Wounds - Science topic

as a my undergraduates research, i have an idea about develop anti bacterial coating using chitosan and aloe Vera gel. So, after develop coating it should be apply on external surface of surgical tape. The main reason is reduce the bacterial contamination around the wound sometimes because of bacterial infections wound can be increased and difficult to cure. Also i think apply biodegradable adhesive agent to composite bind on external surface of surgical tape. Can i know what are the can be used biodegradable adhesive agent and any suggestions for my research topic....?

Usually in diabetic wound , the level of growth factor (GF) like PDGF are usually redice/decrease. Is it possible , it can be imbalance. Like due to diabetic wound , the level of GF sometimes can be increase

In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended? If no axillary lymph node dissection was not performed before.

Endoform™ Antimicrobial is indicated for the management of acute and chronic wounds including partial- and full-thickness wounds, pressure ulcers, venous ulcers, diabetic ulcers, chronic vascular ulcers, tunneled/undermined wounds, surgical wounds (donor sites, grafts, post-Mohs surgery, post-laser surgery, podiatric and wound dehiscence), traumatic wounds (abrasions, lacerations, second-degree burns and skin tears) and draining wounds.

I am interested in looking at male attachment, including the development of attachment wounds, systemic and cultural factors. I’d like to specifically tie together the potential impact of healing attachment wounds in GROUPS of men. I’d like to better understand the mental health implications on individuals and society with men who do not have secure attachments. I’m also looking into equine work as a substitute for groups.

Any leads or thoughts are welcome!

If there are parallels studies of attachment wounds in female-identified ed populations, I’m interested in comparing approaches, systemic and cultural factors.

Thank you!

Hello Friends

I have been trying to do scratch wound closure assay in semi-adherent cancer cells, whenever I put the scratch, either a whole layer of cells comes off and there are many large aggregates of cells floating, and the scratch is not neat. I am trying to assess migration potential in these semi adherent cells in vitro. Please suggest alternatives or possible modifications to have a solid and clean scratch. Thanks

I have a mouse skin wound healing model, and it's suggested to give pain management after wounding procedure. It's known that carprofen is a nonsteroid anti-inflammatory agent, acts as a multi-target FAAH/COX inhibitor. Can I use carprofen as a pain reliever after mice surgery when inflammation is an potential effector in my study? If not, could you advise on some appropriate medications?

The hydrogels I have prepared are most like thin membranous sheets. I want to carry out full-thickness wound animal (male rat) studies (6 mm diameter). Should I cut the hydrogels also 6 mm or larger than that? Should it be applied on top or inside wound?

I want to assess the cell migration rate through scratch wound assay for the hydrogel. I have read the literature and in some research articles it is mentioned that DMEM media was not supplemented with FBS (10%) as it supports the growth of the cells so it will effect the results. But in other studies only term culture media has been used. Kindly guide me whether FBS should be added or not into the DMEM.

Thanks in advance for your guidance and time.

Post burn wound healing cosmotic appearance its relation direction of debridement of burned tissues and the the effect on body image of patient as well as quality if life so i am in need to understand the relation the debridement with post burn changes in cosmotic appearance of patient

The Quill barbed suture is a proven wound closure device that eliminates the need to tie knots during soft tissue approximation. Quill uses a running stitch to hold wound edges together with the strength of an interrupted suture. The barbs lock the suture in place, maintaining tension the length of the wound.

A prototype of the MsM harvesting device with Metglas 2605SC

laminate bonded on a cantilever beam wound by a pick-up coil.

Explanation of formula "Um = Metglas layer strain energy"

Paper: Magnetostrictive vibration energy harvesting using strain energy

method

Saber Mohammadi*, Aboozar Esfandiari

We our looking for feedback on our wound scratch assay. We are using HTR-8/SVneo cells.

This is our current protocol:

1. Create a wound in each well by scratching the confluent monolayer with a sterile 200 mL pipette tip.

2. Wash cells with PBS once to remove floating cells.

3. Add fresh serum-free media (1640RPMI ATCC mod.)

4. Image cells immediately after wounding, as well as at 6 h, 12h and 24 h after wounding.

Thank you in advance for your help!

