Questions related to Wound Healing
I am currently searching for a topic to write my thesis on but to no avail. Preferably I would like the argument to be centered around plastics and reconstructive surgery since it is a specialty I would like to pursue after medical school.
The topics that interest me the most in plastic and reconstructive surgery are :
- Wound healing
- Vascularised Composite grafts
- Craniofacial surgery
Regarding these topics, I would like to know what within these arguments are trending questions.
i encapsulated BSA-stabilized curcumin NP in the hydrogel matrix, and for antibacterial test i immersed it in 75% ethanol solution for 2 hours (referred in the lieratures), followed by washing with PBS buffer for 3 times. however, after alcohol sterilization i found alcohol color changed into light brown implying that BSA-Curcumin NP was extracted. actually, it is feasible i think.
So i am afraid this loss of encapsulated materials in the hydrogel will definitely affect its antibacterial, and further wound healing action. Then, which method is preferred for above type hydrogel? its nearly impossible to synthesize all polymer components aseptically even though hydrogel fabrication can be done aseptically. please let me get out from this delima. thanks!
I have data from different wound healing experiments (Epithelial cells) where each experiment produces data that is at a different range than the data from other experiments. The effect seems to be consistent between controls and corresponding treatments each day but cannot be compared to other days because the ranges are so wide.
Everything is the same protocol wise, the only source of change that is probably causing the difference is the wound size. I have to use a tool to scratch the surface of the plate but it's difficult to produce consistent wound sizes. Especially since I'm working on the um scale. For example, some experiments have a wound size of 300 um and other experiments might have a wound that is 600 um.
Within single experiments there seems to be some statistical difference but I don't know how to compare experiments from one day to the experiments of another day.
Does anybody know how to normalize this data and/or do statistical analysis?
Post burn wound healing cosmotic appearance its relation direction of debridement of burned tissues and the the effect on body image of patient as well as quality if life so i am in need to understand the relation the debridement with post burn changes in cosmotic appearance of patient
I have loaded 2 compounds and a nanoparticle to a cream base. I need to study the drug release of the compounds and nanoparticles individually. Please suggest me a better technique/technology for carring out timely drug release.
I have been studying using PRP for chronic ulcer treatment but I was wondering if it could be used for chronic wound healing. I have several patients in the institution I work at that present chronic wounds that due to their advanced age do not heal. As PRP could promote re-epithelization, ¿could you help me in finding some articles that support the use of PRP in wound healing?
hello. I am a student studying MTT assay and Wound healing.
Studying MTT and Wound healing, I learned that MTT measures cell viability and Wound healing measures cell proliferation rate and inhibition of migration.
However, I thought that it could be said that cell migration was inhibited by the inhibition of cell viability.
Can you tell me the exact difference between MTT and Wound healing?
Or do you have a paper to refer to when studying the difference between MTT and Wound healing?
I have herbal extracts obtained by maceration in 80% ethanol that are desired to be evaluated for their wound healing activity using excision and incision wound model on albino rats.
Although the extracts are prepared using the same solvent they have different solubility profile in water (some form clear solution while others results in a homogeneous suspension).
We intend to formulate the extracts into a semisolid dermal preparation using either aqueous cream base or simple ointment base as they are the most frequently used bases with the least intrinsic efficacy.
Is there a suitable method to measure and compare the release of the extracts from the aqueous cream base and the simple ointment base in order to evaluate their suitability.
I will implement indirect co-culture by using transwell inserts. Macrophages will be in the inserts and lung cancer will be in the lower well. We will do a wound-healing assay for A549 during co-culture. What is the recommended pore size and number of inserts to do the assay? Are the characteristics of inserts that will be used for wound healing is same for western blot? if not what would be?
I went through few papers, in so,me they are saying that acidic pH help in wound healing and in some they are saying that neutral to alkaline is better for wound healing. So I am confused. It would be great if I can get some clarity on this.
While going through some literature I came across chemical signals such as Growth Factors (EGF/ FGF etc) released during wound healing. Are there any more such stimuli one can use to trigger drug release from a polymeric scaffold?
