plant pathogens - Science topic
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Questions related to plant pathogens
I am looking for the protocol(s) to evaluate the antifungal properties of some PGPRs against soil-borne plant pathogens in lab conditions. Kindly guide me. Thank you.
Are plant pathogens belonging to same strain affecting both humans and animals?
I was wondering for how much time does a mycelium freshly isolated from plant tissue continue to be "similar to what it used to be" in nature. I can see that morphological changes frequently occur when transferring from plate to plate so in short, my question is: can I keep using a mycelium for a long time, making sure it still is the same? Should I re-isolate it from time to time? And, are there some techniques to prevent changes to happen?
I found a genus of fungi, which was previously defined by some scholars as not pathogenic bacteria, but the samples I collected recently are all in line with plant pathogenic bacteria. Does anyone know how to identify plant pathogenic bacteria or recommend related literatures
Normally in bacteria, both sexual and asexual reproduction occurs. In the case of Plant pathogenic bacteria, I only get the mention of binary fission and reproduction by means of spore in some bacteria. Even in the book of G.N Agrios, there is mention of only fission and spores as a means of reproduction. Isn't there a mention of sexual reproduction like conjugation, transduction, etc in Plant Pathogenic Bacteria? Need Help.
Peracetic acid (also known as peroxyacetic acid, or PAA) is registered antimicrobial in USA (1985) and European Union (2013). It was used for post-harvest plant treatment (Abd-Alla MA, Abd-El-Kader MM, Abd-El-Kareem F, El-Mohamedy RS. Evaluation of lemongrass, thyme and peracetic acid against gray mold of strawberry fruits. Journal of Agricultural Technology. 2011;7(6):1775-87.)
It is recommended for plant treatment (0.15% solution) against a wide range of pathogens. But, is there any confirmed trials of antiviral, antibacterial or fungicidal properties in field or greenhouse application of PAA?
Okra seedlings were raised.
Pathogen inoculation was done during sowing.
This was followed by biological control.
Unfortunately, we came across tragedy in our personal apple garden and a few of our trees have majorly infected by Fire Blight pathogenic agent. I have attached a few photos of infected trees, I think there is a relationship between the white stains on the bark of the tree and diseases outbreak. I think the more white spots there are, the more the tree is exposed to the disease. However, this is my experimental understanding and I have no academic expertise in this field. That disease has appeared only on the red apple trees.
I would be very happy if you put me in the right pass and let me know how I can overcome that disease.
I've been using Qiagen DNeasy Plant Pro kits to extract plant pathogen DNA from diseased plant material. Unfortunately the Lysis Buffer (Solution CD1) has been spilt whilst there are still 137 tests left (arrgh!). Currently replacement Plant Pro kits are hard to come by, so I am trying to find out where I might be able to obtain some replacement solution CD1 or possibly a recipe for a lysis buffer that will work with the Plant Pro kit.
I observed the attached symptoms on Eucalyptus.
Do you have any idea what causes these symptoms?
When I tried to remove it, in was hard to be removed. It looks like tumors.
Thanks in advance,
steril control of a mineral substrats for plants
grow medium: sabouraud agar
sterile control of a mineral substrate for plants
grow medium: sabouraud agar
Spray-induced gene silencing, SIGS, is tested for plant diseases control. Is there any results on dsRNA formulation trials that showed long life for dsRNA in a product and an efficient transport into plants/pathogens/pests?
Can the hypersensitive response occur as part of PAMP-Triggered Immunity (PTI) as well as Effector-Triggered Immunity (ETI)?
Are resistance (R) proteins (encoded by R genes) involved in pathogen Avr protein recognition/interaction only? Or do genes associated with plant immune signalling or other pathogen defence mechanisms also fall into the category of R gene? For example pathogeneis related (PR) protein encoding genes or genes involved in salicylic acid signalling?
A biofilm comprises any syntrophic consortium of microorganisms in which cells stick to each other and often also to a surface. These adherent cells become embedded within a slimy extracellular matrix that is composed of extracellular polymeric substances (EPSs). The cells within the biofilm produce the EPS components, which are typically a polymeric conglomeration of extracellular polysaccharides, proteins, lipids and DNA. Because they have a three-dimensional structure and represent a community lifestyle for microorganisms, they have been metaphorically described as "cities for microbes".