Hello,

I have been trying to search for these machines for 2kw or 2.5kw rating but I couldn’t get a suitable vendor or company In USA. Can anyone please share about purchasing machines ( anyone who is working in wind system research lab who have pmsm pmsg dfig installed). Need information regarding coupling. Please, I need some input.

thank you

Shipra

The Scriptures reads, “He (Good Samaritan) went to him and bandaged his wounds, pouring on oil and wine.” Is there a medical basis for it? Are there clinical or biological studies or scientific journal on the matter?

Why would we want to increase drug permeability in a wound repair formulation when skin layers have been removed in a wound created in an animal model?

My question is how I can increase the quantity of RNA isolated from cells in wound edge in this case from keratinocytes? I have done the experiment and still the quantity of isolated RNA is low for RNA seq. does anyone have experience who could help me?

Best

What is the best way to image results form a wound scratch assay- I have an inverted scope with no camera and a confocal microscope. Any ideas would be appreciated!

I want to evaluate wound healing of a drug-delivery system in rats. According to the ethical standards for the use of animals in research, it is recommended to use a minimum number of animals to obtain scientifically valid results. This causes some researchers to make more wounds on an animal, and I did not find any study on its standards. For example, many studies use 8-millimeter round surgical wounds and some researchers made 12 wounds in each rat while some others made fewer ones, this raises questions:

1- What is the maximum number of wounds created in each animal (for example for 8 mm surgical wound model)?

2- What is the minimum distance between wounds so that the local effects of the two used drugs have the least effect on each other?

3- Is there any evidence comparing the effect of used drugs with systemic and non-systemic absorption in the distance between surgical wounds?

Best regards,

Can I make two separate wounds in the same animal and test, in each wound, a different concentration of my extract at the same time? wouldn't that give me false results of the activity?

Dear Researchers,

I need dear researchers to introduce a plant extract for scar Debridement of wounds or burns

thanks a lots

I have data from different wound healing experiments (Epithelial cells) where each experiment produces data that is at a different range than the data from other experiments. The effect seems to be consistent between controls and corresponding treatments each day but cannot be compared to other days because the ranges are so wide.

Everything is the same protocol wise, the only source of change that is probably causing the difference is the wound size. I have to use a tool to scratch the surface of the plate but it's difficult to produce consistent wound sizes. Especially since I'm working on the um scale. For example, some experiments have a wound size of 300 um and other experiments might have a wound that is 600 um.

Within single experiments there seems to be some statistical difference but I don't know how to compare experiments from one day to the experiments of another day.

Does anybody know how to normalize this data and/or do statistical analysis?

I have been studying using PRP for chronic ulcer treatment but I was wondering if it could be used for chronic wound healing. I have several patients in the institution I work at that present chronic wounds that due to their advanced age do not heal. As PRP could promote re-epithelization, ¿could you help me in finding some articles that support the use of PRP in wound healing?

I have herbal extracts obtained by maceration in 80% ethanol that are desired to be evaluated for their wound healing activity using excision and incision wound model on albino rats.

Although the extracts are prepared using the same solvent they have different solubility profile in water (some form clear solution while others results in a homogeneous suspension).

We intend to formulate the extracts into a semisolid dermal preparation using either aqueous cream base or simple ointment base as they are the most frequently used bases with the least intrinsic efficacy.

Is there a suitable method to measure and compare the release of the extracts from the aqueous cream base and the simple ointment base in order to evaluate their suitability.

I understand there will be some persister cells in the MRSA biofilm that are in dormant phase. I wound like to know if this kind of cells also exist in planktonic MRSA?

I have the dimensions of an electromagnet comprising a 0.8mm diameter copper wire, which is used to make a coils around 11mm X 13 mm rectangular cross section core material. The dimensions of the coil after wounding around the core is 25mm X 23mm cross section and 17.6 mm height.

So, how to find the effective number of turns in this for calculating M.M.F and also what other properties can be calcuated from this?

I understand that both sloughy wounds and necrotic wounds are composed of dead cells, but why necrotic tissue is black while sloughy tissue is yellow?

Currently looking at research related to early detection of wounds (e.g. pressure injuries) and projects managing risks (e.g. infection) in wounds using thermography.

