Science topic

Wounds - Science topic

Wounds are a wound is a type of injury in which skin is torn, cut, or punctured (an open wound), or where blunt force trauma causes a contusion (a closed wound). In pathology, it specifically refers to a sharp injury which damages the dermis of the skin.
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I have been studying using PRP for chronic ulcer treatment but I was wondering if it could be used for chronic wound healing. I have several patients in the institution I work at that present chronic wounds that due to their advanced age do not heal. As PRP could promote re-epithelization, ¿could you help me in finding some articles that support the use of PRP in wound healing?
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I think you mean PRP for platelet rich plasma is this correct?
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I have herbal extracts obtained by maceration in 80% ethanol that are desired to be evaluated for their wound healing activity using excision and incision wound model on albino rats.
Although the extracts are prepared using the same solvent they have different solubility profile in water (some form clear solution while others results in a homogeneous suspension).
We intend to formulate the extracts into a semisolid dermal preparation using either aqueous cream base or simple ointment base as they are the most frequently used bases with the least intrinsic efficacy.
Is there a suitable method to measure and compare the release of the extracts from the aqueous cream base and the simple ointment base in order to evaluate their suitability.
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Dear Jun,
yes, there are methods.Just read the attached article.
regards
Horst Liebl
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I understand there will be some persister cells in the MRSA biofilm that are in dormant phase. I wound like to know if this kind of cells also exist in planktonic MRSA?
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Yes, persisters can be both seen in biofilm as well as planktonic cells and are resistant to the action of antibiotics
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I have the dimensions of an electromagnet comprising a 0.8mm diameter copper wire, which is used to make a coils around 11mm X 13 mm rectangular cross section core material. The dimensions of the coil after wounding around the core is 25mm X 23mm cross section and 17.6 mm height.
So, how to find the effective number of turns in this for calculating M.M.F and also what other properties can be calcuated from this?
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I think what is required is "what is the number of turns right next to the core, that is equivalent to the actual coil that has a large cross-sectional area?"
http://info.ee.surrey.ac.uk/Workshop/advice/coils/index.html#bib has a list of references, and some equations, and you may be able to relate the inductance of the actual coil to the inductance of a single layer solenoid, and get the equivalent number of turns.
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I understand that both sloughy wounds and necrotic wounds are composed of dead cells, but why necrotic tissue is black while sloughy tissue is yellow?
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I believe that you are describing the difference between "eschar" and "slough". Both of which are considered necrotic tissue. Eschar is a black, thick, dry tissue (think of this as the mummified skin and tissue before it is removed). As eschar begins to autolytically debride, it will change color and texture as it 'liquifies'. The necrotic tissue underneath is typically referred to as "slough". However, "slough" is often the over-generalized word used for "any yellow tissue" in the wound. There are works underway today to help us better define "slough". Check out The Slough Project at the IWII ( https://tinyurl.com/3hr29wnz) and the article "Redefining Slough: A new classification system to improve wound bed assessment and management" by McGuire and Nasser.
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Currently looking at research related to early detection of wounds (e.g. pressure injuries) and projects managing risks (e.g. infection) in wounds using thermography.
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Sorry no, I wish you luck
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I want to study the wound healing on an animal model using nanocomposite hydrogel. For a wound of 10 mm diameter, how much moisture is created that causes the wound dressing to swell and release the therapeutic agent? Usually in literature moisture content around 60-80% is reported for wound dressing.
I would be grateful for your help.
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That depends upon so many factors! In addition to the dressing type, things like the location of the wound, the wound etiology, the stage of healing, the activity level of the patient, and the biobirden all influence exudate levels.
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Hi there!
In his 2014 masterpiece The Body Keeps the Score, van der Kolk claims that traumatized people may "try to cultivate an illusory sense of control in highly dangerous situations" in an attempt to master the physiological and psychological consequences of their trauma. Is there any research which shows that emergency workers (police officers in particular) have a higher incidence of trauma prior to joining the job? That is, is there any research which proves that trauma may be a motivator that pushes people to become emergency workers? I'm acquainted with the idea of the "wounded healer", but I'm interested in the scientific literature on the topic as it relates not to therapists but to emergency workers.
Thanks a lot.
Best,
Marc
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The Maunder et al one is the one I am aware of.
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I'm working on development of drug loaded lipid based topical carbopol based hydrogel for future treatment on diabetic wounds. It includes the characterization study regarding the former things and looking for a journal within impact 1-2. Kindly help me suggesting suitable journals.
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Below are some suggestions within the scope and JCR you need:
Carbohydrate Research (JCR: 2.1)
Journal of Food Processing and Preservation (JCR: 2.2)
Protein & Peptide Letters (JCR: 1.9)
Current Nanoscience (JCR: 1.8)
Medicinal Chemistry Research (JCR: 1.9)
Applied Biological Chemistry (JCR: 1.8)
International Journal of Peptide Research and Therapeutics (JCR: 1.9)
However, I suggest you access the following pages below. They are specific tools of the best publishers, which enable authors to find appropriate Journals for their submission. It's easy to use, briefly, you add the title and abstract of the paper and some Journals that publish works within the scope of your work will be suggested.
I hope this helped you.