Greenhouses and nurseries are particularly vulnerable to biofilm, a complex structure adhering to surfaces that are regularly in contact with water, consisting of colonies of bacteria that secrete a protective coating in which they are encased. Light is not necessary to create a biofilm. It is easily created in nutrient-rich environments such as a greenhouse irrigation system, where an ample supply of fertilizer-injected water assures an ample food supply to the bacteria.
Biofilm is created very quickly and needs to be continuously treated. If not treated, your irrigation system becomes a distribution point for detached biofilm clogging irrigation lines and emitters, which leads to extra labour, crop shrink, and irrigation equipment costs.
In this case, this farmer from Moguer (Huelva, Spain) had a serious problem with the creation of biofilm in his irrigation system, clogging the drippers and leaving several hectares without being irrigated, generating serious losses in such an expensive crop as raspberries.
Changing pipes and irrigation systems for new ones worked for a while, but they quickly went back to the same old ones. And the cost of personnel to replace these irrigation systems, and the material costs, made this crop unviable.
What would you do to solve this problem? (watch video)
About 1/4 of field was affected by white heads symptoms. What can it be? Temperature range was high: 5 (night) - 30oC (day) within 4 last weeks.
Best methods suitable for isolation and purification of plant pathogenic fungi and bacteria from plant and soil samples....Latest methods....Any special advantages....Procedure to be followed...etc.
Are pathogenesis-related proteins and antimicrobial peptides encoded by plant resistance genes (R genes)?
I'm unsure if the genes for these proteins/peptides are types of R genes?
I work with the oospores (average diameter 27.7 µm) of a plant-pathogenic oomycete. In searching for antagonists of them, I incubate them with enzymes in solution or co-culture them with other microbes. I then assess viability changes using a combination of dyes - fluorescein diacetate and DITO-3 iodide.
My problem is that I always end up with a large amount of residual enzyme (and even buffer) or microbial residue in the tubes at the time of staining, which leads to many unexpected effects on the dyes (fluorescein diacetate in particular) and skewed results. I wash with sterile ultra-pure water and centrifugation twice before staining. I have tried adding more washes but it did not help and significantly increased the duration of the assay as well as increasing oospore loss (need to maintain oospore numbers for counting later on).
I have been thinking about filtration set-ups that might allow me to catch anything above, say, 15 µm and let through everything else. But I work with 24+ 1.5 ml tubes for each experiment, so the apparatus would need to work with this in mind and all the micro-centrifuge filtration options I can see are the typical 0.22/0.45 µm pore size (and would be a wasteful and expensive option anyway). Another limiting factor is the need to easily resuspend the oospores that I catch, in good numbers, to be stained. And this is a medium-throughput assay with small volumes, which also limits options.
I have also thought about flow cytometry/FACS, but the machines I have access to for these are on the other side of campus and would add an hr+ to an already long pipeline, where all I want is to reduce signal noise from residual enzymes. This option may be of more use down the line, when microbe-microbe interactions are a bigger focus.
Does anyone have any experience with this sort of very specific scenario? Or any ideas for how to more effectively wash my oospores clean before staining?
Bio char is a type of charcoal used as a soil ameliorant for both carbon sequestration and soil health benefits and also being used for the management of soil borne plant pathogens but I doubt whether it will be an economic and ecofriendly approach for the management of plant diseases.
I need to understand most simple, and widely used method to study antagonistic activity of biocontrol bacteria against plant pathogenic bacteria.
Does anyone know if there are recent study about plant pathogenic fungi or invasive fungi surveillance? Such as sudden oak death or other important fungi.
The climate change is thought to affect the virulence of some known plant pathogens and can also lead to the emergence of new virulent strains. I want to know if the otherwise is possible?
Hi, Can plant pathogens cause disease and epidemics in humans? Do you have an example?
