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DNA cleavage activities of 1. Lane 1: Control pBR 322 DNA. Lane 2: pBR 322 DNA + 25 μg of 1. Lane 3: pBR 322 DNA + 50 μg of 1. Lane 4: pBR 322 DNA + 75 μg of 1. Lane 5: pBR 322 DNA + 100 μg of 1.
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![Figure 1. An ORTEP diagram of [Zn 3 L 2 (μ-O 2 CCH 3 ) 2 (CH 3 OH) 4 ]...](profile/Dhananjay-Dey-2/publication/263393218/figure/fig13/AS:667111626125323@1536063214894/An-ORTEP-diagram-of-Zn-3-L-2-m-O-2-CCH-3-2-CH-3-OH-4-1-with-atom-numbering_Q320.jpg)
A novel trinuclear zinc(II) complex [Zn3L2(μ-O2CCH3)2(CH3OH)4] (1) that contains an N,O-donor Schiff base ligand {H2L = 2-[(2-hydroxyphenylimino)methyl]-6-methoxyphenol} has been synthesized and crystallographically characterized. The X-ray crystal structure of 1 contains three zinc(II) centers, which have distorted-octahedral coordination geometry...
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... formation of the respective quinone derivative, 3,5-dtbq, was purified by column chromatography using a hexane/ethyl acetate eluant mix- ture. The product was isolated by slow evaporation of the eluant and was identified by H 1 NMR spectroscopy (Fig- ure S4 in the Supporting Information). H 1 NMR (CDCl 3 , 300 MHz): δ H = 1.22 (s, 9 H), 1.26 (s, 9 H), 6.21 (d, J = 2.13 Hz, 1 H), 6.92 (d, J = 2.10 Hz, 1 H) ppm. ...
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... ability of the zinc(II)-Schiff base complex to cleave DNA was assayed with the aid of gel electrophoresis on pBR 322 DNA as the substrate in a medium of 5 mm tris- (hydroxymethyl)aminomethane (Tris)-HCl/50 mm NaCl buffer (pH 7.1) in the absence of external additives. The DNA was mixed with different concentrations (25, 50, 75, and 100 μg) of zinc(II)-Schiff base complex and was incu- bated at 37 °C for 1 h (Figure 4). Lane 1 in Figure 4 shows the pBR 322 DNA control. ...
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... DNA was mixed with different concentrations (25, 50, 75, and 100 μg) of zinc(II)-Schiff base complex and was incu- bated at 37 °C for 1 h (Figure 4). Lane 1 in Figure 4 shows the pBR 322 DNA control. Lane 2 in Figure 4 shows the DNA treated with 25 μg of the zinc(II)-Schiff base com- plex. ...
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... 1 in Figure 4 shows the pBR 322 DNA control. Lane 2 in Figure 4 shows the DNA treated with 25 μg of the zinc(II)-Schiff base com- plex. There was a complete cleavage of the DNA with the addition of 25 μg of the complex. ...
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... was a complete cleavage of the DNA with the addition of 25 μg of the complex. Further addition of dif- ferent concentrations (50, 75, and 100 μg) of the complex in lane 3, lane 4, and lane 5 of Figure 4 did not show bands for the DNA. This finding clearly suggests that the concen- tration of the complex needed to cleave the pBR 322 DNA was 25 μg. ...
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Background:
Pyridinium Schiff bases and ionic liquids have attracted increasing interest in medicinal chemistry.
Results:
A library of 32 cationic fluorinated pyridinium hydrazone-based amphiphiles tethering fluorinated counteranions was synthesized by alkylation of 4-fluoropyridine hydrazone with various long alkyl iodide exploiting lead quater...
Citations
... Antimicrobial active cadmium Schiff base complexes synthesized from substituted amine and aryl aldehyde derivatives were reported by Irfan and co-workers [57]. The Schiff base ligand derivatives were prepared via condensation reaction of amantadine 109 with aryl aldehyde 108 such as, salicylaldehyde, 3-ethoxysalicylaldehyde and 4-(diethylamino) salicylaldehyde are good candidates to obtained the target compound 110. ...
... Scheme 15 General synthesis of Schiff base and its zinc complex derivative[56] Scheme 16 General synthesis of Schiff bases and their cadmium complex derivatives[57] ...