I use indirect MTT test to investigate the effect of the biomaterials I have developed. For this, I use the filtered extracts of my materials that have been kept in the medium in an incubator for 24 hours. As vehicle control, I also put the standard medium (containing 10% FBS, 1% Pen/Strep) into the incubator.
The vehicle control medium delays the closure in my scratch test for wound healing while increasing viability in MTT (significantly compared to the control medium that I did not keep in the incubator).
What factors in the medium can we connect the effect seen in these two tests? I attribute the delay in closing the scratch test to the serum, but I could not understand the increase in viability (we can say a kind of increase in mitochondrial activity) in MTT. Could it be pH-related?
Thank you very much.
I have prepared and hydrogel via freeze thaw method and then I performed its physical characterization like swelling, water content, moisture retention, gel content etc. Before conducting these experiments first I dried my hydrogels. I want to know it is the right way to perform the analysis? I have read the literature and it is mentioned that for these experiments hydrogels are dried but in few studies they have not mentioned whether they have processed the samples before these experiments.
I would appreciate the response
in passage 0 the cells grown properly but after subculturing they did not adhere to the flask and showed cell death(round in shape and less number).Some of the cells that are frozen in -80 degree was revived in T75 flask the load found to be too low and no proper cell growth, cells are found to be round in shape and floating. What may be the reason? Kindly suggest me the possible reason for the above mentioned problem.
I am working on a wound healing activity. I want to develop a wound healing formulation for which I am looking for a specific and standard formula. Can anybody help me for this with a standardized formula?
A helping hand would be greatly appreciated.
I am developing plant extract incorporated electrospun nanofibers for biological applications
Please discuss your ideas regrding which type of extract could I use for taking plant extract and criteria of polymer for the purpose
Please share your valuable experiences
I have a set of data from wounds that are going through normal wound healing and those that have been treated to intentionally delay wound healing (n=3) in pigs. For normal/acute wounds, tissues were harvest at two timepoints: Day 5 and Day 10 from the point of wounding. For wounds with delayed healing, four timepoints were collected: Day 5, 10, 15 and 20 from the point of wounding. These wounds are all on the same pig and repeated twice on two other pigs. I would like to answer the question: Is wound healing significantly delayed in the treated wounds compared to acute wounds.
Initially, I just used an ANOVA to compare across all wounds and all timepoints but I'm not sure if this is the appropriate test to use. Should I be doing a pairwise comparison for each timepoint (eg. Acute D5 V Treated D5) instead of analyzing all the timepoints together (as acute does not have timepoints day 15 and day 20).
we are keen to evaluate certain molecules for their potential to accelerate wound healing process in in vitro set up. Two cell culture models i.e 3T3 and hPBMCs we are using. I would like to know if some other cell models that can support the testing.
I'm searching for a research article for cytokine study by ELISA for IL-6, TNF-a, IL-1B. Kindly recommend some good papers to proceed.
I am doing wound healing experiments and found some substances that seem to enhance the proliferation of skin cells and might be even synergistic when applied together.
I would like to calculate it with CompuSyn by Ting-Chao Chou (a software making researchers lifes so much easier, thank you for that!!)
I cannot enter effects > 1.0, thats why I normalized the control to 0.75 so that all values are < 1.0.
Is this thought too simple? Does anybody have another suggestion?
Thanks in advance,
The lady 72-years old, 10 days after anterior resection because of rectal cancer (T3N0M0) developed anastomotic leak accompanied by surgical site infection. Since she has "frozen abdomen" we did not perform diverting ileostomy. We placed EndoSponge and abdominal vacuum assisted abdominal closure with quite good abdominal wound healing.
Is EndoSponge placed properly? It was not possible to place it deeper in the anastomotic cavity. The negative pressure works.
Do you usually allow the patients to consume liquid diet during EndoSponge treatment?
Thanks for your help
Endogenous (self - burst) wound healing in patients with impaired metabolism, seeks for attention on many factors like tissue perfusion, peripheral neuropathy, tissue oxygenation, etc. whereas in induced diabetic models and excision wounds vascular and nerve supply are not compromised as same as in humans.