Journals Elsevier Finder
Wiley Online
Taylor & Francis
Springer
Regards,
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Dear all,
I know that Uropathogenic Escherichia coli (UPEC) frequently has pathogenicity islands (PAIs), that contribute to their virulence. But I wounding if Avian pathogenic E.coli has the same islands or it is associated only with UPEC
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I think yes, there are many studies that reported the occurrence of pathogenicity islands in APEC. Please check this study as an example:
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Oxylipins play a huge number of roles in animals and plants – including cell-signalling, inflammation and wound response – but did they help ancient microbes ‘invent’ multicellularity? https://buff.ly/3wibP9I
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From Royal Society of Biology RSB discussions
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I'm looking for a software to make panels for histopathology images and wound contraction images. Kindly suggest me a software which can import normal jpg/tiff images and make perfect sized images.
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Shreshtha Gaur….My colleagues use Adobe Photoshop for general purpose work. Those that do specialized image analysis use special software that came bundled with hardware or a variety of open source packages. The most well known and developed is "Image J" or one of the many custom and free variations (download here…https://imagej.nih.gov/ij/) - Image J also has an extensive on-line community that develops tutorials and plug-ins for specific fields of research and reading special file formats. You can read more here…
Another site that offers an overview of some other open source packages is here…
Until you receive other ideas I'd recommend two things; (1) starting with Image J and (2) ask the people at your university what they use. Having a nearby source of help is worth a lot.
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found in post CABG wound and very difficult to clear
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I have also seen a study that found the razors used to shave patients prior to surgery were contaminated
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Any guidelines for different types of wounds, tips for evidence-based practice for wound care will be greatly appreciated.
Thank you
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Yes i shall recommend following articles:
1.Wound healing and treating wounds: Chronic wound care and management
2.Systematic reviews of wound care management:(3) antimicrobial agents for chronic wounds;(4) diabetic foot ulceration.
3.Evidence‐based medicine: vacuum‐assisted closure in wound care management
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I have a set of experiments trying to stop the activity or expansion of the rot produced with these bacteria
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I think that the types of microorganisms to be of great importance in predicting wound healing and infection. Although appropriate systemic antibiotics are essential for the treatment of deteriorating, in addition to controlling the growing conditions
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I'm DNP student that wants to discuss wound care discharge planning for my research. This is a very broad topic, and needing help to narrow the topic down.
Any ideas on wounds that need more discharge coverage?
There are many factors/barriers to discharging patients with wounds, but what is one area you think needs more focus?
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Systematically review the literature using specific keywords might be assisted narrow down specific areas. for example, patient-related factors/family factors...etc
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Endogenous (self - burst) wound healing in patients with impaired metabolism, seeks for attention on many factors like tissue perfusion, peripheral neuropathy, tissue oxygenation, etc. whereas in induced diabetic models and excision wounds vascular and nerve supply are not compromised as same as in humans.
It is seen that other local and systemic factors like, hemoglobin, nutrition, limb activity, digestion etc. are not being focused in experimental studies.
In a nutshell, traumatic and atraumatic wounds, excision/incision and self burst wounds follows different healing methods.
The medicaments showing positive outcomes in excision models of diabetes induced Wistars should not be blindly followed without focusing on other healing factors.
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I agree 100% with Dr. Laborde especially with the first of 14 responses.
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Dear Researchers,
I need your opinion on, in order I want to do wound patch from plant extract, should the extract must be in powder or paste form? I had already dried the leaves, grind using grinder and macerate in methanol. Any idea>
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The plant extract should be in paste form and you can read the paper titled In vitro and In vivo wound healing studies of methanolic fraction of Centella asiatica extract for further knowledge and idea
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  • When human perception of society is so largely primarily visual, how could this integral sense be traditionally excluded by a discipline?
  • Can documentation of more everyday patterns of human behaviour create strong socially reflective imagery?
  • In circumstances that would be overwhelming in a lot of ways, i.e. visually, emotionally, psychologically, how do we know where to start?
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... "how do we know where to start?" feel it!
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hi everybody.
I'm using 75-85% deacetylated medium molecular weight chitosan.
and what if I want to load some wound healer medicine on it.
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Your question can be answered best by Prof Pierre Basmaji, a good friend of mine in Brazil
Kind regards
Horst Liebl
hl@el-cit. com
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I am planning to perform a scratch wound healing assay with endothelial cells. So far, I don´t have experience with this technique. However, I would need to use a 96 well/plate to reduce the amount of inhibitors to use. What are the problems in case of manual scratch with pipette tip 100 µl of a 96 w/p? Does someone of you made experience in scratching 96 w/p? Would the use of a ruler or a line drawn on the back of the plate help me for making straight uniform scratches? thanks:)
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Yes, I have done scratch wound healing on a 96-well plate. I used a 10 ul tip instead of a 100 ul one. also, neither I used a ruler nor lines drawn at the back. however, I used to keep 5-6 wells for each sample. then I used to take images. from each well I was able to take at least 5-6 images. though the scratches were non-uniform, in most of the area thickness of the wound was the same, so out of many images taken, I considered images with the same thickness for the analysis.
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Amikacin is a polar drug meant for parenteral use against gm negative bacteria.In some cases,different forms of amikacin meant for local application are used but can injection amikacin is effective if applied directly on wounds?
