- Muhammad Usman Munir asked a question:NewSimplest way to check drug release?
How we can check the drug release from nanoparticles to state either drug release from nanoparticles is prompt or sustained release and which method is simplest and accurate?
I need some comments from experts. ThanksFollowing
- Rahul Umakant Hude added an answer:1What type cellophaneuse membrane(specificatins) should be d to perform drug diffusion study of Insitu ophthalmic gel using Franz diffusion cell?
I am working on Insitu ophthalmic gel containing two drugs with molecular weight about 500 and 350. I need to decide the type of cellophane membrane.
for ophthalmic gel to perform in-vitro diffusion study cellophane membrane having pore size 0.22-0.45 micron should be used; having membrane Molecular weight cut-off (MWCO) 10000-18000.
- David E. Bautista-Erazo added an answer:8What is the best method to separate unentrapped hydrophobic drug from a liposome formulation?
To find the amount of drug entrapped in a liposome, which is the best method! Dialysis or centrifugation or any other? also, please specify how we could be sure to discriminate the amount of unentrapped drug and/or released drug from the liposomes using dialysis bag.
Your explanation is awesome, thanks a lot.Following
- Pradeep Paudel added an answer:2Can one answer me what happen if keeping glacial acetic acid instead pbs in drug release assay?
can one answer me what happen if keeping glacial acetic acid instead pbs in drug release assay?
As said by Rafik Karaman sir, it depends upon the nature of the drug you analyze i.e. either acidic or basic. All it matters is pH in my opinion.Following
- Miriam Angeli - Greaves added an answer:8Is that correct to compare the dissolution test of a drug with drug release in the gut ?
As a part of my research I am planning to do a dissolution test to see the active drug release from folic acid tablet and to see the effect of storage on active drug release. i would like to know by giving the controlled conditions (temperature and pH as in the gut) in-vitro, to dissolution test can i discuss the drug release in the gut? is this correct way to discuss the drug release in the body?
In vitro testing tells us how a tablet will behave in a liquid that simulates those in the digestive tract, from stomach to ileum. You can do the tests at the appropriate temperatures too. It will not behave EXACTLY as the in vivo situation, as in the live animal there are so very many other factors, but it will give you an idea.Following
- Sonal Omer added an answer:4How to study drug release profile of an hydrophobic drug?
I have to perform a drug release study of an hydrophobic drug whose solubility in water is 5 mg per liter.Can anyone please suggest me setup of the drug release system ?
thank you sir for such a useful paperFollowing
- Marco Consumi added an answer:7WHich is the most appropriate simulated intestinal fluid for poorly water soluble drug release experiment??
I have performed release experiment of poorly water-soluble drug from different polymer matrix. I have used simulated intestinal fluid SIF conform to USP (pH 7.4 buffer solution). As suspected the release has been close to zero. On the other side, the in-vivo experiment demonstrate the intestinal absorption of the drug. Do exist simulated intestinal fluid appropriate for poorly water-soluble drugs?
Thanks for your answers. I guess if I the change the media (SIF) I lost the "instestinal absorption simulation". THe apparatous 4 is really ineteesting, do you know the most appropriate flow rate to simulate and intestinal release ?Following
- Shimul Halder added an answer:11How can I calculate the % of drug released during dissolution studies?
Can anybody provide me the equation by which i can calculate the percentage of drug release during dissolution of a solid oral dosage form?
can anyone give me the equation to determine initial rate of dissolution??Following
- Raja Rajeswari Kamisetti added an answer:3What is definition of drug release in polymer engineering?
How can I define drug release?
i can tell that drug release occur depending on the the type of the drug, dosage form, type of the polymer used. So the drug release can be defined to occur either by diffusion ( fickian, non-fickian, controlled, anamalous fickian diffusuion), erosion, dssolution. which depend on various factors like pH, temperature, enzymes, electrical conductivty, ....
Polymes again play a role depending on their biodegradability, or not.Following
- Jagadevappa S Patil added an answer:4Should I ignore an initial burst for drug release while examining the appropriate drug release model?
Just between zero, first order, Huguchi and Peppas models.Dear Prabhanjan,
It depends on situation and your requirements. Some time initial burst release may be considered important.Following
- Carlos Alberto Bernal Rodríguez asked a question:OpenWhich plot do I need to make to obtain the determination coefficient (r2), and which is the procedure to apply the mathematical Peppas-Sahlin’s model?
Determination of the release mechanism from a hydrophilic matrixFollowing
- Balavigneswaran Karthikeyan added an answer:6How one should conduct in vitro drug release study when the drug is not soluble in Phosphate buffer?
