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Humor in Journalism and Reporting
Journalists often allude to children’s literature, because in our diverse culture, memories of classic children’s books are what we all have in common.
Nursery Rhymes and Folk Tales are a rich resource because they present a full array of personalities
Chicken Little to represent alarmists
Pinocchio to represent liars.
The Big Bad Wolf to warn us of danger
The Frog Prince to give hope to discouraged women
Humpty Dumpty to point out how easy it is to fall from grace
In the Old Days, when Humpty Dumpty fell, sympathetic bystanders rushed to put him back together, but in a recent cartoon he was shunned by creatures shouting “Salmonella!”
Cultural Icons can be either
recognized visual symbols
or familiar words that can be parodied.
Editorial Cartoonists first have to help the viewers into the mindset of the original,
Then take them in a new direction.
For efficiency, cartoonists make use of common visual symbols
Pointing fingers or arrows
The Trojan Horse
Tombstones and the initials R.I.P.
Skulls/The Grim Reaper
The three monkeys (See no evil. Hear No evil. Speak no evil)
The Ghost of Christmas Past
Superman
Railroad tracks not matching up
What are the cultural icons used by American journalists and editorial cartoonists? What about the cultural icons of other countries?
Don and Alleen Nilsen “Humor Across the Academic Disciplines” PowerPoints: https://www.public.asu.edu/~dnilsen/
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I'm interested in conducting TLC analysis for pheromones, but the quantity available is quite small. Does anyone have experience analyzing such small quantities of pheromones using TLC? Any advice or guidance would be greatly appreciated. What is the minimum quantity of a compound that can be detected using Thin Layer Chromatography (TLC)? Can compounds be visualized in quantities as minuscule as nanograms (ng) or picograms (pg) when employing TLC?
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It depends on the substance being tested. For example, 10 microliters of 1 mg/ml of flavonoids can be seen clearly, but 10 mg/ml of terpenes that need to be colored can hardly be seen clearly.
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Recently I have been trying to understand the effect of silica gel pore size on a mechanochemical reaction. It involves intense milling of spherical silica gel of similar particle size distribution but different pore sizes (6 nm, 10 nm, 30 nm and 50 nm, as indicated by the manufacturer).
I was told that silica with larger pore sizes would break more easily than those with smaller pore sizes when being milled, which makes sense because they would have more empty spaces inside the structure to make them more fragile.
Upon milling, the silica broke into different sizes; some are large, and some are extremely small and become a cluster of fine particles. However, it seems that not even TEM is able to visualize the pores this small. Is there any method that can actually visualize the structure of pores?
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You might try high resolution micro-x-ray tomography? It will produce A 3D image allowing 3D measurements, great detail.
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I am currently writing up a GT study for my PhD and have been writing memos throughout the study. They are in various forms (written, visual, recorded, sticky notes etc.). My question is that they are obviously part of the analytical process and I have included this in my methods discussion, but can they also be part of my findings section as an alternative to verbatim quotations? I'm not sure if I'm missing the point of memoing!
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In general, memos are part of the ongoing analysis process, rather than the results. The classic approach to reporting results in GT is to develop a set of conceptual categories and go through them one by one, with supportive quotations for each.
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 What specific changes in set design have digital technologies brought about?
 How does visual presentation in cinema influence literary narrative and theatrical atmosphere?
 Has the perception of classical literature changed in the context of modern technologies? Has it given new aesthetic meanings to works?
 Does multimedia make the traditional stage more attractive? How do classical works (for example, Chekhov or Tolstoy) combine with digital technologies on the modern stage?
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What specific changes in set design have digital technologies brought about?
Film is a medium that aims to create visual credibility. Scenes in films originate from imagination brought to life through technology, making them appear realistic. Thus, the term "realistic" refers to a lifelike quality that allows the audience to engage emotionally. Spectacular set designs naturally evoke and influence the emotions of viewers. Therefore, technology inevitably brings changes to set design, making it increasingly realistic. I hope this answer helps guide you in your research. If it does not fully align with your needs, I sincerely apologize.
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The provisional title of my PhD research proposal:
Artistic Exploration and Visual Communication of Megalithic Statues of Lore Lindu National Park
Background:
Lore Lindu National Park in Central Sulawesi is one of the important sites that holds a unique collection of megalithic statues that are rich in historical, cultural, and aesthetic values. However, the artistic potential and visual communication value of these statues have not been explored in depth in a contemporary design context. This research aims to integrate the visual and artistic elements of megalithic statues into a visual communication design that can connect this cultural heritage with modern society.
Research Objectives:
Assess the artistic, symbolic, and historical values of megalithic statues in Lore Lindu National Park.
To explore the potential of transforming the visual elements of megalithic statues into visual communication design.
Develop a design model or visual work based on megalithic statues to promote and preserve the cultural heritage of Lore Lindu.
Research Methods:
Artistic and Explorative Approach: Visual ethnography is used to document and analyze the artistic elements of the statues.
Design Experimentation: Adapting the visual elements of the statues into modern design media such as illustration, branding, and digital media.
Local Collaboration: Involving local communities in understanding the cultural meaning behind the statues as a basis for artistic exploration.
Expected Outcome:
A portfolio of visual design works that interpret megalithic statues as part of cultural heritage.
A scientific publication on the relevance of visual communication design in the preservation of megalithic culture.
Recommendations for design-based cultural promotion strategies in the Lore Lindu region.
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Sigied Himawan Yudhanto you are most Welcomed
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Dear researchers,
Recently reviewers frequently asking to provide graphical abstracts from authors. While researchers are not professional designers, and creating a graphical abstract typically doesn’t demand much design skills or significant time investment. But still, it seems excessive to me! If researchers choose to provide one, that's ok, it's up to them.
However, is it truly essential to visually represent every study? I believe reviewers shouldn't be overly strict about this requirement. I'm curious to hear other researchers' perspectives, reasoning, and justifications.
Regards
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Challenges for Researchers
Your concern about the burden graphical abstracts place on researchers, who are often not professional designers, is also justified:
  1. Lack of Training: Many researchers lack the design skills needed to create effective and visually appealing graphical abstracts.
  2. Time Constraints: While graphical abstracts might not require extensive time investment, creating one can still add to an already heavy workload.
Should They Be Mandatory?
The question of whether graphical abstracts are "essential" depends on the nature of the study:
  • For highly technical or niche research, a graphical abstract might not significantly aid comprehension or reach.
  • For interdisciplinary or widely impactful studies, they can be an effective tool to communicate findings to a broader audience.
Reasonable Flexibility
Instead of making graphical abstracts mandatory, journals and reviewers could adopt a more flexible approach:
  • Optional Submission: Authors should have the choice to include a graphical abstract based on the relevance to their work.
  • Guidelines and Support: Journals could provide templates or basic training to help researchers create effective visuals without professional design expertise.
  • Reviewer Sensitivity: Reviewers should recognize the primary focus of a paper is its scientific merit, not necessarily the aesthetic quality of supplementary materials.
In conclusion, while graphical abstracts have undeniable benefits, they should not overshadow the core scientific contribution of a study. A balanced approach that considers the needs and constraints of researchers is essential to maintaining fairness in academic publishing.
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Has the World Health Organization defined foundations or criteria for determining visual pollution, or are there other international standards?
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Dear Doctor
[Frameworks for measurement have been established and include public opinion polling and surveys, visual comparison, spatial metrics, and ethnographic work.
Visual pollution can manifest across levels of analysis, from micro instances that effect the individual to macro issues that impact society as a whole. Instances of visual pollution can take the form of plastic bags stuck in trees, advertisements with contrasting colors and content, which create an oversaturation of anthropogenic visual information within a landscape, to community-wide impacts of overcrowding, overhead power lines, or congestion. Poor urban planning and irregular built-up environments contrast with natural spaces, creating alienating landscapes. Using Pakistan as a case study, a detailed analysis of all visual pollution objects was published in 2022.]
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Dear experts it may concren,
We want to use stereotaxic injunction technique give AAV for up-regulating a protein in whole cerebella in P0 mice. Firstly, we have to try different injection locations and volumes for whole cerebella being infected. Normally, we should wait 4w to see the virus expression. Do you know someways to visualize AAV spreading area in 12h or 24h for saving time and reducing unnecessary harm to more mice ?
Thank you!
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Thank you, again. Winda Ariyani
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A visual artist is someone who practices arts such as drawing, painting, textile design, ceramics, graphic design, sculpture, Fashion design and the likes
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yes i am
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Hello
I created two spectral density plots in EEGLAB from the same participant's data. One corresponds to a resting-state control condition, and the other to a meditation condition. Both recordings were 5 minutes long with eyes closed, and they underwent identical pre-processing steps, which included:
1. Manual rejection of bad channels and major artifacts (e.g., movements).
2. Filtering (0.5–75 Hz).
3. Re-referencing to the averaged signal.
4. Independent Component Analysis (ICA) decomposition.
5. Rejection of artifact components using EEGLAB’s default flagging function, followed by visual inspection.
The control spectrum was processed on Computer A, which has the Signal Processing and Statistics and Machine Learning toolboxes installed, while the meditation spectrum was processed on Computer B, which does not have these toolboxes.
As expected, the two spectra are similar in shape, but their power density scales differ drastically. Specifically:
- The control condition processed on Computer A shows a shift down in power density, with some values even being negative (which is physically implausible).
- This discrepancy is not due to the different conditions, as it is not observed in other participants.
The issue seems to arise from differences between the two computers or the toolboxes available. Has anyone encountered a similar issue or can provide insight into the potential cause of this problem?
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Dear Suddha Sourav thank you so much for your help!
I think I found the origin of the problem. As indicated here https://github.com/sccn/eeglab/blob/develop/functions/sigprocfunc/spectopo.m the function spectopo.m is used by default if the signal processing toolbox is installed and returns the power spectral density. Otherwise the function spec.m is used, the output of which is the power spectrum and not the PSD. The units are then different but the topography should be similar. See also https://github.com/sccn/eeglab/issues/172
Thanks again!
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Dear All,
I hope that you are fine.
Please, who could help us? We need a tool for Galois Lattice Visualization (open source). For example we could navigate by query to find and visualize a given formal concept in the lattice and a number of hierarchcal level related to this concept.
Best regards.
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Dear professor Gammoudi,
Did you get any answer about the tool for Galois Lattice Visualization?
I'm looking too to a tool for Galois Lattice in my research work.
Best regards
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Hello,
I have several .xyz files (each composed of several molecules) and I'd like to find an automated way to visualize and save them.
I'd appreciate any guidance.
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Hi Ashly,
Have you found a way to do this?
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Hi,
I am a beginner in bioinformatics. I have more than 500 genomes to analyze and I already got the results from Roary. I want to visualize these results by showing the tree compared to a matrix with the presence and absence of core and accessory genes. Roary provides us with roary_plots.py, which can achieve this. However, If I have hundreds of genomes, the plot will be a mess and nor very clear.
If there are any tips to make the tree look more clear in the cases of having hundreds of genomes, I'd be grateful to hear them.