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I think that use of antibiotics locali on wound is not good and has no effect on bacterial burden. The local use may also lead to bacterial resistance or even to alergy on this antibiotic.
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What effect would they have if applied on a wound? Antibodies, from another organism, applied on a wound, by the means of ointments and the like.
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Look at this article Victor Anghelescu
Overcoming the challenges of topical antibody administration for improving healing outcomes: a review of recent laboratory and clinical approaches”
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Does anyone have experience doing scratch assays in stem cells in 96 well plate using the wound maker? I notice that after doing the scratch and washing twice, the cells round up and there are gaps between cells although were confluent at baseline?
Wells are coated with matrigel.
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Dear Nihad Abouelazm,
I do not have experience Stem cells but do a lot for primary skin cells especially big challenge for epithelial skin cell. I guess you need to understand your cell behaviour and suitable confluence prior to the scratch procedure. I rather use 48 well-plate compared to 96-well plate as the space too small and could provide high variation of scratching for each well. You also need to control the medium or buffer temperature as it could influence the native behaviour of your cells. Best of luck!
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when we apply the PRP on the wound the GFs will be released, why?
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PRP can be activated in various ways, for example with calcium chloride, autologous thrombin, a combination of the 2 mentioned and also with certain collagens (specifically collagen type I).
In wounds, platelets are activated spontaneously after exposure to the native collagen present in the connective tissues. However, collagen is a weak activator. Adding one or more of the others (CaCl2/ Thrombin/ combination) stimulates a more rapid and/ or sustained release of GFs. As to your final question, PRP can certainly be activated to release GFs in a test tube provided that one or more of the "natural" activating factors present in all wounds (such as collagen, calcium etc) is present.
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I am interested in pursuing my masters thesis research on the use of Tilapia skin grafts in wounds of dogs. Any inputs would be appreciated.
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I following the best answer.
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How to evaluate extraction socket healing (when the wound is primarily sutured)? Most of the studies use Landry and Turnbull Healing Index. There is also the H2O2 bubbling test. Do you have any other suggestions regarding this?
Thanks!
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Hi,
You could take a look at healing index such as Vancouver, Manchester or mucosa sac index.
Alternatively, you may also evaluate wound closure macroscopically.
Good luck
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What is the role of local Carboxy therapy in chronic wound treatment?
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Please take a look at this useful RG link.
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What is the role of Ozone therapy in chronic wound treatment?
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Please go through the following RG link.
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Hi, In our study we are going to evaluate the effect of 3 different treatments on burn wound using rat models. what should the P-value cutoff be For statistical analysis of our q-pcr output and collagen volume fraction results from image j?(0.01 or 0.05)
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Hello Sepideh,
If it were a matter of one size truly fitting all cases, then you obviously wouldn't have to ask this question!
The answer to the appropriate level of risk for type I ("alpha level") as well as the acceptable level of risk for type II ("beta level") errors in hypothesis testing really should depend on the consequences of being wrong.
One consequence that large scale replication projects have shown is that a lot of published studies that showed "statistical significance" in the original study fail to do so when a replication is attempted. That's part of the reasoning underlying Cristian Ramos-Vera 's suggestion of a more conservative alpha level. Is it possible to quantify the costs associated with a Type I and Type II error in the framework of your proposed study? That might help you to set realistic levels.
Good luck with your work.
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It is unlikely that I will get a specific answer to this question, so I propose to start a discussion on the geometry of the standard model.
The standard approach to this question is that, in addition to space-time, the space of internal symmetries of the Lagrangian is postulated, which determines the equation of motion of an elementary particle. In other words, in the standard approach, the invariance of the group action on the equation of motion is used as an additional internal space.
It would be interesting to know what kind of geometry is hidden behind the standard model of elementary particles. In this regard, let me share my thoughts on the geometric foundation of the Standard Model in this discussion.
The author's interpretation is as follows. Matter moves along the surface of a seven-dimensional sphere. At the same time, the vacuum form of matter is a Clifford torus S3xS3 with three-dimensional spheres moving in the process of evolution in circles so that the radius of one sphere increases and the radius of the corresponding circle decreases, and vice versa, the radius of the second sphere decreases, and the radius of the corresponding circle increases. As a result, the shape of the vacuum is the product of the Clifford torus and the time torus S1xS1. In this case, the Minkowski space-time (with the signature +1,-3) is wound on the product of a three-dimensional sphere of a large radius and a circle of a time torus of a small radius, and the additional (dual) Minkowski space (with the signature +3,-1) is wound on the product of a three-dimensional sphere of a small radius and a circle of a time torus of a large radius. Thus, a doublet of Minkowski spaces, which is an 8-dimensional space with a neutral metric is wound on the product of the Clifford torus and the time torus.
Within the framework of this model, elementary particles are associated with the minimum length closed lines of the product of the Clifford torus and the time torus, and the mass of elementary particles is associated with the pseudo-Euclidean length of the corresponding curve in 8-dimensional space with a neutral metric. Thus, massless particles lie on a compactified isotropic cone. And the most interesting thing in this geometric model is that the group U(1)xSU(2)xSU(3) naturally arises as the symmetry group of the compactified isotropic cone of the doublet of Minkowski spaces.