I would like to study the docetaxel release from Nanofibers/3D Scaffolds where docetaxel is not soluble in water so which is the preferable medium to conduct in vitro drug release study at pH 7.4. Can someone help me out?
u meant to say adding tween or anyother surfactant where the drug soluble? I have not tried any such thing. will that work? wont it cause any problem in the release kinetics profile?Following
- Anwar A Hussain added an answer:21Is it possible for cumulative drug release to exceed 100%?
Paracetamol suppositories made with PEG 4000 and PEG 6000 as suppository bases undergo dissolution test. After 45 minutes, both batches have mean % of drug release that is higher than 100%.
PEG 4000- 150% and PEG 6000- 120%
Why does the cumulative drug release exceed 100%? Is there error during measuring of absorbance or is it some form of degradation or hydrolysis of the medicine during manufacture/storage?
Dear friends, Peroxides exist as impurities in PEGS. If you are engaged in formulation and your compound is sensitive to oxidation be aware of it. You can google the information.(THIS STATEMENT IS FOR INFORMATION ONLY)Following
- Tarek Mosaid Bedair added an answer:5Can anyone suggest the best way for this study as i can't see the sirolimus peak after HPLC at 278 nm?
I coated the metal surface with a mixture of PLLA and sirolimus and i want to study the drug release in human plasma. The loading amount was 125 ug per sample. For the drug release, i immersed the sample in 1 ml of plasma solution and after predetermined time 1d, 3d, 5d, ....... i can't measure the amount of the released sirolimus. could you please inform me how to extract the sirolimus from the plasma solution or any other way?
Thank you in advance
Thank you all of your advice
After searching, i found that the sirolimus is not stable in human plasma solution as the half-life time of sirolimus is only 6 hrs.Following
- Delly Ramadon added an answer:13How do I calculate cumulative drug release?
I'm doing drug release using Franz diffusion cell. The medium is PBS. I'm taking 500uL samples at predetermined times and replace the amount taken with fresh PBS to maintain the sink condition. I'm not sure how to do a cumulative release plot (amount permeated vs time) because the absorbance data that I've got is going up and down( is that normal?).
The data should be calculated using the real amount in the receptor compartment. You should follow an equation in the article above.Following
- Bikash Debnath added an answer:2What are the problems for gentamicin derivatization?
I'm working on controlled release of gentamicin from microspheres. To measure the drug amount I use derivatization method which includes ortho-phthalaldehyde, beta-mercaptoethanol, methanol in 0.04M sodium tetraborate. But after derivatization, I couldn't obtain any measurement at 333 nm neither peak. What is the problem that I did wrong?
Gentamicin is the cheapest and the first line aminoglycoside antibiotic. Intake the Gentamicin for serious gram negative bacillary infection it has gives relatively more nephrotoxic effect in human.Following
- Nilani Packianathan added an answer:3How do I detect hydrophobic drug such as curcumin in PBS?
How can I detect curcumin in PBS? I've run through my samples through HPLC twice but I couldn't detect any curcumin when the absorbance could be detected when I run my samples through UV. I'm doing a drug release study for this.
Curcumin can be separated from curcumin mixture (a mixture of curcumin, demethoxycurcumin and bisdemethoxycurcumin) by column chromatography by adsorbing the mixture on silica gel using mixtures of solvents like dichloromethane/acetic acid or methanol/chloroform to yield three different fractions. The curcumin fraction is further purified on silica gel using chloroform/dichloromethane and ethanol/methanol mixtures as eluents . Methods for the detection and estimation of curcumin have mostly employed the high performance liquid chromatography (HPLC) technique. In general reverse phase C18 columns are used as stationary phase and different gradients of solvents containing acetonitrile/water or chloroform/methanol have been employed as the mobile phase [21–24]. For detection of curcumin, absorption detectors in the wavelength range from 350 to 450 nm range or in the UV region using a common detection wavelength in the range of 250 to 270 nm is simple and extremely useful.
HPLC-diode array and fluorescence detection methods have also been used by several researchers. Liquid chromatography-coupled mass spectrometry has been another versatile tool for detecting curcumin. Of all these the most sensitive methods for detection of curcumin (up to 1 ng/mL) is by fluorescence, by exciting in the 400 to 450 nm region. High-performance-thin layer chromatography methods using aluminium plates precoated with silica gel as stationary phase and chloroform-methanol as solvent have been very useful for both detection and separation.
Pls refer to research article stated below for further information:
The Chemistry of Curcumin: From Extraction to Therapeutic Agent
Kavirayani Indira Priyadarsini
Molecules 2014, 19, 20091-20112; doi:10.3390/molecules191220091
- Jaywant Pawar added an answer:3How can I develop the dissolution medium of poorly water soluble drugs apart from official media given in USP/BP?