Best,
Lingyu
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Hello Lingyu,
I'm not sure if there is an optimal way to do it with roary_plots.py, since hundreds of genomes will still be many rows. Looking at the code of roary_plots.py, you should be able to increase the width of the tree to improve the clarity by changing the “colspan” parameter in the tree display. You can also improve the size and resolution of the image with the “figsize” and “dpi” parameters.
Best regards,
Adrian
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Dear colleagues,
I’ve created a video on YouTube simulating diffraction phenomena and illustrating how it differs from wave interference.
I hope this visual approach offers a clear perspective on the distinctions between these effects.
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nterference happens when two light waves meet and mix together. It is caused by two or more light waves coming together. Diffraction happens when a light wave bends around corners or through small openings. It is caused by light waves hitting an obstacle or passing through a small gap.
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Of course, we need a large, complete overall map.
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It seems that navigation doesn't really require locating the camera itself,
but real-time map-construction needs to know the camera's position.
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I am interested in the study of visual subcompetence in education, specifically how visual tools and technologies can be integrated into the educational process to enhance the development of professional competencies in future teachers, particularly in mathematics education.
I am looking for research and definitions that highlight and specify the concept of visual subcompetence in education. Specifically, I am interested in how visual subcompetence is distinguished as part of the broader professional competence, particularly in the context of mathematics teacher education.
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To think more number of case studies regarding visual subcompetence in education.
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How do we typically choose between Convolutional Networks and Visual Language Models when it comes to Supervised Learning tasks for Images and Videos ?
What are the design consideration we need to make ?
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Dear Titas De ,
CNNs can be trained and deployed more efficiently than VLMs, especially for large-scale datasets. This is due to their simpler architecture and the ability to leverage parallel processing hardware for faster computations.
Regards,
Shafagat
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I would like to know ho the grain grows a technical detailed explanation with a visual
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Practicality all engineering metallic alloys (except for a few special materials such as single crystalline superalloys) are in a polycrystalline state. This means that each crystal is defined in terms of its crystallographic orientation, its size and local topological environment and the properties of its grain boundaries. All such polycrystalline aggregates are subject to capillary driven competitive grain coarsening phenomena, provided that the temperature is high enough to overcome the activation barriers. In the metallurgical terminology these phenomena are often referred to as grain growth.
It is described as a process by which the mean grain size of an aggregate of crystals increases. The driving force for this results from the decrease in free energy which accompanies reduction in total grain boundary area. As the crystals become gradually larger, the curvature of the boundaries becomes smaller. This results in a tendency for larger grains to grow at the expense of smaller grains.
It should be emphasized that in many engineering applications grain growth is not desirable, as many mechanically beneficial properties of structural metallic alloys such as strength, strain-hardening and ductility are improved inversely to the average grain size.
Given a sufficiently high temperature and no microstructure features which impede grain boundary migration such as second phase particles or impurities that act on the grain boundaries, polycrystals will gradually evolve towards a single crystal. In real microstructures this goal is rarely achieved.
In two dimensions, a metastable state where coarsening ceases, is in principle possible, provided that all grain boundaries have exactly the same grain boundary energy and are connected with each other with 120° angles, so that the microstructure consisting then of equal hexagonally shaped crystals, is free of any grain-boundary curvature so that no capillary driven grain coarsening occurs.
It must be emphasized though that such a microstructure is only mechanically stable (and not in thermodynamic equilibrium) owing to the local mechanical equilibrium of the grain boundaries when abutting under 120°. In two dimensions such intersections are referred to as stable triple junctions.
From a thermodynamic viewpoint the driving force of grain coarsening is the overall reduction in the total grain boundary area per volume. Hence, even a microstructure that is under local mechanical equilibrium is not necessarily also in thermodynamic equilibrium.
In most cases, however, even in quasi two-dimensional situations as sometimes encountered in thin film grain structures, microstructures are not free of local curvatures of the grain boundaries involved. In three dimensions, curvature-free and at the same time compatible grain topologies are not possible in local mechanical equilibrium (MacPherson and Srolovitz, 2007). This means, that in three dimensions all polycrystalline microstructures must necessarily contain some local grain-boundary curvature.
A classical kinetic approach to describe such a gradual coarsening phenomenon can be derived by considering the velocity of a portion of grain boundary which is related to the pressure gradient across it as described above in terms of the phenomenological Turnbull rate expression for grain boundary motion.
This shows us that grains grow at a rate which is essentially proportional to the square root of time when considering that the initial grain size is much larger than the current average grain size .
It has to be emphasized at this point that in this derivation we have only considered the reduction in grain boundary energy for the driving pressure. Possible additional sources that might enter into the total energy reduction are that the grain boundary might be separating grains consisting of different phases, or one grain may have a higher dislocation density than another. If neither of these effects apply, the pressure can arise simply as a product of the curvature of the grain boundary. This pressure acts towards the centre of curvature.
It is interesting to note in that context that it is actually in a real microstructure not the average grain size that acts as a driving force for grain coarsening but it is the local curvature of the interface that matters. In other words, any local grain boundary curvature aims at reducing the total grain boundary area per volume by moving towards the center of the curvature radius. This elementary process of grain coarsening is explicitly guided by the local curvature and the grain does not 'know' if actual grain size.
The actual connection between grain size and local curvature comes simply through the principle that large grains have either very low curvatures, and hence, slow growth rates of their grain boundaries, or the curvature of their grain boundary facets points outwards, towards the surrounding smaller grains.
As mentioned above, a neutral, curvature free microstructure, irrespective of the actual grain size, occurs when all grains in a two-dimensional grain structure have the same hexagonal grain shape and mechanically stable intersecting triple junctions of 120°. A triple junction is the point at which three grain boundaries meet. This local mechanical equilibrium among three intersecting grain boundaries in two dimensions does only apply when all interfaces have the same grain boundary energy.
Mullins pointed out the random grain structures are, however, inherently unstable). In 2D, grain boundaries that are associated with microstructures that are characterized by certain size distribution actually have to ‘curve’ so that they can intersect at triple junctions under preservation of the local mechanical equilibrium. In two dimensions this means that grains with less than six sides have centers of curvature lying inside their boundaries. Conversely, grains with more than six sides have centers of curvature lying outside their boundaries. This gives rise to the driving pleasure derived above, being proportional to both the grain boundary energy and the local grain boundary curvature. The resulting velocity of boundary migration is always directed towards the centers of the interface curvature.
For two dimensional grain structures this observation was cast into the so-called N-6 rule. This rule states that an N-sided grain evolves under 2D grain growth. This so-called Mullins-von Neumann rule shows that actually the number of grain boundary sides determines the crystal growth and not the grain size itself. Topologically it should be noted, though, that larger grains are very unlikely to contain a small number of boundary facets, and so the two formulations are approximately equivalent.
Although in three dimensions this problem is more complicated the main kinetic and energetic characteristics of grain growth remain in principle unchanged, namely, (i) grain boundaries migrate towards their centers of their curvature; (ii) small grains have usually larger curvature values associated with their grain boundary facets and hence reveal higher grain boundary velocities so that these become smaller at the expense of larger grains; (iii) and the average grain size increases with time.
However, one main difference in grain growth exists between two dimensions and three dimensions. In three dimensions no grain topology exists (as in 2D), which satisfies both, zero curvature grain-boundary facets and local mechanical equilibrium at the grain boundary interaction lines and points. The application of 3D surface evolver simulations has recently shown that in average grain shrinkage occurs for grain shapes that have a smaller number of facet areas than 14. Grains that have a larger number of grain facets will in average become larger during grain growth. The minimal-area tessellating grain shape in three dimensions is the 14-sided tetrakaidecahedra, a shape whose surface is made up of six hexagons and eight squares.
I add some reads for more details.
Good Luck!
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Hello. I am currently working on lactide modification, and would like to take this article as reference: A Bifunctional Monomer Derived from Lactide for Toughening Polylactide | Journal of the American Chemical Society (acs.org)
The author uses TLC to monitor the reaction, (Lactide + NBS, substitution of H to Br) but skip the details. I wonder how to visualize lactide/product after TLC since the compound is not UV-active or have any good functional group for dye to react.
Also, I am not sure which solvent should I use for this system.
Appreciate the help!
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Thanks and sorry for the late reply!
I end up just collect/purify samples and use H NMR for that project. I would definitely try your solution for similar projects.
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In some industries, authority provides lunch for their staffs and workers. The kitchen store of these industry regularly receive the supply of frozen chicken meat from meat processing company and they have to make QC report by visual checking. How can they detect bacterial contamination while receiving frozen chicken visualy?
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Detecting bacterial contamination visually on frozen chicken is challenging. Harmful bacteria, such as Salmonella, Campylobacter, and Escherichia coli, are not visible to the naked eye. Bacteria contamination often doesn't show any distinct physical signs, especially in frozen chicken. However, there are some indirect visual cues that can raise concerns: Discoloration: Healthy chicken meat should have a consistent pinkish color. If the meat shows grayish, greenish, or yellowish discoloration, it could be a sign of spoilage, which may be linked to bacterial growth. Ice Crystals or Frost: Excessive frost or large ice crystals on the chicken could indicate that the meat has been thawed and refrozen, a condition that might lead to bacterial growth during the thawing period. Slimy Texture: Although more of a tactile cue, if the chicken appears slimy, it’s often a sign of spoilage or bacterial growth, even though it's frozen. Unusual Odor (upon thawing): While the chicken is frozen, an off odor might not be detectable. However, if it smells rancid, sour, or like ammonia upon thawing, it’s a sign of contamination or spoilage. Damaged Packaging: Tears, holes, or leaks in the packaging can increase the risk of contamination. The packaging should be airtight and intact to prevent exposure to bacteria.
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I want to visualize the bacterial colonies grown inside a closed system made of transparent polymer. For this, I am planning to use inverted fluorescent microscope. Please suggest what magnification will be best 40X or 100X? Also, kindly share the fluorescent dyes to be used to stain bacterial membranes?
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May consider using Permai fluorescence dye.
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So I need to represent a lot of overlapping regions (TFBS) in many promoter sequences (EG at some positions 5 at most might overlap). I have each region start and end points in an excel file with the total length of the sequence.
Doing it by hand is a lot of work. I tried CIIIDER; but I had a problem with the scale bc my promoters are small (<300 bp) and CIIIDER does not allow me to increase the size of the representation as I need it.
Does anyone recommend a software that can do that?
Thank you in advance.
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Hi Sergio,
I recommend Geneious Prime if you need more flexible software than CIIDER since you'll be working with smaller scales of DNA sequences. You can adjust and customise the settings to visualise the overlapping regions easily. Of course, other options like Snapgene, IGV, and ApE exist, but I'm not entirely familiar with those. All the best; I hope it helps!
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Dear friends, I need to transform my qPCR data into a heatmap to make it more visualized. I have seen this form in several articles, but I'm not sure about some details:
1. Should the P value be presented in the heatmap? If so, how to do that?
2. Is there any requirements of sample size to make a heatmap?
Thank you for any advice!