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So, what can we say about the masses of fermions within the framework of the geometric interpretation of their closed curves on the product of the Clifford torus and the time torus? First, it should be noted that closed curves lying on a compactified isotropic cone (generating the gauge symmetry group) have zero length, and therefore can be associated with gauge bosons. Second, since for stable particles the time torus can be replaced by a circle, we will first look for minimal closed curves that entwine the product of the Clifford torus and the circle.
In addition, by studying the topology of nodes on the product of spheres S3 × S3 × S1, we can simplify this product to a 3-dimensional torus S1 × S1 × S1 without prejudice to understanding the topological properties of the node, and for clarity consider a closed ribbon lying on a 2-dimensional classical torus S1 × S1. Then, due to the fact that the node on the torus corresponds to the fundamental group of its complement, isomorphic to the corresponding braid group, it is quite fair to compare the elements of this group with the fundamental components of the elementary particle, as is done in the topological Bilson-Thompson model. Finally, note that developing this approach to the case of unstable particles of subsequent generations of fermions, it will be necessary to turn to the 4-torus.
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I have an ICU patient who is known to have ESBL-producing E. coli colonisation detected on numerous rectal swabs in prior admissions. Now, he has E. coli bacteremia with isolates not ESBL-producing. How is this possible? What could be the likely source of his bacteremia? He has no wounds. Thank you in advance for sharing your knowledge.
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plz share with us the outcome of your patient
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Maybe because the Minkowski space is actually wound on the manifold S2xT2, just as a pseudo-Euclidean plane is wound on a torus by mapping isotropic straight lines to the defining circles of the torus.
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I understand that you do not want to abandon physical models based on the pseudo-orthogonal subgroup of the Lorentz group. Of course, this is your right, but nevertheless, I note that I prefer the physical model, which is based on the dynamics of vector fields in an 8-dimensional space with a neutral metric, obtained as the Finsler product of a doublet of Minkowski spaces with an inverse metric. The gauge symmetries of this model are hidden in the dual Minkowski space, which is wrapped around S1×S3 .
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which the best wound model for type 2 diabetes wound studies?
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You can follow this book "Drug discovery and evaluation" by Vogels.
You will get so many animal models along with modification for in Vivo models.
Best wishes.
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I am looking for a best scaffold for cell delivery to the skin wound. I am wondering about acellular natural scaffold but nowadays synthetic scaffold specially made of polymer e.g PLGA, is really interesting. Could you please let me know your experience in these field?
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Poly lactic-co-glycolic acid (PLGA) has been among the most attractive polymeric candidates used to fabricate devices for drug delivery and tissue engineering applications. PLGA is biocompatible and biodegradable, exhibits a wide range of erosion times, has tunable mechanical properties and most importantly, is a FDA approved polymer. In particular, PLGA has been extensively studied for the development of devices for controlled delivery of small molecule drugs, proteins and other macromolecules in commercial use and in research.
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53 Y Female was exposed to boiled water which fall on her abdomen. She left the burn untreated and scab formation occurred. TBSA expected 1-4% , probably superficial partial thickness burn. How would you manage this burn? Peel the scab and create a moist wound environment? Or what procedures would you follow now after the scab has formed?
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You would need to determine the depth of the burn and predict healing potential: whether it will heal spontaneously or would need excision and grafting. If grossly infected the wound needs to be debrided early. If not the wound could be dressed with silver sulfadiazine for a few more days to see if the "scab" separates off which would allow you to reassess better.
If you still would have a layer of ?eschar after a week the burn wound is most likely deep and would need excision and grafting.
Posting a picture would help a lot.
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Given parameters:
Stroke:+/-25 mm
Excitation: 5.3V rms, 2000 hz
Bobbin: SS tube, 3.5 mm ID, 4.65 mm OD
Primary coil is wound over the entire length of bobbin
Two secondary coils are wound over primary -- one in each half section.
Sum of two secondary outputs: 3.72 V rms
Determine:
Length of bobbin, number of turns of primary and secondary coils
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Construction of Linear Variable Differential Transducer? - MATLAB ...
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I am to study the wound healing of infected wound in animal model. However I am not sure about the animal model to be used. Whether swiss albino mice or Wister albino rats or Sprague Dawley rats would be appropriate for the study that involves an infected wounds on the mice/rats. .
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Thank you Shaymaa Fadhel for the kind information
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I am looking for ways in which I can compare wound healing among different treatment groups
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Shaymaa Fadhel even with several measurements at different time points?
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We have evaluated the wound healing potentials of a compound at a particular concentration in ointment form in laboratory animals. Now, we are preparing different types of topical formulations (gel, ointment, spray etc) for wound healing studies on clinical wounds of domestic/livestock animals. Does it require to test each topical formulation again on laboratory animals before proceeding for study on clinical wounds?. Please, also tell me that what should be the other guidelines which I have to follow before going for study of our topical formulations on clinical wounds of livestock animals (sheep, goat, cattle etc) for the drug development process.
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I think yes every formulation should be tested because excipients will be different in each formulation and may lead to increased or decreased bioavailability.
Regards
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This question is for wounds surgeons and consultants working in area of wound practice and research
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There are several software and 3D cameras that give very good results, but are all rather expensives. I think that a good camera, such as last generation mobiles, with a caliber and a good illumination could be an acceptable solution. For me is very important the quality of the shot: with correct illumination and angolation.