Schiff bases transition metal complexes have received significant attentions in the scientific community for their versatile applications. The incorporation of metals to Schiff base ligands attracts much attention, since the metals and Schiff base ligands coordinated via bonding. Thus, chelation effects will enhance and improve the biological activities of the derivatives of title compound. Most of these derivatives displayed broad range bioactivities including antibacterial, antifungal, antituberclosis, antimalarial, antioxidant, antidiabetic, anti-inflammatory and anticancer activities. Up to date, continuous efforts are being made in various research groups to design the outcomes of more potent, novel and safe synthetic protocols towards the Schiff base metal complexes. Therefore, this review summarizes the various synthetic protocols and pharmacological activities of Schiff base metal complexes and their derivatives. Here, the synthesis of Schiff base ligands and their complexes with metals Cu(II), Pt(II), Ni(II), Pd(II), Ru(II, III), V(III), Cd (II), Zn(II), Co(II, III) and Mn(II, III) will be presented
... Homoleptic and heteroleptic Schiff base metal complexes.Schiff Base in Organic, Inorganic and Physical Chemistry Mononuclear and polynuclear Schiff base metal complexes[56][57][58][59][60][61]. ...
The development of Schiff base was a major step forward in the area of coordination chemistry. Schiff bases, a class of organic compounds, carry the imine or azomethine (>C=N–) functional group. Schiff bases played an influencing role in the development of coordination chemistry and were a key point in the development of inorganic, bioinorganic chemistry and optical materials. Schiff bases, widely used in inorganic, organic, and analytical chemistry, account for a significant portion of the more commonly employed classes of organic molecules. The ability of Schiff base ligands to form stable metal complexes with a wide range of transition and other metal ions makes them extremely useful. Condensation of a primary amine with an aldehyde or ketone yields a Schiff bases. In this chapter, we focused on introducing Schiff bases, classified them and their metal complexes, and discussed several synthesis methods, including conventional and green approaches. This chapter also elaborated on the industries’ applications, such as the food industry, agrochemical industry, dye industry, analytical chemistry, catalysis, energy storage, environmental, chemo-sensing, bio-sensing, and biomedical applications of novel Schiff bases and their metal complexes.
... 5 Schiff base ligands via imine nitrogencontaining compounds have broad aspects of biological applications, viz., anti-inflammatory 6 , analgesic 7 , antitubercular 8 , antimicrobial 9 , antioxidant 10,11 , anticonvulsant 12 , and anticancer 13 . The influence of metal ions in complexes is essential to be addressed to expose their efficacy 14 as the redox characteristics of those compounds are connected to the pharmacological activities. 15 Since the metal ions' and ligands' characteristics affect the pharmacological efficacy of metal complexes 16,17 , it's crucial to expose and compare their pharmacological efficiencies. ...
... Å in five-membered metallocycle and 1.315-1.350 Å in six-membered metallocycles [36][37][38][39][40][41][42]. The C-N distances in the earlier reported complexes are 1.280-1.300 ...
A number of novel heteroligand Zn(II) complexes (1–8) of the general type (Ln)Zn(NN) containing O,N,O′-, O,N,S-donor redox-active Schiff bases and neutral N,N′-chelating ligands (NN) were synthesized. The target Schiff bases LnH2 were obtained as a result of the condensation of 3,5-di-tert-butyl-2-hydroxybenzaldehyde with substituted o-aminophenols or o-aminothiophenol. These ligands with combination with 2,2′-bipyridine, 1,10-phenanthroline, and neocuproine are able to form stable complexes upon coordination with zinc(II) ion. The molecular structures of complexes 4∙H2O, 6, and 8 in crystal state were determined by means of single-crystal X-ray analysis. In the prepared complexes, the redox-active Schiff bases are in the form of doubly deprotonated dianions and act as chelating tridentate ligands. Complexes 6 and 8 possess a strongly distorted pentacoordinate geometry while 4∙H2O is hexacoordinate and contains water molecule coordinated to the central zinc atom. The electrochemical properties of zinc(II) complexes were studied by the cyclic voltammetry. For the studied complexes, O,N,O′- or O,N,S-donor Schiff base ligands are predominantly involved in electrochemical transformations in the anodic region, while the N,N′-coordinated neutral nitrogen donor ligands demonstrate the electrochemical activity in the cathode potential range. A feature of complexes 5 and 8 with sterically hindered tert-butyl groups is the possibility of the formation of relatively stable monocation and monoanion forms under electrochemical conditions. The values of the energy gap between the boundary redox orbitals were determined by electrochemical and spectral methods. The parameters obtained in the first case vary from 1.97 to 2.42 eV, while the optical bang gap reaches 2.87 eV.
... This band displayed a bathochromic shift after complexation and was identified in the range 228-240 nm. The second band owing to the n-π* [49,50] transition due to the -C=N group shift to a longer wavelength in complexes and absorbs in the range 348-366 nm. The third band is attributed to the charge transfer within the furan moiety. ...