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1. No. The purpose of the heatmap is to visualize/explore patterns across different genes and conditions, not to present result of hypothesis tests.
2. No. But the larger the sample size, the easier it will be to distinguish random noise from robust patterns.
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How is it possible to visualize nanoparticles (3-7 nm) dispersion in liquid crystal? Is it possible by Scanning Electron Microscope?
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Natalia Kasian Brownian motion will mean that the particles will be moving violently in a liquid. However in a nematic LC, they could be more constrained to a plane, but I have no idea. Also these sizes are close to the resolution of SEM. I’d look to freeze-fracture or, better still, cryoEM for morphological studies of your system.
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And what are the potential applications of these technologies in areas such as smart agriculture or autonomous exploration?”
Pathfinding refers to the process by which a robot navigates from one point to another. This process involves the use of sensors, software, and sometimes visual cues.
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- Programmable lights can be used as a tool to guide robots in different environments. These lights range from software-controlled LEDs to more complex systems that display specific patterns or visual information to the robot.
- The accuracy of robots can be improved by incorporating smart lighting systems that provide clear signals or instructions. For example, lights can be used to mark paths or to alert the robot when it is approaching obstacles.
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Hi,
I'm currently working on a project where I need to plot the atom-projected band structure using GPAW.
I've been able to calculate the band structure for my material, but I'm having trouble figuring out how to separate and visualize the contributions from different atomic species.
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Dear Jhon W. González simple question, Do you think, we're made of mechanics atoms? I do not think I'm!
Did you ever read fine print of CERN or any others?
CERN is following standard modeling of atom. Yet after seven decades everything is in stage of theory of outcome. Nothing is for definite.
Up this date nothing inside of atom ( proton/neutron, electron/positron, quarks, Higgs) never been observed or proves of mechanical atoms phenomenon.
Sadly, our science connecting mechanical physics to nature that it is not working mechanically, unfortunately some one like you accepting one-dimension static mathematic for three-dimension of nature that it is changing constantly.
At end of day, I don't think you know anything about atom or nature of our universe.
prove it, you know!
Reading of last century ago, and accepting modeling, prediction, postulation is not science, yet you follow.
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Hi all,
My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets?
The idea would be to activate platelets with agonists and potentially be able to see the formation of thrombi.
Does anyone know if this is possible? Any SOPs, tips and tricks?
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Thank you Fahad Alshehri
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I used a Li-COR Flux system to measure respiration in different soil types. The question is, my data is mostly positive values but on a small scale (0 < x < 1). However, there are a couple of days where I got negative values.. VERY negative (-43, -32...). They are not outliers since I did triplicates per day.
I don't want to delete this data, I think maybe something was happening in the microbial communities those days. But the difference in scale doesn't allow me to visualize the data in scatter plots.
I was thinking about some type of standardization? But I don't want to alter the dC/dt values.
Thank you!
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As per the LI-COR instruction manual for Li-8100A, negative flux values are valid measurements. You should not discard it from your analysis.
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In basin delineation and hydrological modeling, selecting the exact value in Flow Accumulation to identify streams is a critical step. The Flow Accumulation tool calculates the accumulated flow as it traverses down a landscape, which can be visualized as the number of cells contributing to flow into each cell. This accumulated flow can then be used to define stream networks by setting a threshold value.
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Good morning,
to determine the threshold value I know of several ways. The first is to determine a reference stream, with a known source. This can be done by taking a field measurement or visually interperpetrating a shaded relief. Then read the Flow Accumulation value in the neighbourhood of the designated reference source. The second method is the trial-and-error method, but to avoid wasting too much time I recommend automation in the R programming language. A script automatically tests different threshold values and generates vector files, at the end you should choose the one that is closest to reality. In general, it is best to go into the field, but when this is not possible, consider drone inspections, reviews of data bases such as Opeen Street Map or local information sources, atlases and cartographic studies.
If you have any questions, please feel free to contact me, I can help as an R package developer.
Best regards,
Jakub
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Dear community,
I want to perform an analysis of the risilin localization within the legs of myriapods and would like to visualize the localization of the resilin components in different taxa.
Do you have any recommandations for a commercial antibody (doesn't matter if its mono- or polyclonal))
Thank you very much.
Best,
Benjamin
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Not a recommendation, but Biorbyt does carry a Rabbit pAb against Drosophila resilin: https://www.biorbyt.com/resilin-antibody-orb807146.html
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CONTEXT: Achieving the 2030 UN agenda for SDGs requires integrated, citizen-centric approaches and holistic interventions for delivering transformative results on the social, economic, and environmental dimensions. Current initiatives in many emerging markets are slow and face adoption and scalability challenges at a local and systemic level due to lack of in-depth understanding and prioritization of complex issues, many of which relate to each other, like the SDGs. A good starting point is to take a human-centric approach starting with developing deeper empathy with citizens to visualize and design a future for the citizens of the country. But who better to share authentic insights and see a better future than those who will live it – CHILDREN.
Within many cultural contexts it is recognized that drawing techniques can provide a relatively easy way to gather personal and socio-cultural information, both from and about children, as well as offer valuable insights into children’s experiences, ideas, feelings and environmental perceptions. Childhood and children are now seen as worthy of investigation in their own right. Much recent studies has emphasized the importance of listening to children’s perspectives on issues that are important and relevant for them. The advantage of using drawing is that this is self-reported data.
These drawings can be used to explore the world they live in, and therefore understand the social, economic and environmental issues at the local level. Art activities provide a psychologically safe and creative way for children to express their strongest desires in a visual form without relying on words or the need to know a language for expression.
ASK: I am looking to conduct a literature review on visualization, image interpretation and content analysis techniques for issue identification in the drawings and artworks of children. In addition, I am, therefore, seeking projects are worthy of mention based on their quality of work and potential to scale in the aforementioned areas. A good example is Room 13 that started in Scotland and Project Dream On India - that captured 10,000+ artworks of children from pan India including Jammu and Kashmir.
Would appreciate your references, thoughts, ideas et al.
Thanking you in anticipation.
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Sharing the new TEDx talk on project Dream On India -
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I made a material which loos like liquid,
but when I do the rheology test,
the G'>G" .
In my opinion, I think it means it was a solid or a gel.
The test parameters are like this
and the result is figure 2
Would anyone be able to advise why this may be? I'm not really sure how to further optimize my parameters as I've tried several different ones already.
Thanks!
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This may be due to strong interactional forces between the components of material. This also shows that the material is highly viscous and May be in gel form.
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Hi
I am new to 3D culture, live cell imaging and confocal microscopy. Is it possible to visualize epithelial and endothelial cells by live cell imaging for a period of time using confocal? In addition, I would like to visualize the bacterial cells and the biofilm produced on the 3D culture over a course of time?
I do have the information on the antibodies to be used for labelling to visualize epithelial & endothelial cells, and biofilm. However, the protocol needs the cells to be fixed. Also, I could get information on the nucleic acid stains which can be used on dead cells to visualize bacteria and eukaryotic cells.
Any thoughts on this?
Thanks in advance
Warm regards
Bindu
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Thank you Dr. Woo.
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i seeded CACO2 on 0,4 um PET transwell insert, i want to see my cell layer in transversal view in order to monitor the polarization and formation of brush border. To do this, i fixed with 4% PFA in PBS for 30 minutes, then put in alchol 70% until the processing.
I continued the dehydratation with 95% alchol for 1h (twice), 100% alchol for 1h (three times), xylene for 1h (twice), paraffine overnight, and one change of paraffine the day after for one hour and then embedded in paraffine.
The problem apperead during the cutting, because the insert started to curling up and seems that the sample is detatched from the surrounding paraffine.
How to solve this problem? What can you suggest me to change in the protocol?
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I am trying the same unsucessfully. Follow
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Understanding a student's learning style is crucial for effective education. The VARK model, one of the most common frameworks, categorizes learning styles into four types: visual, auditory, reading/writing, and kinesthetic. Each style has distinct characteristics; for example, visual learners benefit from diagrams and charts, while auditory learners excel with spoken information. Recognizing these preferences allows educators to tailor their teaching methods, creating a more inclusive and productive learning environment for students.
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I think this is not possible always. However, teachers can follow mixed learning style to reach more students.
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example as picture
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Uma lacuna é uma falta em uma superfície pintada. Pode ocorrer por diversos motivos que levaram à perda de uma ou mais partes de uma pintura: ressecamento do ambiente, quedas ou má intervenção
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My concern is to collect information for my academic research in photojournalism and visual journalism.
What are the Theories, Models and Methods to analyze, interpret, decode, evaluate and explain visual language in academic research concerning Photojournalism and Visual Journalism?
is there any helping material available?
Thanks in Advance
Rizwan Ali
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Basically the same as those used to examine any artwork: formalistic, iconographic and iconological analyses (for the last two, see Erwin Panofsky, Studies in Iconology, 1st published in 1939, and constantly reissued). But you might also want to study the way that Marshall McLuhan does so in his book, The Mechanical Bride, published in 1951, which specifically refers to newspaper texts, pictures and ads. Both these books lay the groundwork for much of Post-Modernist art analysis.
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I have atomic coordinates and the structure is cubic cell with lattice parameter 10.001 Angestrum. How could I prepare the cell vectors to visualize in Vesta? Thanks.
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Kübik merkezli olduğu için bir tanesi merkez atom diğerleri onun etrafında kübik şekilde dizilir. (10 küpü +1).örgü atom modeli şeklinde dizilerek sürekli kübik yapı oluşturur. En son atom merkezi atom seçilebilir, bağımsız ve serbest hareket edebilir.
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I have data consisting of two trends over time.
For example, the GDP and net immigration figures of the UK, from 1950-2020 (not the real data).
I can plot the 2 trend lines on the same graph and visually examine if they look like they correlate over time. However, is there any way to examine if they statistically correlate over time?
Note, this need to be a simple analysis that one can do in MS excel of other free software (e.g. JASP), as it is for a student.
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Yes, the same meaning: "rolling the window" = like = sliding the window.
Good luck Joseph Whittaker.
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Would it be possible to visualize vortices with lengths on the Kolmogorov scale using visualization techniques such as Schlieren or Shadowgraphy?
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Hello Junhao Rong, I've given some serious thought to a schlieren setup closer to that of a microscope. However, a typical microscopy lens, such as the ones used to measure droplet sizes in sprays, is not capable of producing schlieren images. With such a lens, we can only obtain shadow images, which I believe lack the sensitivity required to visualize vortices. I was considering that a potential solution might be to use a lens with a long focal length on the camera. Thank you for the discussion.
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I would like to create visually appealing diagrams for my publications. For instance, to create graphical abstracts for articles, I want to know which computer tool can assist me in this regard.
Thank you in advance for your responses.
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Personally I used Biorender recently. It is a rather user-friendly tool (with limitations). You can try for free if it suits you. The only real drawback, it is not for free if you need publication quality pictures.
Best regards.