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Hi everyone. I have recently observed that after I made the wound and switch the medium, in some wells the cells look like dead.
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Obviously when you changed medium, the cells were drought. Try to keep cell under moisture while changing medium. Did you scratch after removing the medium? If yes, that would cause the same problem because the cells are exposed to air for too long.
Hope this answer helps.
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Dear all,
I came across your paper 'Fully convolutional networks for diabetic foot ulcer segmentation' because we are working on wound segmentation and I was wondering if you can share the images of foot ulcers and theirs ground truth images, so we would be able to carry out real experiments and compare it with other implementations. We appreciate your collaboration and your work will be cited in our possible outcomes.
Thank you very much,
Karl.
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On your web pages there is an article by Telichowska KS with bibliographic data of the Integrative Molecular Medicine (IMM) journal (Telichowska KS (2019) Wound coverage by linen dressing accelerate ulcer healing. Volume 6: 1-3. Integr Mol Med, 2019 doi: 10.15761/IMM.1000371)). Unfortunately, there is no such article on the IMM's page. The article was withdrawn a few months ago from OAtext publisher pageges, remove it from your pages.
Jan Szopa, professor of biochemistry University of Wroclaw
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Yes you can if you is the only author or all authors do the same procedures: 1-Select the withdrawm manuscript from your puplication list 2- Click in the square icon (placed in the right of share rectangle) 3- Select remove 4- Select Permanently delete the publication page from ResearchGate
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What are some biomarkers that can best describe the wound inflammatory phase during the wound-healing experiment in rats?
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Check this
Inflammation Biomarkers and Correlation to Wound Status After Full-Thickness Skin Grafting
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Good day,
I will really appreciate your brilliant minds on the above question.
I am working on developing a chronic wound in vitro model, specifically diabetic foot ulcer. I am looking for an effective way of rendering the choice cells hyperglycemic, to mimic diabetic conditions. Any useful idea please?
Thanks a lot,
Mirella
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Just add sugar what humans comsume to go into the state of hyperglycemia.
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The wound myiasis causing flies of animals are potential human myiasis agents. Monitoring the animal myiasis helps to more precisely identify the dominant and aggressive species in the province.
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No I dont. Veterinary people are in charge of animal health and do a good job.
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I am working on subject called "Father wound" in psychology. I would like to hear from people who work(ed) on the same subject.
Thanks a lot
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Healing the Father Wound by Kathy Rodriguez may help you with your topic.
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Dear all,
We want to model a pressure vessel using WCM (Wound Composite Modeler). We have built a model  by following  the instructions in user manual and examples. For post processing, UVARM subroutine was generated by WCM Plug-in. Then we have run our input file with UVARM subroutine; however, we have encountered an error as expressed below;
" Abaqus Error: The executable Standard with system error aborted with system error "illegal memory reference". (signal 11). please check the .dat, .msg, and .sta files for error messages if the files exist." 
Since we have no internet connection at our computer where input files are run, we can not generate system information to report. When we search the error on the internet, common offer for solving problem is avoiding parallelization but we have tried it with one cpu and we have encountered with same problem again. 
By the way, to compile and run our model, we made some changes in UVARM subroutine file. It is that logical definitions were changed into integer definitions (e.g. false->0 and true->1) because we use gfortran as compiler.
Could you please inform us about the possible solutions to our problem? We're looking forward to your reply. Thank you in advance.
Best Regards,
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Hi, does this error persist if you use another compiler?
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Phenytoin is known to increase the fibrotic growth of ginigiva when administered in therapeutic doses in human . Is it expected to facilitate fibrosis in wound area during healing
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Sounds like a bad idea. Phenytoin is a small molecule, and would presumably get into the bloodstream or lymph when added to an open wound. As such, it could cause fibrosis at distant sites.
Let the body do it's natural healing, or use a wound-healing treatment that's local.
Also, by what mechanism does phenytoin cause fibrosis?
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The alpha tocopherol is sold as an ointment while the aloe vera is sold as powder. I am concerned with making the final volume too bulky for topical application
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Aloe vera is also commercially available as gel preparations which should be easy enough to mix with tocopherol, a blender would provide good and even mixing. If unavailable, the powder itself can be reconstituted with distilled water, the proportions would usually be given on the package. For example, if using 50:1 micronised freeze-dried aloe powder, the mixing is 99 parts water to 1 part aloe for the preparation of a clear solution like juice. If no instructions are provided perhaps you would have to experiment with the proportions, maybe starting wth 80:20 to get a gel form. Good luck!
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can treat acute burn wound with amniotic membrane graft ?
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You mean that the using of amniotic membrane are as biological covering ok, but I believe that in 2nd and 3rd degrees diagnosed burn
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I'm looking for the typical values for Mass transfer k used in the calculation of concentration in membrane wall (cases of spiral wound and flat membranes)
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thank you for your answers
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I need your precious suggestions,
How may i carry out an experiment, The use of nano-medicine for burned wounds. .
