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(A) Effects of MMC treatment on the growth of Halomonas sp. H4907. In the prophage induction experiment, one subculture (solid square) was treated with MMC, while the other subculture (hollow square) served as a control. Bacterium growth was detected at OD 600 , and the time points at which MMC was added are indicated by an arrow. The data shown are average values from triplicate experiments, and error bars indicate standard deviations. (B) TEM morphology of Halomonas phage vB_HmeY_H4907; the scale bar is 100 nm.

(A) Effects of MMC treatment on the growth of Halomonas sp. H4907. In the prophage induction experiment, one subculture (solid square) was treated with MMC, while the other subculture (hollow square) served as a control. Bacterium growth was detected at OD 600 , and the time points at which MMC was added are indicated by an arrow. The data shown are average values from triplicate experiments, and error bars indicate standard deviations. (B) TEM morphology of Halomonas phage vB_HmeY_H4907; the scale bar is 100 nm.

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FIG 1 (A) Effects of MMC treatment on the growth of Halomonas sp....
FIG 2 Genome map of the genome sequence of Halomonas phage...
FIG 3 (A) Phylogenetic tree of all isolated Halomonas phages by...
FIG 4 (A) Shared gene heat map of Halomonas phage vB_HmeY_H4907 and the...
FIG 5 Relative abundances of Halomonas phage vB_HmeY_H4907 compared to...
Identification and genomic analysis of temperate Halomonas bacteriophage vB_HmeY_H4907 from the surface sediment of the Mariana Trench at a depth of 8,900 m
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  • Sep 2023
Viruses play crucial roles in the ecosystem by modulating the host community structure, mediating biogeochemical cycles, and compensating for the metabolism of host cells. Mariana Trench, the world’s deepest hadal habitat, harbors a variety of unique microorganisms that have adapted to its extreme conditions of low temperatures, high pressure, and...
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... sp. H4907 was isolated from 8,900-m-deep sediment in the Mariana Trench of the Western Pacific. The 16S rRNA sequence of the host strain was most similar to that of Halomonas meridiana (Fig. S1). To examine whether H4907 contained inducible temperate phage, mitomycin C (MMC) (1 µg/mL) was added to H4907 cultures at the early stage of the exponential growth period (24 h, OD 600 = 0.5). We found that the growth of H4907 was significantly inhibited by the addition of MMC, suggesting that bacterial lysis induced by prophage may ...
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... To examine whether H4907 contained inducible temperate phage, mitomycin C (MMC) (1 µg/mL) was added to H4907 cultures at the early stage of the exponential growth period (24 h, OD 600 = 0.5). We found that the growth of H4907 was significantly inhibited by the addition of MMC, suggesting that bacterial lysis induced by prophage may have occurred (Fig. 1A). Unlike the untreated group, the host bacterial suspension became clear after incubation with Halomonas phage vB_HmeY_H4907 (about 96 h), which indicated that the bacteria were killed by the phage (Fig. S2). TEM reveals the siphoviral morphology of Halomonas phage vB_HmeY_H4907, which has an icosahedron head (average diameter: 65 nm) ...
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... suspension became clear after incubation with Halomonas phage vB_HmeY_H4907 (about 96 h), which indicated that the bacteria were killed by the phage (Fig. S2). TEM reveals the siphoviral morphology of Halomonas phage vB_HmeY_H4907, which has an icosahedron head (average diameter: 65 nm) and a long, non-contractible tail (average length: 183 nm) (Fig. 1B). The phage vB_HmeY_H4907 was a temperate phage induced by MMC, and the integrase was also present on the genome, but it did not show obvious phage plaques. However, there was a clear clarification of the host bacterial suspension in the liquid infection, so we hypothesized that there might be a phage re-infection of the host ...
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... following material is available online. Fig. S1 (Spectrum01912-23-s0001.tif). Phylogenetic tree of Halomonas H4907 and 50 reference sequences of Halomonas. Fig. S2 (Spectrum01912-23-s0002.tif). The host strain was incubated with phage vB_HmeY_H4907 suspension for 96 h. Fig. S3 (Spectrum01912-23-s0003.tif). Cumulative GC skew analysis of the phage genome sequence. Fig. S4 (Spectrum01912-23-s0004.tif). Genomic ...