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I found to use a wrong secondary Ab for my WB when I visualized it with ECL, can I wash with TBST for several times and incubate the right secondary Ab? Another question is why my bands are not flat? What is wrong with my electrophoresis? Thanks!
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Thank you very much for the suggestions, I will try again. Best wishes. Malcolm Nobre Stefanie Meyer
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I need to visualize specific regions of mouse brain (e.g., substantia nigra). However, cryosectioning the entire brain tissue is resource intensive and not feasible. Is there a relatively quick and easy way to identify the anatomical location of previously cut brain sections without the use of staining or immunofluoresence?
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Yes there are alternative ways to roughly identify anatomical locations without additional staining.
Here are two approaches that help you
1: Learn to identify key anatomical landmarks in the mouse brain. The brain atlases can be helpful resources. By comparing your sections to the atlas, you can get a rough idea of the anatomical location.
2: Another way is to use a bright-field microscope with a low magnification objective to get an overview of the entire brain slice. This may help you identify gross anatomical features.
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PARADOX
This PowerPoint begins with the Ambiguity Paradox: Everything is ambiguous; however, nothing is ambiguous.” Perhaps all words and sentences are ambiguous, if they are not seen or heard in the larger context. However, the larger context (both linguistic and non-linguistic) resolves almost all of the ambiguities--Except when the speaker is intentionally trying to be ambiguous, as with linguists and politicians.
Then we go on to discuss science fiction and the “grandfather paradox,” and the building which is larger on the inside than the outside, and some “Catch-22 paradoxes,” some visual paradox, and many other paradoxes from the 16th, 17th, 18th, 19th, 20th, and 21st centuries as expressed by Montaigne, Beaumarchais, Josh Billings, Henry Wheeler Shaw, Oscar Wilde, Marshall McLuhan, Joseph Heller, Gilbert and Sullivan, and others.
Gilbert and Sullivan often relied on paradox for comic effect. In The Pirates of Penzance, they composed a song about paradoxes:
How quaint the ways of paradox!
At common sense she gaily mocks!
A paradox, a paradox,
A most ingenious paradox!
Ha! Ha! Ha! Ha! Ha! Ha! Ha!
Ho! Ho! Ho! Ho! Ho! Ho!
Poor Fredrick was to be the apprentice on a pilate ship until he was 21. But, by mistake, he became the apprentice on a pirate ship until he was 21.
But he was born on February 29th in a leap year, so he was only five birthdays old. For some ridiculous reason, to which, however, I’ve no desire to be disloyal,
Some person in authority, I don’t know who, very likely the Astronomer Royal, Has decided that, although for such a beastly month as February, Twenty-eight days as a rule are plenty, One year in every four his days shall be reckoned as nine and twenty.
Through some singular coincidence—I shouldn’t be surprised if it were owing to the agency of an ill-natured fairy—You are the victim of this clumsy arrangement, having been born in leap-year, On the twenty-ninth of February.’ And so, by a simple arithmetical process, you’ll easily discover, That though you’ve lived twenty-one years, yet, if we go by birthdays, You’re only five and a little bit over!
Ha! Ha! Ha! Ha! Ha! Ha! Ha!
Ho! Ho! Ho! Ho! Ho! Ho!
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Don L. F. Nilsen --- Don, It is true if you also assume there are distinct differences among our ideas. The other possibility "its all the same". In a way we form separations to study paradoxes. But without our desire to see separate parts the distinct differences disappear and we "see" the whole. - And, paradox (one of the academic "games" we love to play) is not apparent.
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Not able to view the downloaded model from ClusPro using PDBSum. Since both the docked proteins contain single A chain, PDBSum shows it as single image. Please give suggestions. Also tried to save the chains as separate using PyMol, still didn't work.
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I think with the help of LigPlot+, you can see the 2D protein-protein interaction.
But in any case, you have to change the chain identifier of one of the proteins. you can manually edit the chain identifier directly in the PDB file using a text editor. Locate the lines that define the chain identifier and change the chain identifier accordingly. Save the modified PDB file after making the changes.
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I'm a secondary school teacher who is starting a new research in the field of Visual Thinking Strategies (VTS) in Ibi, Spain.
We are a group of 4 teachers that have been working VTS in our school with students between 12 and 14 years old during the last 5 years.
This year we have started a new research related to a two stages VTS activity. The first stage is carried out by one group of students and consists of describing a image. The second stage is developed by a different group of students and consists of drawing a picture after having listened the description done by the first group.
The results of this research can be applied to teaching people with a disability (blind people).
Do any of you have been working in this field?.
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Yes even i m doing research in same field.I m doing my research on junior college students who are describing diagram looking at their characteristics features.
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ALTERNATE VIEW: what is big-data-driven imagery besides something threatening? How can we harness this new tool for unselfish projects to help all of us and the planet?
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Dear Colleague Daniel Martinez,
Thanks! Your insights from the fields of ecology and our global work on natural resource conservation are very helpful for me to contextualize how this new tool of analysis can function responsibly. Thank you for taking the time to reply.
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I have conducted a detached leaflet inoculation assay to measure/compare disease severity. I used image analysis software to get the % diseased area. While using these actual numbers is the usual practice, is it acceptable to convert those percentages into a scale, such as 0-15, or 1-12 like the H-B scale used for visual estimation?
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s. Jacob Schneider I assume that this question is related to the recent one (Do you recommend using a Disease Severity Index if you have digitally measured diseased area?). Transformation of leaf area percentages into a scale is possible, but you will lose a significant information content.
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Hello ResearchGate Members,
I hope this message finds you well. I am currently exploring different tools for visualizing frequency collocations extracted from the AntConc program using network graphs. While I have tried VOSviewer and KH Coder, I've encountered challenges as they don't seem to generate graphs based on simple frequency.
I would greatly appreciate any recommendations or insights from the community on alternative tools or methods that effectively visualize frequency-based collocations through network graphs. Your expertise and suggestions will be invaluable in enhancing my research visualization.
Thank you in advance for your assistance, and I look forward to learning from your experiences.
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Thank you very much, Illia, for sending me the figure. I guess the frequency of the collocate is given via the saturation of the colour of the spot, whereas the distance from the node indicates the real distance of the collocate from the node in a text (your span is -5/+5).
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Hi.
I want to perform a docking on a double-helix DNA, about 20 bp long.
I am using the free version of Discovery Studio Visualizer.
I want to energy-minimize my DNA before the docking, but DS has many minimization options and I'm not sure which is the most appropriate to choose.
Can anyone help?
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May I know if yu managed to do energy minimisation for DNA?
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To be developed.
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Here are a few perspectives to consider:
- As AI systems become more capable of generating creative works like art, music, and writing, the notion of originality and authorship gets more complex. An AI system is trained on vast datasets and learns patterns and styles that it can remix into new creations. So even its most imaginative outputs are derived from human-created works. This leads some to question whether AI creations can ever be considered truly original.
- However, even for humans, complete originality is arguably rare - we are all influenced by and build upon the ideas, styles, and techniques of those before us. The creative process often involves remixing, iteratively improving, and finding new combinations, even when generating highly novel work. In this view, AI expands the creative palette, though attribution and intellectual property issues remain open questions.
- Visual collages assembled from multiple sources also have a long history of artistic use for creative expression and social commentary. The juxtaposition and recontextualization of disparate elements allow new insights and meanings to emerge from the composite. This may allegorically relate to emerging AI capabilities - synthetic media that remixed elements into a new "subject authorship" defined by the arrangement itself more than the original components.
- Some believe synthesizing creative works from abundantly available digital data simply accelerates the postmodern deconstruction of originality and sole authorship. It further dispels the notion of art and media requiring a human creator in an fixed historical context. From this perspective, evaluating AI outputs on originality alone misses their potential - the value may lie more in what effects they catalyze or perspectives they introduce.
There are good arguments on multiple sides, but the questions raised seem increasingly important as interactive AI becomes more ubiquitous. As creators, interpreters and consumers of synthetic media, an open and thoughtful discourse seems needed to guide both technology and policy. But I have only touched the surface of these complex issues from a non-expert view. There are likely many deeper dimensions left to consider for those immersed in visual arts and media studies. Please let me know if you would like me to expand or clarify any part of this response!
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Can artificial intelligence read minds? If so, how does that impact automated language translation? My answer: Artificial intelligence either can already read minds or potentially probably will be able to. Assuming the information in thoughts is translatable regardless of whatever language the person thinks in, the translation between humans may come faster. Humans are very visual creatures and language is fluid(always representing imagery in thoughts including extreme abstractions) thus, artificial intelligence translating one language to another could end the need for human language altogether, or maybe at least extremely facilitate translations.
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Artificial Intelligence (AI) has made remarkable advancements in recent years, but the idea of it being able to read minds seems far-fetched. However, if AI were capable of such a feat, it would undoubtedly revolutionize automated language translation. The impact would be immense and could potentially bridge the gap between different cultures and languages.
If AI could read minds, it would have a profound effect on automated language translation. Currently, translation algorithms rely on input text to generate accurate translations. However, this method often falls short in capturing the true meaning and nuances of a particular language. By being able to read minds, AI could understand the intent behind the words and phrases used by individuals.
This newfound ability would significantly enhance automated language translation by providing more context and accuracy. It would enable AI systems to comprehend idiomatic expressions, cultural references, and even sarcasm – elements that are often lost in current translation methods. As a result, communication between people speaking different languages would become more effective and seamless.
Moreover, if AI could read minds accurately, it could eliminate the need for any intermediary step in communication. People wouldn't have to type or speak their thoughts; instead, they could simply think them while AI translates them instantaneously. This direct mind-to-mind communication would break down barriers like never before and foster understanding among diverse groups of people.
However, despite these potential benefits, there are ethical concerns surrounding mind-reading technology that must be addressed. Privacy is one major issue that arises when discussing AI's ability to read minds. If an individual's thoughts can be accessed without consent or control over who can access them, it raises serious questions about personal autonomy and privacy rights.
In conclusion, while the idea of artificial intelligence reading minds may seem like science fiction at present time; if achieved successfully in the future - it will undoubtedly have a significant impact on automated language translation. The potential benefits include improved accuracy in translations as well as direct mind-to-mind communication. However, ethical considerations must be carefully addressed to ensure privacy and consent are respected.
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TEM MODES
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Thank you sir
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I'm trying to set a new subject
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By utilizing SAR satellite products, one can assess the state of the ground surface prior to and following the eruption. Please refer to the following article for an illustrative example.
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I am docking small peptides up to 6 AA chain long. After so many month of trying to find a successful peptide-protein docker, i finally found Swisssdock but now the challenge is how to analyse the results. I cannot open the results in ChimeraX and pyMol which are so much farmiliar with many of us. USCF chimera I can open the results but it is difficult to create images for publications. Can I please get help with a step by step guide how to view the results from Swissdock using Discovery Studio Visualizer to analyse the interactions .
I will be greatful and highly appreate any help rendered.
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Here's a general process to do this:
Run SwissDock: Start by running your docking simulations in SwissDock to predict the binding of ligands to a protein target.