I have different fungus isolates such as;
1) Fusarium kyushuense , 2) Fusarium proliferatum ;3) Aspergillus aculeatus ;4) Aspergillus europaeus ; 5) Aspergillus flavus ; 6) Penicillium chermesinum ; 7) Microdochium phragmitis ;, 8) Cladosporium tenuissimum ;9) Colletotrichum truncatum ;10) Fusarium concentricum ; 11) Fusarium equiseti ; 12) Alternaria alternata
I want to do the pathogenicity testing, so
1: how old is the "fungal isolate" most suitable for pathogenicity testing on the surface of fruits?
2: which method is best for pathogenicity testing a) conidiospore suspension 1*10^6 /ml or b) through plugs
Electronic nose uses digital sensors which mimic human nose to identify a particular odour. In what way it finds application in plant pathology. If it can detect odour caused by rotting of a plant part due to a disease how the electronic nose is able to distinguish between odours caused by different plant pathogens
I have Penicillium isolates had highest antagonistic activities against the mycelial growth of plant pathogens in the dual culture technique test.
I am looking for help with any study on optimum using to kinds alcohol in extraction the bioactive compounds from the Penicillium sp.
with my great thanks
I am working on proteomics, and I am looking for a plant pathogen updated database. Most of them are no longer available.
Thank you so much!
I was collected my ten scale level data from from infected and control(non inoculated) by plant pathogen. Therefore, which statistical tools is appropriate for my data analysis?
As part of my PhD program I would require some Verticillium dahliae for inoculations on olive plantlets, preferably VD pathotype D of known virulence.
Is there anyone working with this pathogen willing to share their isolates or perhaps to share an information on where to obtain them?
Would appreciate any information you can provide.
I have seen different protocols abt leaf infection to deal with pathogenicity and control of plant pathogens especially when work focus on studying metabolomic and/ phenomics
Yet what are the performing critirea using only a leaf in the in vivo study especially when we know that there is more than the plant component that play the role in triggering pathogenicity and concluding/making decision in disease management strategies ?
please, attached articles if possible.
I am working on fungi associated with crown rot of banana so I need a book to refer to for identification.
We isolate many species of Trichoderma .molecular identification revealed some of them are T.longibrachitum ,which exhibited high antagonistic activity against some plant pathogenic fungi,however we want to know if this species have been used as bio-agent.
It is difficult to work on crops and chances of successful experiments are very less, that is why we prefer these model organisms to study the mechanism and then apply the same knowledge on other crops. So, according to you, for different traits and developmental studies which is better?
What your opinion fora about partial and complete resistance to plant pathogens?
What your opinion for about tolerance?
Describe to us your concepts and uses?
Pseudomonas corrugata causes pith necrosis of tomato, diseases of chrysantemium, geranium plants, isolated from roots of other crops, and applyed as biocontrol method against plant pathogenic fungi. Do you know any reports about Pseudomonas corrugata pathogenic for cucurbits?
We already know that some Legumes are producing substances like thioglucosides and/ or isothiocyanates that kill plant pathogens in the soil. There must be other examples. Have you a recent reference. Thank you
I plan to assess the effect of compost tea on a specific soil-borne plant pathogen using soil bioassays. The general plan I have in mind is to sterilize the soil, inoculate it with the pathogen, and incubate it for a period of time. I will then apply compost tea on the inoculated soil.
Can someone suggest papers and/or methods on which I can base my methods on?
There is a lot of papers on the use of compost tea to suppress disease in pot based settings. However, I have yet to read papers which focuses only on the inhibition of pathogen growth in soil (not disease suppression) using compost tea.
Plant pathogenic bacteria of genus Clavibacter were found in glacial ice across the World. (see Christner, B. C., Mosley-Thompson, E., Thompson, L. G., Zagorodnov, V., Sandman, K., & Reeve, J. N. (2000). Recovery and identification of viable bacteria immured in glacial ice. Icarus, 144(2), 479-485. and other publications). We have found it in underground waters used for irrigation. Is there any projects devoted to plant pathogens underground?
We have a cucumber wilt that is spreading in greenhouses.
I have done some search online and found similar cases in the area (I posted the photos that is similar to the case we have).
Please share your opinion.
I have isolated recently pathogenic for tomato plant bacteria of Chryseobacterium sp. There is a few references about such bacteria as plant pathogens, including one old strain NCPPB No. 583 of Chryseobacterium sp. received as Xanthomonas stizolobiicola from India and isolated from Stizolobium deeringianum (legumes).