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... following material is available online. Fig. S1 (Spectrum01912-23-s0001.tif). Phylogenetic tree of Halomonas H4907 and 50 reference sequences of Halomonas. Fig. S2 (Spectrum01912-23-s0002.tif). The host strain was incubated with phage vB_HmeY_H4907 suspension for 96 h. Fig. S3 (Spectrum01912-23-s0003.tif). Cumulative GC skew analysis of the phage genome sequence. Fig. S4 (Spectrum01912-23-s0004.tif). Genomic comparisons between Halomonas phage vB_HmeY_H4907 and 14 related viruses. S5 (Spectrum01912-23-s0005.tif). Gene map of ...
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... following material is available online. Fig. S1 (Spectrum01912-23-s0001.tif). Phylogenetic tree of Halomonas H4907 and 50 reference sequences of Halomonas. Fig. S2 (Spectrum01912-23-s0002.tif). The host strain was incubated with phage vB_HmeY_H4907 suspension for 96 h. Fig. S3 (Spectrum01912-23-s0003.tif). Cumulative GC skew analysis of the phage genome sequence. Fig. S4 (Spectrum01912-23-s0004.tif). Genomic comparisons between Halomonas phage vB_HmeY_H4907 and 14 related viruses. S5 (Spectrum01912-23-s0005.tif). Gene map of Halomonas phage vB_HmeY_H4907 and 14 related viruses. Fig. S6 (Spectrum01912-23-s0006.tif). Protein cluster ...
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... is available online. Fig. S1 (Spectrum01912-23-s0001.tif). Phylogenetic tree of Halomonas H4907 and 50 reference sequences of Halomonas. Fig. S2 (Spectrum01912-23-s0002.tif). The host strain was incubated with phage vB_HmeY_H4907 suspension for 96 h. Fig. S3 (Spectrum01912-23-s0003.tif). Cumulative GC skew analysis of the phage genome sequence. Fig. S4 (Spectrum01912-23-s0004.tif). Genomic comparisons between Halomonas phage vB_HmeY_H4907 and 14 related viruses. S5 (Spectrum01912-23-s0005.tif). Gene map of Halomonas phage vB_HmeY_H4907 and 14 related viruses. Fig. S6 (Spectrum01912-23-s0006.tif). Protein cluster analysis between Halomonas phage vB_HmeY_H4907 and 14 vB_HmeY_H4907-associated genomes. Table S1 ...
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... comparisons between Halomonas phage vB_HmeY_H4907 and 14 related viruses. S5 (Spectrum01912-23-s0005.tif). Gene map of Halomonas phage vB_HmeY_H4907 and 14 related viruses. ...
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... suspension for 96 h. Fig. S3 (Spectrum01912-23-s0003.tif). Cumulative GC skew analysis of the phage genome sequence. Fig. S4 (Spectrum01912-23-s0004.tif). Genomic comparisons between Halomonas phage vB_HmeY_H4907 and 14 related viruses. S5 (Spectrum01912-23-s0005.tif). Gene map of Halomonas phage vB_HmeY_H4907 and 14 related viruses. Fig. S6 (Spectrum01912-23-s0006.tif). Protein cluster analysis between Halomonas phage vB_HmeY_H4907 and 14 vB_HmeY_H4907-associated genomes. Table S1 (Spectrum01912-23-s0007.docx). Genome annotation of Halomonas phage vB_HmeY_H4907. Table S2 (Spectrum01912-23-s0008.docx). HHperd hits of Halomonas phage vB_HmeY_H4907. Table S3 (Spectrum01912-23-s0009.docx). Information of ...
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... cluster analysis between Halomonas phage vB_HmeY_H4907 and 14 vB_HmeY_H4907-associated genomes. Table S1 (Spectrum01912-23-s0007.docx). Genome annotation of Halomonas phage vB_HmeY_H4907. ...
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... annotation of Halomonas phage vB_HmeY_H4907. Table S2 (Spectrum01912-23-s0008.docx). HHperd hits of Halomonas phage vB_HmeY_H4907. ...
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... hits of Halomonas phage vB_HmeY_H4907. Table S3 (Spectrum01912-23-s0009.docx). Information of Yueviridae virus in the IMG/VR database. ...
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... of Yueviridae virus in the IMG/VR database. Table S4 (Spectrum01912-23-s0010.docx). Information of provirus in Yueviridae by CheckV predicted in the host sequence associated with Halomonas phage vB_HmeY_H4907. ...