Export the Results: After the SwissDock simulations are completed, you can typically export the results in a format that is compatible with molecular visualization software like Discovery Studio Visualizer. SwissDock may provide options to export the results in common file formats, such as PDB (Protein Data Bank) or MOL2 (Molecular 3D Structure) files.
Open in Discovery Studio Visualizer: Launch Discovery Studio Visualizer on your computer. Use the "Open" or "Import" feature in Discovery Studio Visualizer to load the exported SwissDock results. This usually involves selecting the PDB or MOL2 files containing the protein-ligand complex structures.
Analyze Ligand Interactions: Once you have imported the SwissDock results into Discovery Studio Visualizer, you can analyze the ligand interactions with the protein target. You can visualize the binding modes, hydrogen bonds, hydrophobic interactions, and other molecular interactions between the ligands and the protein.
Visualize and Interpret: Use the visualization and analysis tools in Discovery Studio Visualizer to examine the docking results, study the ligand-protein interactions, and gain insights into the binding affinity and stability of the complexes.
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Is it necessary to have a special microscope filter to visualize transient transferred mKeima cells? The microscope I have been using has the typical GFP and mCherry filters, but not a special filter to see mKeima fluorescence.
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Dear Emily Fr ,
You will know, that mKeima is a fluorescent Protein to measure the intracellular pH. As you can find here:
Excitation should be around 440 nm and you will have to measure two emission wavelengths i.e. 650 nm and 600 nm, but I have never used mKeima. Standard GFP and mCherry filters will not work, since a GFP filter might be able to excite your protein the band pass filter for the emission will not match the emission of the mKeima. And mCherry Filters will not excite your protein although they should have the right emission filter. If you have the possibility to use a long pass filter for the emission you might be able to check the transmission. Or you can try to use the emission filter of the mCherry Filter set in the GFP Filter set instead of the existing GFP Emission Filter, but that might depend on the dichroic filter.
Best wishes
Soenke
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I have some single channel EEG data which are in the .csv format. How to import this .csv File to EEGlab and analyze them? Or is there any way to convert these data to .edf or .bdf format (Biosemi Data Format)?
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run EEGLAB
in command line write:
M = csvread("path/file.csv")
then import the "M" array using the menu "File > Import data > From ASCII/float file or Matlab array"
Don't forget to enter the sampling rate and specify 'M' as a variable to import.
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What is visual fatigue?
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It is something caused by intense eye use. Its main cause is looking at computer, cell phones for prolong period of time.
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With the increase of office hours, the human eye will produce visual fatigue, but with the increase of time and gradually deepen the visual fatigue, how is the change process?Working on different media, the process of eye fatigue change with time is different. What is the difference?
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As any weariness, degree of visual fatigue depends on spectra of eyes loads (stress). So, for person working in different media the dynamicis of this process with time is changing .
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In the realm of data visualization in Python, which library stands out as the most versatile and effective tool, accommodating diverse data types and producing impactful visual representations?
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I believe it's more about how you use the tool rather than which tool. But Matplotlib can do quite well for many common requests. They have a library of examples where you can learn how to create more complex plots: https://matplotlib.org/stable/gallery/index.
There's seaborn (developed based on Matplotlib) as well, if you want something to be done real quick, try that. I'd suggest you start with Matplotlib first, understand their principles, and then move on to seaborn, where customization requires you to add more Matplotlib parameters. See the gallery here: https://seaborn.pydata.org/examples/index.html.
There's also plotly (https://plotly.com/examples/), suitable for developing interactive apps. It looks really potential, but is an independent platform from Matplotlib, so will take you a while to learn.
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Hi everyone.
we are doing IF for checking the translocation of a protein between cytoplasm and nucleus in SY5 cells , and I need a marker better than NeuN to visualize the whole cell and make it easy to distinguish between cytoplam and nuclei.
Does anyone have any suggestions for a marker instead of NeuN for SH-SY5Y cells?
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I really appreciate your help , thanks :)
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Is it possible that the amplification failure products can be visualized in electrophoresis? Due to the failed amplification results it shows bands in my electrophoresis with bands that are quite clear. My amplification curve clearly shows amplification failure, but when I look back at it with electrophoresis there are some obvious bands, how is that possible?
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I think this band is because of the primer bind with itself we call it dimer
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Automotive manufacturers are increasingly integrating augmented reality (AR) and virtual reality (VR) technologies to revolutionize various aspects of vehicle design, prototyping, manufacturing processes, and customer experiences. These immersive technologies offer significant benefits in the era of advanced automotive engineering and smart mobility solutions. Here's how AR and VR are being utilized in the automotive industry:
  1. Vehicle Design and Styling: Designers and engineers use VR to visualize and interact with 3D models of vehicles, enabling them to explore different design iterations and assess aesthetics, ergonomics, and functionality. VR design reviews facilitate efficient collaboration and decision-making among cross-functional teams.
  2. Virtual Prototyping and Simulation: VR allows automotive manufacturers to create virtual prototypes of vehicles and conduct realistic simulations of various scenarios, such as crash testing, aerodynamics, and thermal analysis. This streamlines the development process, reduces physical prototyping costs, and enhances safety assessments.
  3. Manufacturing and Assembly Processes: AR is applied on the factory floor to guide assembly line workers with real-time instructions and visual overlays. AR-assisted assembly and maintenance improves productivity, reduces errors, and enhances worker training and skill development.
  4. Quality Control and Inspection: AR and VR enable technicians to perform detailed quality control inspections using digital overlays, highlighting potential defects or deviations during manufacturing processes. This enhances product quality and reduces defects.
  5. Customer Experience and Marketing: Automotive manufacturers leverage AR and VR in showrooms and marketing campaigns to offer immersive and interactive experiences to customers. VR-based test drives and AR-enabled product presentations allow customers to explore vehicle features and configurations.
  6. Virtual Showrooms and Configurators: AR and VR technologies power virtual showrooms and vehicle configurators, enabling customers to customize vehicles, explore different options, and visualize the final product before purchase.
  7. Service and Maintenance: AR is utilized to provide service technicians with real-time diagnostic information, step-by-step repair instructions, and overlay information on the vehicle, simplifying maintenance tasks and reducing service time.
  8. Training and Skill Development: AR and VR-based training programs are used to educate service technicians, assembly line workers, and sales personnel. These interactive training modules enhance skills and knowledge retention.
  9. Design Validation and Customer Feedback: VR allows automotive manufacturers to conduct virtual focus groups and user studies to gather customer feedback on vehicle designs, features, and usability.
  10. Autonomous Vehicle Development: AR and VR technologies are utilized to simulate real-world driving scenarios for testing and validation of autonomous vehicle systems, reducing the reliance on costly physical road testing.
The integration of AR and VR technologies in the automotive industry is transforming the entire product lifecycle, from design and manufacturing to sales and customer support. These immersive technologies not only improve efficiency and reduce costs but also enhance the overall customer experience and drive innovation in advanced automotive engineering and smart mobility solutions.
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I don't have first hand information, but I would think VR will be a good way to give people an experience of driving the vehicle before buying the car.
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I am doing research on ERP signals using visual stimulus. I am using BioRadio device SDK to get the ERP signals. I can obtain the time and values of the electrodes being used.
While going through ERPLAB tutorial, I see that while given an EEG.set file, it generates EVENTLIST using a set of 'event codes'. Would someone explain to me how to obtain such event codes ? Are they responses to go/no-go task during any visual stimulus ? Do I need to set flag as event codes for such responses to tasks ?
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Hi,
Event codes in ERP research denote significant experiment events. They're sent from the experiment software to the EEG device and analysed using tools like ERPLAB.
Hope this helps.
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I am working with an optic nerve crush model and a neuroprotective/regenerative treatment. Among the planned assessments, visual acuity tests are intended to be conducted. However, optic nerve crush is only performed on one eye, while the other eye remains intact (for bioethical reasons and to prevent the rat from becoming nearly blind). In the visual acuity test, we obviously want to evaluate only the damaged eye and determine if the treatment improves visual capacity. However, we are unsure how to close the intact eye in a way that prevents the animals from having visual input from that healthy eye, without distracting them or causing them to touch their eye, so that their attention remains focused on the visual task. I would appreciate any comments or experiences from anyone who has conducted visual tests in this single-eye model. Thank you.
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Welcome
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1. for visual observation of LAMP with UV Light, can we use geldoc or is there any other tool that can be used to view the LAMP results?
2. How to dilute the LAMP primer from 100mM concentration to 5mM.
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I have got two questions and before asking them I would just like to tell you the ways and cues that the human brain uses to make up the perception of time.
1. We use images and our brain actually in some part holds these images, of the past. We differentiate between the past and the present at least through visual stimulus by referring to these images and the difference between past and the present.
2. This includes all sorts of perceivable change.
Now I would like you to visualize a space where every observable actually attains a constant value and the images in your memory, that aid conceiving the notion of time, are of this one state.
Note* Even the state of the observer's body is constant. There's no change in any possible stimulus.
Question: Will time still exist?
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A good question. The answer is not that simple. But I will try. Please look at the videoclip (at 589 sec.) in which all functional perception processes within the grasping of a coffee cup is explained. Do you understand the commonalities between the alarm clock and the coffee cup?
Please let me know and then we discuss this further.
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I was doing some research, and I have read somewhere on the internet that is not recommended to use homogeneous range of values for color progression between classes (eg., 20%, 40% 60% 80%, 100%) when we are using "value" as visual variable in cartography.
Than, I assumed there might be some "rules" to define a good color progression!
For example: "Black 0, Black 10, Black 30, Black 60 and Black 100" which corresponds to a variation of 10% between the second and first class, 20% between the third and second class, 30% between the fourth and third class and 40% between the fifth and fourth class.
I would like to kindly receive your opinion/feedback about this topic.
Thank you in advanced..
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Dear Patrik Silva, it depends mostly on your specofic task and your aesthetic sensitivity. Feel free to use your own color scheme that most suitable to your goal. For example, use contrast colours if you need to unveil some thresholds, etc.
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Hello
I need to convert ligand-receptor interactions from 3D to 2D. But my Discovery Studio Visualizer software does not have this capability. How can I access the download link of version 4.5?
When I enter the relevant site, I encounter errors to fill in the registration fields.
Thanks in advance for all the help
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Lida Ghaffari To access version 4.5 of the Discovery Studio Visualizer software, you can follow these steps:
1. Visit the official website of Discovery Studio Visualizer or the software's vendor website.
2. Look for the "Downloads" or "Product Downloads" section on the website.
3. Find the version 4.5 of the software in the available downloads. It may be listed under previous versions or archives.
4. Click on the download link for version 4.5. If the link requires registration or login, proceed to the next step.
5. Fill in the registration fields with the required information. Ensure that you provide accurate details and complete the registration process.
6. After completing the registration, you should receive access credentials or a confirmation email.
7. Log in to the website using your newly created account or the provided credentials.
8. Locate the download link for version 4.5 again and click on it to initiate the download.
9. Follow the installation instructions provided with the software package to install Discovery Studio Visualizer version 4.5 on your system.
