Mohammad HaddadiUniversity of Zabol · Department of Biology
Mohammad Haddadi
Ph.D in Genetics
About
39
Publications
100,230
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Introduction
Additional affiliations
October 2014 - present
January 2013 - present
September 2009 - April 2014
Education
September 2009
Publications
Publications (39)
Alzheimer's disease (AD), a prevalent global cause of dementia lacking a cure, has traditionally been studied from a molecular perspective. However, our investigation delved into the intricate cellular dimensions of AD, focusing on microglial cells, the primary immune cells in the central nervous system (CNS). Through a single-cell approach using s...
Background:
Identifying mutual neuroinflammatory axis in different experimental models of multiple sclerosis (MS) is essential to evaluate the de- and re-myelination processes and improve therapeutic interventions' reproducibility.
Methods:
The expression profile data set of EAE (GSE47900) and cuprizone (GSE100663) models were downloaded from th...
Alzheimer's disease (AD) is the most common form of tauopathy that usually occurs during aging and unfolded protein response (UPR), oxidative stress and autophagy play a crucial role in tauopathy-induced neurotoxicity. The aim of this study was to investigate the effects of tauopathy on normal brain aging in a Drosophila model of AD. We investigate...
Honey bees may experience the adverse effects of environmental contaminants when exposed during foraging trips, or something to that effect. We hypothesized that octopamine (OA), with important functions in reward signaling and learning, can improve the behavior of honey bees under chemical stress. Memory retention and sucrose responsiveness were e...
The role of nutrition in alleviating the physiological disorders caused by toxicants in honeybees is currently receiving considerable attention. This study aimed to determine whether dietary supplementation with whey protein (WP) could protect Apis mellifera from manganese and paraquat-induced disorders. In this study, honeybees were exposed to sub...
Background
Tau, Amyloid-beta (Aβ42), and Glycogen synthase kinase 3 (GSK3) contribute to synaptic dysfunction observed in Alzheimer's disease (AD), the most common form of dementia. In the current study, the effect of pan-neuronal expression of TauWT, Aβ42, or shaggy (orthologue of GSK3) in Drosophila melanogaster was assessed on the locomotor func...
Multiple sclerosis (MS) is an autoimmune disease that affects the central nervous system. Although remarkable progress has been made in treating MS, current therapies are less effective in protecting against the progression of the disease. Since cucurbitacins have shown an extreme range of pharmacological properties, in this study, we aimed to inve...
Background: Tau, Amyloid-beta (Aβ42), and Glycogen synthase kinase 3 (GSK3) contribute to synaptic dysfunction observed in Alzheimer's disease (AD), the most common form of dementia. In the current study, the effect of pan-neuronal expression of TauWT, Aβ42, or shaggy (orthologue of GSK3) in Drosophila melanogaster was assessed on the locomotor fun...
Intellectual disability (ID) is a heterogeneous disorder with high prevalence and remarkable social and cost burdens. Novel genetic variants of ATF7IP, CAPN9, ITGAV, ITSN1, and UBR4 genes are reported to be associated with the ID among Iranian families. However, in vivo validation is required to confirm the functional role of these variants in ID d...
Background
Hypoxia-inducible factor-1α (HIF-1α) and its downstream gene, vascular endothelial factor (VEGF), play an important role in modulating the immune system function and consequently alternation the susceptibility to systemic lupus erythematosus (SLE) disease.
Objective
The present study aimed to investigate the effect of the HIF-1α and VEG...
Parkinson’s disease (PD) is an age-associated neurodegenerative condition in which some genetic variants are known to increase disease susceptibility on interaction with environmental factors inducing oxidative stress. Different mutations in the SNCA gene are reported as the major genetic contributors to PD. E46K mutation pathogenicity has not been...
Introduction: Methamphetamine (MA) acts as a powerful oxidant agent, while Rosmarinic Acid (RA) is an effective herbal antioxidant. Oxidative stress-mediated by MA results in apoptosis, and caspase-3 is one of the critical enzymes in the apoptosis process. MA can epigenetically alter gene regulation. In this paper, to investigate the effects of RA...
Two major pathological hallmarks of AD are amyloid plaques and neurofibrillary tangles of hyperphosphorylated tau. Aggregation of amyloid beta is considered as the primary insult in AD. However, failure in treatments based on targeting amyloid-β (Aβ) without considering the pathologic tau and close correlation between pathological tau and cognitive...
Alzheimer’s disease (AD) is one of the most common forms of neurodegenerative diseases. Aggregation of Aβ42 and hyperphosphorylated tau are two major hallmarks of AD. Whether different forms of tau (soluble or hyperphosphorylated) or Aβ are the main culprit in the events observed in AD is still under investigation. Here, we examined the effect of w...
Parkinson’s disease (PD) is a class of neurodegenerative disorders in which, complex interactions of genetic and environmental agents are involved in the etiology of both sporadic and familial PD cases. α-synuclein- encoding SNCA gene is known as one of the major genetic contributors of this disease. E46K mutation in SNCA gene has not been investig...
Iran, despite its size, geographic location and past cultural influence, has largely been a blind spot for human population genetic studies. With only sparse genetic information on the Iranian population available, we pursued its genome-wide and geographic characterization based on 1021 samples from eleven ethnic groups. We show that Iranians, whil...
Introduction: Repeated heat shock (HS) stresses reduce the reproduction rate of Drosophila flies. Heat shock proteins (HSPs) protect cells against irreversible damages inducing heatinduced.Oxidative stress declines protective function of HSPs. Diploschistes ocellatus lichen aqueous extract possesses a strong antioxidant potential in vitro. Antioxid...