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There are several papers and literature on this topic, please see:
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In ancient Greece, hoplite gained Kleos, glory, by dying in battle. If they were so injured that they could no longer fight, they would not be named on their tomb, therefore gaining no kleos. What kind of reception did they have when they returned to the polis, alive but no more fighter? Did the psyche of the particular polis affect this reception? Comparing the democracy of Athens with the military camp mentality of Sparta.
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In respect of a slightly later period, both Arrian (II.67.1) and Curtius (III.viii.14 - 16) report on an incident where the Persian army massacred and mutilated a number of sick and invalid Macedonian troops who had been left behind at Issus while the army continued to march towards Syria where they thought the Persians were waiting.
There is no suggestion that these sick and invalid Macedonians had been abandoned. Rather, it would appear that they had been kept with the army till then as it marched through Cilicia, where Alexander had himself been seriously ill. (See my chapter The Road to Issus.) They were left behind at Issus presumably under care because Alexander was in rush to face the Persians, while being ignorant of their own change of position.
We know Alexander took a number of physicians with him on the expedition, and despite the unfortunate outcome, the story does point to a culture in Alexander's army at least of looking after the sick and invalid. This was probably influenced, given Alexander's own education and cultural leanings, by the Greek culture, at least in places like Athens, at the time.
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There are tow types of induction generators used in renewable energy generation
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Hello there,
Wound rotor type of induction generators are the most popular choice in the present time to convert wind energy to electrical energy due to its ability to control active and reactive power with the help of power converters.
However, the cheaper squirrel cage type might be suitable for rural autonomous grids.
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Last week expert Austin Ruse President/C-Fam, Editor & Publisher/Friday Fax (https://c-fam.org/) raised the alert that the UN is negotiating a new hard-law treaty that will make Church teachings crimes against humanity.
How could this possibly be?
Here’s how he explained it.
" UN Member States are now negotiating a new hard-law treaty on “crimes against humanity.” These are generally regarded as those crimes that offend all of humanity, such as the genocide of the Jews by the Germans during World War II.
The new treaty very well may include the following:
“Sexual orientation and gender identity”
And
“Forced pregnancy”
Please knew we oppose all unjust discrimination against those with same-sex attraction. However, we cannot support the idea that opposing “sexual orientation and gender identity” as a new “crime against humanity.” This would criminalize the teachings of the Church.
We faced the notion of “forced pregnancy” when the International Criminal Court was negotiated twenty years ago. In the hands of the left, “forced pregnancy” means that a woman cannot get an abortion.
Working with UN Member States twenty years ago, we got it defined as the repeated rape of a woman for the purposes of changing the demographic profile of a country, literally holding a woman prisoner until she gives birth.
But, the left is back again and they want to insert “forced pregnancy” into the new treaty but without a definition so they can define it later against the unborn child.
This is how the new treaty will criminalize the teachings of the Church. And this is why we oppose this new language, and why we need your help."
I think that it is a problem for all humanity, it is not only a problem for catholics like myself. It seeks to force pro-abort legislation in all countries that have some protection for the unborn. If this UN treaty is approved, besides multiplying the number of aborts, many more women would be dangeroulsy wounded in their feminity because the post-abort syndrom, and beside the problems of an aging population would increase without limit. This is really irresponsably encouraging an inhumane behavior. For what use is the UN today?
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Breaking News! Major Transgender, Abortion, and Sexual Rights Controversies Erupt at the United Nations
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Use of Pistacia lentiscus in the treatment of excisional wounds.
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The answer to your question is "Yes". Please have a look at the following RG links and PDF attachment.
Thanks!
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Hi everyone,
Few days ago we released a male bottlenose dolphin entangled in fishing gear remains in Ecuador. Although we have noticed before some deformities in the tail of this animal, it is just until now we could see them in more detail and take pictures. We noticed the tail of this animal was damaged and deformed three years ago when he was hurt apparently by a boat propeller.
Flukes surface and borders were covered with dozens of hard nodules of different size (2-10 cm in diameter) where fishing gear remains was hooked producing blooding wounds. Nodules look firm, well defined, covered with skin and did not appear externally infected. On flukes surface nodules tended to spread. There is no particular pigmentation, although in those nodules in the borders the skin was pealed off. No nodules were noticed in other parts of the dolphin's body. It is not clear how serious is this particular condition but apparently it does not interfered with his normal activities. Up to 2018 this and other male companion were the males with highest rank in this dolphin community.
A video of the dolphin rescue with close up images can be seen in this link:
Thanks in advance
Fernando
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Hi Fernando, these look like granulomas. Lobomycosis-like disease is a possibility, fibropapillomas another one. We have seen the latest in Irrawaddy dolphinsFusariosis is another possibility (please see attachment).
Cutaneous warts/papillomas look different:
Hope this helps. Marie
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The angle found by incremental encode interface is reset to zero by the index signal. In perfect situation, the mounting of the encoder should guarantee that the index happens at the very instant of having the corresponding stator and rotor phases magnetically aligned. Under this condition, the angle read from the encoder truly expresses the angle of the rotor magneitc axis with respect to the stator magnetic axis (e.g. magnetic axis of rotor phase a with respect to magnetic axis of stator phase A, b with B and c with C).
The mounting of the encoder with the rotor shaft doesn't necessarily follow this perfect assumption especially in laboratory setups for DFIG research. Therefore, It can result in random position of the index signal with respect to the alignment position and consequently incorrect readings of the angle enclosed between stator and rotor magnetic axes of the wound rotor induction machine.