If you encounter errors or difficulties while filling in the registration fields, ensure that you have entered the correct information. Double-check the required fields and make sure to provide all the necessary details. If the issue persists, consider reaching out to the software vendor's customer support for further assistance. They should be able to provide guidance on accessing the specific version you need and help resolve any registration-related problems.
Please note that software availability and access procedures may vary over time, so it is advisable to visit the official website or contact the software vendor for the most up-to-date information regarding the download and installation of version 4.5 of Discovery Studio Visualizer.
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I am trying to model log reaction times (logRT) as a function of movement direction (categorical with two levels: 'right' and 'left') and reward (categorical with two levels: 'reward' and 'control'). As fixed effects, I enter direction and reward (with interaction term) into the model. As random effects, I have random intercepts and slopes by session number for the effects of both direction and reward. Additionally, given that the variance across levels of reward is clearly different, I allow the model to assume different variances for different levels of reward. Visual inspection of residuals plotted against predicted logRT as well as residuals plotted against predictors (see the figure below) reveals an obvious deviations from homoscedasticity.
var_reward <- var(df$log[df$reward == 'reward'])
LMM1 <- lme(logRT ~ direction*reward,
random = ~ reward + direction | session_no,
data = df,
method = "ML",
weights=varIdent(c(reward = var_reward), form=~ 1 | reward))
My questions are:
1. Do I need to quantify the deviation from homoscedasticity by running tests like Breusch-Pagan to prove that the residuals are heteroscedastic? Or visual inspection of such plots is a proof of heteroscedasticity?
2. Are you aware of any R packages that serve the purpose of functions below for linear mixed models?
- bptest{lmtest}
- white-test{whitestrap}
- bgtest{lmtest}
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Masih -
I think what you really want to know is "how much" heteroscedasticity you have. A "test" that says you do or don't have it would be triggered by what effect level? and Impacted by sample size how? What would you do if you 'found' heteroscedasticity?
When you use a log transformation you complicate interpretation. And your model is more complex than what I want to show you. But what is below may give you some ideas:
In a regression, we can expect heteroscedasticity where variance increases with increased predicted-yi (the size measure). Modeling the heteroscedasticity in a weighted least squares regression can work very well. You might consider the following:
and
Knaub, J.R., Jr.(2021), "When Would Heteroscedasticity in Regression Occur?"  Pak. J. Statist., Vol. 37(4), 315-367, https://www.pakjs.com/
Best wishes - Jim
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Information-light-energy enters an interior space of its own volition, bringing with it, pertinent visual information about the nearby environment external to that interior space as proven by the Camera Obscura.
However, this is deemed not interactive and that no intelligence entity is involved in that process.
If that is the case, then that is the case for all visual media surely, because light-is-visual media.
Interested in any thoughts on this.
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Of course, you must be winding me up, yah? :-)
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Hi everyone,
for a paper I am preparing a plot that links source, method of preparation, and application in a circle-like structure. To discuss it here, I prepared a sketch that should be easier to follow without prior knowledge of my specific field of research (see attached Graphic).
The three circle segments represent vegetables, things you can make from them, and places, where this food might be offered. Lines then connect Veggies, preparations, and types of restaurant, that typically go together.
Examples: Sweet potato chips and potato chips are a thing, hence a connecting line (Zucchini chips and pumkin chips not so much). Vodka is made from potatos, but not sweet potatoes, pumpkin, or Zucchini, and it is served in Pubs, and maybe fine dining restaurants, but rather not in Italian restaurants or Fast-food parlors.
Things I like about this plot-type are:
-I find it aesthetically pleasing.
-You can pretty easily identify niche and common entities from the number of lines originating there (like potatoes and soup going with kinda everything, and Zucchini and Vodka being rather picky).
-Following the lines from one entity, you get to all associated entities, making it quite easy to reference neighbors in the network.
-Unlike in a network graph, entities are not spread about the whole network, but neatly organized on the outside for reference and added text.
-Entities are easy to group into categories. More or less categories, than the three in the example would be possible.
In the example, the categories are all underlaid by a primary color (yellow, blue, red) and each line has the secondary color (orange, green, purple) of the categories it connects, to make it easier to follow the lines. However, the information density could be increased further by formatting the color and strength / thickness of lines. For example, you could code a probability or strength of association by a line color or by line thickness, like: How probable is it to find vodka at a fine dining restaurant; coded as color of the connecting line on a red-blue gradient like in a heat-map, or by line strength.
My questions are:
-Is this plot a new thing, or is there a plot type, that's basically the same, that I just don't know about.
-Does it make sense to display information in this way, or is there a more approachable or more aesthetically pleasing way of conferring the same information?
-What to call this kind of plot? My initial thought was cobweb plot, but that's already a thing. I then thought dreamcatcher, doily, or weaving-frame diagram, which don't seem to be taken already. The design is visually inspired by a circos plot, but otherwise doesn't share a lot of similarities. I am hence a bit hesitant, to call it "narrative circos plot" or "Network circos plot".
If you want to use this kind of plot, I'd be super happy to see your work! Would be cool, if you'd reference or link this post.
Also shout-out to Biorender, where I threw this plot together. However, I am sure, you can also make a plot like this with less manual moving of lines and icons, if you know your R (or GIMP or Inkscape for that matter).
PS: Please spare me your comments, that it actually is possible to make Vodka from Zucchini ;-D it's about the graph style, not the specifics for this one. The example graph is not super polished, but you get the picture. Also I think it's clear, that I am not researching the culinary scene, but I found that an easy and accessible example.
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So far I found out that this would probably be seen as a subtype of Chord diagrams. So an individual name might not be warranted. However, I am very interested in other ways to convey this info, and to optimize the graph.
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I used EGFP as the protein fusion tag and CMV as the promoter of the plamid. I meet some problem when I try to assess the expression level of Cag-A in AGS cells. The EGFP protein is hard to visualize under microscope (almost none). And we next used western blot (anti-GFP antibody) and RT-PCR followed with agrose gel electrophoresis. The results of RT-PCR is positive while the WB is negative.
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There could be several reasons why you are having difficulty visualizing the EGFP protein under the microscope:
  1. Low transfection efficiency: If the transfection efficiency is low, then only a small fraction of the cells will express the EGFP-CagA fusion protein. This can make it difficult to detect the protein under the microscope. You may want to try optimizing your transfection protocol to increase the number of cells expressing the fusion protein.
  2. Low expression levels: Even if the transfection efficiency is high, the expression level of the EGFP-CagA fusion protein may be low. This can be due to a variety of factors, such as low transcriptional activity of the CMV promoter, inefficient splicing of the mRNA, or instability of the protein. You may want to try using a stronger promoter, optimizing the splicing of the mRNA, or stabilizing the protein to increase expression levels.
  3. Photobleaching: EGFP is sensitive to photobleaching, which can make it difficult to detect under the microscope. To minimize photobleaching, you can try using a lower excitation intensity or shorter exposure times when imaging the cells.
Regarding the negative western blot results, it is possible that the antibody you are using is not detecting the EGFP-CagA fusion protein. You may want to try using a different antibody or optimizing your western blot protocol to increase sensitivity. Alternatively, you could try using a different method for detecting the protein, such as immunofluorescence or flow cytometry.
These video playlists might be helpful to you:
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I tried to visualize the dendritic spines of dopaminergic neurons in ventrolateral periaqueductal gray (vlPAG) by injected retrograde virus AAV.rTH.PI.Cre.SV40 to Rostral ventromedial medulla (RVM) and also AAV5-CAG-FLEX-EGFP-WPRE to vlPAG (dilution ratio 1:2000) in C57BL6 mouse, then sacrificed at 3 weeks post-injection. However, I only can see the soma body and cant see dendritic spines. I wonder why I cant see the dendritic spines?
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Look for spines (ARC protein deposits) at locations where synchronous activation of neurons AND fine fibres of branched axons of other neurons are co-located, such as in the cerebral cortex.
I would not expect to see them where associative memory formation is not considered likely.
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Compare and contrast the different methods for assessing soil compaction, such as bulk density measurements, penetrometer tests, and visual assessments. Evaluate the strengths and limitations of each method, and their suitability for different soil types and land uses.
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Find the doc attached
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Hi everybody,
on a couple of occasions I used Phylogeoviz (phylogeoviz.org) to visualize haplotype distributions on a map. The last time I used it was about two years ago and it worked like a charm (thanks Dr. Tsai!). So, now I wanted to use it again for a fast and easy display. The page is still there, but it seems that it is not working anymore. The input is (mostly) accepted, but upon culculation, no map is displayed. I tried different browsers with the same result. Is it just (dumb) me, or is anybody else having the same problem?
Is there another easy way to visualize such date. I am sure that there are some GIS solutions, but as I said, I just need a quick display every few years and don't want to invest too much time.
Any suggestions?
TIA,
Klaus
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I'm having the same ploblem ;(
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İ am studying on visual perception of visually impaired users and designers. For sharing the act of designing experiencel, i am seeking for low-vision designers if there are any.
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Mahmut Ferit Aydin Mahmut, Not only are there are some….but there’s entire cultures who are more generous in avoiding such strict segregation (an either or) visually normal people from others.
I’m struggling to understand the assumption you make that there are only two; that is visually “normal” or visually impaired artists and designers. In my experience vision is made of physical, emotional, intellectual and “soulful” components. Together they form how we see and, in this context, how artists and designers strive to learn to “see”. In essence, I recommend (1) looking at vision as a continuous spectrum with each from their unique insights making contributions to art & design (2) not describing (only) one part of the visual spectrum as worthy of recognition for what it contributes to society. Oh, by the way, until we tested the vision of our children we thought they were intellectually impaired. Not simply in need of glasses. Same with the elderly!!!
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Why do we see the "down below" side of the physical world (e.g., the ground or the floor) at the bottom of our visual mental image (our visual perceptions)? Why not on the top of our mental imagery, for example?
But, are we even sure that the floor is at the bottom of our visual perception? Do we have even any reference that ensures us that the floor is at the bottom? Has our visual perception a bottom, to begin with? I guess NOT.
So what makes us think that the ground is at the bottom of our mental imagery?
I think this "top or bottom" thing of the visual perception is yet another Quale. So I guess we are back at the hard problem of consciousness again.
Note: I am not talking about cognition and evolutionary mechanisms that formed our cognitive 3D navigation mechanisms to register the ground as down below in order to be able to function consistently under gravity. I am talking about phenomenal consciousness.
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OK, so I write about this, the purely phenomenal level of our experience is a few true raw feels like pain and pleasure (Layer 1). Multiple instances of these plus information yield higher feelings, which are often called qualia (Layer 2), such as "the feeling that something (or myself or the focus of my attention) is "down" or "below" or at the bottom (which is also why my answer is more related to your question than might first appear). Information is involved, it is not pure feeling by the time you get to a typical quale, ie, you are already partly cognitive. Layer 2 is hardest one to understand, especially if you don't already accept the almost as hard Layer 1.