Is there any other records of plant pathogenic Chryseobacterium sp.?
It is known that L glucose cannot be used by living organisms as a source of energy because it cannot be phosphorylated by hexokinase, the first enzyme in the glycolysis pathway. One of the known exceptions is in Burkholderia caryophylli, a plant pathogenic bacterium, which contains the enzyme D-threo-aldose 1-dehydrogenase which is capable of oxidizing L-glucose.
So, suppose that we inject L glucose in the stem of a certain plant, What is its fate?
As you know GWAS have become very popular for finding causal variants using natural populations, then why there is a huge discrepancy between the number of GWAS done on hosts (wheat, maize, rice etc) and pathogens (fungi, bacteria)?
There are numerous GWAS paper for different traits in agricultural crops, but very few for identifying pathogen virulence genes or loci.
What are the possible reasons?
The inhibition of spore germination and growth of Botrytis cinerea upon treatment with toxic molecule occured on solid culture medium (petri dishes) as predicted before. Strikingly, the spores germinated normally in liquid medium that contain also the toxic molecule at the same concentration (flasks).
How can we explain such observation??
(See attached pic.)
Dear all. It is known, that climate change and also extreme weather can cause outbreak of some pests and their enemies also. Other pests may be diminshed. The question is: How extreme weather events like heat, drought, storm, heavy down poors, hail, flooding will influence the special diseases of meadow fescue and the special plant protection measures (chemical and biological) in meadow fescue. We found nothing in literature but need more information for risk assessment. Under current climate conditions plant diseases and pests, (mammalians, weeds, insect pests, nematodes and plant pathogens like fungi, viruses, bacteria) can cause considerably yield losses and damages of crops. Climate change will likely lead to increase the frequency, intensity, spatial extent, duration and timing of weather and climate extremes. Does anyone have any information about the impacts of extreme weather events on diseases and pests of meadow fescue? We reviewed the possible impacts of weather extremes on pests (weeds, insect pests and plant pathogens) of meadow fescue by analyzing scientific literature published since 1945, concerning the knowledge about the influences of extreme weather on incidence of these pests.
How can we determine which pathogen (bacteria, fungi, virus) is responsible for a given plant disease from a phenotypic observation (for example: spots, smudges on leaves) ??
Dear all. It is known, that climate change and also extreme weather can influence the outbreak of some pests and their enemies also. Other pests may be diminshed. The question is: How extreme weather events like heat, drought, storm, heavy down poors, hail, flooding will influence the special diseases of alfalfa and clover and the special plant protection measures (chemical and biological) in clover and alfalfa. We found nothing in literature but need more information for risk assessment. Under current climate conditions plant diseases and pests, (mammalians, weeds, insect pests, nematodes and plant pathogens like fungi, viruses, bacteria) can cause considerably yield losses and damages of crops. Climate change will likely lead to increase the frequency, intensity, spatial extent, duration and timing of weather and climate extremes. Does anyone have any information about the impacts of extreme weather events on diseases and pests of rye grasses? We reviewed the possible impacts of weather extremes on pests (weeds, insect pests and plant pathogens) of hop by analyzing scientific literature published since 1945, concerning the knowledge about the influences of extreme weather on incidence of these pests.
We are using the Voges-Proskauer test to differentiate plant pathogenic Enterobacteria (we are using the Rosco Diatab system). Most protocols recommend incubating the cultures at 35-37°C for 48h to produce a dense suspension for the test. Diatabs also recommends incubating at 35-37°C before Barritt’s Reagents A and B are added. I wonder whether the temperature recommendation is because many Enterobacteria are from clinical samples (and so this is likely their optimum growth temperature), or this temperature is needed for the test itself. In other words: do we need to incubate Pectobacterium/ Erwinia etc. at 35-37°C, or at their optimum growth temperature which is of course much lower?
Is fingerprinting method standard method to identify type of fungal plant pathogen and if it is how can I apply it?
