthy or naturally occurring early FLS birds were screened based on visual inspection, liver index and morphologic analysis. Liver and fresh cecal content samples were collected. Then transcriptomic and 16S rRNA technologies are applied to investigate hepatic transcriptome and cecum microbiota composition. Unpaired Student t test and some omics methods were used for statistical analysis. Results showed higher liver weight and index were found in FLS group; morphologic analysis indicated that there existed more lipid droplets in the liver of birds with FLS. Based on DESeq2 analysis, there were 229 up- and 487 down-regulated genes in the FLS group, among which most genes related to de novo fatty acid synthesis were up-regulated such as acetyl-CoA carboxylase, fatty acid synthase, stearoyl-CoA desaturase, and ELOVL fatty acid elongase 6 (ELOVL6). Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis indicated that pathways associated with lipid metabolism and liver damage were affected. 16S rRNA sequencing analysis of cecum microbiota showed that there was a significant difference between the Con and FLS groups. LEfSe analysis revealed that the relative abundance of Coprococcus, Odoribacter, Collinsella, Turicibacter, YRC22, Enterococcus, Shigella, and Bifidobacterium were down-regulated in the FLS group, whereas the abundance of Bacteroides, Mucispirillum, Butyricicoccus, Campylobacter, Akkermansia, and Clostridium were up-regulated. The KEGG enrichment from differential microbiota suggested that some metabolism-related functions were altered to some extent. Taken together, during the developmental of early fatty liver of laying hens, lipogenesis was enhanced, whereas abnormal metabolism occurs not only in lipid transportation but also in hydrolysis, which caused structural damage to the liver organ. Moreover, the dysbiosis of the cecum microbiota occurred. All of these serve as targets or provide theoretical references for the development of probiotics for fatty liver prevention in laying hens.