The feeling of down there is recall from somato-motor experiences. Everything about at-the-bottom is experience and brain wiring, nothing else.
The eye flipover thing is the brain synchronizing/calibrating arms and legs with vision.
Vision and somato-motor (kinesthesia-motor) are fused as you know. Internal abstraction of both gives a bottom sense to even quite abstract things.
So you see there is a reference- whatever we get form wiring and whatever we get from experience and whenever we move in space, that is cognitive information we acquire and not affective/phenomenal. If by your definitions cognitive requires active thinking then just call it cold information.
The only part of your mind that is information-free, zero cognitive are the most primitive glimmers of feeling. Everything else is built.
Your question is a nice example to use in explaining the Four Layer Architecture and in particular STFC.
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I am doing a repeated measures ANCOVA.
My independent variable is visual clutter measured as a between-subjects 2 levels categorical variable (Higher visual clutter versus lower visual clutter). For either the Higher visual clutter or lower visual clutter conditions, the participants in those respective groups either see the SPORT TYPE soccer first or basketball first (Note that everything is randomized so there are no order effects).
The dependent variable is consumer satisfaction and I have measured this separately for basketball and soccer. so two continuous dependent variables satisfaction (SATS) soccer and SATS basketball.
In the covariates box, I added the moderator: the continuous variable escapism and I added the interaction effect of visual clutter and escapism gender and age
My supervisor who is now unavailable told me in her feedback that if the difference in the means of sport type is significant I should include it as a covariate in the ANCOVA model.
But either I completely misunderstood her feedback or I am correct that it is impossible to create a dummy variable for a within-subjects continuous variable (sport type satisfaction).
And even so, in the repeated measures ANCOVA, the within-subjects test shows that there is no significant difference in means between basketball and football. But a simple paired t-test shows that for the higher visual clutter condition, there is a significant difference in means for the satisfaction levels between basketball and soccer while in the lower visual clutter condition there is not.
Can I drop sports-type based on the results of the repeated measures ANCOVA, the within-subjects effect being insignificant and conduct a regular ANCOVA afterwards?
My thesis is due in five days and I would deeply appreciate the help.
Sebastian Brom
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Did you find a way to create a dummy var for within subjects? I am running a mancova where each participant received 8 versions of a phishing email that combines 3 factors (A,B,C,AB,AC, BC, ABC, Control) and I am measuring the level of risk (4 levels). My covariates are age, gender and duration in the survey. I would like to know how to create a dummy variable that shows to my model the manipulation I am doing, but as all participants received all manipulations I am having some challenges to define my IV.
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Dear all, I am going to conduct online-studies about how sounds are represented visually by sketches.
Do you have an opinion, on what to consider when studying sketch-based associations of sound? (I do not want to go in the depth of my own conclusions, as I do not want to bias answers, or if someone wants to take part in the online study.)
What influence may have sketching types, or listening modes? If someone took part: What is your impression; is something "missing" to care about?
Many Thanks - cheers, lars
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you mean like a dynamic diagram in audio editor? Never mind :) Not really my field. These were not the droids I was looking for. I will move along.
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Do you recognize this planet? Is this a photo or a fantastic image of one of recently discovered and entirely covered with liquid oceans exoplanets?
This and other similar images will be used here to test our humans ability in recognizing visual patterns. Without (best as first guess) or with (final resort) help of AI. The conclusions may serve to enhancing AI tool for recognizing imaged objects.
(To enhance such alghoritms we may later discuss the way we found the solution without them.)
The first attached photo has already been solved a long time ago in one of RG precursors of this thread - nevertheless, I am recalling it in a slightly harder form (without description it contained at its bottom - as such was used to recognize the image by Aleš Kralj).
The rule is who will be first to recognize the planet (or other proponed image) (s)he will have right to demonstrate her/his own example of visual pattern to be solved.
Bjørn Petter Jelle commented once:
The planet thread is back with a lot of new questions and answers! :-) Unfortunately, all the old ones have gone somewhere into oblivion in the digital world of ResearchGate...?
And I'd answered: All is well what ends well. Isn't it?
Anyway, the actual format of RG questions is such that after few dozens answers all the previous also are vanishing in "eternal" oblivion, alike light in a Black Hole :-)
Sometimes, some information occurs again on its surface alike the quanta of Hawking radiation, however. And this new question is such new attempt of returning to our previous common experiments with human brain abiluty for recognizing of images or searching for them without and with refering to AI.
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This seemingly simple question doesn't have a simple answer. Everyone knows that Earth, Mars and Jupiter are planets. But both Pluto and Ceres were once considered planets until new discoveries triggered scientific debate about how to best describe them—a vigorous debate that continues to this day.
Regards,
Shafagat
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I am a starter of Quantum Espresso. I want to visualize the output files from Quantum Espresso. Primarily, I want to see atom configurations and trajectories from .xyz files and .path files. (these are output by NEB code)However, these files can not be read by Ovito.
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Hhhhhh
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Hello ! I am doing a research to identify the visual effects of Marketing on people with color blindness.
Colors red and yellow are known for stimulating appetite. Depends on the type of color blindness, we could have different effects on the affected people. How do I determine the effect ?
Thank you.
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You can use regression analysis.
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La idea es compartir experiencias del enfoque de la investigación de Gestión Visual, me pongo a disposición para platicar del tema, quedo atento a sus dudas o preguntas, saludos
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Los documentos a continuación no tratan específicamente sobre la gestión visual, pero mencionan el estándar de fábrica visual utilizado en la automoción con una breve descripción.
Espero que sean de alguna ayuda.
(PDF) Implementing Digitalised Lean Manufacturing Training in a UK Engine Manufacturing Centre During the SARS-CoV2 Pandemic of 2020 (researchgate.net)
(PDF) Improving Lean Manufacturing Systems and Tools Engagement Through the Utilisation of Industry 4.0, Improved Communication and a People Recognition Methodology in a UK Engine Manufacturing Centre (researchgate.net)
Atentamente
Martin
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Hello to everyone!
I am trying to understand an electrostatic issue. The scenario is, that we have a plate capacitor filled with vacuum (d=10cm), where a thin dielectric sample is placed on the bottom plate (ε=10, 1x1cm2 and 0,5mm thick). The applied voltages are -400V @ bottom plate and 0V @ top plate.
I simulated the problem with ANSYS Maxwell. Firstly, I visualized the E-Field around the sample (1) and secondly the E-Field on the dielectric surface itself (2).
(1) As you can see on the images, the E-Field shows edge effects leading to a reduced E-Field magnitude around the edges of the sample (green-blue), while above the center of the sample it is increased (yellow-orange).
(2) Inversely, looking at the sample surface itself, we can see that the E-Field in the center is weaker (dark blue) than at the edges (light blue-green). So the other way around...
I understand that the dielectric weakens the initial E-Field in general due to induced polarization. But what could be the reason for the lateral differences in E-Field magnitude between the edge areas (higher magnitude) and the center (lower magnitude)?
Does an increased E-Field in the edge areas of the dielectric surface mean, that there are more surface charges?
Thank you in advance!
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I don't think this means more surfaces charges because the simulation can only show the electric field distribution rather than the charge distribution. Usually the stronger intensity at edges is caused by the concentration of electric field lines(or vectors).
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Is there any way to better visualize that correlation with different colors
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I would visualize such a matrix with 3-D Surface or Contour plot and insert it in Word as a graph.
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  • How does the usability of the multimodal affect visitors' experience in heritage museum?
  • What are the implications of the use of multimodal for visitors' experience in heritage museum?
  • How to organise types of functions rather than specific features might be key to separate visual patterns from algorithms?
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please refer this link. some viewpoints can be founded.
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Hello! I am currently writing my first paper to be published, and I would love some advice on how to explain the statistical analysis of my experiment. For my study I grew bacteria in two cell lines, with and without cycloheximide (4 treatment groups total). I then harvested the flasks for 14 days and quantified the bacteria in my samples. I grew each flask in duplicate and tested them in triplicate, so I ended up with 6 data points per treatment group per day.
I averaged these data points to create a line graph to visualize growth, and I used excel to perform ANOVA using 2 treatment groups and 1 harvest day at a time, to see if they were significantly different on each day. I need to briefly describe this in my methods section, but I am having trouble wording it in a professional way, as it was a pretty simple process.
Any advice would be appreciated! Thanks!
Alex H
CDC ORISE Fellow
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The within factor refers to the repeated measures
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Hello everyone
I've been having trouble getting results for a particular set of primers, but I can't get any bands, so I decided to do a contact PCR, but the result was the same, no visual bands at all.
I used 25 runs with a starting temperature of 67°C to 55°C, reducing 0.5°C per run, and different samples from different strains of the organism my primers target.
I will appreciate your help :)
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Joseph Japhet, Paul Rutland Thank you so much both I will try and let you know the results soon :)
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Watching movies and interacting with visual media are engaging ways to help students learn.
So, I would like to stream a movie, TV show, or documentary as part of a lesson for undergrad students in a supply chain course.
Do you suggest any media that could be useful?
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Arriving Today: From Factory to Front Door written by Christopher Mims inspired for doing a documentary that you can find on youtube: Why Global Supply Chains May Never Be the Same
another didactic suggestion: try to make students play a fiction interview between oneanther, as if they really touched the supply chain 's jobs, and how would they imagine their different situations and their reactions
Bests
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I created this R package to allow easy VCF files visual analysis, investigate mutation rates per chromosome, gene, and much more: https://github.com/cccnrc/plot-VCF
The package is divided into 3 main sections, based on analysis target:
  1. variant Manhattan-style plots: visualize all/specific variants in your VCF file. You can plot subgroups based on position, sample, gene and/or exon
  2. chromosome summary plots: visualize plot of variants distribution across (selectable) chromosomes in your VCF file
  3. gene summary plots: visualize plot of variants distribution across (selectable) genes in your VCF file
Take a look at how many different things you can achieve in just one line of code!
It is extremely easy to install and use, well documented on the GitHub page: https://github.com/cccnrc/plot-VCF
I'd love to have your opinion, bugs you might find etc.
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I use TASSEL software for genome analysis. You need plink format of map and pad to operate it. You can try and explore this software
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I'm trying to plot solar irradiance or GHI from WRF output. Is there any python script to visualize this ?
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Naveen Venkat Ram Kumar SWDOWN stands for surface downward shortwave radiation, which is the quantity of solar radiation received by the earth's surface. In Python, you can visualize this data from WRF output by using a library like xarray to read in the WRF output data and then a library like Matplotlib or Cartopy to construct the visualizations.
Here's some Python code that shows how to use xarray and Matplotlib to view SWDOWN data from WRF output:
import xarray as xr
import matplotlib.pyplot as plt
# Open the WRF output file using xarray
ds = xr.open_dataset('wrfout.nc')
# Extract the SWDOWN data from the dataset
swdown = ds['SWDOWN']
# Select a single time step to plot
swdown_slice = swdown.isel(time=0)
# Plot the SWDOWN data using Matplotlib
plt.figure()
swdown_slice.plot()
This code will use xarray to load the WRF output file, extract the SWDOWN data from the dataset, choose a single time step to plot, and finally plot the data with Matplotlib. You can change the code to plot additional time steps or to tweak the plot's look as desired.