I want to study the wound healing on an animal model using nanocomposite hydrogel. For a wound of 10 mm diameter, how much moisture is created that causes the wound dressing to swell and release the therapeutic agent? Usually in literature moisture content around 60-80% is reported for wound dressing.

I would be grateful for your help.

Many surgical unit has protocol to manage post surgical wound care and some of these wound undergo to be complicated as chronic to heal . I'm waiting for articles that help my project

Hi there!

In his 2014 masterpiece The Body Keeps the Score, van der Kolk claims that traumatized people may "try to cultivate an illusory sense of control in highly dangerous situations" in an attempt to master the physiological and psychological consequences of their trauma. Is there any research which shows that emergency workers (police officers in particular) have a higher incidence of trauma prior to joining the job? That is, is there any research which proves that trauma may be a motivator that pushes people to become emergency workers? I'm acquainted with the idea of the "wounded healer", but I'm interested in the scientific literature on the topic as it relates not to therapists but to emergency workers.

Thanks a lot.

Best,

Marc

I'm working on development of drug loaded lipid based topical carbopol based hydrogel for future treatment on diabetic wounds. It includes the characterization study regarding the former things and looking for a journal within impact 1-2. Kindly help me suggesting suitable journals.

Dear all,

I know that Uropathogenic Escherichia coli (UPEC) frequently has pathogenicity islands (PAIs), that contribute to their virulence. But I wounding if Avian pathogenic E.coli has the same islands or it is associated only with UPEC

Oxylipins play a huge number of roles in animals and plants – including cell-signalling, inflammation and wound response – but did they help ancient microbes ‘invent’ multicellularity? https://buff.ly/3wibP9I

I'm looking for a software to make panels for histopathology images and wound contraction images. Kindly suggest me a software which can import normal jpg/tiff images and make perfect sized images.

found in post CABG wound and very difficult to clear

Any guidelines for different types of wounds, tips for evidence-based practice for wound care will be greatly appreciated.

Thank you

I have a set of experiments trying to stop the activity or expansion of the rot produced with these bacteria

I'm DNP student that wants to discuss wound care discharge planning for my research. This is a very broad topic, and needing help to narrow the topic down.

Any ideas on wounds that need more discharge coverage?

There are many factors/barriers to discharging patients with wounds, but what is one area you think needs more focus?

Endogenous (self - burst) wound healing in patients with impaired metabolism, seeks for attention on many factors like tissue perfusion, peripheral neuropathy, tissue oxygenation, etc. whereas in induced diabetic models and excision wounds vascular and nerve supply are not compromised as same as in humans.

It is seen that other local and systemic factors like, hemoglobin, nutrition, limb activity, digestion etc. are not being focused in experimental studies.

In a nutshell, traumatic and atraumatic wounds, excision/incision and self burst wounds follows different healing methods.

The medicaments showing positive outcomes in excision models of diabetes induced Wistars should not be blindly followed without focusing on other healing factors.

Dear Researchers,

I need your opinion on, in order I want to do wound patch from plant extract, should the extract must be in powder or paste form? I had already dried the leaves, grind using grinder and macerate in methanol. Any idea>

hi everybody.

I'm using 75-85% deacetylated medium molecular weight chitosan.

and what if I want to load some wound healer medicine on it.

I am planning to perform a scratch wound healing assay with endothelial cells. So far, I don´t have experience with this technique. However, I would need to use a 96 well/plate to reduce the amount of inhibitors to use. What are the problems in case of manual scratch with pipette tip 100 µl of a 96 w/p? Does someone of you made experience in scratching 96 w/p? Would the use of a ruler or a line drawn on the back of the plate help me for making straight uniform scratches? thanks:)

Amikacin is a polar drug meant for parenteral use against gm negative bacteria.In some cases,different forms of amikacin meant for local application are used but can injection amikacin is effective if applied directly on wounds?

What effect would they have if applied on a wound? Antibodies, from another organism, applied on a wound, by the means of ointments and the like.

Does anyone have experience doing scratch assays in stem cells in 96 well plate using the wound maker? I notice that after doing the scratch and washing twice, the cells round up and there are gaps between cells although were confluent at baseline?

Wells are coated with matrigel.

when we apply the PRP on the wound the GFs will be released, why?