The question is:
What is the procedure to be followed to quantify the possible angular offset by encoder and thereby nullify this offset?
Taking into consideration that the following measurements are available: three instantaneous stator voltage, three instantaneous rotor voltages, direct encoder angle reading
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Sorry for documenting the answer in handwriting
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Any tool, publication, or guideline can help to find out the characteristics of wounds?
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This publication will be helpful for your study. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1360405/
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Does anyone have cell culture experience w/ human mammary epithelial cells &/or has tried to induce EMT in any cell line including epithelial cells?
Also any suggestions on doing scratch wound assays with human mammary epithelial cells would be helpful.
Thank you.
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I have the following experimental experiences;
4T1 murine breast cancer cells were treated with recombinant TGF-beta for 12-96 hours and checked by quantitative RT-PCR and WB (E-cadherin, CD44 variant isoform, N-cadherin, vimentin, CD44 standard isoform, ZEB1)
MDA-MB-231 human triple negative breast cancer cell line was exposed to H2O2 and checked by by quantitative RT-PCR, FACS and WB (E-cadherin, CD44 variant isoform, N-cadherin, vimentin, CD44 standard isoform, ZEB1, Twist)
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There are many types of wounds with different classifications,i need the clinical classification with references, please
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Following
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I am trying to stimulate T47D and SKBR3 cells with 10ng/mL EGF for my wound assay. Not many people that I know of in my lab have stimulated cell lines other than BT20s with EGF, but it works at 10ng/mL after 12 hours. I am not getting good stimulation of migration with EGF for T47D and SKBR3 cells. Are there other growth factors that are more commonly used with these cell lines, like heregulin or TGFa?
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I would suggest PDGF and you will get good results.
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Is there a relationship between fatherlessness and work behavior? Do you think father wounds effect the work life? I would like to hear different opinions. Thanks
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Jale, I am fascinated by your question. Can you please explain a little more about the background to this question in order for me to gain a deeper understanding?
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Dear Sir/Madam,
My present work in analysis of composite pressure vessel using abaqus. for that in need of WCM Plug-in, i dont have anyidea regard this because am beginner to this software. please do kindly suggest and help me,
thank you
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thank you @ mukesh kumar hope this will help for me
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Hey Guys,
I usually use ultracentrifugation in the last step in Lentiviral production and I was wondering if I could use the FLP chromatography instead of the regular method ? which offers higher yield and which can be used in relatively large scale with good titet?
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It should work.. Take care of the buffers u use for eluting. Keeping in mind the intactness of the virus. Do let us know.. All the best. I have experienced min 40 % loss in the titre while using ultra centrifugation. However sorted the green positive cells using a sorter and used for analysis.
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In Plant Tissue Culture, after surface sterilization of explants with Bavistin, Tween80, Ethanol, Mercuric cholride or watever....only the surface of the explants are sterile...once cuts are made in the explants, endophytic fungi comes out and contaminates the explant..Can I surface sterilize the explant after causing wound/cut to the explant to achieve sterility or would this prevent callus formation?
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Yes, you can surface sterilize after the explants cut, but it will certainly affect the explants growth. However, you cannot eliminate endophytes in this way. You have to follow different approaches for the endophytes like selection & types of explants, culture medium, sub-culturing, use of antimicrobial solution in the medium etc.
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The attached picture shows the the type 5 of wind energy conversion system. As it can be clearly seen that the generator operates at a fixed-speed and it is directly connected to the grid.
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I think it depends on whether it's easier or cheaper to perform the frequency stabilising process mechanically or electronically. Normally one would say electronically - but in this application of high power and low speed, and where the input is mechanical, then a mechanical converter might prove better.
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In some cultures it is a common practice to treat wounds with lime as a disinfectant. I couldn´t find any scientific evidence for the use of citrus fruits on wounds. Does anybody of you know literature concerning the usefullness or effects of citrus fruits in connection to wound treatment.
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It is a huge mistake to think that wound healing is affected by what you put on it. The factors that are involved are 1) Arterial supply - the reason that many wounds on legs don't heal is because of peripheral arterial disease. 2) swelling - the reason that wounds on the face heal so quickly is that there is no swelling but wounds on legs frequently don't heal because of swelling which increases tissue tension. Infection is not an issue and we have to stop treating all wounds with antibiotics because we then just produce multi-resistance
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In some cultures it is a common practic to disinfect wounds with the use of lemon or lime. Does anything of you have scientific evidence of the usefulness of this approac? I didn’t find any research on wound treatment with any citrus fruits. ple let me know if you have more informations.
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I suggest you to have a look at:
It is not about wounds but it gives ideas about the disinfecting power of citric acid and others. Many refererences too.