It is seen that other local and systemic factors like, hemoglobin, nutrition, limb activity, digestion etc. are not being focused in experimental studies.
In a nutshell, traumatic and atraumatic wounds, excision/incision and self burst wounds follows different healing methods.
The medicaments showing positive outcomes in excision models of diabetes induced Wistars should not be blindly followed without focusing on other healing factors.
I'm using 75-85% deacetylated medium molecular weight chitosan.
and what if I want to load some wound healer medicine on it.
Hello, I am new to the ImageJ and basic science world. I am working on a project that where we are measuring wound healing in B2-spectrin endothelial specific knockout mice. I have IHC images (below) where I have stained for CD-31 (red), and I would like to quantify the immunofluorescence of these images. I have found various tutorials online that discuss mostly quantizing individual cells. But I am more interested in the whole area, as this is essentially measuring the vascularity of the wound bed. The image attached is the combined channels but I can split the image into the respective channels for analysis.
So far, I have really only done the "analyze -> measure" feature and getting the mean gray value. I am I at least on the right track? Any tips? Thanks!
Real time PCR primers given in the research article can be used for different species of rats for monitoring wound healing?
I have been trying to do wound healing assay on Human Corneal Epithelial Cells with 2-well ibidi insert. However during media change with Mitomycin-C and washing with HBSS (after removing insert) steps some parts of HCEC monolayer keeps detaching away from the well plate.
My simplified protocol is:
Seed each insert with 70ul of cell suspension (density is 4x10^5 cells/ml)
Incubated around 12-16 hours.
Then changed media with Mitomycin-C and after 1 hour removed insert, and washed with HBSS. and added Media. Then take time lapsed images.
Anyone, who did work with HCEC have any suggestions about how keep HCEC monolayer intact?
Can I use HT1080 (fibrosarcoma) cell line instead of the normal fibroblast cell line (Human dermal fibroblast, human keratinocytes) for wound healing studies?
Where can I find some information regarding sterility requirements for topical dosage forms?
To the best of my knowledge sterile topical drugs are: ophtalmic drugs, for wound healing and burns, gels used in endoscopy (anesthetic mostly) and certain dermoscometics?
Are there other type of drugs that should be manufactured in sterile dosage forms? (only topical).
Thanks very much!
I have a Markov model from a colleague for wound healing. 4 stages open infcetd closed and deathd death. The paper used to inform the transition data has monthly healing rate over 6 months and an overall infection rate within those 6 months.
My colleague used the month 1 healing data for the transition probability and calculated a monthly probability for infection. I do not underttand why the firtst months healing data was suitable rather than calculating from using all of the healing data at months 1,2,3,4,5,and 6.
My colleague is no longer here and no-one else can explain. I am concerned our out come is wrong due to wrong data being used. we are using a time horison of 5 and then 10 years.
thanks for any advice
I will use 6 well plates. I'm studying the migration of the macrophages? Is it possible cell be migrate after it cell already differentiate monocytes into macrophages?
I have been working with wound healing and I am facing problems as I am unable to find normal murine dermal fibroblast cell line to study the wound healing effect. The dermal fibroblast cell lines present in NCCS repository are melanoma cell lines. It would be kind, if I would get to know whether there are normal murine fibroblast cell lines in any of the repository concerned. The information regarding such cell lines will be highly appreciated.
I am trying to measure the area of my scratch for wound healing using ImageJ. I have a large amount of images, so tracing the scratch manually is not a good option. I have tried MRI_Wound_healing_tool and I get many areas outside of the scratch selected.
I am working with lymphatic endothelial cells and trying to test effect of few chemicals, on LECs by wound healing scratch assay. My confusion is, if I have 4 different chemicals cited in different literature to be used for cell culture studies, in different concentration let's say, if A is 10 um then B 25, C may be 100 and D 400um. What would be ideal concentration if I want to compare the effect of these individual chemical on one cell type. Should I be choosing one from literature individually or a standard concentration for all to be used, to make a comparison.