I hope this was helpful! Please let me know if you have any queries or need any further information.
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I work with ipsc-CMs and we are trying to measure the action potential of the cells with Fluovolt. After preparation and incubation as described by the manufacturer, we were able to visualize the signal peak before the contraction. But after recording cells for a couple of minutes (1-2min), the frequency decreases along with fluorescence reduction until the cells stop completely and we can no longer see any signal (baseline fluorescence continues). We observed that this problem starts right after fluovolt excitation, as it does not occur in bright field (end of contraction).
A few notes> we add Blebbistatin (10uM) in the incubation solution but we already tested without it. We also tested 3 different dilutions of fluovolt and different light intensities and the problem remains.
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With high settings of the excitation light (> 30–40% of Sola output) the dye had high phototoxicity and caused 100% cell death within minutes after a 30–60 s illumination (data not shown). Damaged cells “retaliated” by facilitating dye bleaching (possibly by releasing ROS) far beyond the bleaching rate in intact cells, the effect which could be misinterpreted for hyperpolarization. Fortunately, the phototoxicity was not noticeable at low light settings (< 10% of Sola output) and in practice less than 5% of the light output was adequate even for the fastest imaging (Pakhomov AG, Semenov I, Casciola M, Xiao S. Neuronal excitation and permeabilization by 200-ns pulsed electric field: An optical membrane potential study with FluoVolt dye. Biochimica et Biophysica Acta (BBA)-Biomembranes. 2017 Jul 1;1859(7):1273-81).
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As far as I read from surveyings, papers & documentations of some libraries, it seems like each pixel in the data array part of a DICOM slice (each .dcm file) is just HU value (in radiology terminology).
Since I tried to open many pixel_array using Pydicom or Mathlab, it is integer with the range is really high like [0, 2000] or more.
I'm not sure I was correct about this because I'm still wondering how each slice is visualized? (because normal image is in [0, 255] scale) ? ( or they scale down from HU range to [0,255] ? )
OR I was wrong, the CT scanner output the slice with normal image [0, 255] after processing the HU already ?
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To visualize each slice, window\level operation is applied. Refer here for more details: https://kevalnagda.github.io/ct-windowing .
So, changes of 'window' value leads to image contrast change, and changes of 'level' value leads to image brightness change.
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I am studying the visual analysis of diagrams. In that context I need to find the relation of attention with sensation and perception. I think attention is related to both sensation and perception, but I would like to find articles dealing with this subject that could clarify my doubts.
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There is a continuum between sensation and attention. How you mix it up determines the kind of perception you have (e.g. observation vs. recognition). I worked out a theory based on quantum physics that extrapolates to a metaphysical description of how we operate. You can find it at primalword.com
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In theory clasees, we all have learnt about drainage density (DD), which is the ratio of 'total length of stream network in a basin (L)' to the 'basin area(A)'. However, when, we are trying to determine DD of some Indian river basins using Arc-GIS, we are facing some confusions. For instance,
1. We have to define some particular break value in Arc-GIS to get streams of differnt orders. Lesser the break value, higher is the stream order, therefore resulting in a higher value of L, and vice versa. For example, when we set the breakvalue as 500 for a particular basin, we are obatining a stream order upto 7, but if we increase the break value to 1500, the max. stream order reduces to 5 - automatically the L value also reduces. Thus, the same baisn may yeild two differnt DD values, under two aforesaid considertions of break values.
2. We also fixed the theoretically least possible break value , i.e., >0 and obtained a extremely dense stream network with high DD value.
So, my question is, what should be the threshold break value for a particular basin in order to get the DD?
3. From literature, we found that, there are five classes of DD with the following value ranges (km/km2), i.e., very coarse (<2), coarse (2-4), moderate (4-6), fine (6-8), and very fine (>8). However, for 20 river basins across differnt parts of India, we obtained DD values ranging between 1.03 to 1.29, which makes all those basins fall under very coarse category. But, from our visual inspection (one sample bain attached below), it seems to be very less to us.
We want some justification/ clarification/ comment on it.
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Dear Victor Hugo Burgos, I completely agree with you. I guess overlapping the developed stream network with some base map (e.g. google terrain map) might serve the purpose to identify where the river begins. However, as we are dealing with rain-fed river basins, where most of the times, the lower order streams (1st or 2nd) are dry, it becomes bit difficult to identify them as stream from the base map. Nonetheless, its a very good suggestion, and thanks a lot for that.
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Hi everyone. I'm planning on determining MP presence, size, color, shape, etc., in other words, in doing a visual sorting/characterization of MP accumulated in penaeid shrimp abdominal muscle. Nevertheless, visual sorting becomes more difficult as particle size get smaller, and is time-consuming and is more likely to fall into misidentification errors. Generally, it is recommended to do visual sorting with plastics no less than 500 microns, but I'm anticipating that any plastic embebed in the abdomen is much smaller than that. I was planning to try alcali tissue digestion with KOH and fiber glass microfilters of 2 microns of pore size, and my intention was to observe the filters under a stereoscopic microscope of a minimum of 45X of magnification. But still I'm going to obtain small plastic particles, if any (spoiler: there will be). So my question is if you have any recommendation or alternative method?... observe the filters under a fluorescent microscope using Nile red to facilitate MP discrimination? analyze another tissue? use a greater pore size filter? change the organism... or maybe it is possible to do the job. Espectroscopy methods are not allowed, since it is part of another stage of the project, I just wanna perform visual sorting/characterization.
Thank you very much for your attention.
Best regards
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You are correct, visual sorting gets increasingly difficult as the particle size gets smaller. The sizes of MPs that you are able to pick out of your sample first comes down to what you can see, and that is often dependent upon the magnification abilities of your microscope. And there can be a fair amount of error associated with that as MPs often look like other things (e.g., diatoms). Adding additional techniques before visualization can help a lot.
First is the digestion of the tissue. I have tried both KOH and H2O2 + heat on fish tissues and found them both to be effective. I typically use H2O2 at 65C for a few of hours with periodic agitation, depending on the size of the tissue sample. Karami et al. (2017) has a nice paper on different types of digestions. Next is separation from the surrounding media. If you are interested in separating by polymer type, then you can consider a density separation. Li et al. (2018) provides a good method. Just know that some of the chemicals used can be a little difficult to handle and particle size can impact buoyancy. The latter might be solvable by adding centrifugation (see Nguyen et al. 2019). You mention filtration, and I would say that is the most common method. There is some discussion about how to best filter samples to get the most MPs while avoiding contamination. While not the only one, Cai et al. (2020) addressed that subject recently. Personally, I think that filters are a good way to go if your MPs are large enough to be caught by it. You should consider passing the digestate and subsequent filtrates through multiple filters with smaller and small pore sizes so that you you don’t clog filter pores and when you get to the smallest particles large bits aren’t obscuring the view of smaller particles. Nanoplastics are still a big problem. The Nguyen et al. (2019) study says that their technique is able to separate those too, but I haven’t tried it yet. Its generally agreed upon (as of now) that there is no one good method to separate out the really small nanoplastics. And if you think you have a separation method, once they get that small, the only way to verify if you got any is by using an electron microscope (maybe uFTIR…very much maybe). That’s one of the reasons most people purchase fluorescent NPs to use in their exposure experiments. Next is the Nile red staining that you mentioned (I’m assuming you are using protocols from Maes et al. 2017 and Shim et al. 2016?). I certainly see this as one of the more commonly used methods to differentiate MPs from their background. And, if your microscope has enough resolution, you should be able to see particles <500um. Considering that you are using shrimp tissue, you should determine if you will get autofluorescence within the same wavelengths as the stain. I also recommend reading Meyers et al. (2022); they have some interesting ideas about using Nile red that I look forward to trying. Stanton et al. (2019) proposes the use of DAPI as a costain gives better results. And as the previous responder mentioned, FTIR has the final say in whether something is a plastic or not, and what kind it is. If it is possible for you to do on at least a subsample of what you separate from your sample, then it will make your study stronger. Regarding tissue type, I think that has more to do with your question. When dealing with aquatic organisms, exposure route should be carefully considered as it can be inhalation, dermal, and/or ingestion. Particle size typically determines if an how a particle can translocate through the body, and not all tissue types are equally permeable. The muscle seems like generic sort of tissue to look at, not in a bad way though. Would it be possible to collect hemolymph?
I’m not sure how much I helped to solve your problem, but I hope I at least gave you a few more directions to look in.
Good luck!
- Melissa
Cai, H., et al. (2020) Microplastic quantification affected by structure and pore size of filters. Chemosphere 257, 127198. http://doi.org/10.1016/j.chemosphere.2020.127198
Nguyen, B., Claveau-Mallet, D., Hernandez, L. M., Xu, E. G., Farner, J. M., & Tufenkji, N. (2019). Separation and analysis of microplastics and nanoplastics in complex environmental samples. Accounts of chemical research, 52(4), 858-866. https://doi.org/10.1021/acs.accounts.8b00602
Karami, A., et al, (2017) A high-performance protocol for extraction of microplastics in fish. Science of the Total Environment 578, 485-494. http://doi.org/10.1016/j.scitotenv.2016.10.213
Stanton, T., et al. (2019). Exploring the efficacy of Nile red in microplastic quantification: a costaining approach. Environmental Science & Technology Letters, 6(10), 606-611. https://doi.org/10.1021/acs.estlett.9b00499
Meyers, N., et al, (2022). Microplastic detection and identification by Nile red staining: Towards a semi-automated, cost-and time-effective technique. Science of the Total Environment, 823, 153441. https://doi.org/10.1016/j.scitotenv.2022.153441
Li, L., et al., (2018). A straightforward method for measuring the range of apparent density of microplastics. Science of The Total Environment 639, 367-373. http://doi.org/10.1016/j.scitotenv.2018.05.166
Maes, T., et al. (2017) A rapid-screening approach to detect and quantify microplastics based on fluorescent tagging with Nile Red. Scientific Reports 7, Article number: 44501. http://doi.org/10.1038/srep44501
Shim, W.J., et al. (2016) Identification and quantification of microplastics using Nile Red staining. Marine Pollution Bulletin 113, 469-476. http://doi.org/10.1016/j.marpolbul.2016.10.049
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In R Programming how to visualize time data ? as this: https://github.com/vasturiano/timelines-chart
thanks in advance
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Try Gantt charts in ggplot
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I want to visualizing the temporal relationship of patients records over time, assuming that i have EHR dataset, what are the used techniques to plot or visualize such kind of dataset, where each patient have different features which are updated over time.
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Thank you for your answers, really appreciated.
I want to visualise multiple clinical variables ( temperature , blood pressure, sugar… ) for multiple patients, recorded in multiple clinical visits by time.