I am interested in pursuing my masters thesis research on the use of Tilapia skin grafts in wounds of dogs. Any inputs would be appreciated.

How to evaluate extraction socket healing (when the wound is primarily sutured)? Most of the studies use Landry and Turnbull Healing Index. There is also the H2O2 bubbling test. Do you have any other suggestions regarding this?

Thanks!

What is the role of local Carboxy therapy in chronic wound treatment?

What is the role of Ozone therapy in chronic wound treatment?

Hi, In our study we are going to evaluate the effect of 3 different treatments on burn wound using rat models. what should the P-value cutoff be For statistical analysis of our q-pcr output and collagen volume fraction results from image j?(0.01 or 0.05)

It is unlikely that I will get a specific answer to this question, so I propose to start a discussion on the geometry of the standard model.

The standard approach to this question is that, in addition to space-time, the space of internal symmetries of the Lagrangian is postulated, which determines the equation of motion of an elementary particle. In other words, in the standard approach, the invariance of the group action on the equation of motion is used as an additional internal space.

It would be interesting to know what kind of geometry is hidden behind the standard model of elementary particles. In this regard, let me share my thoughts on the geometric foundation of the Standard Model in this discussion.

The author's interpretation is as follows. Matter moves along the surface of a seven-dimensional sphere. At the same time, the vacuum form of matter is a Clifford torus S³xS³ with three-dimensional spheres moving in the process of evolution in circles so that the radius of one sphere increases and the radius of the corresponding circle decreases, and vice versa, the radius of the second sphere decreases, and the radius of the corresponding circle increases. As a result, the shape of the vacuum is the product of the Clifford torus and the time torus S¹xS¹. In this case, the Minkowski space-time (with the signature +1,-3) is wound on the product of a three-dimensional sphere of a large radius and a circle of a time torus of a small radius, and the additional (dual) Minkowski space (with the signature +3,-1) is wound on the product of a three-dimensional sphere of a small radius and a circle of a time torus of a large radius. Thus, a doublet of Minkowski spaces, which is an 8-dimensional space with a neutral metric is wound on the product of the Clifford torus and the time torus.

Within the framework of this model, elementary particles are associated with the minimum length closed lines of the product of the Clifford torus and the time torus, and the mass of elementary particles is associated with the pseudo-Euclidean length of the corresponding curve in 8-dimensional space with a neutral metric. Thus, massless particles lie on a compactified isotropic cone. And the most interesting thing in this geometric model is that the group U(1)xSU(2)xSU(3) naturally arises as the symmetry group of the compactified isotropic cone of the doublet of Minkowski spaces.

I have an ICU patient who is known to have ESBL-producing E. coli colonisation detected on numerous rectal swabs in prior admissions. Now, he has E. coli bacteremia with isolates not ESBL-producing. How is this possible? What could be the likely source of his bacteremia? He has no wounds. Thank you in advance for sharing your knowledge.

Maybe because the Minkowski space is actually wound on the manifold S²xT², just as a pseudo-Euclidean plane is wound on a torus by mapping isotropic straight lines to the defining circles of the torus.

I am looking for a best scaffold for cell delivery to the skin wound. I am wondering about acellular natural scaffold but nowadays synthetic scaffold specially made of polymer e.g PLGA, is really interesting. Could you please let me know your experience in these field?

53 Y Female was exposed to boiled water which fall on her abdomen. She left the burn untreated and scab formation occurred. TBSA expected 1-4% , probably superficial partial thickness burn. How would you manage this burn? Peel the scab and create a moist wound environment? Or what procedures would you follow now after the scab has formed?

Given parameters:

Stroke:+/-25 mm

Excitation: 5.3V rms, 2000 hz

Bobbin: SS tube, 3.5 mm ID, 4.65 mm OD

Primary coil is wound over the entire length of bobbin

Two secondary coils are wound over primary -- one in each half section.

Sum of two secondary outputs: 3.72 V rms

Determine:

Length of bobbin, number of turns of primary and secondary coils

I am to study the wound healing of infected wound in animal model. However I am not sure about the animal model to be used. Whether swiss albino mice or Wister albino rats or Sprague Dawley rats would be appropriate for the study that involves an infected wounds on the mice/rats. .