Aloe vera exhibits a range of therapeutic activities such as anti-microbial, antiviral, anti-cancer, anti-oxidant, anti-inflammation, skin protection, wound healing properties as well as the regulation of blood glucose and cholesterol. I want to know exactly what aloe vera medications are used for.
We have evaluated the wound healing potentials of different compounds at particular concentrations in laboratory animals. Now, we want to move in the direction of drug development for some topical products for wound healing. So, we require the complete guidelines which should be followed and fulfilled for further study in the direction of drug development for topical formulations for wound healing.
I am to study the wound healing of infected wound in animal model. However I am not sure about the animal model to be used. Whether swiss albino mice or Wister albino rats or Sprague Dawley rats would be appropriate for the study that involves an infected wounds on the mice/rats. .
I am looking for ways in which I can compare wound healing among different treatment groups
Can anybody suggest me one/more plant names for doing project either in alone or combination therapy of diabetic mellitus, wound healing and anti oxidant property?
Currently I use U-2OS for transient overexpression of genes of interest. Using Lipofectamine 3000 is quite efficient for overexpression and verified by WB. However, recently I tried to combine overexpression with scratch wound healing assay and found that groups transfected with empty vector (pCMV3) as well as expression vectors all grew slower than non-treated and Lipo-only groups. This rules out the toxicity of Lipofectamine 3000 to cells.
Does anyone have similar experience?
Transfection of simply plasmid hurts cells?
Should I reduce the amounts of DNA?
or merely lengthen time span of my observation? coz cells grow slower but not die out.
We have evaluated the wound healing potentials of a compound at a particular concentration in ointment form in laboratory animals. Now, we are preparing different types of topical formulations (gel, ointment, spray etc) for wound healing studies on clinical wounds of domestic/livestock animals. Does it require to test each topical formulation again on laboratory animals before proceeding for study on clinical wounds?. Please, also tell me that what should be the other guidelines which I have to follow before going for study of our topical formulations on clinical wounds of livestock animals (sheep, goat, cattle etc) for the drug development process.
We have now tried a number of commercially available antibodies to detect the receptor of AGEs (RAGE, sometimes called AGER) in parrafin embedded mouse tissue with DAB staining. As a control we have used corresponding tissues of a RAGE-KO mouse. Whereas most of these antibodies worked well in lung tissue that accumulates RAGE to a high amount, we have faced significant problems when using tissues that express only minor amounts of RAGE. In particular we observed cross reactivity with muscle cells. If anybody has had good results using an anti RAGE antibody for IHC in mouse tissue please let us know!
I need to know the process as well as a legit article/journal/literature. Any Ideas or suggestion would like to recommend. Thank you, kind sir and ma'am.
I'm working on breast cancer surgery, and I'm going to estimate wound healing process time in different groups after breast cancer surgery. Here I want to know the parameters that are required to estimate the time. Based on which parameters we can able to confirm the healing time as delayed.
Phenytoin is known to increase the fibrotic growth of ginigiva when administered in therapeutic doses in human . Is it expected to facilitate fibrosis in wound area during healing
I am testing some compounds for wound healing in rats (rodent) and want to further extend the research for the drug development for wound healing in animals/human. So, please tell me the different parameters (as a toxicological data), which I should to do in rodent before proceeding to experimentation in large animal species. At present, I am using the compounds topically alone and in nanoformulation form.
I have a collaborator who is looking to investigate wound healing in some tissue samples using IHC, and it's outside my area of expertise. Any common markers you can recommend?
i want to find swelling degree of cross linked films.i need to know whether any relation is there between swelling degree and biodegradability of films.Actually what is the real purpose of doing sweeling degree of films or water uptake of crosslinked polymer.what idea we get from doing this? i know for wound healing application sweeling degree of polymer should be between particular value. any other purpose is there to find sweeling degree of crosslinked fillms?plz answer this question?
so i want to evaluate the effect of a topical gel of a plant extract for wound healing, but still didnt know how to determine the frequency of dosing, because some article i read said its given daily, the other said every 2 day, an another said every 3 day
*files below are not mine
How to stain Myofibroblasts in myocardial infarcted hearts? What about Alpha- Smooth muscle actin in the detection of myofibroblasts? Is there any other specific markers for the detection?