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who kindly help me ...What is the mechanism or path way of essentail oil (different concentration ), when use for healing wound of skin rat..
best regards
Thanks
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Maybe the following papers will help you:
1. Han, X., & Parker, T. L. (2017). Biological activity of vetiver (Vetiveria zizanioides) essential oil in human dermal fibroblasts. Cogent Medicine, 4(1), 1298176. https://doi.org/10.1080/2331205X.2017.1298176
2. Hiroko-Miyuki Mori, Hiroshi Kawanami, Hirohisa Kawahata and Motokuni Aoki. Wound healing potential of lavender oil by acceleration of granulation and wound contraction through induction of TGF-β in a rat model.BMC Complementary and Alternative MedicineBMC series – open, inclusive and trusted201616:144
3. Will Wood, Celia Faria, Antonio Jacinto.Distinct mechanisms regulate hemocyte chemotaxis during development and wound healing in Drosophila melanogaster.The Journal of Cell Biology May 2006, 173 (3) 405-416; DOI: 10.1083/jcb.200508161
4. Karsten Gronert, Neha Maheshwari, Nabeela Khan, Iram R. Hassan, Michael Dunn, and Michal Laniado Schwartzman.A Role for the Mouse 12/15-Lipoxygenase Pathway in Promoting Epithelial Wound Healing and Host Defense.J. Biol. Chem. 2005 280: 15267-. doi:10.1074/jbc.M410638200
5. Alexander JW, Supp DM. Role of Arginine and Omega-3 Fatty Acids in Wound Healing and Infection. Advances in Wound Care. 2014;3(11):682-690. doi:10.1089/wound.2013.0469.
Mariana Barreto Serra, Wermerson Assunção Barroso, Neemias Neves da Silva, et al., “From Inflammation to Current and Alternative Therapies Involved in Wound Healing,” International Journal of Inflammation, vol. 2017, Article ID 3406215, 17 pages, 2017. https://doi.org/10.1155/2017/3406215
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Hi Dear All:
I´m searching clinical evidence in relationship about How a human been or a cohort (better) capsize to an impact in coporal dramatic changes focused in skin impacts (Deglobing, Burns, wounds in special areas etc..).
How can we help improving their outcomes (patient outcomes)?
Thanks a lot,
Martha Uruena-Cartagena., MD
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We had for a while a psychlogist in the wound team.
We defined 3 different topics:
- stress
- depression
- compliance that is now rather moved to the word consensus and we moved to look for self recruitment : the fact that the treated person is looking by himself how he can help to recover as well as possible.
I would add the social workers and the behavior and pysio-therapist team.
Obviously all the different components have to be adressed anf the core wound team is also like an orchestra conductor with the real solist being the"patient" at the central stage.
With my best regards
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Hello every body,
I performed a study test in the mouse wounds and I used the punch skin biopsy to create wounds on the mouse skin.
I'd like to know the best way to cover the wounds.
I used the tegaderm but unfortunately the wounds form a dry layer on the top of the wound which inhibit me to take an accurate measurements for the wound diameter and area.
Could please any one help me and give me an advice about the best way to cover the wounds.
Thanks in advance
best
Ayman
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10% potassium permanganate
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I need to design a spiral wound membrane for waste water treatment to bring down the tds from 3000 to below 100. Flow rate is around 2000 lph.
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Hi Bharti,
One approach would be using data available on commercial membranes. You have given a salt range in brackish water RO, with required rejection around 97%. A commercial Dow Filmtec XLE membrane for brackish water RO can give 98.7% rejection with flux of 56-70 L/m2hr at 125 psi (as per the manufacturers). I assume 2000 lph is feed flow rate. Now you need to pick a recovery % and calculate what your permeate flowrate will be. Dividing that number with the commercial flux should give you the area of membrane required for pressure of 125 psi to carry out your filtration.
This is a simple approach for rough estimation only.
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I need to measure the amount of newly formed collagen in primary sutured cutaneous incisions for comparative reasons between different treatments using Hematoxylin and eosin histological sections. Can anyone help me how to perform this using ImageJ software please. I appreciate receiving step by step detailed instructions.
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Hi Davoud,
On ImageJ, first step should be to separate the RGB channels and select the one where the collagen is (blue for trichrome staining or red if you used Sirius red) (Image>Colour>Split channels). Then, you have to make a threshold to select only the collagen signal. This depends substantially on the quality of your images. If they have a lot of noise, try to do a manual B/C adjustment first (Image>Adjust>Brightness and Contrast) and then the threshold (Image>Adjust>Threshold) to select only the collagen as black and the rest as white. Go to Analyse>Analyse particles and get the area for each collagen region. You can finally sum the values and get the amount of collagen on your picture.
Hope this can be helpful! Best regards.
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I have implanted bilateral cannula in the nucleus accumbens of adult rat. After one month of implantation, the rat some how removed the cannula along with dental cement. Since we used this rat to study drug addiction, I think this might happen sooner or later. Can I implant the cannula again to the same brain region to this rat after the wound is recovered? or should I sacrifice this rat?
thank you
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It is generally standard practice (at least in my experience) to sacrifice the animal if their head-mounts come off. Unless you're using dialysis probes/cannulas, generally these should not be coming off. Did the rat have an infection underneath? If so, then more sterile surgical techniques need to be used. Making sure to properly disinfect tools in-between rats and making sure the skull is dry before laying down the dental cement are important factors for maintaining healthy head-mounts in my experience. I also use thinner, more "liquidy" layers of acrylic as the foundation to make sure it is rock solid. Lastly, make sure you're mounding up the acrylic to the very top of the cannula as much as possible to prevent the rat from messing with it and bending the cannulas or pulling them out.