I am looking for ways in which I can compare wound healing among different treatment groups

We have evaluated the wound healing potentials of a compound at a particular concentration in ointment form in laboratory animals. Now, we are preparing different types of topical formulations (gel, ointment, spray etc) for wound healing studies on clinical wounds of domestic/livestock animals. Does it require to test each topical formulation again on laboratory animals before proceeding for study on clinical wounds?. Please, also tell me that what should be the other guidelines which I have to follow before going for study of our topical formulations on clinical wounds of livestock animals (sheep, goat, cattle etc) for the drug development process.

This question is for wounds surgeons and consultants working in area of wound practice and research

Hi everyone. I have recently observed that after I made the wound and switch the medium, in some wells the cells look like dead.

Dear all,

I came across your paper 'Fully convolutional networks for diabetic foot ulcer segmentation' because we are working on wound segmentation and I was wondering if you can share the images of foot ulcers and theirs ground truth images, so we would be able to carry out real experiments and compare it with other implementations. We appreciate your collaboration and your work will be cited in our possible outcomes.

Thank you very much,

Karl.

On your web pages there is an article by Telichowska KS with bibliographic data of the Integrative Molecular Medicine (IMM) journal (Telichowska KS (2019) Wound coverage by linen dressing accelerate ulcer healing. Volume 6: 1-3. Integr Mol Med, 2019 doi: 10.15761/IMM.1000371)). Unfortunately, there is no such article on the IMM's page. The article was withdrawn a few months ago from OAtext publisher pageges, remove it from your pages.

Jan Szopa, professor of biochemistry University of Wroclaw

What are some biomarkers that can best describe the wound inflammatory phase during the wound-healing experiment in rats?

Good day,

I will really appreciate your brilliant minds on the above question.

I am working on developing a chronic wound in vitro model, specifically diabetic foot ulcer. I am looking for an effective way of rendering the choice cells hyperglycemic, to mimic diabetic conditions. Any useful idea please?

Thanks a lot,

Mirella

The wound myiasis causing flies of animals are potential human myiasis agents. Monitoring the animal myiasis helps to more precisely identify the dominant and aggressive species in the province.

I am working on subject called "Father wound" in psychology. I would like to hear from people who work(ed) on the same subject.

Thanks a lot

Dear all,

We want to model a pressure vessel using WCM (Wound Composite Modeler). We have built a model  by following  the instructions in user manual and examples. For post processing, UVARM subroutine was generated by WCM Plug-in. Then we have run our input file with UVARM subroutine; however, we have encountered an error as expressed below;

" Abaqus Error: The executable Standard with system error aborted with system error "illegal memory reference". (signal 11). please check the .dat, .msg, and .sta files for error messages if the files exist." 

Since we have no internet connection at our computer where input files are run, we can not generate system information to report. When we search the error on the internet, common offer for solving problem is avoiding parallelization but we have tried it with one cpu and we have encountered with same problem again. 

By the way, to compile and run our model, we made some changes in UVARM subroutine file. It is that logical definitions were changed into integer definitions (e.g. false->0 and true->1) because we use gfortran as compiler.

Could you please inform us about the possible solutions to our problem? We're looking forward to your reply. Thank you in advance.

Best Regards,

Phenytoin is known to increase the fibrotic growth of ginigiva when administered in therapeutic doses in human . Is it expected to facilitate fibrosis in wound area during healing

The alpha tocopherol is sold as an ointment while the aloe vera is sold as powder. I am concerned with making the final volume too bulky for topical application

can treat acute burn wound with amniotic membrane graft ?

I'm looking for the typical values for Mass transfer k used in the calculation of concentration in membrane wall (cases of spiral wound and flat membranes)

I need your precious suggestions,

How may i carry out an experiment, The use of nano-medicine for burned wounds. .

In ancient Greece, hoplite gained Kleos, glory, by dying in battle. If they were so injured that they could no longer fight, they would not be named on their tomb, therefore gaining no kleos. What kind of reception did they have when they returned to the polis, alive but no more fighter? Did the psyche of the particular polis affect this reception? Comparing the democracy of Athens with the military camp mentality of Sparta.