Condensation of 5-(hydroxymethyl)furan-2-carboxaldehyde with 4-toluidine yielded a Schiff base (5-((p-tolylimino)methyl)furan-2-ol). A series of seven novel hexa-coordinated organyltellurium(IV) complexes of type TeCl4·HMeFPT, RTeCl3·HMeFPT and R2TeCl2·HMeFPT (where R belongs to 4-methoxyphenyl, 4-hydroxyphenyl and 3-methyl-4-hydroxyphenyl; L belongs to HMeFPT i.e. Schiff base have been prepared and characterized by molar conductance, IR, UV, 1H NMR, 13C NMR, mass spectroscopy and elemental analysis. Thus according to spectroscopic studies, Schiff base ligand functions as a NO donor bidentate ligand across all organyltellurium(IV) complexes via the azomethine nitrogen atom and the oxygen atom of the furan ring. The above mentioned spectroscopic analysis indicated that all the organyltellurium(IV) complexes have distorted octahedral geometry. Schiff base and all the organyltellurium(IV) complexes had their geometry optimized and theoretical quantum mechanical characteristics computed. The octahedral geometry for complexes is also suggested by this computing analysis. The antimicrobial property of the Schiff base ligand and its organyltellurium(IV) complexes was evaluated in vitro against different bacterial strains viz. A. clavatus, C. albicans, A. niger, S. pyogenes, S. aureus, E. coli and P. aerugenosa. Antimicrobial property of heterocyclic bidentate Schiff base and its organyltellurium(IV) complexes was found to be significantly greater than that of some standard antibiotics.
... Prepared SCs were treated with 20 mL·well −1 of MTT reagent (20 mL·well −1 ) and incubated further for 4 hours at 37°C after a 24-hour incubation period. e purple formazan product was dissolved in all of the wells by adding 100 mL of DMSO solvent [26]. Using a plate reader, the aforementioned solution absorption was measured at 570 nm (iMark, Bio-Rad, USA). ...
The impact of a soluble complex (SC) of curcumin (CuR) synthesized using hot melt (HM) and hot-melt extrusion (HE) technologies on adenocarcinoma cells for the treatment of colorectal cancer by enhancing CuR solubility is investigated in this work. In silico molecular modelling, solubility, drug release, and physicochemical analysis were all part of the phase solubility (PS) study, which featured a novel dyeing test and a central composite design to optimize the best complex (CDD). The optimal HE-SC (1 : 5) enhances solubility (0.8521 ± 0.016 mg·mL−1) and dissolution (91.87 ± 0.208% at 30 min), and it has an ideal stability constant (309 and 377 M−1) at 25 and 37°C and an AL type of isotherm, implying 1 : 1 stoichiometry according to the findings. An intermolecular hydrogen bond that has not undergone any chemical change and has resulted in the complete conversion of the amorphous form aids in the creation of SC. In vitro cytotoxicity was measured at IC50 on the SW480 (72 M·mL−1) and Caco-2 (40 M·mL−1) cells. According to apoptotic studies, apoptosis was responsible for the vast majority of cell death, with necrosis accounting for a small proportion of the total. In vivo toxicity was established using a zebrafish model, and a western blot examination revealed apoptosis at the molecular level. It was argued that the novel formulations developed using HE technology are more significant and effective than existing pure CuR formulations.
... After the cells have been counted, a cell suspension containing 5000 cells is added to each well of a 96-well plate, with the exception of the first column, which is left blank. The plate was incubate using CO 2 incubator for 24 hours at 37°C in a humidified CO 2 environment of 5% [21]. ...
The core objectives of the research were to prepare 5-fluorouracil nanoemulsion (FU-NE) and to evaluate the physiochemical properties and to study the in vitro antiproliferation in HepG2 cell lines. The physiochemical parameters determined were compatibility, particle size (PS), polydispersity index (PDI), zeta potential (ZP), density, surface tension (ST), pH, viscosity, in vitro release of FU, cytotoxicity, and apoptosis study. The prepared FU-NE3 was stable, sterile, and homogeneous. On the HepG2 (120 μg.mL-1) cells, in vitro cytotoxicity was obtained at IC50 concentration. Apoptosis examination by AO/EBand Hoechst staining shows that the majority of cell demise was caused by apoptosis, with a tiny fraction of necrosis. Hence, this investigation concluded that the developed FU-NE has now desirable characteristics for drug delivery to the cancer cell and may be screened for the in vivo colorectal anticancer activity.
... Apoptotic morphology investigation was carried out by using acridine orange and ethidium bromide (AO/EB) twin staining method [51]. Briefly, determined IC50 concentration of the SC complex was treated with SW480 and Caco-2 cells (cell passage number was 8) and incubated for 24 h followed by washed with ice-cold phosphate-buffered saline (PBS). ...