I'm studying changes in frequencies of approximately 40-50 SNP alleles in nine regions across a country within a gene of interest in a plant pathogen. to gather this data a large number of samples have been taken from each of the nine sites for six sequential years, and illumina sequenced in a pool. so I will have nine .bam and .vcf files with around a hundred reads (high coverage) each, giving me a number analogous to allele frequency for each snp, for each site, in each year.
I am trying to determine an appropriate statistical analysis for detecting significant changes in allele frequency across this six year time frame. so far I think a CMH-test (probably using the r package popoolation2) will be my best option, with year and allele frequency as the nominal variables, and sites as replicates. however I'm conscious of heterogeneity between sites, which given my previous results is unlikely, but possible.
Would anyone be able to give me any other options? I was also thinking of a GLM with bonferroni correction, but cannot find a convenient way of implementing this with a six leveled qualitative independent variable.
What are the potential effects of on interactions between nonvectors and vectors on the spread of plant pathogens in managed and natural ecosystems?
Previous studies have shown that different herbivore and plant pathogen species elicit different hormone responses in plants.
Mediated by plant defenses, could herbivores and plant pathogens interact to affect the spread of the disease across landscapes?
I want to enhance the basal resistance of tomato to pathogen (fungi). So I was hoping i could get a methodology for elucidating the molecular mechanism of epidermal specification and maintanace as it relates to basal resistance to fungal plant pathogen
I was going to order short peptides 20-30AA conjugated to KLH to immunize a rabbit for antibodies against a plant pathogen.
Fortunately i stumbled across this article which reports KLH-antibodies cross reacting with plant proteins. https://www.ncbi.nlm.nih.gov/pubmed/18992832
It also claims " Polyclonal antisera from herbivorous animals, such as rabbits and chicken, often display non-specific background
reaction towards plant antigens, while rat antisera usually exhibit minimal such cross-reactivity. "
but in our experience with rabbits this has never happened, do you believe rats are actually a better option?
Back to main issue, i do not want to perform affinity chromatography to purify my peptide specific antibodies.Would cross absorption with KLH do the trick?
Alternatively, could you recommend another carrier protein that is as good as KLH in inducing an immune response without the cross reactivity with plant antigens?
Many thanks :)
We have a task for water samples analysis and need to concentrate /isolate all groups of plant pathogens separately for PCR from the same sample. So far, we apply 3 rounds of filtration. Is there any other options?
hello every one,
I am working on soil born pathogen like Fusarium , i want to coat seeds with nano particles to control disease of chickpea and other economically crop. please guide me it possible or not ? if possible then please guide me to procedure for coating
I am interested to use secondary metabolites of trichoderma to enhance plant growth and to control plant pathogens. So How may I isolate cerinolactone from broth culture and whats the function of it on plant body can anyone explain?
i search for standard method to study antifungal effect of plant extracts on plant pathogenic fungi
Sir, i m working on Myco-synthesis of silver nanoparticles against some plant pathogenic fungi. i want to know about the characterization of them .. in which form(like powder or liquid) and if we want to apply it in field how we should use them ?
I was analyzing several examples of plant pathogen interaction (pathosystems) and I was wondering if cell expansion comes first or second to cell division. Of course, I must considered that everything happens after a PAMP sequence of events and also to possible recognition of pathogen by plant cells.
Does anybody have some clues?
Thanks and Regards,
Dr. Joao Paulo R. Marques
I need information about this phenomenon on breeding crops of any disease. Please any job or publication you could share with me would be very useful.
Thank you so much.
Hello, I want to preserve wheat blast fungus in glycerol for long term storage. However, I do not know the procedure for this particular fungus. What should be the concentration of glycerol? Do I need to make conidial suspension and then store it? or What is the whole procedure? please give me some suggestions.
Trichoderma has reached substantial success as a influential biocontrol agent at worldwide level, it is necessary to understand the mechanisms of action of Trichoderma harzianum against plant pathogenic fungi that could highlight to the target specific secondary metabolites of antagonistic fungi for suppressing plant pathogenic fungi.
For my research work I need "POTATO VIRUS X (PVX)". Please if some one provide me Potato Virus X infected plants.
Thanks in anticipation.
Confrontation assay is usually conducted between a bacteria and plant pathogen but is there any method of checking this assay between plant and bacteria?