The advent of high‐throughput sequencing technologies has led to an exponential increase in the identification of novel disease‐causing genes in highly heterogeneous diseases. A novel frameshift mutation in CNKSR1 gene was detected by Next‐Generation Sequencing (NGS) in an Iranian family with syndromic autosomal recessive intellectual disability (A...
Exploring genes and pathways underlying Intellectual Disability (ID) provides insight into brain development and function, clarifying the complex puzzle of how cognition develops. As part of ongoing systematic studies to identify candidate ID genes, linkage analysis and next generation sequencing revealed ZBTB11, as a novel candidate ID gene. ZBTB1...
The central nervous system (CNS) is the most complex part of the human body, which controls a variety of cellular and molecular activities. Neurobehavioral functions of CNS play a vital role in making appropriate responses to the environmental stimuli. Some kind of such responses can be maintained in neural networks due to neuronal plasticity. When...
Oxidative stress is one of the major etiological factors implicated in pathogenesis of neurodegenerative diseases. Since neurons are more sensitive to oxidative damage there is an increasing interest in developing novel antioxidant therapies, especially herbal preparations due to their safety profile and high efficiency. In this regard, the neuropr...
Mutations in the human microtubule-associated protein tau (hMAPT) gene including R406W and V337M result in autosomal dominant neurodegenerative disorder. These mutations lead to hyperphosphorylation and aggregation of Tau protein which is a known genetic factor underlying development of Alzheimer's disease (AD). In the present study, transgenic Dro...
Oxidative stress is believed to be a major factor for the onset of Parkinson's disease (PD). In this study, we have investigated oxidative status in transgenic Drosophila model of PD. Our results revealed elevated levels of reactive oxygen species (ROS) and lipid peroxidation (LPO) in A30P and A53T α-synuclein PD model flies compared to control. We...
Oxidative stress induces alterations in the structure and functions of cellular macromolecules that result in many pathological conditions including the aging process. According to the free radical theory of aging, oxidative stress is implicated in the age-related functional decline. Brain is more vulnerable to free radical damages due to its highe...
In this paper, we have demonstrated for the first time, the antioxidant and neuroprotective effects of Decalepis hamiltonii (Dh) root extract against paraquat (PQ)-induced oxidative stress and neurotoxicity in Drosophila melanogaster. Exposure of adult D. melanogaster (Oregon K) to PQ induced oxidative stress as evidenced by glutathione depletion,...
Memory impairment during aging is believed to be a consequence of decline in neuronal function and increase in neurodegeneration. Accumulation of oxidative damage and reduction of antioxidant defense system play a key role in organismal aging and functional senescence. In our study, we examined the age-related memory impairment (AMI) in relation to...
Age-associated accumulation of oxidative damage linked to decline of antioxidant defense mechanism, leads to impairment of cognitive function in many organisms. These damages can pass through generations and affect the cognitive quality of progenies. In Drosophila, classical olfactory conditioning results in the formation of different types of memo...
Questions
Questions (18)
Instinct responsive behaviors are very stable and can last for hundreds of generations. For example in fruit flies (Drosophila melanogaster) flies that were kept in a vivarium for 80 years and never been exposed to a predator (i . e. Wasps) exhibit special reaction in terms of egg laying capacity.
How this kind of behavior exists for many many generations in lab-grown animals?
I will be glad if you can share your thoughts with me.
I look for a robust and simple protocol for Golgi staining of Drosophila CNS. Please share your experience in this matter with me.
Dear Researchers,
I am going to make a list of genes with unknown function in Drosophila melanogaster. Can anyone help me out?
I work on making taupathy Drosophila models. I usually set a cross between male flies of UAS-Tau and virgin females of elav-Gal4. I may had 5-10 culture vials with around 10 female and 20 male flies in each. Then I take the F1 offspring and select a mixed population of 100 flies, dissect their head and use as samples for RNA extraction and cDNA synthesis. Once cDNA is prepared with desired quality, then 3 independent qRT PCR tube will be ran for each experimental or control group. The obtained results are statistically analyzed to see possible changes in the expression level of any target gene,. Is this method correct?
Dear all colleagues. I need to do antibody staining for mushroom bodies in the fly brain. Samples were fixed in 4% paraformaldehyde (20 min), washed, and blocked with BSA in PBX. I used anti FasII antibody (1D4) at 1:20 dilution for 48 hours at 4-degree celsius. Then washing and incubation with secondary antibody (anti-mouse IgG Alexa 488 coupled) at 1:300 dilution for 2 hours at room temperature. Finally, samples were washed, mounted in vectashield medium and scanned under confocal microscope. We got weird results (an image is attached). Can anyone suggest me some points on it?
Thanks
Hello o everyone
There are orders of numbers within a bar beneath each individual of a pedigree representing genetic linkage. Those numbers are corresponding to specific DNA markers. For example 4, 3, 3, 2. Its is marked with a red circle in the attached figure. I need to know its meaning and interpretation.
There are many feasible approaches in functional analysis of genes using Drosophila as a model including Gal4-UAS system. In this category what could be the best way to neurodegenerative disorders?
I have established a new zebrafish lab. At present I face with the problem of fish mating and reproduction. Though the female fishes are mature enough and receive enough food they are not laying eggs. Is there any suggestion in this regard? I am really in trouble. Please help me out. Thanks
There is a huge difference between DNA content of each chromosome. Beside, each eukaryotic organism has specific number of chromosomes. I need to know the possible cellular and molecular bases for such these differences.
I need to know if there is any online calculator or software to calculate chi square, odds ratio and 95% CI in additive genetic model of a case control study . If not, which type of chi square test should be applied to additive genetic model in a case control study?
Although Bi-PASA is an old method, but in some extend we can use it even nowadays. I need to have knowledge about the points and tips which are needed for primer design. I will be glad if you can suggest me any e-resource, research method papers or books.