This study was designed to investigate the effects of curcumin (CMN) soluble complex (SC) prepared by melt casting (HM) and hot-melt extrusion (HME) technology. Phase solubility (PS) study, in silico molecular modeling, aqueous solubility, drug release, and physicochemical investigation including a novel dyeing test was performed to obtain an optimized complex by a central composite design (CCD). The results show that the HME-SC produces better improvements towards solubility (0.852 ± 0.02), dissolution (91.87 ± 0.21 % at 30 min), with an ideal stability constant (309 and 377 M −1 at 25 and 37 °C, respectively) and exhibits AL type of isotherm indicating 1:1 stoi-chiometry. Intermolecular hydrogen bonding involves the formation of SC, which does not undergo any chemical modification, followed by the complete conversion of the amorphous form which was identified by XRD. The in vitro cytotoxicity showed that IC50 was achieved in the SW480 (72 µM.mL −1) and Caco-2 (40 µM.mL −1) cells while that of pure CMN ranged from 146 to 116 µM/mL −1. Apoptosis studies showed that cell death is primarily due to apoptosis, with a low rate of necrosis. In vivo toxicity, confirmed by the zebrafish model, exhibited the safety of the HME-SC. In conclusion , the HME-SC potentially enhances the solubility and cytotoxicity to the treatment of colorectal cancer (CRC).
... The absorption features of the mixture in the range of the wavelength 300-800 nm at the interval of 8 min for 2h confirm the oxidation of 3,5-DTBC. Kinetic experiments were also carried out spectrophotometrically following the reported procedures [13][14][15] . 0.04 mL of 1×10 -4 M 1 was added to 2 mL of 3,5-DTBC of a particular concentration (varying its concentration from 1×10 -3 M to 1×10 -2 M) to attain the final concentration of 1 as 1×10 -4 M. The dependence of rate on the concentration of 3,5-DTBC was determined by kinetic analyses and all the measurements were performed in triplicate. ...
A trans-diaqua nickel(II) complex of the type [L2-)NiII(H2O)2].nH2O (1.nH2O) was isolated where LH2 is (E)-2-(2-((2-hydroxyphenylimino)methyl)phenoxy)acetic acid (LH2), a tetradentate ligand. The molecular geometry of 1.nH2O was confirmed by single crystal X-ray structure determination. It is observed that in the crystal, coordinated water, bulk water and ligand oxygen atoms form six membered water clusters by OH••••H interaction. 1.nH2O has been emerged as a catalyst for the oxidation of 3,5-di-tert-butylcatecholto 3,5-di-tert-butyl-o-benzoquinone with turnover number (kcat) as 4.46102 h-1 in CH3OH. During oxidation, the coordination of catechol to the nickel(II) centre and the formation of a o-benzosemiquinone intermediate were confirmed by a nickel based EPR signal, ESI mass spectrometry and UV-Vis spectra. 1.nH2O exhibits an irreversible anodic peak at 0.83 V versus Fc+/Fc couple due to phenoxyl/phenolato redox couple, authenticated by DFT calculations.
... MBAP was prepared using a procedure reported in the literature 35 by refluxing O-aminophenol (0.1092 g, 1 mol) with O-vanillin (0.1523 g, 1 mmol) in 20 ml dehydrated alcohol. After 10 h, the reaction solution was kept in an open atmosphere and a red colored crystalline compound was separated from the solution, which was dried over CaCl 2 and stored in vacuum for subsequent use. ...
Multiple molecular logic gates were harvested on a single synthesized material, (E)-2-(2-hydroxy-3-methoxybenzylideneamino)phenol (MBAP), by combining excitation wavelength dependent multi-channel fluorescence outputs and the same chemical inputs. Interestingly, the effortless switching of logic behavior was achieved by simply tweaking the excitation wavelength and sometimes the emission wavelengths with no alteration of chemical inputs and the main device molecule, MBAP. Additionally, new generation purely optically driven memory units were designed on the same system supporting an almost infinite number of write–erase cycles since inter-conversion of memory states was completely free from chemical interferences and impurity issues. Two-way memory functions (“erase–read–write–read” and “write–read–erase–read”) worked simultaneously on the same system and could be accessed by simple optical switching between two excitation and emission wavelengths. Our optically switchable device might outperform traditional multifunctional logic gates and memory devices that generally employ chemical triggers to switch functionality and memory states. These optically switchable multifunctional molecular logic gates and memory systems might drive smart devices in the near future with high energy efficiency, extended life span, structural and functional simplicity, exclusive reversibility and enhanced data storage density.