Whether it is necessary to compare my developed formulation with the innovator drug release profile in respective dissolution media?
How to develop a dissolution media for existing API? I want to study How is the technology or polymer composition helping us to improving drug release pattern?
Thank you Vladimir and Mubashar Rehman Sir for the detailed information.Following
- Mubashar Rehman added an answer:4Which technique do you suggest to study release of Sorafenib from sorafenib loaded lipidic nanocapsules?
Hi there. I have encapsulated Sorafenib in lipidic nanocapsules with size of 50 nm. I have planned to study drug release by Sink Conditions through using dialysis membrane (MWCO: 100kD) in "PBS-1% Tween 80" as medium. The problem is that approximately all the released sorafenib attaches on the membrane and does not enter to the medium. What do you suggest to solve this problem? Can I add something like blank lipidic nanocapsules into the medium to increase the mobility of the drug? Thanks a lot.
Can you use any other method than using dialysis tube? If your dissolution study is flexible, you may take very low amount of lipid nanoparticles in eppednorf tubes and add dissolution medium. Place these tube in your test conditions. At different time points, take out one tube. Separate lipid nanoparticles and measure amount of Sorafenib released till this time. This method is sometimes used to determine solubility of proteins.Following
- Manuel Roman Pina-Monarrez added an answer:7Can any body help me to learn how to fit drug release data into Weibull Function in Excel?
Hi all. I need to fit my drug release data into Weibull function to know about the mechanism of drug release.
The formula for weibull function is F = 1 − exp(−atb). where F is the drug fraction released at time t, and a and b are constants. b, as a shape parameter, is characterized as exponential (b = 1), sigmoidal (b >1), or parabolic (b < 1).
Can some one help me HOW to fit or put my drug release data into this formula using microsoft excel sheet?
Nauman, I am familiar with Murat advised, Only, in the estimation of ln(-ln(1-F(t))), since F(t) is estimated by the median rank given by the Benard's approximation given by F(t)=(i-0.3)/(n+0.4), then the sample size n has big impact in the estimated b and a parameters. Thus, in order to select the right n, you has to determine first the desired or expected reliability percentile R(t), and based on R(t), determine n as n=-1/ln[R(t)]. (see eq.(35) of the reference paper given below).
In particular, since b is completely determined by the standard deviation of the lifetime data [eq.(41)], and a is completely determined by the mean of the lifetime data [eq.(46)], then, selecting the right n is determinant to accurately estimate the b and a parameters.
My paper where these issues are addressed and solved is "Weibull and lognormal Taguchi analysis using multiple linear regression". which is uploaded to RG. Please observe that in addition to estimate n correctly, the paper also gives the method to determine b and a for a desired combination of the factors that determine your life times. Thus, if you have the factors that determine the lifetimes you can use them in the estimation of the related Weibull family. Any question related to the paper content or how it could be implemented by using your data, please let me know.
- Sonal Mazumder added an answer:9What concentration of nanoparticles should be used for release studies - Is sink condition the determining factor?
How important it is to maintain sink conditions during drug release studies from nanoparticles? What should be the concentration of pure drug and drug in nanoparticles in the buffer to start with? Is there a way to decide that? I have drug incorporated in polymeric nanoparticles.
- Adeel Masood Butt added an answer:2Why is important to lyophilize drug-loaded micelles before drug release curves?
I have drug loaded polymeric micelles, and i read in some papers that lyophilized and other non-lyophilized before the drug reléase.
Usually we lyophilize the samples for stability and storage. As you can not keep the micelles in solutions for long time (for storage), so it is better to freeze-dry. Then you can use the freshly prepared micelle solutions for release experiments or for any other required assay.
Hope this helpsFollowing
- Poulami Majumder added an answer:8How do I assure a sustained release for a weakly basic hydrophobic drug?
I am trying to make a sustained release system of a hydrophobic drug (significantly soluble in DMSO only, not in methanol/acetonitrile/choloroform, etc). The drug is NOT aromatic but has a nitrogen and pka ~ 8.3. It always leads to a burst release when I tried to encapsulate within neutral liposomes composed of DOPC/DOPE/Cholesterol, etc. I dissolved the drug in DMSO, mixed app. amount (10:1 lipid: drug in w:w) with other co-lipids, swelled overnight with water and removed unentrapped drug by centrifugation. Encapsulation efficiency was ~50-60%. But it was released entirely within 3 h. Then I tried to use the citrate salt of the drug (water solubility ~ 1 mg/ml) for remote loading into the neutral liposomes (DOPE/DOPC in 1:1) after forming transmembrane pH gradient. Now, I found no encapulsation at all. Please suggest something so that I am able to reduce the burst release for the drug. Any help is appreciated. Thanks!