I'm working within a project on the role that hyaluronic acid (HA) in tumor cell migration and invasion. I have started testing low molecular weight oligosaccharides (maximum 18 repeat units), but these compounds seem to have little effect in our models. For this reason, I would like to try some type of higher molecular weight HA (HMWHA), but I'm not sure about which MW size(s) should I try. Also, I don't know how these HMWHA would be used in an in vitro cell migration/invasion system (soluble? gelified? wound healing or transwell?), so any help in these will be really appreciated.
Can you suggest few natural compounds having wound healing activity and can be used for in vitro wound healing activity ?
Hello! I want to prepare scaffolds for tissue engineering. For in vivo tests I need to preserve the wound dressing from drying. Can you provide some nice idea with coating or additional layer deposition for protection from drying? Any good practical experience?
I am interested to assess the skin permeation potential of some formulation prepared for topical use for wound healing or other problem of skin. For this study, I want to use franz diffusion cell assembly for assessing in vitro permeation of the drug present in formulation. So, please provide me the details of manufacturer/ company which provides good quality franz diffusion cell for research purposes.
The silver nanoparticle has a high toxicity?
we know it has very good antibacterial effect but how it can good agent for wound healing despite its toxicity effect? has a negatively effect?
Currently Importing Ob/Ob Mice is a challenge for me, I'm looking for a mice strains which mimics Diabetic foot ulcer. I'm researching on Mildly impaired Wound Healing and Mild Neuropathy.
I tried to use the institution database to find the article but unfortunately failed. The article needed for animal ethics approval form my institution. I would be grateful if you can provide me a copy. Thank you
I plan to prepare a natural wound healing cream using plant extracts as its active ingredients. A preliminary study showed that 300mg/kg (from in vivo study using rats weighing between between 180g to 220g) of plant extracts are very significant in healing the wound. Now, I am so confuse on how can I conduct a Total Microbial Count (TMC) by applying this 300mg/kg of extract.
Is that the concentration of extract from the preliminary study (300mg/kg) must be applied in development of wound healing cream? or the production of cream can use totally different concentrations/percentage ratios of extracts depending on the formulation?
Looking forward for experts particularly the ones in product development.
Thanks in advance.
I want to evaluate Antidiabetic and wound healing activities on the same time on rabbit. Can I do it ? What about ethic is it acceptable.
Patients affected by epidermolysis bullosa (EB) , a rare genetic connective tissue disorder with symptom of extremely fragile skin that blisters and tears from minor friction or trauma , are suffering from many problems . EB is always painful, often pervasive and debilitating, and is in some cases lethal before the age of 30 .Internal organs and bodily systems can also be seriously affected .
Daily wound care, pain management, and protective bandaging are the only options available for people with EB.
Although there is not a real cure for EB , I want to start researching in order to improve their quality of life and reduce their pain and problems .
So, I wounder if I could be guided by your answer what is most important issue ?
Hello there, the experiment I performed is a scratch wound assay on Hela43 cells transfected differently: a non-transfected control, emptyGFP, Cx43D3N, Cx43G138R, wildtype Cx43 and a non-transfected with Gap27 (positive control). the cells were photographed in 4 different time points, then the wound area was measured over time. I want to determine if there is a significant difference in the wound area between the different cell types, ie. if the mutants have any effects on the rate of wound healing at all.
ps if possible, please include as much information as you can about how to carry the test out on prism, I'm an undergraduate student and it's the first time I'm carrying out a statistical test like this!
thank you very much!!
Both cell proliferation and migration contribute to reepithelization of wounded skin. To observe the effects of any genes or compounds on cell migration, we usually use the scratch assay to assess the keratinocyte motility, and regard it as a measure for the cell migration in vivo. Normally, in the scratch assay we treat the cells with mitomycin c to block the cells' proliferation. Can we treat mice with any small molecular inhibitors to block the proliferation of keratinocytes so that we may assess the cell migration in vivo? If this is not possible, are there any other methods that can be applied for this purpose?