Many thanks for your extensive reply. My drug is a nitrogen base containing pyrimidine and piperidine rings with a mol wt of 313 for the free base form. The drug is really leaky because my first trial was to load it into a hydrogel for making a sustained release system and that too did not work for the drug, so I decided to load into the liposomes first and then put the liposomal drug into the hydrogel.
I tried the following:
1. to load the free base form in the lipid bilayer. It shows ~ 80% encapsulation.
2. Loaded an inclusion complex of the drug with beta-cyclodextrin into liposomal aq core with ee of ~ 60%
3. Loaded the water soluble citrate salt of the drug (solubility 1 mg/ml) into the aq core and had ee of ~ 50%.
But none of these does not lead to any improvement of the burst release profile when I put the liposomal drug into the hydrogel. So, I decided to check with transmembrane gradient.
Many thanks for your suggestions. Hopefully this time it would turn out well.Following
- Lefnaoui Sonia added an answer:13How can I increase solubility of my hydrophobic drug (Log P = 5.09) for in vitro drug release and permeation study?
I've prepared nanoemulsion and need to measure the release and permeation of that formulation using franz diffuiosn cell. I've found some of the researchers added SDS (sodium dodecyl sulphate, 2%) or ethanol in PBS 7.4? Which one is the best? Is there any guideline that I can follow for this experiment? My drug is very hydrophobic (0.039 ng/ml - solubility in water) and high solubility in methanol and ethanol. Thanks a lot.
L'ethanol est plus approprié pour votre étude.Following
- Çağla Çibuk added an answer:4What is the reason that the release does not continue? (controlled drug release, HPMC matrix, cefazolin)
I am working on drug delivery behaviour of cefazolin by using HPMC as a matrix. In vitro release of cefazolin from HPMC carried out at 37 C in SBF pH 7.4. 55% of the loaded drug was released within 6 hours and after that we did not observed any release. We did not explain this peculiar behaviour.
We think that either cefazolin are trapped within the HPMC matrix or it degrades and we can not observe the change in concentration.
Thanks to all of you for your answers, I will consider your thoughts.Following
- Nguyen Thuy-Duong Thi added an answer:4Ask for recommendation of using Tris-HCl solution for releasing test?
I want to check the releasing rate of Ca from my bio-material sample by using Tris-HCl solution (pH=7.4). However, I can not figure out which concentration of Tris-HCl solution is close to body fluid. Could you give me some recommendations?
Any help would be respected.
I want to detect the release of Ca from bioactive surface in physiological conditions without inorganic components. The release (dissolution) of Ca in SBF or other solution containing inorganic components effects on the release of Ca due to other phenomena: precipitation and ion exchange, Thus, Tris-HCl solution looks like to be suitable...Following
- Ahmed Agiba added an answer:3Can it be claimed that cyclodextrin based polymer shows enhanced drug release due to inclusion complex formation?
I have prepared a polymer using cyclodxtrin as a crosslinker. After loading of a drug into cyclodextrin based polymer matrix, the drug showed enhanced drug release as compare to polymer without cyclodextrin. There are reports which describes that my model drug forms inclusion complex with cyclodextrin.
inclusion complexation of a drug with B-cyclodextrin loaded into a hydrophilic mucoadhesice polymer is considered one of the methods to ehance solubility, bioavailability and stability of drugs, particularly poorly soluble drugs.Following
- Jeffrey J Weimer added an answer:3How can I determine the n exponent of korsmeyer peppas?
If I have an equation of korsmeyer chart ( log cumulative %drug release and log time) is y = 5.1 x + 3.2 and R2 = 0.8038
how can determine the n exponent to determine the mechanism of release ?
"You can take two points of time with their respective Mt to complete the equation and isolate the k in each."
Two points may give the slope and intercept of a line or solve two equations for two unknowns. It is however a sloppy approach to take in this case. The suggested method should be used only to obtain a first guess. The rigorous approach for a publication-quality report is to use non-linear regression fitting methods to get the parameters and their uncertainties.
We have inexpensive (free), intuitive, user-friendly desktop computational applications today that can perform rigorous data analysis and return fitting values with their confidence limits in the blink of an eye. Why do folks still insist on doing data analysis as though all they have with them is an abacas or hard-copy graph paper?Following
- Anu Rosina added an answer:27Can we crush a sustained release tablet?Is it possible for a person to crush a sustained release tablet for fast action?
crushing a sustained release tablet affects the drug release in which the drug is designed to release slowly in a specific period of time & results in rapid release of the drug and also affects site specific delivery of certain drugsFollowing